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Gene information
General information
TC033554
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009306
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 151907..156441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033554
    iBeetle screen iB_08963

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 4

    mature egg potentially deposition irregular - Number of animals showing the phenotype: 1

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: just 1
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033554 RA
      MDNRPKSIF RDLYNLPYK NNFFKLSTN AKDDGDLIQ 
      RLGLLKKLE IHQGCVNTI CWNSTGEYI LSGSDDQHL 
      IITNGHTYK VETNYCTSH HANIFSAKF LPCSSDKQI 
      VSCSGDGII LHTDLENTK NTYYNQFSC HAGTTYEDG 
      TVRWFDLRT KTSCRKPRC KEDILIKCQ RAVTALAIS 
      PTAPHQLAV GCADSTVRI YDRRCLSTQ VEDPLKARP 
      FCSFIAPNL EDRRYRITS LSYSHDGQD MLENQLAEL 
      KKPIKASPW EKVKMFAGQ RERVNNSPP HMRRLRLRG 
      DWSDTGPDA RPERDTTTT VSIGQARPQ LHATLMQRM 
      TDVLSQMLN DPMTRAALS AGGENETSQ RNILGERQS 
      NSSAQEENA EQPVEQSQN EAEGEQSVH TEAEEVATT 
      SR
    CDS sequence
    • >TC033554 RA
      atggataat agacctaaa tcgatattt cgggactta 
      tacaacctt ccttataaa aacaatttt ttcaaatta 
      tcaactaat gctaaagac gacggtgat cttatacag 
      agactaggt ttgctcaag aagttagaa atccatcaa 
      ggctgtgtc aacaccatt tgttggaac agcacagga 
      gagtatatt ttgtcgggt tcagacgac cagcattta 
      atcataaca aatgggcac acatacaaa gttgaaacc 
      aattattgc accagccac catgcgaac atcttcagt 
      gctaagttc ttgccatgc tcttctgat aaacagatc 
      gtttcgtgt tcaggcgat ggaatcatc ttacacaca 
      gatctcgaa aacaccaaa aatacttac tacaaccag 
      ttcagttgc catgcagga accacttat gaggatggc 
      acagtgcgt tggtttgat ttgaggaca aagacaagt 
      tgccgaaaa ccgcgttgt aaagaagat attttaatt 
      aagtgtcag agagctgtt acagctctt gcaataagt 
      ccaactgca cctcatcag cttgcagtt ggatgcgct 
      gattcaaca gtgcgtatt tatgacaga cgttgtctt 
      agtactcaa gtcgaggat cctttaaag gctcgacca 
      ttctgtagt ttcatcgcc ccaaatctg gaagaccga 
      agatacaga ataacttca ctcagctac agtcatgac 
      ggtcaagat atgctagag aatcagcta gctgaatta 
      aaaaaaccc attaaagcg tctccttgg gagaaagtt 
      aaaatgttc gcaggccaa cgagaacgt gtcaataat 
      tcgccacct catatgcga aggttacgt ttaagagga 
      gattggtct gatactgga ccagatgca cgtccagaa 
      agagacaca acgacaact gtcagtatt ggtcaagcg 
      agaccacaa ttacatgcc actttgatg cagcgtatg 
      accgatgtt ctctcacaa atgttgaat gatccaatg 
      acaagagcg gctttgagt gcaggagga gagaatgaa 
      acgtcgcaa agaaatatt ttaggtgaa agacaaagt 
      aattcgtct gctcaggaa gagaacgca gaacagccg 
      gtggaacaa agtcagaat gaagcggaa ggagagcaa 
      agtgttcac acagaggca gaagaagtt gccacaaca 
      agcaggtaa
    mRNA sequence
    • >TC033554 RA
      tatttttag tccactttg gtgatcttg gggcaagtt 
      gctgtgata atttaaata acttaatta tggataata 
      gacctaaat cgatatttc gggacttat acaaccttc 
      cttataaaa acaattttt tcaaattat caactaatg 
      ctaaagacg acggtgatc ttatacaga gactaggtt 
      tgctcaaga agttagaaa tccatcaag gctgtgtca 
      acaccattt gttggaaca gcacaggag agtatattt 
      tgtcgggtt cagacgacc agcatttaa tcataacaa 
      atgggcaca catacaaag ttgaaacca attattgca 
      ccagccacc atgcgaaca tcttcagtg ctaagttct 
      tgccatgct cttctgata aacagatcg tttcgtgtt 
      caggcgatg gaatcatct tacacacag atctcgaaa 
      acaccaaaa atacttact acaaccagt tcagttgcc 
      atgcaggaa ccacttatg aggatggca cagtgcgtt 
      ggtttgatt tgaggacaa agacaagtt gccgaaaac 
      cgcgttgta aagaagata ttttaatta agtgtcaga 
      gagctgtta cagctcttg caataagtc caactgcac 
      ctcatcagc ttgcagttg gatgcgctg attcaacag 
      tgcgtattt atgacagac gttgtctta gtactcaag 
      tcgaggatc ctttaaagg ctcgaccat tctgtagtt 
      tcatcgccc caaatctgg aagaccgaa gatacagaa 
      taacttcac tcagctaca gtcatgacg gtcaagata 
      tgctagaga atcagctag ctgaattaa aaaaaccca 
      ttaaagcgt ctccttggg agaaagtta aaatgttcg 
      caggccaac gagaacgtg tcaataatt cgccacctc 
      atatgcgaa ggttacgtt taagaggag attggtctg 
      atactggac cagatgcac gtccagaaa gagacacaa 
      cgacaactg tcagtattg gtcaagcga gaccacaat 
      tacatgcca ctttgatgc agcgtatga ccgatgttc 
      tctcacaaa tgttgaatg atccaatga caagagcgg 
      ctttgagtg caggaggag agaatgaaa cgtcgcaaa 
      gaaatattt taggtgaaa gacaaagta attcgtctg 
      ctcaggaag agaacgcag aacagccgg tggaacaaa 
      gtcagaatg aagcggaag gagagcaaa gtgttcaca 
      cagaggcag aagaagttg ccacaacaa gcaggtaac 
      atgatgaga acaatagaa aaaaaatga aaccagtca 
      caatttatt catt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY