Status
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Gene information
General information
TC012023 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7708157..7718274
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012023
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08949

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: 3 / 5 larvae on slide
    maxilla number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012023 RA
      MNHEPLRYA HEEGHTDVC FSTDGSKFI TCGADGDIR 
IWPSAEDTD PTHTCIGEW SLSVCQKGE CLYVATGSN 
DVQILTFPG GERNGLLDR FVAPINQIV PSKDSEAVA 
LAGEDMQVR VVELGAEKR VGVFDGLGG PCLCVTATS 
KFLAASSGD GKLRIWDVA SKELVKEIA CFPKTNSFA 
NAEYLCRID FAPNGKYLA YPENDSVVI LDTSDWSQY 
GILKTNEVT NHYSIVQFS PCGGFLLAT TLKGDFVIF 
NVSIKMVRG TSKHPNGTP ICGAVWNPS GNGQIVYTD 
TQGQLGIMS DCISIKTTD DEIFTNNDL NFDDVQFES 
DDEDNENAI SVEKLKKEV MGDVEPELN FDNASSAPT 
PRPKTPEIP LQAPFMPSS SPEHLNPRY LCWNHLGIV 
RCYGSTNDD DSGKSIEVE FLDSSFHHS MMLQNYQDY 
FLGTVSKAA LAVANTKQL YVIPLNAGS KEWVLKLEE 
DGTEEIVLV SASENLICF ATNSYIVRV CSIFGTQRG 
IVSVPGPLV SMASFQNSV LVAYHSGSV RNGDQCINI 
KLIAFNGLS MHSCDIGAA LGPESTLTW LGFTDFGTP 
AMVDSLGMV SLFPPNCNI WIPFCDTAR HMKSPSDCF 
FVTAVLESY QSVVGLKCR GTAYPSFTP TPTLSELIL 
EPPFAEPTT DKTQLEMSL FTLSNLQVS NQEKKFSEA 
GLKAFALAC KNNLDQRAV ELMEVLSNR QLVNLSTKY 
ALKLNKKIL AEKLMEMAG RLMNDDDDS TNVVVDTPT 
SSPLAPKKV TLSSLKRTP KTKQVVKEV VTAPEASQE 
SQSSILSPS FPIQEFSQE SPNPFSKSA RKGNESSNP 
LSLTDKYAG VDYVDAKEN KMKNKETEK RKQPDSETD 
KPREKQRKL DRFMFSKRT
    CDS sequence
    • >TC012023 RA
      atgaaccac gaaccctta cgctacgcc cacgaggag 
ggccacacg gacgtgtgc ttctcaacc gacggcagc 
aaatttatt acgtgtggg gctgacgga gacatccgg 
atctggccg tcagcggaa gacaccgac cccactcac 
acttgcata ggcgagtgg tcgctcagt gtgtgccag 
aaaggggag tgcttgtac gtggccacc ggcagcaat 
gacgtccaa atcctcacc tttccaggg ggcgaacgg 
aacgggctt ctggaccgg ttcgtggcc cccatcaac 
cagatcgtg cccagtaaa gacagcgag gctgtggcg 
cttgccggt gaagatatg caagtgagg gtggttgag 
ttgggggct gagaaaaga gttggggtt tttgatggg 
ttagggggg ccttgtttg tgtgtcacg gctactagt 
aagtttcta gcggcgagt tctggtgac ggaaaattg 
aggatttgg gatgttgcc agtaaggaa ctggttaaa 
gaaattgcc tgttttcct aaaactaac agttttgcc 
aacgctgaa tacttatgt cgtattgac tttgccccc 
aatggcaaa tatctggca tatccggaa aacgattca 
gttgttatt ttagacact tctgattgg tcacagtat 
ggtatttta aaaacaaat gaagtcacg aatcattat 
tcaattgta cagttttca ccctgtggc ggttttctt 
ctagcgaca actcttaaa ggtgacttt gtcattttt 
aatgttagc attaaaatg gtgagaggg actagtaaa 
caccctaat ggaacacca atttgtgga gccgtgtgg 
aacccctca ggaaacggc caaatcgtc tacaccgac 
actcaaggc cagttaggt attatgtca gattgtata 
agcatcaaa acaaccgac gacgaaatt ttcaccaat 
aacgaccta aacttcgac gacgtgcaa ttcgaatca 
gacgatgaa gacaacgaa aatgccatt tcggtcgaa 
aaactaaaa aaagaggtc atgggcgac gtagaaccc 
gagttaaat ttcgacaat gcatcttcg gcgcctact 
ccgcgcccc aaaaccccc gaaatcccg ctccaagcc 
ccatttatg cccagctcc agccctgag cacttgaat 
ccgcgctac ttgtgctgg aaccacctc ggaattgtg 
cgatgttac ggttccacg aacgacgac gattcggga 
aaatcaatc gaagttgag tttctcgat tcttcgttc 
catcacagc atgatgttg caaaactat caggactat 
ttcttgggg actgtcagt aaagcggcc ctcgctgtg 
gccaacaca aaacaactt tacgtcatt cccctgaat 
gcgggctca aaggagtgg gttcttaaa ttggaggaa 
gacggcacg gaagagatt gttttagtt tcagcttct 
gaaaatttg atttgtttc gcgacaaac agttacata 
gttagagtt tgctcgatt ttcggcact cagagagga 
atcgtttcg gttcctgga cctttagtt tccatggct 
agtttccag aattcggta cttgtggcc taccattcg 
ggaagtgtc cgcaacggt gaccagtgt attaacatc 
aaattgatt gcttttaat ggattgtcg atgcacagt 
tgcgatatc ggcgcggct ttaggcccc gagtccact 
ctgacgtgg ctgggattc accgatttt gggactcct 
gctatggtg gactcgctc ggaatggtc agtcttttt 
ccccccaat tgtaacatt tggatacct ttttgtgac 
actgccaga catatgaaa tcgccttct gactgtttt 
ttcgtgact gccgtcctt gaatcgtat cagtcggtt 
gttgggctc aagtgccgc ggcactgct tatccgagc 
ttcacccca acaccgaca ctcagtgaa ttgattttg 
gagccccca ttcgctgaa cccacaacg gataaaaca 
cagttggaa atgagtctt tttacgctt tcgaatttg 
caagtcagt aatcaggag aagaaattt tcagaggct 
gggttaaaa gcatttgcg ctagcatgt aagaacaat 
ctcgatcag agggcagtc gaacttatg gaagtttta 
tccaatcgt cagttggtg aatttgtca acgaaatac 
gcccttaaa ttgaataaa aaaatattg gcggagaaa 
ttgatggag atggctgga cgtctcatg aatgacgat 
gacgattca acgaatgtt gttgtggac acgccgaca 
tcttcaccc ctcgcccct aaaaaagtg acccttagt 
tcacttaaa cgtacgcca aaaaccaaa caagtcgtc 
aaagaagtg gtgacggca ccagaagcc agtcaagag 
agccaaagt tctatttta agcccgtct ttcccaatc 
caagaattt tcacaagaa tctccaaat ccattttcc 
aaaagtgca cgaaaaggg aacgaatca tcgaaccct 
ctgagtctc actgataaa tacgcgggt gttgattac 
gtcgatgct aaggaaaat aaaatgaag aacaaggag 
actgaaaag aggaagcaa cccgattct gagactgac 
aagccgcgt gaaaaacag agaaaacta gataggttt 
atgtttagt aaacgcacg tga
    mRNA sequence
    • >TC012023 RA
      tctagttat attccaaac accacattg gcgccactt 
cccgctttc gcgcctttt tccaacgtc aacaaaccc 
taacctgaa acattcaaa aaatgaacc acgaaccct 
tacgctacg cccacgagg agggccaca cggacgtgt 
gcttctcaa ccgacggca gcaaattta ttacgtgtg 
gggctgacg gagacatcc ggatctggc cgtcagcgg 
aagacaccg accccactc acacttgca taggcgagt 
ggtcgctca gtgtgtgcc agaaagggg agtgcttgt 
acgtggcca ccggcagca atgacgtcc aaatcctca 
cctttccag ggggcgaac ggaacgggc ttctggacc 
ggttcgtgg cccccatca accagatcg tgcccagta 
aagacagcg aggctgtgg cgcttgccg gtgaagata 
tgcaagtga gggtggttg agttggggg ctgagaaaa 
gagttgggg tttttgatg ggttagggg ggccttgtt 
tgtgtgtca cggctacta gtaagtttc tagcggcga 
gttctggtg acggaaaat tgaggattt gggatgttg 
ccagtaagg aactggtta aagaaattg cctgttttc 
ctaaaacta acagttttg ccaacgctg aatacttat 
gtcgtattg actttgccc ccaatggca aatatctgg 
catatccgg aaaacgatt cagttgtta ttttagaca 
cttctgatt ggtcacagt atggtattt taaaaacaa 
atgaagtca cgaatcatt attcaattg tacagtttt 
caccctgtg gcggttttc ttctagcga caactctta 
aaggtgact ttgtcattt ttaatgtta gcattaaaa 
tggtgagag ggactagta aacacccta atggaacac 
caatttgtg gagccgtgt ggaacccct caggaaacg 
gccaaatcg tctacaccg acactcaag gccagttag 
gtattatgt cagattgta taagcatca aaacaaccg 
acgacgaaa ttttcacca ataacgacc taaacttcg 
acgacgtgc aattcgaat cagacgatg aagacaacg 
aaaatgcca tttcggtcg aaaaactaa aaaaagagg 
tcatgggcg acgtagaac ccgagttaa atttcgaca 
atgcatctt cggcgccta ctccgcgcc ccaaaaccc 
ccgaaatcc cgctccaag ccccattta tgcccagct 
ccagccctg agcacttga atccgcgct acttgtgct 
ggaaccacc tcggaattg tgcgatgtt acggttcca 
cgaacgacg acgattcgg gaaaatcaa tcgaagttg 
agtttctcg attcttcgt tccatcaca gcatgatgt 
tgcaaaact atcaggact atttcttgg ggactgtca 
gtaaagcgg ccctcgctg tggccaaca caaaacaac 
tttacgtca ttcccctga atgcgggct caaaggagt 
gggttctta aattggagg aagacggca cggaagaga 
ttgttttag tttcagctt ctgaaaatt tgatttgtt 
tcgcgacaa acagttaca tagttagag tttgctcga 
ttttcggca ctcagagag gaatcgttt cggttcctg 
gacctttag tttccatgg ctagtttcc agaattcgg 
tacttgtgg cctaccatt cgggaagtg tccgcaacg 
gtgaccagt gtattaaca tcaaattga ttgctttta 
atggattgt cgatgcaca gttgcgata tcggcgcgg 
ctttaggcc ccgagtcca ctctgacgt ggctgggat 
tcaccgatt ttgggactc ctgctatgg tggactcgc 
tcggaatgg tcagtcttt ttcccccca attgtaaca 
tttggatac ctttttgtg acactgcca gacatatga 
aatcgcctt ctgactgtt ttttcgtga ctgccgtcc 
ttgaatcgt atcagtcgg ttgttgggc tcaagtgcc 
gcggcactg cttatccga gcttcaccc caacaccga 
cactcagtg aattgattt tggagcccc cattcgctg 
aacccacaa cggataaaa cacagttgg aaatgagtc 
tttttacgc tttcgaatt tgcaagtca gtaatcagg 
agaagaaat tttcagagg ctgggttaa aagcatttg 
cgctagcat gtaagaaca atctcgatc agagggcag 
tcgaactta tggaagttt tatccaatc gtcagttgg 
tgaatttgt caacgaaat acgccctta aattgaata 
aaaaaatat tggcggaga aattgatgg agatggctg 
gacgtctca tgaatgacg atgacgatt caacgaatg 
ttgttgtgg acacgccga catcttcac ccctcgccc 
ctaaaaaag tgaccctta gttcactta aacgtacgc 
caaaaacca aacaagtcg tcaaagaag tggtgacgg 
caccagaag ccagtcaag agagccaaa gttctattt 
taagcccgt ctttcccaa tccaagaat tttcacaag 
aatctccaa atccatttt ccaaaagtg cacgaaaag 
ggaacgaat catcgaacc ctctgagtc tcactgata 
aatacgcgg gtgttgatt acgtcgatg ctaaggaaa 
ataaaatga agaacaagg agactgaaa agaggaagc 
aacccgatt ctgagactg acaagccgc gtgaaaaac 
agagaaaac tagataggt ttatgttta gtaaacgca 
cgtgaatcg tgagtgatt tatttttat cg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY