Status
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Gene information
General information
TC001905 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451796.1: 63205..65059
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001905
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_08962

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Adult morphology
      abdominal odoriferous glands content split - Number of animals showing the phenotype: 6 (13 dpi)
      Days post injection



      abdominal odoriferous glands partiallycontent opaque - Number of animals showing the phenotype: 6 (13 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001905 RA
        MEDEKGNKF TAQGGSSPV PVAGGGKAG LSRPSASNR 
        GASSVGNQA EMLLATGEK EKDQAEGAG RASGSMVDT 
        PKTGPLHSR LNLTSRLQR CSSLSEGST KKRKYVDSS 
        PSPKTAYNK KESAVAFSA IEAIGNIVK IAEKLNNTI 
        KASYQPKKE IVHMVESIN RNTKVFANE SIKAWLEEV 
        RWEPVERIT YDADAQTDP PQEAEDTKT LRAELEAVK 
        QQAAKQAKE IEILKRTLQ DLEREREQK QGVDATAQS 
        TDDDKESIR REILTQMDR PGTYAEVER VLNLEWPQD 
        VYQATKVIK GSPLAASRD WDLGIFLNE EDGGLDTTA 
        MGRLFKERY PELVEEEEE QDDISYIVQ TTKFKSKVN 
        TKYVWKIRI EKSMTNWED KALNAEILK KLRDIADAM 
        QVEGRVKVA IPIPPRASL DQLRKLAEI AFRNKGREL 
        AIHVPQNFT VNGIPTANP NPKQTKRRG VEKQTGALI 
        VRKPGCEYI DLLKKVKKT LGNSENLKI YKALKTNKG 
        DLLIVTENN EEALANARK TIVEEDNAI SVTISRPRG 
        EGMELHIGN IEADITAEQ ISEAH
      CDS sequence
      • >TC001905 RA
        atggaggac gaaaaagga aataagttt actgcacag 
        gggggaagt tccccagtg ccggtcgcc ggaggtggt 
        aaagcgggc ctctcccga ccgtcagca agcaaccgt 
        ggggcatct tcggtgggc aaccaggcc gagatgctt 
        cttgctact ggagagaaa gagaaagac caagcggag 
        ggggctgga agagcttct ggtagtatg gtggataca 
        ccgaaaaca ggtcctcta cacagcaga ttaaatctc 
        actagcaga ctacagagg tgtagttcc ctatcggag 
        ggcagtaca aaaaagagg aagtacgtc gactcatcg 
        ccaagtcca aagactgct tacaataaa aaggaaagt 
        gcagtagcc ttctcggcg attgaggca ataggaaat 
        atcgtcaaa atagcggag aagctgaat aacaccatc 
        aaagcatcg taccagccc aagaaagag atcgtacat 
        atggtggag agcatcaat aggaacact aaagttttc 
        gcaaatgag tccataaag gcctggttg gaggaagtg 
        agatgggag cctgtagaa aggatcacc tacgatgcg 
        gatgcgcag acggacccg ccacaggag gctgaggat 
        accaagact ctcagggcg gagctggag gcggttaaa 
        cagcaagca gctaagcag gcaaaggaa attgagatc 
        ctcaaacgg acgctccaa gatcttgaa agggaaagg 
        gagcagaaa caaggggta gacgccacc gcccaaagc 
        accgatgac gacaaagaa tccatcagg agggaaatc 
        ctgacacag atggaccgt cccggcaca tatgcggag 
        gttgagaga gtactaaac ctagaatgg ccgcaagac 
        gtctatcaa gctacgaag gtaatcaag ggatcaccg 
        cttgccgca tctagagac tgggacctg ggaattttc 
        ctcaacgaa gaggatggg ggtttagac accacagct 
        atggggagg ctgttcaaa gagagatac ccagaattg 
        gtcgaggaa gaggaagaa caagacgac atctcttac 
        attgtacag acaacgaag ttcaagtca aaggtaaac 
        acaaaatac gtatggaag attcggatc gagaagtcg 
        atgacaaat tgggaagat aaagcatta aatgcagaa 
        atcttaaag aaactgagg gacattgct gatgcaatg 
        caggtcgaa ggtagggta aaagttgcc atcccaatc 
        cctccgagg gcatccctc gatcagcta agaaaactg 
        gcggaaatc gcttttagg aacaaggga agagaactg 
        gctatacat gttccccaa aatttcacc gtaaacggg 
        atacccacg gcaaatcca aatccaaaa caaacaaag 
        agaagaggt gtggaaaaa caaactggg gcactcata 
        gtacggaag cctgggtgc gagtatatt gaccttctc 
        aagaaagtc aaaaagaca ttgggtaac tcggagaat 
        cttaaaata tataaggca ctcaagacc aataagggc 
        gaccttctg atcgttacc gagaataac gaggaagca 
        ttggcaaac gccaggaag acaatagtg gaagaggac 
        aacgccata tcggtgaca atcagcaga cctagggga 
        gagggaatg gagctacat atcggaaac atcgaagcc 
        gatatcaca gcggaacaa atctcggaa gctcactaa
      mRNA sequence
      • >TC001905 RA
        ggattcgac aaaggacaa gacgacaac acgcctgcc 
        aggaggcat aaaatgaaa ttctacgag cttggttta 
        tatgatgga ggacgaaaa aggaaataa gtttactgc 
        acagggggg aagttcccc agtgccggt cgccggagg 
        tggtaaagc gggcctctc ccgaccgtc agcaagcaa 
        ccgtggggc atcttcggt gggcaacca ggccgagat 
        gcttcttgc tactggaga gaaagagaa agaccaagc 
        ggagggggc tggaagagc ttctggtag tatggtgga 
        tacaccgaa aacaggtcc tctacacag cagattaaa 
        tctcactag cagactaca gaggtgtag ttccctatc 
        ggagggcag tacaaaaaa gaggaagta cgtcgactc 
        atcgccaag tccaaagac tgcttacaa taaaaagga 
        aagtgcagt agccttctc ggcgattga ggcaatagg 
        aaatatcgt caaaatagc ggagaagct gaataacac 
        catcaaagc atcgtacca gcccaagaa agagatcgt 
        acatatggt ggagagcat caataggaa cactaaagt 
        tttcgcaaa tgagtccat aaaggcctg gttggagga 
        agtgagatg ggagcctgt agaaaggat cacctacga 
        tgcggatgc gcagacgga cccgccaca ggaggctga 
        ggataccaa gactctcag ggcggagct ggaggcggt 
        taaacagca agcagctaa gcaggcaaa ggaaattga 
        gatcctcaa acggacgct ccaagatct tgaaaggga 
        aagggagca gaaacaagg ggtagacgc caccgccca 
        aagcaccga tgacgacaa agaatccat caggaggga 
        aatcctgac acagatgga ccgtcccgg cacatatgc 
        ggaggttga gagagtact aaacctaga atggccgca 
        agacgtcta tcaagctac gaaggtaat caagggatc 
        accgcttgc cgcatctag agactggga cctgggaat 
        tttcctcaa cgaagagga tgggggttt agacaccac 
        agctatggg gaggctgtt caaagagag atacccaga 
        attggtcga ggaagagga agaacaaga cgacatctc 
        ttacattgt acagacaac gaagttcaa gtcaaaggt 
        aaacacaaa atacgtatg gaagattcg gatcgagaa 
        gtcgatgac aaattggga agataaagc attaaatgc 
        agaaatctt aaagaaact gagggacat tgctgatgc 
        aatgcaggt cgaaggtag ggtaaaagt tgccatccc 
        aatccctcc gagggcatc cctcgatca gctaagaaa 
        actggcgga aatcgcttt taggaacaa gggaagaga 
        actggctat acatgttcc ccaaaattt caccgtaaa 
        cgggatacc cacggcaaa tccaaatcc aaaacaaac 
        aaagagaag aggtgtgga aaaacaaac tggggcact 
        catagtacg gaagcctgg gtgcgagta tattgacct 
        tctcaagaa agtcaaaaa gacattggg taactcgga 
        gaatcttaa aatatataa ggcactcaa gaccaataa 
        gggcgacct tctgatcgt taccgagaa taacgagga 
        agcattggc aaacgccag gaagacaat agtggaaga 
        ggacaacgc catatcggt gacaatcag cagacctag 
        gggagaggg aatggagct acatatcgg aaacatcga 
        agccgatat cacagcgga acaaatctc ggaagctca 
        ctaagaagg gaccagcgg aatgcaggt cgcgacgat 
        acaggtcaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY