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Gene information
General information
TC004570 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2184519..2201143
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004570
    iBeetle screen iB_08969

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 2

    vitellarium shortened - Number of animals showing the phenotype: 2

    vitellogenic egg chamber potentially position anteriorized - Number of animals showing the phenotype: 2

    previtellogenic egg chamber potentially not present - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004570 RA
      MASEETSNV SERGKTEKN KNYNPKLDF FSEQFDPLL 
      ALRTPNVTI PVPNAKTHD NIHRFKSVL EGKTQAPVK 
      SKKTEEPQT APVERRWLP HQLPIQTKR KTFYRNIFV 
      RMENAVGPL QLLKTCREK RHRIKIWTR HSHDIRGYC 
      LAYVVAFDK HWNLALEDV EEVWTRPKK RKIPALDST 
      RKLEIKPKV LPPPITIIQ STKKTETCS RHVSQLLLR 
      GEHIAFIVV VPNVGLERF PKKHKVRLC KAVIFRKKC 
      RVQQLRGEF SVLT
    CDS sequence
    • >TC004570 RA
      atggcctcc gaagaaacg tcaaacgtt agtgaacga 
      ggaaaaacc gaaaaaaac aagaattac aacccgaaa 
      ttagacttt ttcagcgaa cagttcgat ccactgttg 
      gctttacga acaccgaac gtaactata ccggttcct 
      aatgcaaaa acgcacgat aatattcac cgatttaag 
      tcagtgctc gagggtaaa actcaagca ccggttaaa 
      agcaaaaaa accgaagag ccccaaact gctccggta 
      gaaagacgg tggttgcca catcagtta cccattcag 
      accaaaaga aaaacgttt tacagaaac atatttgtg 
      agaatggaa aacgccgtt ggaccttta cagctcctc 
      aagacttgt agggagaaa cggcataga atcaagatc 
      tggacgcga catagtcat gacattcga ggatattgc 
      ctggcgtat gttgtcgct tttgataag cattggaat 
      ctggcatta gaagacgtt gaagaagtg tggacgagg 
      ccgaagaaa cggaaaata ccagcgctt gatagtaca 
      agaaaacta gaaattaag ccgaaggtc ttaccaccg 
      ccgattact ataatacag tcaacaaaa aagacagaa 
      acttgctca aggcatgtc agtcagctt ttgctccga 
      ggtgaacat attgccttt atagttgta gttccgaat 
      gtgggcttg gagcggttc ccgaaaaag cacaaggtt 
      agattatgc aaagccgtt atatttcgg aaaaagtgc 
      cgggttcaa caacttcgg ggcgagttt tccgtcctc 
      acctga
    mRNA sequence
    • >TC004570 RA
      ttgtttaaa aatggcctc cgaagaaac gtcaaacgt 
      tagtgaacg aggaaaaac cgaaaaaaa caagaatta 
      caacccgaa attagactt tttcagcga acagttcga 
      tccactgtt ggctttacg aacaccgaa cgtaactat 
      accggttcc taatgcaaa aacgcacga taatattca 
      ccgatttaa gtcagtgct cgagggtaa aactcaagc 
      accggttaa aagcaaaaa aaccgaaga gccccaaac 
      tgctccggt agaaagacg gtggttgcc acatcagtt 
      acccattca gaccaaaag aaaaacgtt ttacagaaa 
      catatttgt gagaatgga aaacgccgt tggaccttt 
      acagctcct caagacttg tagggagaa acggcatag 
      aatcaagat ctggacgcg acatagtca tgacattcg 
      aggatattg cctggcgta tgttgtcgc ttttgataa 
      gcattggaa tctggcatt agaagacgt tgaagaagt 
      gtggacgag gccgaagaa acggaaaat accagcgct 
      tgatagtac aagaaaact agaaattaa gccgaaggt 
      cttaccacc gccgattac tataataca gtcaacaaa 
      aaagacaga aacttgctc aaggcatgt cagtcagct 
      tttgctccg aggtgaaca tattgcctt tatagttgt 
      agttccgaa tgtgggctt ggagcggtt cccgaaaaa 
      gcacaaggt tagattatg caaagccgt tatatttcg 
      gaaaaagtg ccgggttca acaacttcg gggcgagtt 
      ttccgtcct cacctgaaa tccatgtaa tttaaacgt 
      atccaacat gtaatccac actgaaaaa aaagcactg 
      cactgaaca ccacacaaa gtatgattg tagtttcga 
      gtaataaaa ataattcca ggcacaatt gggtcaggc 
      catttacgg ctccagggc ccgtcagcg gttctagtc 
      aagtcaggt caccaaaca gcatccaaa aagccacag 
      cacttagca cccaattgc gagcataga taaccacga 
      cgaaagtac acacaacac tcggatccc caactcgat 
      aatacaatt taccgattt gtttgtttg ggttaacgg 
      tgaaattca agtttggaa ctggcgctc accgtcaga 
      gagcgtgcg caagtcgaa ggcgcgctc gcaagctaa 
      acactaagt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY