Status
Please wait ...
Gene information
General information
TC006697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13054237..13059031
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006697
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08943

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 2

    mature egg shape irregular - Number of animals showing the phenotype: 1
    tropharium potentially structure degenerated - Number of animals showing the phenotype: 1

    egg chamber not present - Number of animals showing the phenotype: 1

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    7

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006697 RA
      MNHLSLVTL FCLFGAIFA QDLPSCESK IYCQGKLLD 
      TIQMARLFD DSKTFVDMA LKNPAETVL ENFDKLLQD 
      TDNNPSKDD LEQFLEENF ENREELEEW SPEDYTESP 
      KFLEKIQDE SLKQFAQEL VKLWPKFGR KMSPEVGQN 
      PTKHSLIPV TNGFIVPGG RFNEFYYWD TYWIIKGLL 
      MCEMKDTVK GILENFYQI VKKYGFVPN GARVYYLNR 
      SQPPLLALM TAAYFKESN DSQWLGEQI PVLETELNF 
      WLQNRSIEV NLGENSYTL FHYASESGS PRPESYYED 
      VQSANKIEN EAQKVQLWA DLKSAAESG WDFSSRWMS 
      DPNGDLTDA QTRNIIPVD LNSFLCQAF SIMSSFHQT 
      LGNDQEASL WATKTQTLK SAIKEVFFD ETDGVWYDY 
      DLKLAQKRK NFYPSNLTP LWARASHEI DAGKIVQYL 
      GQVLGYGGG VPASLTKSG QQWDFPGAW PPLQELVIN 
      SLYRTKDSK AIEVAKELS QKWLKSNVQ AFDKYGAMF 
      EKYDAERVG EPGGGGEYE VQEGFGWTN GVALMLIKW 
      A
    CDS sequence
    • >TC006697 RA
      atgaaccac ttgtccctt gtcacactt ttttgccta 
      tttggggcc attttcgcc caagattta ccgtcatgt 
      gagagcaaa atttactgc caaggcaaa ttgctcgac 
      acgatccaa atggcccga cttttcgac gattccaaa 
      actttcgtc gacatggcc ttgaaaaac cccgcagaa 
      acggtactc gaaaatttc gataagttg ctccaggac 
      actgacaat aacccgagc aaggacgac ttggaacag 
      ttccttgag gaaaatttc gaaaatcgg gaggaactc 
      gaagagtgg agtccggag gactacacg gaaagtcca 
      aaatttttg gaaaaaatc caagacgag agtttgaaa 
      caattcgcc caggagttg gtcaagctt tggccgaaa 
      ttcggccgg aagatgagc ccagaagtt gggcaaaac 
      ccaaccaaa cactcactc attccggtc accaacgga 
      tttattgtt ccaggaggg cgttttaac gaattttac 
      tactgggac acttattgg attattaag ggacttttg 
      atgtgcgaa atgaaggat acggtcaag ggaattttg 
      gaaaacttc taccaaatt gtaaaaaaa tatggtttt 
      gtgccaaac ggtgccagg gtttactac ttgaaccgg 
      tcccaaccc ccactcctc gcactgatg acagctgct 
      tattttaag gaatcaaac gactcgcaa tggctgggt 
      gaacaaatc cctgttctg gaaaccgag ctgaatttt 
      tggctccaa aaccgttcg attgaagtc aatttgggc 
      gaaaacagc tacactcta ttccactac gcctccgaa 
      agtggaagt ccgagacca gagtcctac tacgaggac 
      gtccaaagt gctaacaaa atcgaaaat gaagcccaa 
      aaagtccag ttatgggcc gatttgaaa agcgcggct 
      gaaagtggc tgggacttc tcctcccgc tggatgtcc 
      gaccccaac ggcgacctc accgacgcc caaaccagg 
      aacataatc cccgtagac ttaaactcc ttcctctgc 
      caagctttc agcatcatg tcctccttt caccaaact 
      ttgggcaac gaccaggaa gcctctctc tgggccacc 
      aaaacccaa actttaaaa tcggccata aaagaggtt 
      tttttcgac gaaaccgac ggagtttgg tacgattac 
      gacctgaaa ttagcccag aagcggaaa aacttctac 
      cccagtaat ttgacccct ttgtgggcg cgcgcgagc 
      cacgaaatc gacgcgggg aaaatcgtg cagtatctg 
      gggcaggtt ttggggtac gggggcggc gtgcctgcg 
      tccttgacc aagtcgggg cagcagtgg gacttcccg 
      ggggcttgg ccccctttg caggaactt gtcataaat 
      tcgctctac cgtacgaag gattccaaa gcgattgag 
      gtggctaag gagttgtcg caaaaatgg ctcaagtct 
      aatgtccag gcgttcgat aaatatggg gccatgttt 
      gagaagtat gacgctgaa agagtgggg gagcccggg 
      ggtgggggc gagtacgag gtccaggag gggtttggg 
      tggaccaat ggggtggcc ttgatgcta atcaagtgg 
      gcttga
    mRNA sequence
    • >TC006697 RA
      tggtcacag tgtcagttg caatttccg caaatcagc 
      acagtgtcg actgctgaa agaattttc gaaaaagtg 
      cgctgttgc aacaagtgg aaataaaac cgcaattga 
      aagaaattg tcagtggtt cctgtctta gtggatgct 
      caaatcacc caacaatag cttgcgttc aattcccga 
      gctactatc gcaatgaac cacttgtcc cttgtcaca 
      cttttttgc ctatttggg gccattttc gcccaagat 
      ttaccgtca tgtgagagc aaaatttac tgccaaggc 
      aaattgctc gacacgatc caaatggcc cgacttttc 
      gacgattcc aaaactttc gtcgacatg gccttgaaa 
      aaccccgca gaaacggta ctcgaaaat ttcgataag 
      ttgctccag gacactgac aataacccg agcaaggac 
      gacttggaa cagttcctt gaggaaaat ttcgaaaat 
      cgggaggaa ctcgaagag tggagtccg gaggactac 
      acggaaagt ccaaaattt ttggaaaaa atccaagac 
      gagagtttg aaacaattc gcccaggag ttggtcaag 
      ctttggccg aaattcggc cggaagatg agcccagaa 
      gttgggcaa aacccaacc aaacactca ctcattccg 
      gtcaccaac ggatttatt gttccagga gggcgtttt 
      aacgaattt tactactgg gacacttat tggattatt 
      aagggactt ttgatgtgc gaaatgaag gatacggtc 
      aagggaatt ttggaaaac ttctaccaa attgtaaaa 
      aaatatggt tttgtgcca aacggtgcc agggtttac 
      tacttgaac cggtcccaa cccccactc ctcgcactg 
      atgacagct gcttatttt aaggaatca aacgactcg 
      caatggctg ggtgaacaa atccctgtt ctggaaacc 
      gagctgaat ttttggctc caaaaccgt tcgattgaa 
      gtcaatttg ggcgaaaac agctacact ctattccac 
      tacgcctcc gaaagtgga agtccgaga ccagagtcc 
      tactacgag gacgtccaa agtgctaac aaaatcgaa 
      aatgaagcc caaaaagtc cagttatgg gccgatttg 
      aaaagcgcg gctgaaagt ggctgggac ttctcctcc 
      cgctggatg tccgacccc aacggcgac ctcaccgac 
      gcccaaacc aggaacata atccccgta gacttaaac 
      tccttcctc tgccaagct ttcagcatc atgtcctcc 
      tttcaccaa actttgggc aacgaccag gaagcctct 
      ctctgggcc accaaaacc caaacttta aaatcggcc 
      ataaaagag gtttttttc gacgaaacc gacggagtt 
      tggtacgat tacgacctg aaattagcc cagaagcgg 
      aaaaacttc taccccagt aatttgacc cctttgtgg 
      gcgcgcgcg agccacgaa atcgacgcg gggaaaatc 
      gtgcagtat ctggggcag gttttgggg tacgggggc 
      ggcgtgcct gcgtccttg accaagtcg gggcagcag 
      tgggacttc ccgggggct tggccccct ttgcaggaa 
      cttgtcata aattcgctc taccgtacg aaggattcc 
      aaagcgatt gaggtggct aaggagttg tcgcaaaaa 
      tggctcaag tctaatgtc caggcgttc gataaatat 
      ggggccatg tttgagaag tatgacgct gaaagagtg 
      ggggagccc gggggtggg ggcgagtac gaggtccag 
      gaggggttt gggtggacc aatggggtg gccttgatg 
      ctaatcaag tgggcttga ataaatcgt tgtgaattt 
      ttttaccta attttgaaa aaaaaaacg atatggttg 
      taatttaac tcgtatctt ttattatgt atcacaggc 
      ggtgataac attttaaac ttccgtccg tttgcggta 
      taaatccaa aaaaatcga acaaactca agtagataa 
      gtgaaagat tttggaaaa gtttgtagt atcggtgac 
      ccgaaaccg ataattaga gtgaaattt tcgggtgat 
      catcacact ttgaaatga tgtacacgt gtgtttgat 
      tcgcaatca taaaatcac actgacaaa tctttgttt 
      gagtgaaac acttttctc ggtaagtct ccgttgttt 
      gttgtcaag tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY