Status
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Gene information
General information
TC000780 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5546909..5554515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000780
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08986

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 2

    vitellarium size decreased - Number of animals showing the phenotype: 2
    egg chamber number decreased - Number of animals showing the phenotype: 3
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3
    mature egg not present - Number of animals showing the phenotype: 3
    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 1
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 1

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000780 RA
      MWTVVISGC VSFSLVMSL AFCLCWRKK SPKNDLLGL 
      DGMVIQKNP DENVNHLPS AAPCVVDGV NASANSADF 
      NDSNNKRNI PANNTRRSL PDIPSEQGA ALANWEPTG 
      DNSSEHYAT VGQYQNAAK RHTLQNGVE PRPSLSQHS 
      SITQADDNS SPYEKVKYD KIKSKEHPY AQLQPTTSR 
      QAALEENKL VGEERTNLL RPSGSSTSS EPSAPPRSR 
      RSSAHSGLG VDIPAASAV AGGIAASPE LPYMTPPVT 
      QPNFSGDSQ DSSKGYTSI SVREPLANI IAQTKEMNK 
      KRDIDQHYS TVSDDSDDV YTTIPDPNN QIYTSESET 
      YARIPAHPI TVEAEVNPT PRSLETDEF YEPAPQPPP 
      PSVTSLKQV AGHSHSRQA SSSSSIANI GSPKPEKRQ 
      ANSPLPPPP PGSSFDFQR NLDDLYAKV HKNKKKEDS 
      DGEKTPEKA KRSGDNTSR KSVSSCSST EEKKFLPTS 
      RHQEHNYET LRKSNKDCS DPGYEKIRN EEPGYASIN 
      GPESITSSD PGYEVLKQR PLSELDPNY EELKHRNSN 
      ASDSGYSKV KYTDGYSVV NKQKSDDKS RTSSSYGSN 
      SQDFSLDEP NYESMPSES LSSEHNYAA LKSGGSESD 
      PNYESVRDL EEPPYEKLN DGDSDFQVS GRGYENIDT 
      SESSNGAGD TKSQKSGPP YEHLNNDTD SEVPGYERI 
      KGKENASSG ESSSSGANK GASILEEDA IFQV
    CDS sequence
    • >TC000780 RA
      atgtggaca gttgttatt tcggggtgt gtgtctttt 
      tcactggta atgtcgtta gcgttttgt ctgtgctgg 
      aggaagaag tcgcctaaa aatgacttg ttagggctt 
      gatggcatg gtcattcaa aaaaaccct gacgaaaat 
      gttaatcac ttgccctct gctgcacca tgtgttgtt 
      gacggcgtg aatgctagt gcaaatagt gctgacttt 
      aacgattct aataataaa aggaacatt cctgccaac 
      aatacgcga agaagtctc cctgatatt ccctcagaa 
      caaggggct gctctcgct aattgggaa ccaacaggt 
      gacaattcc tccgaacat tacgccact gttggccaa 
      tatcaaaat gcagcaaaa cgtcacacg ctccaaaac 
      ggtgtggag ccccgtcca agtctatct caacacagt 
      tcaatcact caagccgat gataattcc tcaccttac 
      gaaaaggtg aaatatgat aaaatcaaa tcaaaagaa 
      cacccttac gcgcaatta cagccgacg acaagccga 
      caggcagct ttggaagaa aataaactg gttggagag 
      gagagaaca aacttgttg agacctagt ggttccagc 
      acatcgtca gaaccttca gcacctcca cgatcacga 
      agatcgtcc gcacacagt ggactagga gtggatatt 
      ccggcggct tcagccgtt gctggaggt attgctgct 
      agtccagaa ctgccttat atgactccg cccgtaaca 
      cagcccaat tttagcggg gattcacag gattcttcg 
      aagggttac accagtatt agtgtgaga gagccgtta 
      gctaatatt atagcacag acaaaagaa atgaacaaa 
      aagagggac atagaccaa cattattcc acggtgtct 
      gatgattca gacgatgtt tatacgacg atccccgac 
      cccaacaac caaatctac acgagcgaa agcgaaacc 
      tacgcccga attcccgct catccgata accgtcgaa 
      gccgaagtc aatccaact cctcgaagc ctcgaaacc 
      gacgaattt tacgaacct gcaccacaa ccaccgccc 
      cctagtgtc accagtttg aagcaagta gctgggcac 
      tcccactcc cgacaagct tcatcgtcg agttccata 
      gccaacatt ggctctcct aagccggaa aaacgccaa 
      gcgaactca cccctacct ccaccaccc cctggttca 
      agtttcgat ttccagcga aatttagat gatctctac 
      gccaaagta cataaaaac aaaaagaag gaagattct 
      gatggggag aaaaccccc gaaaaggca aaacgtagt 
      ggcgataat acaagtcgt aagtcagta tcgagttgc 
      agttcaacc gaagaaaag aaatttctt ccgacatca 
      agacaccaa gaacacaat tacgaaaca ttacgcaaa 
      tcaaataaa gattgtagt gatccaggc tatgagaaa 
      attcgaaat gaggagcca ggctatgcc agtattaac 
      ggcccagag agcataacg agctcagac ccgggctac 
      gaggtttta aaacagcgg cctctatcg gaattggac 
      cccaactac gaggaactc aaacatcga aattcgaac 
      gctagtgat tccggttat tcgaaagtc aaatacact 
      gacggttat tcggtggtt aataagcaa aaaagtgac 
      gataaatcg cggacgtca agtagttac ggcagtaat 
      tcgcaggat tttagttta gacgaaccg aactacgaa 
      agtatgccg agtgaatcc ttgtcttcc gaacacaat 
      tacgcggct ttgaaatca ggcggaagc gaatcggat 
      ccgaattat gaatcggtg agggatttg gaagagcct 
      ccgtatgaa aaattgaac gatggtgat tcggatttt 
      caagtatca gggcgtgga tatgagaat attgacact 
      tcggagagt tcgaatggg gctggagat acgaaatct 
      caaaaatct gggccgcct tatgaacac ttgaacaac 
      gatacggat tcagaggtg cctgggtat gaacgcatc 
      aaaggcaag gaaaatgcg tcaagtggg gagtcaagc 
      agttcgggg gctaataag ggggcgagt atacttgaa 
      gaagatgct atttttcaa gtctaa
    mRNA sequence
    • >TC000780 RA
      gtttgatgg acctggcgt gcggacaat ttttatttt 
      gttttgact tgtcacatt tgacatttt gagcttggc 
      aaatgtgtt gtgagcagt tgaggtgtt tgtttgttt 
      tttgtgaac tggtgaaag ccacattaa taaaagtcg 
      attactttt gccaaatcc tgaatttgg gcaaaattt 
      tgtcggtgt tgaataatg ctggacgtt gttcctagt 
      tgtgccact taggtgtga ccctattaa cctgtcgtc 
      aagctctcg gttaatcga acatgatta agaaaatat 
      cgcacttag tggcaatga aacgtaaag ttaggctca 
      acatcacaa aaatgtaaa acccagttt aaagacatg 
      tggacagtt gttatttcg gggtgtgtg tctttttca 
      ctggtaatg tcgttagcg ttttgtctg tgctggagg 
      aagaagtcg cctaaaaat gacttgtta gggcttgat 
      ggcatggtc attcaaaaa aaccctgac gaaaatgtt 
      aatcacttg ccctctgct gcaccatgt gttgttgac 
      ggcgtgaat gctagtgca aatagtgct gactttaac 
      gattctaat aataaaagg aacattcct gccaacaat 
      acgcgaaga agtctccct gatattccc tcagaacaa 
      ggggctgct ctcgctaat tgggaacca acaggtgac 
      aattcctcc gaacattac gccactgtt ggccaatat 
      caaaatgca gcaaaacgt cacacgctc caaaacggt 
      gtggagccc cgtccaagt ctatctcaa cacagttca 
      atcactcaa gccgatgat aattcctca ccttacgaa 
      aaggtgaaa tatgataaa atcaaatca aaagaacac 
      ccttacgcg caattacag ccgacgaca agccgacag 
      gcagctttg gaagaaaat aaactggtt ggagaggag 
      agaacaaac ttgttgaga cctagtggt tccagcaca 
      tcgtcagaa ccttcagca cctccacga tcacgaaga 
      tcgtccgca cacagtgga ctaggagtg gatattccg 
      gcggcttca gccgttgct ggaggtatt gctgctagt 
      ccagaactg ccttatatg actccgccc gtaacacag 
      cccaatttt agcggggat tcacaggat tcttcgaag 
      ggttacacc agtattagt gtgagagag ccgttagct 
      aatattata gcacagaca aaagaaatg aacaaaaag 
      agggacata gaccaacat tattccacg gtgtctgat 
      gattcagac gatgtttat acgacgatc cccgacccc 
      aacaaccaa atctacacg agcgaaagc gaaacctac 
      gcccgaatt cccgctcat ccgataacc gtcgaagcc 
      gaagtcaat ccaactcct cgaagcctc gaaaccgac 
      gaattttac gaacctgca ccacaacca ccgccccct 
      agtgtcacc agtttgaag caagtagct gggcactcc 
      cactcccga caagcttca tcgtcgagt tccatagcc 
      aacattggc tctcctaag ccggaaaaa cgccaagcg 
      aactcaccc ctacctcca ccaccccct ggttcaagt 
      ttcgatttc cagcgaaat ttagatgat ctctacgcc 
      aaagtacat aaaaacaaa aagaaggaa gattctgat 
      ggggagaaa acccccgaa aaggcaaaa cgtagtggc 
      gataataca agtcgtaag tcagtatcg agttgcagt 
      tcaaccgaa gaaaagaaa tttcttccg acatcaaga 
      caccaagaa cacaattac gaaacatta cgcaaatca 
      aataaagat tgtagtgat ccaggctat gagaaaatt 
      cgaaatgag gagccaggc tatgccagt attaacggc 
      ccagagagc ataacgagc tcagacccg ggctacgag 
      gttttaaaa cagcggcct ctatcggaa ttggacccc 
      aactacgag gaactcaaa catcgaaat tcgaacgct 
      agtgattcc ggttattcg aaagtcaaa tacactgac 
      ggttattcg gtggttaat aagcaaaaa agtgacgat 
      aaatcgcgg acgtcaagt agttacggc agtaattcg 
      caggatttt agtttagac gaaccgaac tacgaaagt 
      atgccgagt gaatccttg tcttccgaa cacaattac 
      gcggctttg aaatcaggc ggaagcgaa tcggatccg 
      aattatgaa tcggtgagg gatttggaa gagcctccg 
      tatgaaaaa ttgaacgat ggtgattcg gattttcaa 
      gtatcaggg cgtggatat gagaatatt gacacttcg 
      gagagttcg aatggggct ggagatacg aaatctcaa 
      aaatctggg ccgccttat gaacacttg aacaacgat 
      acggattca gaggtgcct gggtatgaa cgcatcaaa 
      ggcaaggaa aatgcgtca agtggggag tcaagcagt 
      tcgggggct aataagggg gcgagtata cttgaagaa 
      gatgctatt tttcaagtc taaagtcgt gcctacaat 
      ctggtgcta agcaatttt ataccaatt cattatttt 
      caaacagtg atatagtta cttttaaga gattctctt 
      ttgtagaaa ttctatttt gatgtcgtg attactgat 
      aggtagggt tttacaatt tttcaacga aaagtgaag 
      catgtatac gtattaaac aattttgta tgtgttatt 
      gcatttatg ttctcattt gtcttccgt atgatcatt 
      tcatttgcc ctgtgccaa ataggtact cagtacttt 
      ttatgttac atgtgttta caatagact gcttttaga 
      ggttttaat gttatttta gttacaaaa ttgttgcaa 
      tattaaaga ttaatatat tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY