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Gene information
General information
TC004626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6302970..6306844
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004626
    iBeetle screen iB_08941

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004626 RA
      MGVSYDLFF LTFLFLLTC TFGYFGNVL DDDCFCQLH 
      GKIDDCSCN VDTVDHFNN VKIYPRLRS LLHKGYFRF 
      YKVNLRRPC PFWSDDSRC AMRYCHVEA CEDKDIPVG 
      LKGGLENHS SKNLYVTQS CEDDHNAEL GYLNTTISA 
      KVQEDIVLW SAYDDAQDN FCVLEDNDS ESEYVDLLL 
      NPERYTGYR GKSAHRIWN SIYMENCFR STKNDNPYI 
      QSSKLNNMC LEERVFYRA ISGLHASIN IHLCAQFLL 
      SETSLSEPD GKWGPNLSE FLRRFSKET TRGEGPHWL 
      RNLYFVYLL ELRALNKAM RYLEKEEFY TGNESEDWD 
      TQLAVKDLL SVVQKFPNH FNETTMFTG SEQAEKLKY 
      EFKQHFRNV SKIMDCVGC DKCRLWGKL QVQGLGTAL 
      KILFSGKFD DQLDLSSDR KKFQLQRSE IVSLVNSIG 
      RLSTSIYEL DEFRQMLR
    CDS sequence
    • >TC004626 RA
      atgggggtt agctacgat ttgtttttt ctcacgttt 
      ttgttcctt ttgacttgc acgttcggg tactttggc 
      aacgtcttg gacgatgat tgtttttgc cagcttcat 
      ggaaaaatc gacgactgt tcgtgtaac gtagataca 
      gtagaccac tttaataac gtaaaaatc taccccaga 
      ttgcgcagt ttgctccac aaggggtat ttccgcttt 
      tataaggtg aatttgcgg agaccatgt cccttttgg 
      tctgacgac agtagatgt gccatgagg tactgtcac 
      gtggaggct tgcgaagat aaagacatc cctgtgggg 
      ctcaaaggc ggtttagaa aaccacagt tcaaaaaat 
      ttgtatgtg actcaaagt tgtgaagac gatcacaac 
      gccgagctg ggttacttg aatactaca attagcgca 
      aaagtgcaa gaagacata gttctctgg agcgcttac 
      gacgacgcc caagacaac ttttgcgtc cttgaggac 
      aacgacagt gaatcggaa tacgtggat ttgcttcta 
      aatccagaa cgatacact ggatacagg ggcaagtcg 
      gcgcaccgt atttggaac agcatctac atggagaat 
      tgcttcaga agcacgaaa aacgacaat ccgtacatc 
      cagagctcc aagctcaac aatatgtgt ctggaggaa 
      cgcgttttt taccgagct atttcgggc ctccacgct 
      agcataaat atccacttg tgcgcgcaa tttttgctc 
      tccgaaacg agtttgagc gaacctgac ggcaagtgg 
      gggcccaat ttgagcgag tttttgaga cgcttctcg 
      aaagagacc acgcgcggt gaaggcccc cactggctc 
      cggaattta tattttgtt tacttgctc gagctgcga 
      gcgttaaat aaagccatg cgttatttg gagaaagag 
      gagttttac acggggaat gagagcgaa gactgggac 
      acgcaactt gctgttaag gacctcctc agtgtcgtc 
      caaaaattt ccaaaccac ttcaacgaa accaccatg 
      ttcacgggc agcgaacag gcggagaag cttaagtac 
      gaatttaag caacacttc cggaatgtg tccaaaatt 
      atggactgt gtcgggtgc gataaatgc agattgtgg 
      ggaaagtta caagtgcag ggcttagga actgcgttg 
      aaaattctt ttttccggg aagtttgat gatcagctg 
      gacttatca tcggacagg aagaaattc cagttgcaa 
      agaagtgag attgtctca cttgtaaat agtatcgga 
      aggctgtct acgagtatt tatgaattg gacgaattc 
      cgtcaaatg ttacgatga
    mRNA sequence
    • >TC004626 RA
      attacctaa taaatgaat tttccgcaa ataatgccc 
      agtttttta ggttacaat tttttgaca gcttttgtc 
      aaactgtcg ataaaagcg cgcgcattc attggtgtg 
      atatttcac ttgaaaatt atttttcca acaataata 
      acaatataa ttcaaagaa aaaacattt tttacattt 
      ataaaatta tcaaagacg acgttaaat cacacaatg 
      cgtgaagat aacacgttt gctcgtagt tcggtcttg 
      aggttagat gtcaactgc ctgccaaag tgtttgtca 
      gtgttatca cttatcaga aaatgtggc agttttcaa 
      aacaatttc aaatgagtg ttagtgtgt cactgtgga 
      aacgtgata gtcgtaact taaaaaatc gtaattgat 
      atgttgttg aaacgcctc cctgtaacc tagtgacga 
      tgggggtta gctacgatt tgttttttc tcacgtttt 
      tgttccttt tgacttgca cgttcgggt actttggca 
      acgtcttgg acgatgatt gtttttgcc agcttcatg 
      gaaaaatcg acgactgtt cgtgtaacg tagatacag 
      tagaccact ttaataacg taaaaatct accccagat 
      tgcgcagtt tgctccaca aggggtatt tccgctttt 
      ataaggtga atttgcgga gaccatgtc ccttttggt 
      ctgacgaca gtagatgtg ccatgaggt actgtcacg 
      tggaggctt gcgaagata aagacatcc ctgtggggc 
      tcaaaggcg gtttagaaa accacagtt caaaaaatt 
      tgtatgtga ctcaaagtt gtgaagacg atcacaacg 
      ccgagctgg gttacttga atactacaa ttagcgcaa 
      aagtgcaag aagacatag ttctctgga gcgcttacg 
      acgacgccc aagacaact tttgcgtcc ttgaggaca 
      acgacagtg aatcggaat acgtggatt tgcttctaa 
      atccagaac gatacactg gatacaggg gcaagtcgg 
      cgcaccgta tttggaaca gcatctaca tggagaatt 
      gcttcagaa gcacgaaaa acgacaatc cgtacatcc 
      agagctcca agctcaaca atatgtgtc tggaggaac 
      gcgtttttt accgagcta tttcgggcc tccacgcta 
      gcataaata tccacttgt gcgcgcaat ttttgctct 
      ccgaaacga gtttgagcg aacctgacg gcaagtggg 
      ggcccaatt tgagcgagt ttttgagac gcttctcga 
      aagagacca cgcgcggtg aaggccccc actggctcc 
      ggaatttat attttgttt acttgctcg agctgcgag 
      cgttaaata aagccatgc gttatttgg agaaagagg 
      agttttaca cggggaatg agagcgaag actgggaca 
      cgcaacttg ctgttaagg acctcctca gtgtcgtcc 
      aaaaatttc caaaccact tcaacgaaa ccaccatgt 
      tcacgggca gcgaacagg cggagaagc ttaagtacg 
      aatttaagc aacacttcc ggaatgtgt ccaaaatta 
      tggactgtg tcgggtgcg ataaatgca gattgtggg 
      gaaagttac aagtgcagg gcttaggaa ctgcgttga 
      aaattcttt tttccggga agtttgatg atcagctgg 
      acttatcat cggacagga agaaattcc agttgcaaa 
      gaagtgaga ttgtctcac ttgtaaata gtatcggaa 
      ggctgtcta cgagtattt atgaattgg acgaattcc 
      gtcaaatgt tacgatgac cttggtggt cgtcaagtg 
      gcaaaaccg tttcataat ttaacaaag agcggtcgt 
      gggaccaaa gtgtgcaga ggcgtgcta gaatggtaa 
      cgaaaatat gtatagaat tagttcggt gatgtgtaa 
      ctccgccct tacccaaaa tagtaacca aactacatt 
      ttgaattaa tttggcagt gaactgcta gaaagttaa 
      aaatggtta acgaagtga cggtagcaa aattcgtga 
      acaggggcg tggcaaagt ggtaacgaa aataagttt 
      agaattagt ttgatgatg ataaactgc tcgtttggg 
      aaaaaatat acatttact gcatttatc aacaaaaag 
      aaagttgaa ttatgaatt attacttaa ttaactcag 
      tcgtggaca ggagcgtgc tagtggtaa cgaaaatac 
      gtttagacg tggtatgtg gagaccccg ttttaattg 
      aatgtctgc ttccaaaat tacctccaa aattgtagg 
      gtggtaacg aaaatatgt ttagacttt tttcggtaa 
      tgaaatggc caaaaaaaa tcgtaatcg acaaaatga 
      aattgctat ttaggcgtg gtaatgtgg tcacgaaaa 
      tacgtttag acgtgatat ctagcggcc acgctccta 
      ttgctgcga caaaattat tttagaatc gtagtggta 
      acgaaaata tgttcagac ttatttcga tgaagaaat 
      gggcaaaaa tttaaaaaa gtagtcaac aaaatgaag 
      ttacatttt tgattcacc taattaata aggcgtggt 
      aacgaaaat acgtttaga attagttcg gggataata 
      caggcgtgg ctgtgaact gaccacgcc tttaaacca 
      ccatgacgt gaagatatg cgagactta tttacataa 
      tttggcaaa aaaccgtaa aaatgtaag cagtagtgc 
      gtaacagtg tggtcacga aaatacgtt tagacgtga 
      tatctagcg gccacgctt tttttgctg cgacaaaat 
      tattttaga atcgtagtg gtaacgaaa atatgttca 
      gacttattt cgatgaagg aatgggcaa aaatttaaa 
      aaagtagtc aacaaaatg aagttacaa tattgattc 
      acctaatta ataaggcgt ggtaacgaa aatatgttc 
      agacttatt tagatgaag aaatgggca aaaatttca 
      aaaagtaaa atgtaatca gtagtgcat aacagtgtg 
      gtaacgaaa aaacgttta gacgtgata tctagcggc 
      cacgcccct gatgctgcg acaaaattg gtaacgaaa 
      atatgttca gaattagtt cggtgatgt gtaactccg 
      cccttaccc aaaacagta accaaacta tatttttaa 
      ttaatttgg cagtgaact gctagaaag ttaaaaatg 
      gttaacgaa gtgacagta gcaaaattc gtgcacagg 
      ggcgtggca aagtggtaa cgaaaatac gtttagaat 
      tagtttgat gatgataaa ctgctcgtt tggtaaaaa 
      atatacatt tactgcatt tatcaacaa aaagaaagt 
      tgaattatg aattattac ttaattaac tcagtcgtg 
      gacaggagc gtgctagtg gtaacgaaa atacgttta 
      gacgtgcta tgtggagac cccgcttta attgaatgt 
      ctgcttcca aatttatct ccaaaattg tctccaaaa 
      ttgtagggt ggtaacgaa aatatgttt agactcagt 
      tcggtaatg aagtggcca aaaaaaaat cgtaatcaa 
      caaaatgaa attgctatt tttattcac tttaaaaaa 
      ataaggcgt ggtaatgtg gtaacgaaa atatgttta 
      gaattagtt cggggatag tacgggcgt ggctgtcaa 
      ctggccacg cctttaaac caccatgac gtgaagata 
      tgccgactt tttgttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY