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Gene information
General information
TC004818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2344053..2347324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004818
    iBeetle screen iB_08968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004818 RA
      MAILTYSWL LDIFGILTA LIAVFIAWA QWSHTYWSR 
      RNLIAPKTT FFLGNSKGI VTQKQSFGD FTVEMYEYI 
      RKHNKPHGG FYLTLMPNY MPVDPEIIK HIMLNDFDH 
      FVDRGIYYN EEVDPLSAH LFSLDGAKW RNLRIKLTP 
      TFTSGKMKM MFDTLVKCS DQLLVEMNQ TAGKAPVDA 
      KNILARFTT DIIGSCAFG IECNSLKNP NDEFTQYLK 
      LFFVEGFWQ NLNGIITFV APELAKKLN MKVVNPVLS 
      NFFMKVVED TVNMRERNN IYRKDFMHL LLQLKNRGE 
      LVDDDSILQ NNTDAENKE VTLTLNELA AQAFVFFLA 
      GYETSSTTM TFCLYELAS NPEIQDKLR AEINEVLQK 
      HGKLTYDAI MEMRYMDKV VNETLRKYP PLPGLNRVC 
      NKEYKVPGT DFVIEKGTK IWIPVLGLH RDPEYFPNP 
      EKFDPERFT EENKRQRHP YTYLPFGEG PRICIGLRF 
      GMMQTKVGL SVLLKNYKF SINSKTKLP LQLDPKSFI 
      MSTEGGIWL DYTRV
    CDS sequence
    • >TC004818 RA
      atggcgata ctaacgtac agttggtta ttagacatt 
      tttggcatc ttgacggca cttattgct gtttttatt 
      gcttgggca caatggtct cacacttac tggtctcgt 
      agaaatctt atagcccct aaaacgacg ttcttcctc 
      ggcaacagc aaaggaatc gtcacacag aaacaatcc 
      ttcggagat ttcaccgtc gaaatgtac gaatacatc 
      cgcaaacac aacaaacct cacggtggt ttctacctc 
      acccttatg cctaattac atgcctgtt gatcccgaa 
      atcatcaaa catatcatg ctgaacgat ttcgaccac 
      ttcgtagat cgaggaatt tactacaac gaagaagtc 
      gatccgtta agtgcccat ttgttctct ctggacggt 
      gctaaatgg cggaattta cgaatcaag ctcacacca 
      actttcacg tcgggaaaa atgaaaatg atgtttgat 
      actttggtg aagtgtagc gaccagttg ttggtggag 
      atgaaccaa acagctggg aaagcccca gttgatgca 
      aaaaatata ctagcacgg tttacaacc gacataatt 
      gggtcttgt gctttcggg attgagtgc aactcgctg 
      aaaaacccc aacgatgag ttcactcag tatttaaaa 
      ctgtttttc gtggaaggt ttttggcaa aacctcaac 
      ggaataatc actttcgtg gcgcccgag ttggccaaa 
      aaactgaac atgaaagta gtcaaccct gtcctgtcc 
      aattttttc atgaaagtg gtcgaagac accgtcaat 
      atgcgcgaa cgcaacaac atctaccgc aaagatttc 
      atgcatctc ctgctccag ctgaaaaat cgaggggaa 
      cttgttgac gacgacagc atcctacaa aacaatacc 
      gatgccgaa aataaggaa gtgacgctg actttgaac 
      gagctcgca gcccaagct ttcgtcttc ttcctggcc 
      ggttacgag acctcctcc accactatg acgttttgc 
      ttgtacgaa ttagcgtcg aatcctgaa attcaggac 
      aaattgcgc gctgaaata aacgaagtt ttgcaaaag 
      cacggcaaa ttgacgtat gacgcaatt atggaaatg 
      cgttacatg gacaaagtc gtgaatgaa actctacgc 
      aagtatccg ccccttccg ggcctgaac cgcgtctgc 
      aacaaggaa tacaaagtc cccggaaca gattttgtg 
      attgaaaag gggacgaaa atctggatt ccggtgctg 
      ggtttgcac cgggacccg gagtatttc ccaaatccg 
      gagaaattc gacccggag aggttcacg gaggagaac 
      aagaggcag agacaccct tatacgtat ctacccttc 
      ggcgaagga cctaggatt tgcattggt cttaggttc 
      gggatgatg caaactaaa gtcgggctt agcgtcctt 
      ttgaagaat tacaagttt tccattaat tccaaaacc 
      aaactgccg ctgcaactt gaccccaaa agcttcatc 
      atgagcaca gaaggcggg atttggctt gattatacg 
      cgcgtttaa
    mRNA sequence
    • >TC004818 RA
      ttagttaaa taacaaatg tattagaat ttatcttat 
      tagtgatgt tttattgaa taaacagaa tatttttta 
      agaaaaaaa aatataatg gcgatacta acgtacagt 
      tggttatta gacattttt ggcatcttg acggcactt 
      attgctgtt tttattgct tgggcacaa tggtctcac 
      acttactgg tctcgtaga aatcttata gcccctaaa 
      acgacgttc ttcctcggc aacagcaaa ggaatcgtc 
      acacagaaa caatccttc ggagatttc accgtcgaa 
      atgtacgaa tacatccgc aaacacaac aaacctcac 
      ggtggtttc tacctcacc cttatgcct aattacatg 
      cctgttgat cccgaaatc atcaaacat atcatgctg 
      aacgatttc gaccacttc gtagatcga ggaatttac 
      tacaacgaa gaagtcgat ccgttaagt gcccatttg 
      ttctctctg gacggtgct aaatggcgg aatttacga 
      atcaagctc acaccaact ttcacgtcg ggaaaaatg 
      aaaatgatg tttgatact ttggtgaag tgtagcgac 
      cagttgttg gtggagatg aaccaaaca gctgggaaa 
      gccccagtt gatgcaaaa aatatacta gcacggttt 
      acaaccgac ataattggg tcttgtgct ttcgggatt 
      gagtgcaac tcgctgaaa aaccccaac gatgagttc 
      actcagtat ttaaaactg tttttcgtg gaaggtttt 
      tggcaaaac ctcaacgga ataatcact ttcgtggcg 
      cccgagttg gccaaaaaa ctgaacatg aaagtagtc 
      aaccctgtc ctgtccaat tttttcatg aaagtggtc 
      gaagacacc gtcaatatg cgcgaacgc aacaacatc 
      taccgcaaa gatttcatg catctcctg ctccagctg 
      aaaaatcga ggggaactt gttgacgac gacagcatc 
      ctacaaaac aataccgat gccgaaaat aaggaagtg 
      acgctgact ttgaacgag ctcgcagcc caagctttc 
      gtcttcttc ctggccggt tacgagacc tcctccacc 
      actatgacg ttttgcttg tacgaatta gcgtcgaat 
      cctgaaatt caggacaaa ttgcgcgct gaaataaac 
      gaagttttg caaaagcac ggcaaattg acgtatgac 
      gcaattatg gaaatgcgt tacatggac aaagtcgtg 
      aatgaaact ctacgcaag tatccgccc cttccgggc 
      ctgaaccgc gtctgcaac aaggaatac aaagtcccc 
      ggaacagat tttgtgatt gaaaagggg acgaaaatc 
      tggattccg gtgctgggt ttgcaccgg gacccggag 
      tatttccca aatccggag aaattcgac ccggagagg 
      ttcacggag gagaacaag aggcagaga cacccttat 
      acgtatcta cccttcggc gaaggacct aggatttgc 
      attggtctt aggttcggg atgatgcaa actaaagtc 
      gggcttagc gtccttttg aagaattac aagttttcc 
      attaattcc aaaaccaaa ctgccgctg caacttgac 
      cccaaaagc ttcatcatg agcacagaa ggcgggatt 
      tggcttgat tatacgcgc gtttaaact gtaggtaat 
      tcaataaaa attgtaaaa taataacac aaaatgatg 
      tttcctgat ttatttcga cgggcacgt gaccagaat 
      tttcaagat gtattttca ttctgcacg tgtgtggta 
      aaatacaat aaaaaagct tcaatgtct acagaaaat 
      cataaagta aaagttact ctgttcatc aaatactcg 
      caaaaataa tgtggttaa gagggggtt caataaatg 
      tctcaacta attaaataa aatattatt tattaaatg 
      cttaagtag ctaattttg tcacaatct ataaataac 
      aatatcaca acaaaaacc gtgtgaaaa tggagtctc 
      gtattaggt ttctataca catcaagtt tcaatcaaa 
      agaatccac taacataac aaaaaagct attccgagt 
      ggatttttt attgaaact cggtttttc acgacaaaa 
      aatggcatg ctcttatta aacaactat aaaatctta 
      agctgttat aacatttgt atatctttt aattaatgt 
      aatgcattt tcgattcat ttgagttag tttaaataa 
      atcgattat atacatacg aagtttgcc agcaaatac 
      acaccgata atgtctgtt taagtaaaa atatgataa 
      aattccatt ttaatgttt caagcatta ttaccacaa 
      aattgggta attgtttgt gtatgtgaa ttaattcaa 
      ttaaaccct aaatgtgtg taaagcacc agttcaaaa 
      gaaattaaa acataaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY