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Gene information
General information
TC008549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451492.1: 159019..166378
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008549
    iBeetle screen iB_08961

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008549 RA
      MSQDRCFAN PFDDYSSEA ACCVIENGT IKCDCKLSC 
DLEKKWHDT LAQFKYLDY NDYSVYTGT SGIALLKLK 
KDPESRQNL QEALQLLSL HRLKGRRHT FLCGDAGPL 
ALGAIINYK LEDKAYLDC IEKLKALSE DVFDPSSDL 
PNEYLYGRA GFLFAVLYV NKHVSPVPF EESFIRKII 
ETIILCGKN EAKAGKFKC PLMYQWHES YYLGAAHGV 
SGILYLLLQ AKEYLTENE LQTIIKPTV DYLATLRFP 
SCNFPSSLG RDNDKYVQW CHGAPGFLY LFTTAYKVF 
GDCKYLQLA LDCGDVIWS RGILRKGYS ICHGVSGNA 
YCFLELYQT TEDEKHLYR AVRFAEWCF AYNKNHVEH 
PPDRPLSLF EGLSGPMYL FLDIQKPAE AKFPGYTL
    CDS sequence
    • >TC008549 RA
      atgtcgcaa gatcgatgt ttcgccaat ccatttgat 
gattattcg tcagaagca gcatgttgt gtaatcgaa 
aatggaacg attaaatgc gattgtaaa ttatcgtgc 
gatttggag aaaaagtgg cacgatacg ttggcacag 
tttaaatat cttgattat aatgattat tctgtgtat 
acaggaact tccggtatt gctttactg aaactcaaa 
aaggatcct gaaagtaga caaaacttg caggaggca 
ttacaactg ttgtcatta catagacta aaaggaaga 
agacatact tttttgtgt ggagatgcc ggacccctt 
gctctgggt gctattatc aattataaa ttggaagat 
aaggcttac ttggactgc atagaaaag cttaaagca 
ctatcagag gacgttttt gacccaagc tcagattta 
ccaaacgag tacctttat ggtcgagct ggttttctt 
tttgcagtt ttatacgtc aataaacat gtttcacct 
gtaccattt gaagaaagt tttatcaga aaaataatt 
gaaacaata atactttgt ggaaaaaat gaagcaaag 
gctggaaaa tttaaatgt ccattgatg tatcagtgg 
catgagagt tactatctg ggggcagct catggtgtg 
tctggaatt ctctatttg ctccttcaa gccaaggag 
tatttaacc gaaaatgaa ctacaaaca ataattaaa 
ccgactgtt gattacttg gccactttg agatttccg 
agttgtaat tttccctca tctctggga cgagataac 
gacaaatat gtccagtgg tgtcatggg gcgcccggc 
tttttgtat ctcttcact acagcttac aaggttttc 
ggtgactgc aaatatcta caactggct ttggattgt 
ggcgacgta atttggtct cgaggcata ttgagaaaa 
gggtacagt atctgccat ggtgtctca ggcaatgct 
tattgtttc ttggagttg taccaaacc actgaagat 
gaaaaacat ttataccgg gctgttcga ttcgcagaa 
tggtgcttc gcttacaat aaaaaccac gtggagcac 
cctcctgat cgccccctt tccttattt gaggggcta 
tcaggacct atgtacttg tttttggat attcaaaaa 
ccggccgaa gccaaattt cctggatac actttgtaa
    mRNA sequence
    • >TC008549 RA
      cttaaatta ttagttaaa tagttacaa ttgtttgcc 
tcctggaat taaaacaaa agtccctaa cttgccgca 
cctgtcaca tttgtaaac aaacatcaa aataataac 
aaatcaagc aaaattaat tttctcaag tgtttttca 
gtgaaaatg tcgcaagat cgatgtttc gccaatcca 
tttgatgat tattcgtca gaagcagca tgttgtgta 
atcgaaaat ggaacgatt aaatgcgat tgtaaatta 
tcgtgcgat ttggagaaa aagtggcac gatacgttg 
gcacagttt aaatatctt gattataat gattattct 
gtgtataca ggaacttcc ggtattgct ttactgaaa 
ctcaaaaag gatcctgaa agtagacaa aacttgcag 
gaggcatta caactgttg tcattacat agactaaaa 
ggaagaaga catactttt ttgtgtgga gatgccgga 
ccccttgct ctgggtgct attatcaat tataaattg 
gaagataag gcttacttg gactgcata gaaaagctt 
aaagcacta tcagaggac gtttttgac ccaagctca 
gatttacca aacgagtac ctttatggt cgagctggt 
tttcttttt gcagtttta tacgtcaat aaacatgtt 
tcacctgta ccatttgaa gaaagtttt atcagaaaa 
ataattgaa acaataata ctttgtgga aaaaatgaa 
gcaaaggct ggaaaattt aaatgtcca ttgatgtat 
cagtggcat gagagttac tatctgggg gcagctcat 
ggtgtgtct ggaattctc tatttgctc cttcaagcc 
aaggagtat ttaaccgaa aatgaacta caaacaata 
attaaaccg actgttgat tacttggcc actttgaga 
tttccgagt tgtaatttt ccctcatct ctgggacga 
gataacgac aaatatgtc cagtggtgt catggggcg 
cccggcttt ttgtatctc ttcactaca gcttacaag 
gttttcggt gactgcaaa tatctacaa ctggctttg 
gattgtggc gacgtaatt tggtctcga ggcatattg 
agaaaaggg tacagtatc tgccatggt gtctcaggc 
aatgcttat tgtttcttg gagttgtac caaaccact 
gaagatgaa aaacattta taccgggct gttcgattc 
gcagaatgg tgcttcgct tacaataaa aaccacgtg 
gagcaccct cctgatcgc cccctttcc ttatttgag 
gggctatca ggacctatg tacttgttt ttggatatt 
caaaaaccg gccgaagcc aaatttcct ggatacact 
ttgtaagtg tgcaatcaa aatacgaat aaataaaat 
atccacttt att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY