Status
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Gene information
General information
TC031725
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001824,TC001823
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451780.1: 36732..47442
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031725
      iBeetle screen iB_08959

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031725 RA
        MDKFLVRTP IDLQKKMNS TDTETPIMA GKERGENSE 
        NKTADSQSD DVDMDIDEE PETVQEELV ETTRIRESI 
        ITNLVRRGN LYNDATQNP TIIDRVALK VPPFWAEDP 
        SLWFLQLEG QFQLNRVTL DATKYFYVI TNLETRYLM 
        EIKDVITNI PETNNQEQK TRRLLEREE MGDRTPSQF 
        LRHLRDLAG ANVHESFLR TLWTGRLPR NMQAILATQ 
        SKCTLDEVA VSADKIHET IPEARVFAI NNNANASSA 
        TTGTHGNFD ATISALSQQ VQDLSRQLA ALQTSQSGH 
        ASRFGTRAP SRYRSRSRS KPRDGKCWF HHRFGDKAR 
        KCEAPCSYQ KKKRHKQSV VAAYDCARN TCRLFIRNR 
        DDGVPFLID TSADVSVFP KKLLRGSPP ASDVVLFAV 
        NGTRFPTYG LRTLQLNLG LRRVFKWQF IIADVSYAI 
        IGADFIHHY KLLVDLRNR RLIDSLTQV CAECEIRAV 
        DVPTVKTLC IAGASEYHR LLLEFPAIT KPPNIHQER 
        RHNTVHFIR TTDGPPVSC RPRRLAPDR YKAAKKEFD 
        AMVRDGIAR PSDGNWSSP LHLVPKKDG NLRPCGDYR 
        RLNARTIPD RYPVPHIED FSHALHGKK VFSTIDLVR 
        AYHQIPVNP QDVPKTAIT TPFGLFEYT QLSFGLRNA 
        SQTFQRFIY EVTHELDFC FAYIDDVLV ASENSQQHL 
        RKLFTRFLD YGVVINSDK CKFGQSEVT FLGYHISDK 
        GTQPPTDRV QAILDMKKP STLKELQRF LGMVNFYRR 
        FIPQAARIQ APLTQLQGG PKAKQKETI KWCPETENA 
        FEATKRSLA NAALLAHPS PEAPLSICM NQNPEKASP 
        RQFRYLDFI SQFTTDIQH VSGASNIPA DTLSRIETV 
        SGVQIHFFK LPELAKAQE DDAELKHLF SSQNSCSLN 
        KLYFPEHAC SIVCDTSTG TIRPYLPEA YRKQVFYNV 
        HNLSHPGVN ATTKMVKQK YMWPGIDKN CRTWARQCI 
        SCQRSKITR HTKSNFGSF PTPTNRFRH LHLDLVGPL 
        PPSHGYKYV LTCIDRFTR WPEAIPIKD ISAETVAQA 
        LLHTWIARF GIPELITTD RGTQFDSEL FRNMSKLFG 
        ICHCKTTAY HPQANGMIE RLHQQLKTS IKCHNTENW 
        FDCLPLVLL GIRSAVKEN IKSSAAEMV YGEPLSLPG 
        EFFHSPQEE LTSSVLDRL RRKVQDFVA VAPQRHGLP 
        KPSCHWS
      CDS sequence
      • >TC031725 RA
        atggacaag tttttagtc agaactcca attgattta 
        caaaagaag atgaattca acagataca gaaacaccc 
        atcatggct ggaaaggaa cggggagaa aattcagaa 
        aataaaacg gcggacagt cagagtgat gatgtggat 
        atggatatt gatgaggag ccggaaaca gtgcaagaa 
        gaacttgtg gaaacgaca agaataaga gaaagtata 
        atcacaaat ttagtgaga cgtggcaat ctttacaac 
        gacgccacg caaaaccca acgataatc gatcgcgta 
        gcccttaag gtaccacca ttctgggca gaagatcct 
        tccctctgg tttttgcaa ctagaggga caatttcaa 
        ttaaatagg gtgaccctg gacgccaca aaatatttt 
        tacgtaatt acgaatttg gagacaagg tacttaatg 
        gagatcaaa gacgtcatc accaacatc cccgaaacg 
        aacaatcaa gaacagaaa accaggcgc ctcctggaa 
        agagaagag atgggtgat aggacgcca tctcaattc 
        ctacgccat ttgagggac ctagctggc gcaaacgtt 
        catgaatca tttctcaga accttgtgg acagggcgc 
        ctgccgaga aatatgcaa gccatcctt gcgacgcaa 
        tcgaaatgc accttggat gaggtagcg gtgtcggcg 
        gacaaaatc catgaaacc ataccggag gcaagagtt 
        tttgcaatc aacaacaac gcgaacgcc agtagcgcc 
        accaccggc actcatgga aactttgac gcaaccatc 
        agcgccctc tcgcaacaa gtgcaggac ctttcacga 
        caactggca gcactgcag acttcgcag tctggccat 
        gcttcgcgt tttgggact cgggcgcca tctaggtac 
        cggtcgagg agtcgtagc aagccgcgc gatggaaaa 
        tgctggttt caccaccgt tttggcgac aaggcaagg 
        aagtgtgaa gccccatgc tcctaccaa aaaaaaaaa 
        cggcacaag cagtcagta gtggcggca tacgactgc 
        gctcgcaac acttgccgc ctattcatc cgcaatcgt 
        gacgacgga gttccattt cttattgac acaagtgcc 
        gatgtgagt gttttcccc aaaaaactt ctgcgtgga 
        tctccaccg gcgtccgac gtggtgctt tttgcggta 
        aacggtacg cgatttcca acttacgga ctacgaacg 
        ctacagcta aatctgggg ttgcgtcgt gtatttaag 
        tggcagttt attatcgcg gatgtgagc tacgcaata 
        atcggggcg gactttatt caccattac aaacttcta 
        gtagattta cgcaataga cgcttgata gactcatta 
        acccaggtg tgcgcggag tgcgagata cgggcagta 
        gatgtgccg acagttaaa actctatgc attgcgggc 
        gcgagtgag taccaccgt ttactcttg gaattcccc 
        gcaatcacc aagccaccc aatattcat caagagcga 
        aggcacaac accgtccac ttcatccgg acaaccgat 
        ggtccaccc gtttcatgt cgcccccgg cgcttggcg 
        cctgatcgc tacaaggct gcaaaaaag gaattcgat 
        gcaatggtg agagacgga atcgcaaga ccctctgac 
        ggtaactgg tcctctccg ctccacctt gtgcctaaa 
        aaagatggc aatttgagg ccatgcggc gactataga 
        agactaaat gcgcgaact attccggat cgttaccct 
        gtgccccac attgaagat ttttcacac gcactccat 
        ggtaagaaa gtattttca acaatagac ttggttcga 
        gcataccac caaattccg gtcaaccct caagacgtt 
        ccgaaaaca gcgattaca actcctttt ggacttttt 
        gaatacacc caattgtca tttggatta agaaacgcg 
        tcgcaaacg tttcaaaga tttatttat gaagtcaca 
        catgagctc gacttctgt ttcgcatac atagatgac 
        gttctcgtc gcatcagag aactctcaa caacatttg 
        aggaaactg ttcacacgc ttcttagac tacggcgtt 
        gtaattaat tctgataaa tgcaaattc ggtcaatca 
        gaagtaacg tttctggga taccacatt tctgacaag 
        ggcacccaa cctccaacc gatcgtgtc caggccatt 
        ttagacatg aaaaaacct tcgactttg aaagagctg 
        cagcggttt cttggcatg gtaaatttt taccgacgt 
        ttcattcca caggcagcc agaattcaa gcaccgctg 
        acacaactc caagggggg cccaaggcc aaacaaaaa 
        gaaacaatc aagtggtgt ccagaaaca gaaaacgca 
        ttcgaagcc acgaaacga tcgcttgcg aacgctgca 
        ctgctggca catccgtcc ccagaagcc ccattgtca 
        atatgtatg aatcaaaac ccggagaaa gcgtcaccc 
        aggcaattt cgatatcta gacttcata agccaattt 
        acaactgat atccagcac gtttctggc gcctcaaac 
        atcccagca gatactctc tcgcgaatc gaaacggta 
        tcaggtgtt cagatccac ttcttcaaa cttccagaa 
        cttgcaaaa gctcaagaa gacgacgcc gagctaaaa 
        catcttttc tcctctcag aattcatgc tccctaaat 
        aaattatat tttccagaa cacgcctgt tctatagta 
        tgcgacacg tccaccggg acaattcga ccttatctc 
        cccgaggcc taccggaaa caagtgttt tataacgtc 
        cacaacttg tcgcatcca ggtgtgaac gcaacaaca 
        aaaatggtg aagcaaaaa tatatgtgg ccaggcatc 
        gacaaaaat tgtcggact tgggcacgg caatgcatt 
        tcgtgtcaa cgctcaaaa attaccaga cacaccaaa 
        tcaaacttt ggctcattt ccaacgcca acaaaccgt 
        ttccgacat ctacatctg gacctggtt gggccatta 
        cctccatct catgggtac aaatatgta ctcacctgc 
        atcgacagg ttcacccgc tggccagag gcaattcct 
        ataaaggac atatccgcc gaaaccgtg gcacaagcg 
        ttgctgcac acatggata gcacgcttt ggaattccc 
        gagttaatc accaccgac cgtggcacc cagtttgat 
        tccgaactt tttagaaat atgtcaaaa ctgtttgga 
        atttgccac tgcaaaaca acagcgtat cacccccag 
        gccaatggg atgattgaa agattgcac caacagctg 
        aaaacctcg atcaaatgc cacaacact gaaaactgg 
        tttgattgt ctgcctctt gtgttattg ggaattcgc 
        tctgcagta aaagagaac attaaatct agcgcagct 
        gaaatggtc tacggggaa cccttaagc ctgccaggc 
        gaattcttt cattctcca caagaggag ttgacttcg 
        tccgtgctc gaccggcta cgccgaaaa gttcaagat 
        tttgttgct gtggccccg caacgacat gggcttccc 
        aaaccttcg tgccactgg agctaa
      mRNA sequence
      • >TC031725 RA
        cgaaataac aatggacaa gtttttagt cagaactcc 
        aattgattt acaaaagaa gatgaattc aacagatac 
        agaaacacc catcatggc tggaaagga acggggaga 
        aaattcaga aaataaaac ggcggacag tcagagtga 
        tgatgtgga tatggatat tgatgagga gccggaaac 
        agtgcaaga agaacttgt ggaaacgac aagaataag 
        agaaagtat aatcacaaa tttagtgag acgtggcaa 
        tctttacaa cgacgccac gcaaaaccc aacgataat 
        cgatcgcgt agcccttaa ggtaccacc attctgggc 
        agaagatcc ttccctctg gtttttgca actagaggg 
        acaatttca attaaatag ggtgaccct ggacgccac 
        aaaatattt ttacgtaat tacgaattt ggagacaag 
        gtacttaat ggagatcaa agacgtcat caccaacat 
        ccccgaaac gaacaatca agaacagaa aaccaggcg 
        cctcctgga aagagaaga gatgggtga taggacgcc 
        atctcaatt cctacgcca tttgaggga cctagctgg 
        cgcaaacgt tcatgaatc atttctcag aaccttgtg 
        gacagggcg cctgccgag aaatatgca agccatcct 
        tgcgacgca atcgaaatg caccttgga tgaggtagc 
        ggtgtcggc ggacaaaat ccatgaaac cataccgga 
        ggcaagagt ttttgcaat caacaacaa cgcgaacgc 
        cagtagcgc caccaccgg cactcatgg aaactttga 
        cgcaaccat cagcgccct ctcgcaaca agtgcagga 
        cctttcacg acaactggc agcactgca gacttcgca 
        gtctggcca tgcttcgcg ttttgggac tcgggcgcc 
        atctaggta ccggtcgag gagtcgtag caagccgcg 
        cgatggaaa atgctggtt tcaccaccg ttttggcga 
        caaggcaag gaagtgtga agccccatg ctcctacca 
        aaaaaaaaa acggcacaa gcagtcagt agtggcggc 
        atacgactg cgctcgcaa cacttgccg cctattcat 
        ccgcaatcg tgacgacgg agttccatt tcttattga 
        cacaagtgc cgatgtgag tgttttccc caaaaaact 
        tctgcgtgg atctccacc ggcgtccga cgtggtgct 
        ttttgcggt aaacggtac gcgatttcc aacttacgg 
        actacgaac gctacagct aaatctggg gttgcgtcg 
        tgtatttaa gtggcagtt tattatcgc ggatgtgag 
        ctacgcaat aatcggggc ggactttat tcaccatta 
        caaacttct agtagattt acgcaatag acgcttgat 
        agactcatt aacccaggt gtgcgcgga gtgcgagat 
        acgggcagt agatgtgcc gacagttaa aactctatg 
        cattgcggg cgcgagtga gtaccaccg tttactctt 
        ggaattccc cgcaatcac caagccacc caatattca 
        tcaagagcg aaggcacaa caccgtcca cttcatccg 
        gacaaccga tggtccacc cgtttcatg tcgcccccg 
        gcgcttggc gcctgatcg ctacaaggc tgcaaaaaa 
        ggaattcga tgcaatggt gagagacgg aatcgcaag 
        accctctga cggtaactg gtcctctcc gctccacct 
        tgtgcctaa aaaagatgg caatttgag gccatgcgg 
        cgactatag aagactaaa tgcgcgaac tattccgga 
        tcgttaccc tgtgcccca cattgaaga tttttcaca 
        cgcactcca tggtaagaa agtattttc aacaataga 
        cttggttcg agcatacca ccaaattcc ggtcaaccc 
        tcaagacgt tccgaaaac agcgattac aactccttt 
        tggactttt tgaatacac ccaattgtc atttggatt 
        aagaaacgc gtcgcaaac gtttcaaag atttattta 
        tgaagtcac acatgagct cgacttctg tttcgcata 
        catagatga cgttctcgt cgcatcaga gaactctca 
        acaacattt gaggaaact gttcacacg cttcttaga 
        ctacggcgt tgtaattaa ttctgataa atgcaaatt 
        cggtcaatc agaagtaac gtttctggg ataccacat 
        ttctgacaa gggcaccca acctccaac cgatcgtgt 
        ccaggccat tttagacat gaaaaaacc ttcgacttt 
        gaaagagct gcagcggtt tcttggcat ggtaaattt 
        ttaccgacg tttcattcc acaggcagc cagaattca 
        agcaccgct gacacaact ccaaggggg gcccaaggc 
        caaacaaaa agaaacaat caagtggtg tccagaaac 
        agaaaacgc attcgaagc cacgaaacg atcgcttgc 
        gaacgctgc actgctggc acatccgtc cccagaagc 
        cccattgtc aatatgtat gaatcaaaa cccggagaa 
        agcgtcacc caggcaatt tcgatatct agacttcat 
        aagccaatt tacaactga tatccagca cgtttctgg 
        cgcctcaaa catcccagc agatactct ctcgcgaat 
        cgaaacggt atcaggtgt tcagatcca cttcttcaa 
        acttccaga acttgcaaa agctcaaga agacgacgc 
        cgagctaaa acatctttt ctcctctca gaattcatg 
        ctccctaaa taaattata ttttccaga acacgcctg 
        ttctatagt atgcgacac gtccaccgg gacaattcg 
        accttatct ccccgaggc ctaccggaa acaagtgtt 
        ttataacgt ccacaactt gtcgcatcc aggtgtgaa 
        cgcaacaac aaaaatggt gaagcaaaa atatatgtg 
        gccaggcat cgacaaaaa ttgtcggac ttgggcacg 
        gcaatgcat ttcgtgtca acgctcaaa aattaccag 
        acacaccaa atcaaactt tggctcatt tccaacgcc 
        aacaaaccg tttccgaca tctacatct ggacctggt 
        tgggccatt acctccatc tcatgggta caaatatgt 
        actcacctg catcgacag gttcacccg ctggccaga 
        ggcaattcc tataaagga catatccgc cgaaaccgt 
        ggcacaagc gttgctgca cacatggat agcacgctt 
        tggaattcc cgagttaat caccaccga ccgtggcac 
        ccagtttga ttccgaact ttttagaaa tatgtcaaa 
        actgtttgg aatttgcca ctgcaaaac aacagcgta 
        tcaccccca ggccaatgg gatgattga aagattgca 
        ccaacagct gaaaacctc gatcaaatg ccacaacac 
        tgaaaactg gtttgattg tctgcctct tgtgttatt 
        gggaattcg ctctgcagt aaaagagaa cattaaatc 
        tagcgcagc tgaaatggt ctacgggga acccttaag 
        cctgccagg cgaattctt tcattctcc acaagagga 
        gttgacttc gtccgtgct cgaccggct acgccgaaa 
        agttcaaga ttttgttgc tgtggcccc gcaacgaca 
        tgggcttcc caaaccttc gtgccactg gagctaaag 
        acatgctca cacgttttt atccgacta gcagtggca 
        acaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY