Status
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Gene information
General information
TC012618 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12201344..12208189
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012618
    iBeetle screen iB_08953

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012618 RA
      MHTHRHYEE ENEWGCGSW LEYALVMIL ACILLLASL 
ALTLYWVIK HRGGFAWRD DPIKQFNFH PVLMVAGFI 
TFSGFSILL YRICRCCRR IYVKLLHTV FHALAIPCV 
AVGFLTVLD SHNLAQPPI PNFYSLHSW IGLVTMGLF 
AIQFVVGFF SFLILLCCE GATAGFRAS LVPIHASFG 
LTTFMLAIA ACLTGLTEK VFFSLSSYY TTFPPEGNV 
VNALGMVLV ALAIVTGYI LQREEFRFR AHILIRSGD 
L
    • >TC012618 RB
      MAKNKMHTH RHYEEENEW GCGSWLEYA LVMILACIL 
LLASLALTL YWVIKHRGG FAWRDDPIK QFNFHPVLM 
VAGFITFSG FSILLYRIC RCCRRIYVK LLHTVFHAL 
AIPCVAVGF LTVLDSHNL AQPPIPNFY SLHSWIGLV 
TMGLFAIQF VVGFFSFLI LLCCEGATA GFRASLVPI 
HASFGLTTF MLAIAACLT GLTEKVFFS LSSYYTTFP 
PEGNVVNAL GMVLVALAI VTGYILQRE EFRFRAHIL 
IRSGDL
    CDS sequence
    • >TC012618 RA
      atgcacaca caccggcac tacgaggag gagaacgaa 
tggggctgc ggcagctgg ctggagtac gccttggtg 
atgatcctg gcctgcatc ctcctcctg gcctcgctg 
gctctgacc ctgtactgg gtgatcaaa catcgagga 
ggtttcgca tggcgagac gaccccatc aagcagttc 
aacttccat ccggtcctc atggtggcc ggcttcatc 
acattcagc ggattctcc atcctcttg tacagaata 
tgtcggtgt tgccggaga atctacgtc aagctcctc 
cacaccgtg tttcacgct ttggcgatt ccctgcgtc 
gccgtcggt ttcttgacg gttctggat tcccacaat 
ctcgcccag ccgcccatc cccaacttc tactccctg 
cacagctgg atcggattg gtaacaatg ggcctgttc 
gctatacag ttcgtcgtg ggtttcttc agtttcctc 
atcctgttg tgttgcgaa ggggcgacg gcaggcttt 
cgggcgtct ctggttcct atccatgcc tcgttcggg 
ctgacgacg ttcatgttg gccattgcg gcgtgtctg 
actggcctc actgagaaa gtgttcttc tcgctgtca 
tcgtactat accacattc cctcccgaa ggaaatgtt 
gtaaatgcc ttaggaatg gtccttgtg gcgctagct 
attgttaca ggatatatc ctgcagaga gaggagttc 
cggttccgg gcgcacatc ctcatccgt tccggcgac 
ctatag
    • >TC012618 RB
      atggcgaag aataagatg cacacacac cggcactac 
gaggaggag aacgaatgg ggctgcggc agctggctg 
gagtacgcc ttggtgatg atcctggcc tgcatcctc 
ctcctggcc tcgctggct ctgaccctg tactgggtg 
atcaaacat cgaggaggt ttcgcatgg cgagacgac 
cccatcaag cagttcaac ttccatccg gtcctcatg 
gtggccggc ttcatcaca ttcagcgga ttctccatc 
ctcttgtac agaatatgt cggtgttgc cggagaatc 
tacgtcaag ctcctccac accgtgttt cacgctttg 
gcgattccc tgcgtcgcc gtcggtttc ttgacggtt 
ctggattcc cacaatctc gcccagccg cccatcccc 
aacttctac tccctgcac agctggatc ggattggta 
acaatgggc ctgttcgct atacagttc gtcgtgggt 
ttcttcagt ttcctcatc ctgttgtgt tgcgaaggg 
gcgacggca ggctttcgg gcgtctctg gttcctatc 
catgcctcg ttcgggctg acgacgttc atgttggcc 
attgcggcg tgtctgact ggcctcact gagaaagtg 
ttcttctcg ctgtcatcg tactatacc acattccct 
cccgaagga aatgttgta aatgcctta ggaatggtc 
cttgtggcg ctagctatt gttacagga tatatcctg 
cagagagag gagttccgg ttccgggcg cacatcctc 
atccgttcc ggcgaccta tag
    mRNA sequence
    • >TC012618 RA
      gtgaccctc cgagattcc accaaatcg tctagccgt 
tcgttatca aaagccctt cttcacagc agatgtcgc 
catccaaac aaaacagaa aagtcgact ctagcgagc 
cgcgccagc tctcgcgca gtcccgctc taaactcaa 
aagcagcag caaagtggg tttcttcgc tctgcctct 
tcgtcttcc ttttattta gttcgcttg cggtcgttg 
tgcgtggtg tatgtgcgc atgttgtgt aaacaaagt 
gcacgtttg aaattttcc cgaatttga tttttccaa 
aacttggac catggcgaa gaataagat gcacacaca 
ccggcacta cgaggagga gaacgaatg gggctgcgg 
cagctggct ggagtacgc cttggtgat gatcctggc 
ctgcatcct cctcctggc ctcgctggc tctgaccct 
gtactgggt gatcaaaca tcgaggagg tttcgcatg 
gcgagacga ccccatcaa gcagttcaa cttccatcc 
ggtcctcat ggtggccgg cttcatcac attcagcgg 
attctccat cctcttgta cagaatatg tcggtgttg 
ccggagaat ctacgtcaa gctcctcca caccgtgtt 
tcacgcttt ggcgattcc ctgcgtcgc cgtcggttt 
cttgacggt tctggattc ccacaatct cgcccagcc 
gcccatccc caacttcta ctccctgca cagctggat 
cggattggt aacaatggg cctgttcgc tatacagtt 
cgtcgtggg tttcttcag tttcctcat cctgttgtg 
ttgcgaagg ggcgacggc aggctttcg ggcgtctct 
ggttcctat ccatgcctc gttcgggct gacgacgtt 
catgttggc cattgcggc gtgtctgac tggcctcac 
tgagaaagt gttcttctc gctgtcatc gtactatac 
cacattccc tcccgaagg aaatgttgt aaatgcctt 
aggaatggt ccttgtggc gctagctat tgttacagg 
atatatcct gcagagaga ggagttccg gttccgggc 
gcacatcct catccgttc cggcgacct atagtttcg 
catgatcga aatttttta gtgataatg atttttgta 
ctgttttta tacatgaac caatacaac actaacaat 
ttcctgcat gctctctct tctttttgc atgatgctt 
ggacgatcc gagtctggc gctttgtaa ataagccaa 
aattgttca ctactaaca atttcaggg atgattacg 
ttaagagtc cgttggaag ctggggaag aagggatag 
tagtgaaca ctattgcga tggtgctgg tggcacttg 
gtatcttag tatctttcg ctgttcgta gtccgacac 
ttcgtggtg atgcaaagg tgtgtgtta ctgaaagat 
tgtaggaca gtgcgcttt caagcgcca aagtctcgg 
atgtgaacg cctctcaac aacccgaac gtttctaac 
accaactaa aggactctc aaacagaca tgattttgc 
atgtgtaaa ttgtgtttg ttttggtgt tcatttttt 
cataaaaag tatgccaaa atagagatc agcattact 
aaatagcat cgtagcgta gctaatcgt cgggagatc 
gaacacacc gccgtgtgc tctttcgtc cctctgttt 
taattttct cattatctt taaatatta accagtcta 
ttaacattg tacagaaac gaaatttgt taacacgaa 
agctcaaga cctactgaa tgtattata atcaaatac 
tcatagctg tgtgtgcta ttctattaa gactataac 
tataataga gcattttat aaatattta aggatttgt 
tgagatatt tatattacg ttttataga acaatgtag 
gatgtttgt tgtcgtagc tcttaaaaa cgagaagca 
atctgtttc ctgtttcat aacgtaacg aaatttgat 
gttcaacta aaccaatta gaggaaatt acattagtg 
ttcaagatt tgtgaatgt taacttcca attccgtat 
aatagaaaa gtacaattt ctttaatgg accaatagg 
aatttaacg aagcgcgtt catacaatg aaaatttgt 
tgtttcttc tatatttgt tatatttat atttttatt 
ccagtttat tgagtaata tcatagagt taatatatg 
acttgcaag ataaagtat gtgtacaat tatttataa 
gttttaata aatgtatca aaattta
    • >TC012618 RB
      gtgaccctc cgagattcc accaaatcg tctagccgt 
tcgttatca aaagccctt cttcacagc agatgtcgc 
catccaaac aaaacagaa aagtcgact ctagcgagc 
cgcgccagc tctcgcgca gtcccgctc taaactcaa 
aagcagcag caaagtggg tttcttcgc tctgcctct 
tcgtcttcc ttttattta gttcgcttg cggtcgttg 
tgcgtggtg tatgtgcgc atgttgtgt aaacaaagt 
gcacgtttg aaattttcc cgaatttga tttttccaa 
aacttggac catggcgaa gaataagat gcacacaca 
ccggcacta cgaggagga gaacgaatg gggctgcgg 
cagctggct ggagtacgc cttggtgat gatcctggc 
ctgcatcct cctcctggc ctcgctggc tctgaccct 
gtactgggt gatcaaaca tcgaggagg tttcgcatg 
gcgagacga ccccatcaa gcagttcaa cttccatcc 
ggtcctcat ggtggccgg cttcatcac attcagcgg 
attctccat cctcttgta cagaatatg tcggtgttg 
ccggagaat ctacgtcaa gctcctcca caccgtgtt 
tcacgcttt ggcgattcc ctgcgtcgc cgtcggttt 
cttgacggt tctggattc ccacaatct cgcccagcc 
gcccatccc caacttcta ctccctgca cagctggat 
cggattggt aacaatggg cctgttcgc tatacagtt 
cgtcgtggg tttcttcag tttcctcat cctgttgtg 
ttgcgaagg ggcgacggc aggctttcg ggcgtctct 
ggttcctat ccatgcctc gttcgggct gacgacgtt 
catgttggc cattgcggc gtgtctgac tggcctcac 
tgagaaagt gttcttctc gctgtcatc gtactatac 
cacattccc tcccgaagg aaatgttgt aaatgcctt 
aggaatggt ccttgtggc gctagctat tgttacagg 
atatatcct gcagagaga ggagttccg gttccgggc 
gcacatcct catccgttc cggcgacct atagtttcg 
catgatcga aatttttta gtgataatg atttttgta 
ctgttttta tacatgaac caatacaac actaacaat 
ttcctgcat gctctctct tctttttgc atgatgctt 
ggacgatcc gagtctggc gctttgtaa ataagccaa 
aattgttca ctactaaca atttcaggg atgattacg 
ttaagagtc cgttggaag ctggggaag aagggatag 
tagtgaaca ctattgcga tggtgctgg tggcacttg 
gtatcttag tatctttcg ctgttcgta gtccgacac 
ttcgtggtg atgcaaagg tgtgtgtta ctgaaagat 
tgtaggaca gtgcgcttt caagcgcca aagtctcgg 
atgtgaacg cctctcaac aacccgaac gtttctaac 
accaactaa aggactctc aaacagaca tgattttgc 
atgtgtaaa ttgtgtttg ttttggtgt tcatttttt 
cataaaaag tatgccaaa atagagatc agcattact 
aaatagcat cgtagcgta gctaatcgt cgggagatc 
gaacacacc gccgtgtgc tctttcgtc cctctgttt 
taattttct cattatctt taaatatta accagtcta 
ttaacattg tacagaaac gaaatttgt taacacgaa 
agctcaaga cctactgaa tgtattata atcaaatac 
tcatagctg tgtgtgcta ttctattaa gactataac 
tataataga gcattttat aaatattta aggatttgt 
tgagatatt tatattacg ttttataga acaatgtag 
gatgtttgt tgtcgtagc tcttaaaaa cgagaagca 
atctgtttc ctgtttcat aacgtaacg aaatttgat 
gttcaacta aaccaatta gaggaaatt acattagtg 
ttcaagatt tgtgaatgt taacttcca attccgtat 
aatagaaaa gtacaattt ctttaatgg accaatagg 
aatttaacg aagcgcgtt catacaatg aaaatttgt 
tgtttcttc tatatttgt tatatttat atttttatt 
ccagtttat tgagtaata tcatagagt taatatatg 
acttgcaag ataaagtat gtgtacaat tatttataa 
gttttaata aatgtatca aaattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY