Status
Please wait ...
Gene information
General information
TC006725 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13384359..13392036
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006725
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006725 RA
      MPPIKDLLL LESATSIAH KIRTKKITS LQVLESFIA 
      RIHEVNPIL NCVVAERFE EARKEARAV DDLIKSGAI 
      PEETLAREK PFLGVPFTT KDCIAVKGM IHTSGLAKR 
      RNCIAEEDA DAIACLKSA GAFPIALTN VSELCMWWE 
      SANTIHGRS NNPYDTNHI VGGSSGGEG CLQGAAGSA 
      FGIGSDIGG SIRMPSFFN GIFGHKPSK FIVSNNGQY 
      PAPITTEQT SFLGIGPMC RRAEDLLPL LKIIAGKNA 
      NELKLDEPV DVKKLKFYY QETDGGSVG VSPVNHEIR 
      QLFTKIALH LEKAHAIKA KKVALERFR KSAPIWFAN 
      MKSPNGPSF QEQLANLQG SINPYWELV KWVFRRSDH 
      TFIGIVTAL ADKGGCKYG DDKHTYLVE ERGRLRREM 
      EELLGDDGV FLYPTHPTA APFHNEPLI KPFNFSYTA 
      IINVLGFPA TNIPMGLNG EGLPIGVQV VANTKNDRL 
      CLAVARELE KAFGGWVPP EVDA
    CDS sequence
    • >TC006725 RA
      atgccaccg attaaagac ctcctattg ctggaatcg 
      gccacttcc atagcgcac aaaatcagg accaaaaaa 
      atcacaagc ctccaagtg ctcgagtcg ttcatcgct 
      cgcatacac gaagtgaat ccaatttta aattgcgtg 
      gtggccgag cggtttgag gaggcgcgc aaggaggct 
      cgggctgtt gatgacctg atcaagtcg ggggcgatc 
      cccgaggag accctcgcg agggagaag cccttcctg 
      ggggtgccc ttcacgacc aaagactgc atcgcagta 
      aagggaatg attcacact tccggattg gccaagcgg 
      cggaattgc attgctgag gaagatgcc gatgctatt 
      gcctgtctc aagagcgct ggggctttt cccattgcc 
      ttgaccaac gtttcggag ctttgcatg tggtgggag 
      agcgccaac acgatccac ggccgctcg aacaacccc 
      tacgacacc aaccacatc gtggggggc tcgtcgggg 
      ggcgaaggc tgcctccag ggggctgcc ggttcagcc 
      ttcggcatc ggctccgac atcggaggc tcaatccgc 
      atgccttcg tttttcaac ggaattttc ggccacaaa 
      ccctccaaa ttcatagtc tcaaacaac ggccagtac 
      ccagccccc ataaccacc gaacagacc agtttcctg 
      ggcattggc cccatgtgt cgccgggct gaggacctc 
      ctccccctc ctcaaaata atcgccggg aaaaacgca 
      aacgaactc aaactggac gaaccagtt gatgtgaaa 
      aaattgaaa ttttactat caggagacg gatgggggc 
      tcagtgggc gtctcgccc gttaatcac gagattcgg 
      caacttttc acgaagatt gcgctgcat ttggagaag 
      gcacatgcc attaaggcc aagaaagtc gcgcttgag 
      cgcttcagg aaaagtgcc cccatttgg ttcgccaat 
      atgaagagc cccaacggg ccctcgttc caggagcag 
      ttggctaat ttgcaaggg tcgatcaac ccgtattgg 
      gagttggtt aagtgggtt tttcgccgg tcggatcac 
      acgtttatc ggcattgtc acggctttg gccgataaa 
      gggggctgc aagtacggg gatgataag cacacttat 
      ctggtggag gagcgcggg aggctgcgt cgggagatg 
      gaggagctc ctgggggat gacggggtg ttcctgtac 
      cccacgcat cccacggca gcccccttc cacaacgag 
      cccctgatc aagccgttc aatttcagc tacactgcc 
      atcattaat gttttgggg ttcccggcg accaacatc 
      cccatgggg ctgaatggg gaggggctg cccatcggg 
      gttcaagtg gtggcgaat acgaaaaat gatagattg 
      tgtctggcg gtggctagg gagttggag aaggcgttc 
      gggggctgg gtgccccct gaagtggac gcttaa
    mRNA sequence
    • >TC006725 RA
      tatttccgc cgaataaaa ccggctatc tttttgccc 
      gctgcacca caaaataat caaatttac ctgttgccg 
      atcccccgg tgctgacgg aggcgacgc ctgctgcca 
      gcttgccaa catcgcgca aaacggggg gcaccggtc 
      tttgagact ccacacttt tcgaactgc cgggcttcg 
      aactcatgg agcccgcaa tcaccacat gaactcgaa 
      cggtcacac tcgcgtttt tggccgatt ttttcgcaa 
      aaaatcggc cggtttgac agatgacgt aacctcact 
      ttttgtaaa cttgttgtt ttttcagtt attgggggt 
      tgcaagggt gggtttttt gtcacgggt caacatttc 
      gattttgtg attaattat tcaaattgt cgcacgcgt 
      ttgggggtt tttaacgat tttgaggcg cacttgagc 
      taacctcac tttgcctta accacaatt tattccagt 
      cgatgacag cgaagtggg ggtatcatc aatttacgc 
      gtagcagga aaaattagt tgtcttgtg gtgtgtcgt 
      gtgatgagt gtgtagttt tttaaataa gtaatcatg 
      ctgcaattc gggaaatct gttataagg atactgcat 
      gggctggtg gaatgtttc tttcattgt gttttcaag 
      ctagtttat tgtggctct ggccagaaa atgccaccg 
      attaaagac ctcctattg ctggaatcg gccacttcc 
      atagcgcac aaaatcagg accaaaaaa atcacaagc 
      ctccaagtg ctcgagtcg ttcatcgct cgcatacac 
      gaagtgaat ccaatttta aattgcgtg gtggccgag 
      cggtttgag gaggcgcgc aaggaggct cgggctgtt 
      gatgacctg atcaagtcg ggggcgatc cccgaggag 
      accctcgcg agggagaag cccttcctg ggggtgccc 
      ttcacgacc aaagactgc atcgcagta aagggaatg 
      attcacact tccggattg gccaagcgg cggaattgc 
      attgctgag gaagatgcc gatgctatt gcctgtctc 
      aagagcgct ggggctttt cccattgcc ttgaccaac 
      gtttcggag ctttgcatg tggtgggag agcgccaac 
      acgatccac ggccgctcg aacaacccc tacgacacc 
      aaccacatc gtggggggc tcgtcgggg ggcgaaggc 
      tgcctccag ggggctgcc ggttcagcc ttcggcatc 
      ggctccgac atcggaggc tcaatccgc atgccttcg 
      tttttcaac ggaattttc ggccacaaa ccctccaaa 
      ttcatagtc tcaaacaac ggccagtac ccagccccc 
      ataaccacc gaacagacc agtttcctg ggcattggc 
      cccatgtgt cgccgggct gaggacctc ctccccctc 
      ctcaaaata atcgccggg aaaaacgca aacgaactc 
      aaactggac gaaccagtt gatgtgaaa aaattgaaa 
      ttttactat caggagacg gatgggggc tcagtgggc 
      gtctcgccc gttaatcac gagattcgg caacttttc 
      acgaagatt gcgctgcat ttggagaag gcacatgcc 
      attaaggcc aagaaagtc gcgcttgag cgcttcagg 
      aaaagtgcc cccatttgg ttcgccaat atgaagagc 
      cccaacggg ccctcgttc caggagcag ttggctaat 
      ttgcaaggg tcgatcaac ccgtattgg gagttggtt 
      aagtgggtt tttcgccgg tcggatcac acgtttatc 
      ggcattgtc acggctttg gccgataaa gggggctgc 
      aagtacggg gatgataag cacacttat ctggtggag 
      gagcgcggg aggctgcgt cgggagatg gaggagctc 
      ctgggggat gacggggtg ttcctgtac cccacgcat 
      cccacggca gcccccttc cacaacgag cccctgatc 
      aagccgttc aatttcagc tacactgcc atcattaat 
      gttttgggg ttcccggcg accaacatc cccatgggg 
      ctgaatggg gaggggctg cccatcggg gttcaagtg 
      gtggcgaat acgaaaaat gatagattg tgtctggcg 
      gtggctagg gagttggag aaggcgttc gggggctgg 
      gtgccccct gaagtggac gcttaaatg ccttagtga 
      tagcacagt attcggact gagagagcc aagctcaaa 
      gtaccaacc cttttatag aactaccta tattttata 
      aatgtttac acggttgat gtgtctgta tcacgttct 
      attttaatc atttgtact atttacaat gagattatt 
      tttataact caatgttga attttataa taaaatctc 
      tctcctgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY