| General information | |||
| TC012618 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12201344..12208189 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012618 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08953 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012618 RA
MHTHRHYEE ENEWGCGSW LEYALVMIL ACILLLASL ALTLYWVIK HRGGFAWRD DPIKQFNFH PVLMVAGFI TFSGFSILL YRICRCCRR IYVKLLHTV FHALAIPCV AVGFLTVLD SHNLAQPPI PNFYSLHSW IGLVTMGLF AIQFVVGFF SFLILLCCE GATAGFRAS LVPIHASFG LTTFMLAIA ACLTGLTEK VFFSLSSYY TTFPPEGNV VNALGMVLV ALAIVTGYI LQREEFRFR AHILIRSGD L
- >TC012618 RB
MAKNKMHTH RHYEEENEW GCGSWLEYA LVMILACIL LLASLALTL YWVIKHRGG FAWRDDPIK QFNFHPVLM VAGFITFSG FSILLYRIC RCCRRIYVK LLHTVFHAL AIPCVAVGF LTVLDSHNL AQPPIPNFY SLHSWIGLV TMGLFAIQF VVGFFSFLI LLCCEGATA GFRASLVPI HASFGLTTF MLAIAACLT GLTEKVFFS LSSYYTTFP PEGNVVNAL GMVLVALAI VTGYILQRE EFRFRAHIL IRSGDL
- >TC012618 RA
atgcacaca caccggcac tacgaggag gagaacgaa tggggctgc ggcagctgg ctggagtac gccttggtg atgatcctg gcctgcatc ctcctcctg gcctcgctg gctctgacc ctgtactgg gtgatcaaa catcgagga ggtttcgca tggcgagac gaccccatc aagcagttc aacttccat ccggtcctc atggtggcc ggcttcatc acattcagc ggattctcc atcctcttg tacagaata tgtcggtgt tgccggaga atctacgtc aagctcctc cacaccgtg tttcacgct ttggcgatt ccctgcgtc gccgtcggt ttcttgacg gttctggat tcccacaat ctcgcccag ccgcccatc cccaacttc tactccctg cacagctgg atcggattg gtaacaatg ggcctgttc gctatacag ttcgtcgtg ggtttcttc agtttcctc atcctgttg tgttgcgaa ggggcgacg gcaggcttt cgggcgtct ctggttcct atccatgcc tcgttcggg ctgacgacg ttcatgttg gccattgcg gcgtgtctg actggcctc actgagaaa gtgttcttc tcgctgtca tcgtactat accacattc cctcccgaa ggaaatgtt gtaaatgcc ttaggaatg gtccttgtg gcgctagct attgttaca ggatatatc ctgcagaga gaggagttc cggttccgg gcgcacatc ctcatccgt tccggcgac ctatag
- >TC012618 RB
atggcgaag aataagatg cacacacac cggcactac gaggaggag aacgaatgg ggctgcggc agctggctg gagtacgcc ttggtgatg atcctggcc tgcatcctc ctcctggcc tcgctggct ctgaccctg tactgggtg atcaaacat cgaggaggt ttcgcatgg cgagacgac cccatcaag cagttcaac ttccatccg gtcctcatg gtggccggc ttcatcaca ttcagcgga ttctccatc ctcttgtac agaatatgt cggtgttgc cggagaatc tacgtcaag ctcctccac accgtgttt cacgctttg gcgattccc tgcgtcgcc gtcggtttc ttgacggtt ctggattcc cacaatctc gcccagccg cccatcccc aacttctac tccctgcac agctggatc ggattggta acaatgggc ctgttcgct atacagttc gtcgtgggt ttcttcagt ttcctcatc ctgttgtgt tgcgaaggg gcgacggca ggctttcgg gcgtctctg gttcctatc catgcctcg ttcgggctg acgacgttc atgttggcc attgcggcg tgtctgact ggcctcact gagaaagtg ttcttctcg ctgtcatcg tactatacc acattccct cccgaagga aatgttgta aatgcctta ggaatggtc cttgtggcg ctagctatt gttacagga tatatcctg cagagagag gagttccgg ttccgggcg cacatcctc atccgttcc ggcgaccta tag
- >TC012618 RA
gtgaccctc cgagattcc accaaatcg tctagccgt tcgttatca aaagccctt cttcacagc agatgtcgc catccaaac aaaacagaa aagtcgact ctagcgagc cgcgccagc tctcgcgca gtcccgctc taaactcaa aagcagcag caaagtggg tttcttcgc tctgcctct tcgtcttcc ttttattta gttcgcttg cggtcgttg tgcgtggtg tatgtgcgc atgttgtgt aaacaaagt gcacgtttg aaattttcc cgaatttga tttttccaa aacttggac catggcgaa gaataagat gcacacaca ccggcacta cgaggagga gaacgaatg gggctgcgg cagctggct ggagtacgc cttggtgat gatcctggc ctgcatcct cctcctggc ctcgctggc tctgaccct gtactgggt gatcaaaca tcgaggagg tttcgcatg gcgagacga ccccatcaa gcagttcaa cttccatcc ggtcctcat ggtggccgg cttcatcac attcagcgg attctccat cctcttgta cagaatatg tcggtgttg ccggagaat ctacgtcaa gctcctcca caccgtgtt tcacgcttt ggcgattcc ctgcgtcgc cgtcggttt cttgacggt tctggattc ccacaatct cgcccagcc gcccatccc caacttcta ctccctgca cagctggat cggattggt aacaatggg cctgttcgc tatacagtt cgtcgtggg tttcttcag tttcctcat cctgttgtg ttgcgaagg ggcgacggc aggctttcg ggcgtctct ggttcctat ccatgcctc gttcgggct gacgacgtt catgttggc cattgcggc gtgtctgac tggcctcac tgagaaagt gttcttctc gctgtcatc gtactatac cacattccc tcccgaagg aaatgttgt aaatgcctt aggaatggt ccttgtggc gctagctat tgttacagg atatatcct gcagagaga ggagttccg gttccgggc gcacatcct catccgttc cggcgacct atagtttcg catgatcga aatttttta gtgataatg atttttgta ctgttttta tacatgaac caatacaac actaacaat ttcctgcat gctctctct tctttttgc atgatgctt ggacgatcc gagtctggc gctttgtaa ataagccaa aattgttca ctactaaca atttcaggg atgattacg ttaagagtc cgttggaag ctggggaag aagggatag tagtgaaca ctattgcga tggtgctgg tggcacttg gtatcttag tatctttcg ctgttcgta gtccgacac ttcgtggtg atgcaaagg tgtgtgtta ctgaaagat tgtaggaca gtgcgcttt caagcgcca aagtctcgg atgtgaacg cctctcaac aacccgaac gtttctaac accaactaa aggactctc aaacagaca tgattttgc atgtgtaaa ttgtgtttg ttttggtgt tcatttttt cataaaaag tatgccaaa atagagatc agcattact aaatagcat cgtagcgta gctaatcgt cgggagatc gaacacacc gccgtgtgc tctttcgtc cctctgttt taattttct cattatctt taaatatta accagtcta ttaacattg tacagaaac gaaatttgt taacacgaa agctcaaga cctactgaa tgtattata atcaaatac tcatagctg tgtgtgcta ttctattaa gactataac tataataga gcattttat aaatattta aggatttgt tgagatatt tatattacg ttttataga acaatgtag gatgtttgt tgtcgtagc tcttaaaaa cgagaagca atctgtttc ctgtttcat aacgtaacg aaatttgat gttcaacta aaccaatta gaggaaatt acattagtg ttcaagatt tgtgaatgt taacttcca attccgtat aatagaaaa gtacaattt ctttaatgg accaatagg aatttaacg aagcgcgtt catacaatg aaaatttgt tgtttcttc tatatttgt tatatttat atttttatt ccagtttat tgagtaata tcatagagt taatatatg acttgcaag ataaagtat gtgtacaat tatttataa gttttaata aatgtatca aaattta
- >TC012618 RB
gtgaccctc cgagattcc accaaatcg tctagccgt tcgttatca aaagccctt cttcacagc agatgtcgc catccaaac aaaacagaa aagtcgact ctagcgagc cgcgccagc tctcgcgca gtcccgctc taaactcaa aagcagcag caaagtggg tttcttcgc tctgcctct tcgtcttcc ttttattta gttcgcttg cggtcgttg tgcgtggtg tatgtgcgc atgttgtgt aaacaaagt gcacgtttg aaattttcc cgaatttga tttttccaa aacttggac catggcgaa gaataagat gcacacaca ccggcacta cgaggagga gaacgaatg gggctgcgg cagctggct ggagtacgc cttggtgat gatcctggc ctgcatcct cctcctggc ctcgctggc tctgaccct gtactgggt gatcaaaca tcgaggagg tttcgcatg gcgagacga ccccatcaa gcagttcaa cttccatcc ggtcctcat ggtggccgg cttcatcac attcagcgg attctccat cctcttgta cagaatatg tcggtgttg ccggagaat ctacgtcaa gctcctcca caccgtgtt tcacgcttt ggcgattcc ctgcgtcgc cgtcggttt cttgacggt tctggattc ccacaatct cgcccagcc gcccatccc caacttcta ctccctgca cagctggat cggattggt aacaatggg cctgttcgc tatacagtt cgtcgtggg tttcttcag tttcctcat cctgttgtg ttgcgaagg ggcgacggc aggctttcg ggcgtctct ggttcctat ccatgcctc gttcgggct gacgacgtt catgttggc cattgcggc gtgtctgac tggcctcac tgagaaagt gttcttctc gctgtcatc gtactatac cacattccc tcccgaagg aaatgttgt aaatgcctt aggaatggt ccttgtggc gctagctat tgttacagg atatatcct gcagagaga ggagttccg gttccgggc gcacatcct catccgttc cggcgacct atagtttcg catgatcga aatttttta gtgataatg atttttgta ctgttttta tacatgaac caatacaac actaacaat ttcctgcat gctctctct tctttttgc atgatgctt ggacgatcc gagtctggc gctttgtaa ataagccaa aattgttca ctactaaca atttcaggg atgattacg ttaagagtc cgttggaag ctggggaag aagggatag tagtgaaca ctattgcga tggtgctgg tggcacttg gtatcttag tatctttcg ctgttcgta gtccgacac ttcgtggtg atgcaaagg tgtgtgtta ctgaaagat tgtaggaca gtgcgcttt caagcgcca aagtctcgg atgtgaacg cctctcaac aacccgaac gtttctaac accaactaa aggactctc aaacagaca tgattttgc atgtgtaaa ttgtgtttg ttttggtgt tcatttttt cataaaaag tatgccaaa atagagatc agcattact aaatagcat cgtagcgta gctaatcgt cgggagatc gaacacacc gccgtgtgc tctttcgtc cctctgttt taattttct cattatctt taaatatta accagtcta ttaacattg tacagaaac gaaatttgt taacacgaa agctcaaga cctactgaa tgtattata atcaaatac tcatagctg tgtgtgcta ttctattaa gactataac tataataga gcattttat aaatattta aggatttgt tgagatatt tatattacg ttttataga acaatgtag gatgtttgt tgtcgtagc tcttaaaaa cgagaagca atctgtttc ctgtttcat aacgtaacg aaatttgat gttcaacta aaccaatta gaggaaatt acattagtg ttcaagatt tgtgaatgt taacttcca attccgtat aatagaaaa gtacaattt ctttaatgg accaatagg aatttaacg aagcgcgtt catacaatg aaaatttgt tgtttcttc tatatttgt tatatttat atttttatt ccagtttat tgagtaata tcatagagt taatatatg acttgcaag ataaagtat gtgtacaat tatttataa gttttaata aatgtatca aaattta
