Status
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Gene information
General information
TC001983 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 169255..171783
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001983
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001983 RA
      MAGPTTINN RGPCFWCIK ALKWTPVLF IVCIIAWSY 
      YAYVVQLCL AIVESTPKQ VIFLVFYHI FFIMLCWSY 
      WQTIFTEIG RVPIKYKID DADFHTLTV TESAEAQRQ 
      ILENVSRHL PNTNVNVQH FPRFCDKCR VIKPDRAHH 
      CSVCGECVL KMDHHCPWI NNCVCFTNY KFFLLFLGY 
      ALFYCVYVA LTSLPYFIE FWRGTLEGK GNGRFHILF 
      LFFVAIMFG VSLVSLFCY HCYLVSENR TTLEAFRPP 
      IFRSGPDKR GFNLGRYNN FQEVFGDNP RTWFIPIKT 
      SLGDGIEFP LNSRHQANS YQSMENTQN SSREVDSET 
      SKLNHGHIV T
    • >TC001983 RB
      MAGPTTINN RGPCFWCIK ALKWTPVLF IVCIIAWSY 
      YAYVVQLCL AIVESTPKQ VIFLVFYHI FFIMLCWSY 
      WQTIFTEIG RVPIKYKID DADFHTLTV TESAEAQRQ 
      ILENVSRHL PNTNVNVQH FPRFCDKCR VIKPDRAHH 
      CSVCGECVL KMDHHCPWI NNCVCFTNY KFFLLFLGY 
      ALFYCVYVA LTSLPYFIE FWRGTLEGK GNGRFHILF 
      LFFVAIMFG VSLVSLFCY HCYLVSENR TTLEAFRPP 
      IFRSGPDKR GFNLGRYNN FQEVFGDNP RTWFIPIKT 
      SLGDGIEFP LNSRHQANS YQSMENTQN RLDV
    CDS sequence
    • >TC001983 RA
      atggcaggc cccacaaca attaacaac aggggacca 
      tgcttctgg tgcatcaag gccctcaag tggacacca 
      gtcttattc atagtttgc ataatcgcg tggtcctac 
      tatgcctac gttgtccag ctttgtcta gcgattgtc 
      gaatcaacc ccaaaacaa gtgattttc ctagtattc 
      taccatata ttttttata atgctgtgc tggtcttac 
      tggcagacg attttcacg gaaatcggg cgcgtgccg 
      atcaaatac aaaatcgac gacgctgac tttcacaca 
      ctcacagtg accgaatcg gcggaagcg caacgccaa 
      attttggaa aatgtctcg cggcatttg ccgaacacc 
      aacgtaaac gtccaacat tttccccgc ttttgcgac 
      aagtgtagg gtcattaag cccgacagg gctcaccat 
      tgctccgtt tgtggcgag tgcgtcctc aaaatggac 
      catcactgt ccctggatc aacaattgt gtttgtttc 
      acaaattat aagtttttc ttgttgttc ttgggctac 
      gcgctgttc tattgcgtg tacgtcgcc ttgacgtct 
      ctcccctat ttcatcgag ttttggagg ggcacgctg 
      gaggggaaa gggaacggg cggtttcac attctgttt 
      ctgttcttc gtggcgatc atgtttgga gtaagtctg 
      gtctcgttg ttttgttac cactgttac ctggttagt 
      gagaatcga acaactctc gaagcattt cgtccgccg 
      attttccga agcggtccg gataagagg ggttttaat 
      ctgggacgg tataacaat ttccaggag gttttcggt 
      gataatcct cggacttgg tttattccg attaagact 
      agtttgggt gacggtatt gaattcccg ctaaactca 
      cgtcatcag gcgaattct tatcagtcc atggagaac 
      acacagaac agcagtcga gaggttgat tcagagaca 
      agcaagctc aaccacgga cacatagtt acataa
    • >TC001983 RB
      atggcaggc cccacaaca attaacaac aggggacca 
      tgcttctgg tgcatcaag gccctcaag tggacacca 
      gtcttattc atagtttgc ataatcgcg tggtcctac 
      tatgcctac gttgtccag ctttgtcta gcgattgtc 
      gaatcaacc ccaaaacaa gtgattttc ctagtattc 
      taccatata ttttttata atgctgtgc tggtcttac 
      tggcagacg attttcacg gaaatcggg cgcgtgccg 
      atcaaatac aaaatcgac gacgctgac tttcacaca 
      ctcacagtg accgaatcg gcggaagcg caacgccaa 
      attttggaa aatgtctcg cggcatttg ccgaacacc 
      aacgtaaac gtccaacat tttccccgc ttttgcgac 
      aagtgtagg gtcattaag cccgacagg gctcaccat 
      tgctccgtt tgtggcgag tgcgtcctc aaaatggac 
      catcactgt ccctggatc aacaattgt gtttgtttc 
      acaaattat aagtttttc ttgttgttc ttgggctac 
      gcgctgttc tattgcgtg tacgtcgcc ttgacgtct 
      ctcccctat ttcatcgag ttttggagg ggcacgctg 
      gaggggaaa gggaacggg cggtttcac attctgttt 
      ctgttcttc gtggcgatc atgtttgga gtaagtctg 
      gtctcgttg ttttgttac cactgttac ctggttagt 
      gagaatcga acaactctc gaagcattt cgtccgccg 
      attttccga agcggtccg gataagagg ggttttaat 
      ctgggacgg tataacaat ttccaggag gttttcggt 
      gataatcct cggacttgg tttattccg attaagact 
      agtttgggt gacggtatt gaattcccg ctaaactca 
      cgtcatcag gcgaattct tatcagtcc atggagaac 
      acacagaac aggttagac gtataa
    mRNA sequence
    • >TC001983 RA
      tggcacctc cattttggt gacattttc ctaaatagc 
      aaataaaaa attgttgat gtgtgcgtt aggtgtgtg 
      gtttaatta atgaaattc caaagaact atgtgaatg 
      aagtgagtt gggaatgac tcgttagtt gcctagagc 
      cccgcccct ttgtgatgc ctggaggtg tccgtgcga 
      tctcattag cctttgaaa tgcctcaac gtaattgaa 
      acaaatcaa aactaggtt tagggaaaa tggcaggcc 
      ccacaacaa ttaacaaca ggggaccat gcttctggt 
      gcatcaagg ccctcaagt ggacaccag tcttattca 
      tagtttgca taatcgcgt ggtcctact atgcctacg 
      ttgtccagc tttgtctag cgattgtcg aatcaaccc 
      caaaacaag tgattttcc tagtattct accatatat 
      tttttataa tgctgtgct ggtcttact ggcagacga 
      ttttcacgg aaatcgggc gcgtgccga tcaaataca 
      aaatcgacg acgctgact ttcacacac tcacagtga 
      ccgaatcgg cggaagcgc aacgccaaa ttttggaaa 
      atgtctcgc ggcatttgc cgaacacca acgtaaacg 
      tccaacatt ttccccgct tttgcgaca agtgtaggg 
      tcattaagc ccgacaggg ctcaccatt gctccgttt 
      gtggcgagt gcgtcctca aaatggacc atcactgtc 
      cctggatca acaattgtg tttgtttca caaattata 
      agtttttct tgttgttct tgggctacg cgctgttct 
      attgcgtgt acgtcgcct tgacgtctc tcccctatt 
      tcatcgagt tttggaggg gcacgctgg aggggaaag 
      ggaacgggc ggtttcaca ttctgtttc tgttcttcg 
      tggcgatca tgtttggag taagtctgg tctcgttgt 
      tttgttacc actgttacc tggttagtg agaatcgaa 
      caactctcg aagcatttc gtccgccga ttttccgaa 
      gcggtccgg ataagaggg gttttaatc tgggacggt 
      ataacaatt tccaggagg ttttcggtg ataatcctc 
      ggacttggt ttattccga ttaagacta gtttgggtg 
      acggtattg aattcccgc taaactcac gtcatcagg 
      cgaattctt atcagtcca tggagaaca cacagaaca 
      gcagtcgag aggttgatt cagagacaa gcaagctca 
      accacggac acatagtta cataattaa ttcgacaga 
      aattgtact tatatgttg ttaacaacc atttagttt 
      tttcttttg taataaacg tttttataa tttagtttc 
      gtttttatt attattggt aacagaaca tttcattaa 
      ccattcccg gagcctaga ttagttgca gctttgatc 
      cgtttgtta cttagattt aaatgtaat tgttgttct 
      gttgtttag tttgggcga cggggttac gttccctca 
      aaggcatat ggacgaaga cagagaagg gcttcttaa 
      tcataactt agatgaaga agcaaacta ttatgcttc 
      atagtcaat gttaagaaa acttctcga ttttttata 
      atatgcata gttccacgt aaattgtgc ttacagtta 
      ctttaacgc caaagcact aacattgtt cctgtgtta 
      tgttttaac gatgttttt cgttcaaaa tatagtttg 
      actggttta tcccagtta tttcgtttt ttactcaat 
      aaactaatt ttctaaat
    • >TC001983 RB
      tggcacctc cattttggt gacattttc ctaaatagc 
      aaataaaaa attgttgat gtgtgcgtt aggtgtgtg 
      gtttaatta atgaaattc caaagaact atgtgaatg 
      aagtgagtt gggaatgac tcgttagtt gcctagagc 
      cccgcccct ttgtgatgc ctggaggtg tccgtgcga 
      tctcattag cctttgaaa tgcctcaac gtaattgaa 
      acaaatcaa aactaggtt tagggaaaa tggcaggcc 
      ccacaacaa ttaacaaca ggggaccat gcttctggt 
      gcatcaagg ccctcaagt ggacaccag tcttattca 
      tagtttgca taatcgcgt ggtcctact atgcctacg 
      ttgtccagc tttgtctag cgattgtcg aatcaaccc 
      caaaacaag tgattttcc tagtattct accatatat 
      tttttataa tgctgtgct ggtcttact ggcagacga 
      ttttcacgg aaatcgggc gcgtgccga tcaaataca 
      aaatcgacg acgctgact ttcacacac tcacagtga 
      ccgaatcgg cggaagcgc aacgccaaa ttttggaaa 
      atgtctcgc ggcatttgc cgaacacca acgtaaacg 
      tccaacatt ttccccgct tttgcgaca agtgtaggg 
      tcattaagc ccgacaggg ctcaccatt gctccgttt 
      gtggcgagt gcgtcctca aaatggacc atcactgtc 
      cctggatca acaattgtg tttgtttca caaattata 
      agtttttct tgttgttct tgggctacg cgctgttct 
      attgcgtgt acgtcgcct tgacgtctc tcccctatt 
      tcatcgagt tttggaggg gcacgctgg aggggaaag 
      ggaacgggc ggtttcaca ttctgtttc tgttcttcg 
      tggcgatca tgtttggag taagtctgg tctcgttgt 
      tttgttacc actgttacc tggttagtg agaatcgaa 
      caactctcg aagcatttc gtccgccga ttttccgaa 
      gcggtccgg ataagaggg gttttaatc tgggacggt 
      ataacaatt tccaggagg ttttcggtg ataatcctc 
      ggacttggt ttattccga ttaagacta gtttgggtg 
      acggtattg aattcccgc taaactcac gtcatcagg 
      cgaattctt atcagtcca tggagaaca cacagaaca 
      ggttagacg tataaatat tagctaacg ctagctaaa 
      gcaagtgtt attagtttg atgcctgac acctgattt 
      ttttcagtt aattaattt gttgttatt ttttactaa 
      ctccatcac tcatgtatt ttttttaat aaattcagt 
      ctgttttac ccagtttta ttgattaca cacaaaaca 
      ttaaattac gaggtgttg gtacgacta acctttcgc 
      taattctgg ctaattgta gtttgtgca tgaggcttt 
      ttgtgatag cttttgttt taacatgaa aattgaatt 
      ttgttaaac agcagtcga gaggttgat tcagagaca 
      agcaagctc aaccacgga cacatagtt acataatta 
      attcgacag aaattgtac ttatatgtt gttaacaac 
      catttagtt ttttctttt gtaataaac gtttttata 
      atttagttt cgtttttat tattattgg taacagaac 
      atttcatta accattccc ggagcctag attagttgc 
      agctttgat ccgtttgtt acttagatt taaatgtaa 
      ttgttgttc tgttgttta gtttgggcg acggggtta 
      cgttccctc aaaggcata tggacgaag acagagaag 
      ggcttctta atcataact tagatgaag aagcaaact 
      attatgctt catagtcaa tgttaagaa aacttctcg 
      attttttat aatatgcat agttccacg taaattgtg 
      cttacagtt actttaacg ccaaagcac taacattgt 
      tcctgtgtt atgttttaa cgatgtttt tcgttcaaa 
      atatagttt gactggttt atcccagtt atttcgttt 
      tttactcaa taaactaat tttctaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY