Status
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Gene information
General information
TC011637 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15817693..15821732
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011637
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08956

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011637 RA
      MPIFALQRS YKYMMYATK QPSLRINQA DLKCKNGCG 
      YYGNADWDG YCSKCYRNH MDQERQRKA RQSHSDQTK 
      VSGFSKFEE KKRQQTDKK NKYLKSLPV FRKTSSAKD 
      SGRPERHLD LRQANPDAD KLTAEFISL YGVWGEQVR 
      RDFFKCVQS FTTKIYSEL ENKPIEDIA EMAQKYYNL 
      YSNRVNNTE TYQEVTTDV RDELLDFFE KYVMVSLYS 
      WLFCPPSTN DEEKDLIIQ ERIRKLSWV NAHHLDCCI 
      SETSIEVRD LVYTAITHL LGMDSMKAP QDKLSCVVS 
      CCQSVVKVL QHCQGGPVS ADEFLPALI FIVLKANPA 
      RLKSNILYV TRFCNDSRL MQGEAGYYF TNLCCAVSF 
      IENLTAESL NMPQQEFEA YMNGEITSV SAWESALVA 
      CEGMHQLCE HLSLLKDLS IRTNTVQDE TKKLTDDIL 
      KFKEEMNEK VNSVLERTP LIIKPRKTP ELSEKVDDT 
      QNSQNFYLQ NLQIATSNI DDAKTKLQL DITPCVSKT 
      SPTDDHSLS KINYDIDFS DLSGESSLA ELTPEKRKS 
      PSFTPDPFS PVGSSCPIT QAPLIPTKP DDFVLPFVE 
      TQETLLEKE DEGPNLPSP IKPMTAQYS GFSKQGWQI 
      PSIPCITGE LGANASQAN SQTSQDKEN
    CDS sequence
    • >TC011637 RA
      atgccgata tttgccttg cagcgttct tacaagtac 
      atgatgtac gccaccaaa caaccctct ttaaggatc 
      aatcaagcc gatctcaag tgcaaaaat gggtgcgga 
      tactatgga aacgccgat tgggacggt tactgttca 
      aaatgctac cgcaaccac atggaccag gagcgccaa 
      cgaaaggcg cgccaaagt cactccgac caaaccaaa 
      gtctctggt ttttcgaaa ttcgaagaa aagaagcgc 
      caacaaacc gacaagaag aacaaatat ttgaaaagt 
      ttgcccgtt tttcgcaaa acttcaagc gctaaagat 
      tcgggtcgt ccggagcgt catttggat ttgagacaa 
      gcgaatcca gatgctgat aaattgacg gctgaattt 
      atttcgctc tatggggtt tggggtgaa caagtccga 
      agggacttt ttcaagtgt gttcagtcg ttcacaacg 
      aagatctac agcgagttg gagaataaa ccgatcgag 
      gatattgcc gaaatggcg cagaaatat tacaatttg 
      tacagtaat cgggtcaat aatacggag acgtatcag 
      gaagttaca acagacgtt cgggatgag ttgttggat 
      tttttcgaa aagtacgtc atggtcagt ctttacagt 
      tggctgttt tgcccaccg agcaccaac gacgaagag 
      aaagacttg ataatacaa gaacgcata cgcaagctt 
      agttgggtc aacgcacat catttagac tgttgcatt 
      agcgaaact agtatagaa gttagggat ttagtttac 
      acagccatc acacattta ctcggaatg gattcaatg 
      aaagcacca caagacaag ctgtcttgt gttgtttcc 
      tgttgtcag agtgtcgtc aaagttttg caacattgt 
      caaggaggt ccagttagt gccgatgaa tttttacca 
      gctctcatt ttcatcgtc ttgaaagcg aatccggca 
      cgactgaaa agtaacatt ctttacgta acgcgtttt 
      tgcaacgac agtcgatta atgcaaggg gaagccggt 
      tattatttc actaatttg tgctgcgcc gtttcgttt 
      attgaaaat ttgaccgct gagtcgtta aatatgcct 
      cagcaagag ttcgaggct tacatgaac ggagagata 
      acatcggta agtgcctgg gaatcggca cttgtggca 
      tgcgaaggc atgcaccag ctgtgcgaa cacttgtct 
      ctccttaaa gacttgtcg attcgcacg aacacggtt 
      caagacgaa acgaaaaaa ctaactgac gatatttta 
      aaattcaaa gaggagatg aacgagaaa gttaattcg 
      gttctggag cgcactcca cttatcatc aagccgcgt 
      aaaacccca gaacttagt gaaaaggtt gacgacact 
      caaaacagt caaaatttc tatttgcaa aatctccaa 
      atcgccaca agtaacatc gacgatgcc aaaactaaa 
      ttacaacta gacatcaca ccgtgcgtt agtaaaaca 
      tcgccgact gacgatcac agtctctct aaaatcaac 
      tacgatatc gatttttcc gacttaagt ggcgagagt 
      agtttggct gaattgacc ccagaaaag agaaaatcg 
      ccaagtttc acaccggat cccttcagt ccagttggg 
      agcagttgc ccgataaca caggctcct ttaataccg 
      acaaaaccg gacgatttt gttttaccg ttcgttgaa 
      acgcaagaa actctatta gagaaagag gacgaagga 
      ccgaattta ccatcgccg attaaacca atgacggca 
      caatattcg gggttttcc aaacagggg tggcaaata 
      cccagcatt ccgtgcatt acgggggag ctgggcgcc 
      aatgcatca caagccaat agtcagacg agtcaggat 
      aaggagaat taa
    mRNA sequence
    • >TC011637 RA
      ataaaagat tttttatct catttgaaa aaagtagtt 
      tgggcgctt gttatcgca aggttggca gcactgcga 
      aggtaaaaa atggcgtgc gtcagctgt tattgattt 
      tttgatcaa aacaacaaa cataaaaaa tgaactgtg 
      agcgataaa tttggtaaa aaagtcctt attgctgca 
      aaacgagtg ataaaacag gcgtgttat caccatcac 
      cacaagaaa gatgcaaaa cattttgtt agtgagcga 
      ttgacgaaa cttgctaga cggtgagta aacattaat 
      taatcatcg ctaaatttg ttgtttatc accgtttga 
      aaaaatcat tgacgcgaa tcgtcttca gttatttag 
      cacgaaaac tgtgcctcg ctgcgattg gccgtagat 
      aaggattct tggtcaaat tagactttt aaccttgtc 
      cattcaatt ggttaatcc ttatcgctt gataaactc 
      gttccaata aaagactct ccatcatct tgcaacggc 
      agttttctc gtcttaatc gagccaagc tgtctcgcg 
      tttcacgaa aaaaacaac accgagcgt tcgcccccc 
      gtttcctcc ttgcgtttt tccaaccag caccctgtc 
      gttaacaaa ttcaccccc ttgacaaac cctccacaa 
      atggctgat cctggtgaa agtttaata agtgccttc 
      atcgaatcc ctaacgagg attcacatc gttgaaaca 
      gcgggcatc aactttcta aaattcccc caattccca 
      gtcgtccaa atgctaatg aaagagttg aatttgtgt 
      gcacacgat cacagcttc gtaccaccg tggtaaaat 
      aatcaaaaa ctaaaaacg agtgccttt tatcgtcac 
      tcaacgagt gaaaaatcc acaacagat gggcgattg 
      agaaattca caaagaacg gactcataa actattttc 
      tttagttct taagtagtg ttattagtg gggtgaaaa 
      tgccgatat ttgccttgc agcgttctt acaagtaca 
      tgatgtacg ccaccaaac aaccctctt taaggatca 
      atcaagccg atctcaagt gcaaaaatg ggtgcggat 
      actatggaa acgccgatt gggacggtt actgttcaa 
      aatgctacc gcaaccaca tggaccagg agcgccaac 
      gaaaggcgc gccaaagtc actccgacc aaaccaaag 
      tctctggtt tttcgaaat tcgaagaaa agaagcgcc 
      aacaaaccg acaagaaga acaaatatt tgaaaagtt 
      tgcccgttt ttcgcaaaa cttcaagcg ctaaagatt 
      cgggtcgtc cggagcgtc atttggatt tgagacaag 
      cgaatccag atgctgata aattgacgg ctgaattta 
      tttcgctct atggggttt ggggtgaac aagtccgaa 
      gggactttt tcaagtgtg ttcagtcgt tcacaacga 
      agatctaca gcgagttgg agaataaac cgatcgagg 
      atattgccg aaatggcgc agaaatatt acaatttgt 
      acagtaatc gggtcaata atacggaga cgtatcagg 
      aagttacaa cagacgttc gggatgagt tgttggatt 
      ttttcgaaa agtacgtca tggtcagtc tttacagtt 
      ggctgtttt gcccaccga gcaccaacg acgaagaga 
      aagacttga taatacaag aacgcatac gcaagctta 
      gttgggtca acgcacatc atttagact gttgcatta 
      gcgaaacta gtatagaag ttagggatt tagtttaca 
      cagccatca cacatttac tcggaatgg attcaatga 
      aagcaccac aagacaagc tgtcttgtg ttgtttcct 
      gttgtcaga gtgtcgtca aagttttgc aacattgtc 
      aaggaggtc cagttagtg ccgatgaat ttttaccag 
      ctctcattt tcatcgtct tgaaagcga atccggcac 
      gactgaaaa gtaacattc tttacgtaa cgcgttttt 
      gcaacgaca gtcgattaa tgcaagggg aagccggtt 
      attatttca ctaatttgt gctgcgccg tttcgttta 
      ttgaaaatt tgaccgctg agtcgttaa atatgcctc 
      agcaagagt tcgaggctt acatgaacg gagagataa 
      catcggtaa gtgcctggg aatcggcac ttgtggcat 
      gcgaaggca tgcaccagc tgtgcgaac acttgtctc 
      tccttaaag acttgtcga ttcgcacga acacggttc 
      aagacgaaa cgaaaaaac taactgacg atattttaa 
      aattcaaag aggagatga acgagaaag ttaattcgg 
      ttctggagc gcactccac ttatcatca agccgcgta 
      aaaccccag aacttagtg aaaaggttg acgacactc 
      aaaacagtc aaaatttct atttgcaaa atctccaaa 
      tcgccacaa gtaacatcg acgatgcca aaactaaat 
      tacaactag acatcacac cgtgcgtta gtaaaacat 
      cgccgactg acgatcaca gtctctcta aaatcaact 
      acgatatcg atttttccg acttaagtg gcgagagta 
      gtttggctg aattgaccc cagaaaaga gaaaatcgc 
      caagtttca caccggatc ccttcagtc cagttggga 
      gcagttgcc cgataacac aggctcctt taataccga 
      caaaaccgg acgattttg ttttaccgt tcgttgaaa 
      cgcaagaaa ctctattag agaaagagg acgaaggac 
      cgaatttac catcgccga ttaaaccaa tgacggcac 
      aatattcgg ggttttcca aacaggggt ggcaaatac 
      ccagcattc cgtgcatta cgggggagc tgggcgcca 
      atgcatcac aagccaata gtcagacga gtcaggata 
      aggagaatt aactgtatc aatgtttta aatatattt 
      tattcccaa tt
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