Status
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Gene information
General information
TC001699 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7758828..7760536
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001699
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late structure degenerated - Number of animals showing the phenotype: 4

    yolk late deposition irregular - Number of animals showing the phenotype: 4

    previtellogenic egg chamber sometimes potentiallynumber increased - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001699 RA
      MALELEVVK TSGKPVGKV SLASSATVK DLKKEIFKN 
      FRLGVERQS IRNGPKGKD EKDSQEVGK IHLETAGTI 
      YVKDLGPQI GWDTVFFLE YAGPIFLYG ALFLFPHCF 
      YGGKSLEQG RGQNARNIF PFYVFPVEQ INKICGGSL 
      IKVQDNAYY AGILWTIHY GKRILETAF VHRFSHGTM 
      PIFNLFKNC SYYWGFAIY LAYHVNHPL HTAPPKWLF 
      HLGVFIFIF CELGNLSIH ILLRNLRPE GSTVRKIPV 
      PDSNPFTKL FNYVSCPNY TYEFGSWVG FTLLTGCFP 
      VFIFALAGL YQMTVWAIG KHKKYRQEF PNYPKKRKA 
      ILPFLI
    CDS sequence
    • >TC001699 RA
      atggctctc gagttggaa gtggtgaaa acgtccgga 
      aaaccggtc gggaaagtc tcactggcg agttcggcg 
      acggtgaag gacttgaaa aaggaaatt ttcaaaaac 
      tttaggctg ggggtcgag cgccagtcc attcggaac 
      gggccgaag ggaaaagac gagaaagac agccaggaa 
      gttggcaaa atccactta gagaccgcc ggcacgatt 
      tacgttaag gatttgggg ccgcaaatc gggtgggac 
      accgtcttc ttcttggaa tacgcgggc cccattttc 
      ctgtacggc gccctgttc ctgttcccg cactgcttc 
      tacgggggc aagagcctg gagcagggc cgggggcaa 
      aacgcgcgg aatatcttc ccgttttat gtttttccg 
      gtcgagcaa attaacaaa atatgcggc ggctcactt 
      atcaaagtc caagataat gcatactat gctggtatt 
      ttatggact attcactac gggaaacga atccttgaa 
      acggcgttc gtgcaccga ttttcccac gggacgatg 
      ccgattttc aacctgttt aagaactgc tcgtactat 
      tggggcttc gccatttat ctagcctac catgtcaac 
      caccccttg cacacggcg cccccgaag tggctgttc 
      cacctcggc gttttcatt tttatcttc tgcgaactc 
      ggcaatttg agtatccac attttgttg cgaaatttg 
      cggcccgag ggctcgacc gtgcggaaa attccagta 
      ccggactca aacccgttc acgaaatta ttcaattac 
      gtctcatgt cctaattac acctacgag tttgggtct 
      tgggtcggt ttcacgctc ttgacgggc tgttttcca 
      gtttttatt tttgcgctc gcgggtctc taccagatg 
      accgtgtgg gccatcggc aagcacaaa aagtacagg 
      caggagttc ccgaattat cccaagaag aggaaggcg 
      attttgcca ttcctgatt taa
    mRNA sequence
    • >TC001699 RA
      taaaaaagg gtttacggg gccggccgg tcgtttttc 
      gaccacgtg actgataag tcgacgaaa ccgaaacaa 
      tcaattttt aattaaatg caaaaccat tgtccgact 
      tcccacttg ccccattgc ttattaagc attcgacgg 
      ttcagtgtc tctgtttaa ttggtggaa ttttggaat 
      ccatcctcg tttaattag ttgtttatt cggtggcca 
      ggtatcctt gaagttcac attccggcg gcgtcgaag 
      aaaattcaa cagcgtcat ggctctcga gttggaagt 
      ggtgaaaac gtccggaaa accggtcgg gaaagtctc 
      actggcgag ttcggcgac ggtgaagga cttgaaaaa 
      ggaaatttt caaaaactt taggctggg ggtcgagcg 
      ccagtccat tcggaacgg gccgaaggg aaaagacga 
      gaaagacag ccaggaagt tggcaaaat ccacttaga 
      gaccgccgg cacgattta cgttaagga tttggggcc 
      gcaaatcgg gtgggacac cgtcttctt cttggaata 
      cgcgggccc cattttcct gtacggcgc cctgttcct 
      gttcccgca ctgcttcta cgggggcaa gagcctgga 
      gcagggccg ggggcaaaa cgcgcggaa tatcttccc 
      gttttatgt ttttccggt cgagcaaat taacaaaat 
      atgcggcgg ctcacttat caaagtcca agataatgc 
      atactatgc tggtatttt atggactat tcactacgg 
      gaaacgaat ccttgaaac ggcgttcgt gcaccgatt 
      ttcccacgg gacgatgcc gattttcaa cctgtttaa 
      gaactgctc gtactattg gggcttcgc catttatct 
      agcctacca tgtcaacca ccccttgca cacggcgcc 
      cccgaagtg gctgttcca cctcggcgt tttcatttt 
      tatcttctg cgaactcgg caatttgag tatccacat 
      tttgttgcg aaatttgcg gcccgaggg ctcgaccgt 
      gcggaaaat tccagtacc ggactcaaa cccgttcac 
      gaaattatt caattacgt ctcatgtcc taattacac 
      ctacgagtt tgggtcttg ggtcggttt cacgctctt 
      gacgggctg ttttccagt ttttatttt tgcgctcgc 
      gggtctcta ccagatgac cgtgtgggc catcggcaa 
      gcacaaaaa gtacaggca ggagttccc gaattatcc 
      caagaagag gaaggcgat tttgccatt cctgattta 
      atttccacg gttgggttc ttgtttcct attttgttg 
      tttttttaa gtggtgttt gcgtaataa aatggtttg 
      atggatcga gttttattc aataaataa aggtacaaa 
      tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY