Status
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Gene information
General information
TC012023 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7708157..7718274
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012023
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08949

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: 3 / 5 larvae on slide
    maxilla number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012023 RA
      MNHEPLRYA HEEGHTDVC FSTDGSKFI TCGADGDIR 
      IWPSAEDTD PTHTCIGEW SLSVCQKGE CLYVATGSN 
      DVQILTFPG GERNGLLDR FVAPINQIV PSKDSEAVA 
      LAGEDMQVR VVELGAEKR VGVFDGLGG PCLCVTATS 
      KFLAASSGD GKLRIWDVA SKELVKEIA CFPKTNSFA 
      NAEYLCRID FAPNGKYLA YPENDSVVI LDTSDWSQY 
      GILKTNEVT NHYSIVQFS PCGGFLLAT TLKGDFVIF 
      NVSIKMVRG TSKHPNGTP ICGAVWNPS GNGQIVYTD 
      TQGQLGIMS DCISIKTTD DEIFTNNDL NFDDVQFES 
      DDEDNENAI SVEKLKKEV MGDVEPELN FDNASSAPT 
      PRPKTPEIP LQAPFMPSS SPEHLNPRY LCWNHLGIV 
      RCYGSTNDD DSGKSIEVE FLDSSFHHS MMLQNYQDY 
      FLGTVSKAA LAVANTKQL YVIPLNAGS KEWVLKLEE 
      DGTEEIVLV SASENLICF ATNSYIVRV CSIFGTQRG 
      IVSVPGPLV SMASFQNSV LVAYHSGSV RNGDQCINI 
      KLIAFNGLS MHSCDIGAA LGPESTLTW LGFTDFGTP 
      AMVDSLGMV SLFPPNCNI WIPFCDTAR HMKSPSDCF 
      FVTAVLESY QSVVGLKCR GTAYPSFTP TPTLSELIL 
      EPPFAEPTT DKTQLEMSL FTLSNLQVS NQEKKFSEA 
      GLKAFALAC KNNLDQRAV ELMEVLSNR QLVNLSTKY 
      ALKLNKKIL AEKLMEMAG RLMNDDDDS TNVVVDTPT 
      SSPLAPKKV TLSSLKRTP KTKQVVKEV VTAPEASQE 
      SQSSILSPS FPIQEFSQE SPNPFSKSA RKGNESSNP 
      LSLTDKYAG VDYVDAKEN KMKNKETEK RKQPDSETD 
      KPREKQRKL DRFMFSKRT
    CDS sequence
    • >TC012023 RA
      atgaaccac gaaccctta cgctacgcc cacgaggag 
      ggccacacg gacgtgtgc ttctcaacc gacggcagc 
      aaatttatt acgtgtggg gctgacgga gacatccgg 
      atctggccg tcagcggaa gacaccgac cccactcac 
      acttgcata ggcgagtgg tcgctcagt gtgtgccag 
      aaaggggag tgcttgtac gtggccacc ggcagcaat 
      gacgtccaa atcctcacc tttccaggg ggcgaacgg 
      aacgggctt ctggaccgg ttcgtggcc cccatcaac 
      cagatcgtg cccagtaaa gacagcgag gctgtggcg 
      cttgccggt gaagatatg caagtgagg gtggttgag 
      ttgggggct gagaaaaga gttggggtt tttgatggg 
      ttagggggg ccttgtttg tgtgtcacg gctactagt 
      aagtttcta gcggcgagt tctggtgac ggaaaattg 
      aggatttgg gatgttgcc agtaaggaa ctggttaaa 
      gaaattgcc tgttttcct aaaactaac agttttgcc 
      aacgctgaa tacttatgt cgtattgac tttgccccc 
      aatggcaaa tatctggca tatccggaa aacgattca 
      gttgttatt ttagacact tctgattgg tcacagtat 
      ggtatttta aaaacaaat gaagtcacg aatcattat 
      tcaattgta cagttttca ccctgtggc ggttttctt 
      ctagcgaca actcttaaa ggtgacttt gtcattttt 
      aatgttagc attaaaatg gtgagaggg actagtaaa 
      caccctaat ggaacacca atttgtgga gccgtgtgg 
      aacccctca ggaaacggc caaatcgtc tacaccgac 
      actcaaggc cagttaggt attatgtca gattgtata 
      agcatcaaa acaaccgac gacgaaatt ttcaccaat 
      aacgaccta aacttcgac gacgtgcaa ttcgaatca 
      gacgatgaa gacaacgaa aatgccatt tcggtcgaa 
      aaactaaaa aaagaggtc atgggcgac gtagaaccc 
      gagttaaat ttcgacaat gcatcttcg gcgcctact 
      ccgcgcccc aaaaccccc gaaatcccg ctccaagcc 
      ccatttatg cccagctcc agccctgag cacttgaat 
      ccgcgctac ttgtgctgg aaccacctc ggaattgtg 
      cgatgttac ggttccacg aacgacgac gattcggga 
      aaatcaatc gaagttgag tttctcgat tcttcgttc 
      catcacagc atgatgttg caaaactat caggactat 
      ttcttgggg actgtcagt aaagcggcc ctcgctgtg 
      gccaacaca aaacaactt tacgtcatt cccctgaat 
      gcgggctca aaggagtgg gttcttaaa ttggaggaa 
      gacggcacg gaagagatt gttttagtt tcagcttct 
      gaaaatttg atttgtttc gcgacaaac agttacata 
      gttagagtt tgctcgatt ttcggcact cagagagga 
      atcgtttcg gttcctgga cctttagtt tccatggct 
      agtttccag aattcggta cttgtggcc taccattcg 
      ggaagtgtc cgcaacggt gaccagtgt attaacatc 
      aaattgatt gcttttaat ggattgtcg atgcacagt 
      tgcgatatc ggcgcggct ttaggcccc gagtccact 
      ctgacgtgg ctgggattc accgatttt gggactcct 
      gctatggtg gactcgctc ggaatggtc agtcttttt 
      ccccccaat tgtaacatt tggatacct ttttgtgac 
      actgccaga catatgaaa tcgccttct gactgtttt 
      ttcgtgact gccgtcctt gaatcgtat cagtcggtt 
      gttgggctc aagtgccgc ggcactgct tatccgagc 
      ttcacccca acaccgaca ctcagtgaa ttgattttg 
      gagccccca ttcgctgaa cccacaacg gataaaaca 
      cagttggaa atgagtctt tttacgctt tcgaatttg 
      caagtcagt aatcaggag aagaaattt tcagaggct 
      gggttaaaa gcatttgcg ctagcatgt aagaacaat 
      ctcgatcag agggcagtc gaacttatg gaagtttta 
      tccaatcgt cagttggtg aatttgtca acgaaatac 
      gcccttaaa ttgaataaa aaaatattg gcggagaaa 
      ttgatggag atggctgga cgtctcatg aatgacgat 
      gacgattca acgaatgtt gttgtggac acgccgaca 
      tcttcaccc ctcgcccct aaaaaagtg acccttagt 
      tcacttaaa cgtacgcca aaaaccaaa caagtcgtc 
      aaagaagtg gtgacggca ccagaagcc agtcaagag 
      agccaaagt tctatttta agcccgtct ttcccaatc 
      caagaattt tcacaagaa tctccaaat ccattttcc 
      aaaagtgca cgaaaaggg aacgaatca tcgaaccct 
      ctgagtctc actgataaa tacgcgggt gttgattac 
      gtcgatgct aaggaaaat aaaatgaag aacaaggag 
      actgaaaag aggaagcaa cccgattct gagactgac 
      aagccgcgt gaaaaacag agaaaacta gataggttt 
      atgtttagt aaacgcacg tga
    mRNA sequence
    • >TC012023 RA
      tctagttat attccaaac accacattg gcgccactt 
      cccgctttc gcgcctttt tccaacgtc aacaaaccc 
      taacctgaa acattcaaa aaatgaacc acgaaccct 
      tacgctacg cccacgagg agggccaca cggacgtgt 
      gcttctcaa ccgacggca gcaaattta ttacgtgtg 
      gggctgacg gagacatcc ggatctggc cgtcagcgg 
      aagacaccg accccactc acacttgca taggcgagt 
      ggtcgctca gtgtgtgcc agaaagggg agtgcttgt 
      acgtggcca ccggcagca atgacgtcc aaatcctca 
      cctttccag ggggcgaac ggaacgggc ttctggacc 
      ggttcgtgg cccccatca accagatcg tgcccagta 
      aagacagcg aggctgtgg cgcttgccg gtgaagata 
      tgcaagtga gggtggttg agttggggg ctgagaaaa 
      gagttgggg tttttgatg ggttagggg ggccttgtt 
      tgtgtgtca cggctacta gtaagtttc tagcggcga 
      gttctggtg acggaaaat tgaggattt gggatgttg 
      ccagtaagg aactggtta aagaaattg cctgttttc 
      ctaaaacta acagttttg ccaacgctg aatacttat 
      gtcgtattg actttgccc ccaatggca aatatctgg 
      catatccgg aaaacgatt cagttgtta ttttagaca 
      cttctgatt ggtcacagt atggtattt taaaaacaa 
      atgaagtca cgaatcatt attcaattg tacagtttt 
      caccctgtg gcggttttc ttctagcga caactctta 
      aaggtgact ttgtcattt ttaatgtta gcattaaaa 
      tggtgagag ggactagta aacacccta atggaacac 
      caatttgtg gagccgtgt ggaacccct caggaaacg 
      gccaaatcg tctacaccg acactcaag gccagttag 
      gtattatgt cagattgta taagcatca aaacaaccg 
      acgacgaaa ttttcacca ataacgacc taaacttcg 
      acgacgtgc aattcgaat cagacgatg aagacaacg 
      aaaatgcca tttcggtcg aaaaactaa aaaaagagg 
      tcatgggcg acgtagaac ccgagttaa atttcgaca 
      atgcatctt cggcgccta ctccgcgcc ccaaaaccc 
      ccgaaatcc cgctccaag ccccattta tgcccagct 
      ccagccctg agcacttga atccgcgct acttgtgct 
      ggaaccacc tcggaattg tgcgatgtt acggttcca 
      cgaacgacg acgattcgg gaaaatcaa tcgaagttg 
      agtttctcg attcttcgt tccatcaca gcatgatgt 
      tgcaaaact atcaggact atttcttgg ggactgtca 
      gtaaagcgg ccctcgctg tggccaaca caaaacaac 
      tttacgtca ttcccctga atgcgggct caaaggagt 
      gggttctta aattggagg aagacggca cggaagaga 
      ttgttttag tttcagctt ctgaaaatt tgatttgtt 
      tcgcgacaa acagttaca tagttagag tttgctcga 
      ttttcggca ctcagagag gaatcgttt cggttcctg 
      gacctttag tttccatgg ctagtttcc agaattcgg 
      tacttgtgg cctaccatt cgggaagtg tccgcaacg 
      gtgaccagt gtattaaca tcaaattga ttgctttta 
      atggattgt cgatgcaca gttgcgata tcggcgcgg 
      ctttaggcc ccgagtcca ctctgacgt ggctgggat 
      tcaccgatt ttgggactc ctgctatgg tggactcgc 
      tcggaatgg tcagtcttt ttcccccca attgtaaca 
      tttggatac ctttttgtg acactgcca gacatatga 
      aatcgcctt ctgactgtt ttttcgtga ctgccgtcc 
      ttgaatcgt atcagtcgg ttgttgggc tcaagtgcc 
      gcggcactg cttatccga gcttcaccc caacaccga 
      cactcagtg aattgattt tggagcccc cattcgctg 
      aacccacaa cggataaaa cacagttgg aaatgagtc 
      tttttacgc tttcgaatt tgcaagtca gtaatcagg 
      agaagaaat tttcagagg ctgggttaa aagcatttg 
      cgctagcat gtaagaaca atctcgatc agagggcag 
      tcgaactta tggaagttt tatccaatc gtcagttgg 
      tgaatttgt caacgaaat acgccctta aattgaata 
      aaaaaatat tggcggaga aattgatgg agatggctg 
      gacgtctca tgaatgacg atgacgatt caacgaatg 
      ttgttgtgg acacgccga catcttcac ccctcgccc 
      ctaaaaaag tgaccctta gttcactta aacgtacgc 
      caaaaacca aacaagtcg tcaaagaag tggtgacgg 
      caccagaag ccagtcaag agagccaaa gttctattt 
      taagcccgt ctttcccaa tccaagaat tttcacaag 
      aatctccaa atccatttt ccaaaagtg cacgaaaag 
      ggaacgaat catcgaacc ctctgagtc tcactgata 
      aatacgcgg gtgttgatt acgtcgatg ctaaggaaa 
      ataaaatga agaacaagg agactgaaa agaggaagc 
      aacccgatt ctgagactg acaagccgc gtgaaaaac 
      agagaaaac tagataggt ttatgttta gtaaacgca 
      cgtgaatcg tgagtgatt tatttttat cg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY