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Gene information
General information
TC004626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6302970..6306844
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004626
    iBeetle screen iB_08941

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004626 RA
      MGVSYDLFF LTFLFLLTC TFGYFGNVL DDDCFCQLH 
GKIDDCSCN VDTVDHFNN VKIYPRLRS LLHKGYFRF 
YKVNLRRPC PFWSDDSRC AMRYCHVEA CEDKDIPVG 
LKGGLENHS SKNLYVTQS CEDDHNAEL GYLNTTISA 
KVQEDIVLW SAYDDAQDN FCVLEDNDS ESEYVDLLL 
NPERYTGYR GKSAHRIWN SIYMENCFR STKNDNPYI 
QSSKLNNMC LEERVFYRA ISGLHASIN IHLCAQFLL 
SETSLSEPD GKWGPNLSE FLRRFSKET TRGEGPHWL 
RNLYFVYLL ELRALNKAM RYLEKEEFY TGNESEDWD 
TQLAVKDLL SVVQKFPNH FNETTMFTG SEQAEKLKY 
EFKQHFRNV SKIMDCVGC DKCRLWGKL QVQGLGTAL 
KILFSGKFD DQLDLSSDR KKFQLQRSE IVSLVNSIG 
RLSTSIYEL DEFRQMLR
    CDS sequence
    • >TC004626 RA
      atgggggtt agctacgat ttgtttttt ctcacgttt 
ttgttcctt ttgacttgc acgttcggg tactttggc 
aacgtcttg gacgatgat tgtttttgc cagcttcat 
ggaaaaatc gacgactgt tcgtgtaac gtagataca 
gtagaccac tttaataac gtaaaaatc taccccaga 
ttgcgcagt ttgctccac aaggggtat ttccgcttt 
tataaggtg aatttgcgg agaccatgt cccttttgg 
tctgacgac agtagatgt gccatgagg tactgtcac 
gtggaggct tgcgaagat aaagacatc cctgtgggg 
ctcaaaggc ggtttagaa aaccacagt tcaaaaaat 
ttgtatgtg actcaaagt tgtgaagac gatcacaac 
gccgagctg ggttacttg aatactaca attagcgca 
aaagtgcaa gaagacata gttctctgg agcgcttac 
gacgacgcc caagacaac ttttgcgtc cttgaggac 
aacgacagt gaatcggaa tacgtggat ttgcttcta 
aatccagaa cgatacact ggatacagg ggcaagtcg 
gcgcaccgt atttggaac agcatctac atggagaat 
tgcttcaga agcacgaaa aacgacaat ccgtacatc 
cagagctcc aagctcaac aatatgtgt ctggaggaa 
cgcgttttt taccgagct atttcgggc ctccacgct 
agcataaat atccacttg tgcgcgcaa tttttgctc 
tccgaaacg agtttgagc gaacctgac ggcaagtgg 
gggcccaat ttgagcgag tttttgaga cgcttctcg 
aaagagacc acgcgcggt gaaggcccc cactggctc 
cggaattta tattttgtt tacttgctc gagctgcga 
gcgttaaat aaagccatg cgttatttg gagaaagag 
gagttttac acggggaat gagagcgaa gactgggac 
acgcaactt gctgttaag gacctcctc agtgtcgtc 
caaaaattt ccaaaccac ttcaacgaa accaccatg 
ttcacgggc agcgaacag gcggagaag cttaagtac 
gaatttaag caacacttc cggaatgtg tccaaaatt 
atggactgt gtcgggtgc gataaatgc agattgtgg 
ggaaagtta caagtgcag ggcttagga actgcgttg 
aaaattctt ttttccggg aagtttgat gatcagctg 
gacttatca tcggacagg aagaaattc cagttgcaa 
agaagtgag attgtctca cttgtaaat agtatcgga 
aggctgtct acgagtatt tatgaattg gacgaattc 
cgtcaaatg ttacgatga
    mRNA sequence
    • >TC004626 RA
      attacctaa taaatgaat tttccgcaa ataatgccc 
agtttttta ggttacaat tttttgaca gcttttgtc 
aaactgtcg ataaaagcg cgcgcattc attggtgtg 
atatttcac ttgaaaatt atttttcca acaataata 
acaatataa ttcaaagaa aaaacattt tttacattt 
ataaaatta tcaaagacg acgttaaat cacacaatg 
cgtgaagat aacacgttt gctcgtagt tcggtcttg 
aggttagat gtcaactgc ctgccaaag tgtttgtca 
gtgttatca cttatcaga aaatgtggc agttttcaa 
aacaatttc aaatgagtg ttagtgtgt cactgtgga 
aacgtgata gtcgtaact taaaaaatc gtaattgat 
atgttgttg aaacgcctc cctgtaacc tagtgacga 
tgggggtta gctacgatt tgttttttc tcacgtttt 
tgttccttt tgacttgca cgttcgggt actttggca 
acgtcttgg acgatgatt gtttttgcc agcttcatg 
gaaaaatcg acgactgtt cgtgtaacg tagatacag 
tagaccact ttaataacg taaaaatct accccagat 
tgcgcagtt tgctccaca aggggtatt tccgctttt 
ataaggtga atttgcgga gaccatgtc ccttttggt 
ctgacgaca gtagatgtg ccatgaggt actgtcacg 
tggaggctt gcgaagata aagacatcc ctgtggggc 
tcaaaggcg gtttagaaa accacagtt caaaaaatt 
tgtatgtga ctcaaagtt gtgaagacg atcacaacg 
ccgagctgg gttacttga atactacaa ttagcgcaa 
aagtgcaag aagacatag ttctctgga gcgcttacg 
acgacgccc aagacaact tttgcgtcc ttgaggaca 
acgacagtg aatcggaat acgtggatt tgcttctaa 
atccagaac gatacactg gatacaggg gcaagtcgg 
cgcaccgta tttggaaca gcatctaca tggagaatt 
gcttcagaa gcacgaaaa acgacaatc cgtacatcc 
agagctcca agctcaaca atatgtgtc tggaggaac 
gcgtttttt accgagcta tttcgggcc tccacgcta 
gcataaata tccacttgt gcgcgcaat ttttgctct 
ccgaaacga gtttgagcg aacctgacg gcaagtggg 
ggcccaatt tgagcgagt ttttgagac gcttctcga 
aagagacca cgcgcggtg aaggccccc actggctcc 
ggaatttat attttgttt acttgctcg agctgcgag 
cgttaaata aagccatgc gttatttgg agaaagagg 
agttttaca cggggaatg agagcgaag actgggaca 
cgcaacttg ctgttaagg acctcctca gtgtcgtcc 
aaaaatttc caaaccact tcaacgaaa ccaccatgt 
tcacgggca gcgaacagg cggagaagc ttaagtacg 
aatttaagc aacacttcc ggaatgtgt ccaaaatta 
tggactgtg tcgggtgcg ataaatgca gattgtggg 
gaaagttac aagtgcagg gcttaggaa ctgcgttga 
aaattcttt tttccggga agtttgatg atcagctgg 
acttatcat cggacagga agaaattcc agttgcaaa 
gaagtgaga ttgtctcac ttgtaaata gtatcggaa 
ggctgtcta cgagtattt atgaattgg acgaattcc 
gtcaaatgt tacgatgac cttggtggt cgtcaagtg 
gcaaaaccg tttcataat ttaacaaag agcggtcgt 
gggaccaaa gtgtgcaga ggcgtgcta gaatggtaa 
cgaaaatat gtatagaat tagttcggt gatgtgtaa 
ctccgccct tacccaaaa tagtaacca aactacatt 
ttgaattaa tttggcagt gaactgcta gaaagttaa 
aaatggtta acgaagtga cggtagcaa aattcgtga 
acaggggcg tggcaaagt ggtaacgaa aataagttt 
agaattagt ttgatgatg ataaactgc tcgtttggg 
aaaaaatat acatttact gcatttatc aacaaaaag 
aaagttgaa ttatgaatt attacttaa ttaactcag 
tcgtggaca ggagcgtgc tagtggtaa cgaaaatac 
gtttagacg tggtatgtg gagaccccg ttttaattg 
aatgtctgc ttccaaaat tacctccaa aattgtagg 
gtggtaacg aaaatatgt ttagacttt tttcggtaa 
tgaaatggc caaaaaaaa tcgtaatcg acaaaatga 
aattgctat ttaggcgtg gtaatgtgg tcacgaaaa 
tacgtttag acgtgatat ctagcggcc acgctccta 
ttgctgcga caaaattat tttagaatc gtagtggta 
acgaaaata tgttcagac ttatttcga tgaagaaat 
gggcaaaaa tttaaaaaa gtagtcaac aaaatgaag 
ttacatttt tgattcacc taattaata aggcgtggt 
aacgaaaat acgtttaga attagttcg gggataata 
caggcgtgg ctgtgaact gaccacgcc tttaaacca 
ccatgacgt gaagatatg cgagactta tttacataa 
tttggcaaa aaaccgtaa aaatgtaag cagtagtgc 
gtaacagtg tggtcacga aaatacgtt tagacgtga 
tatctagcg gccacgctt tttttgctg cgacaaaat 
tattttaga atcgtagtg gtaacgaaa atatgttca 
gacttattt cgatgaagg aatgggcaa aaatttaaa 
aaagtagtc aacaaaatg aagttacaa tattgattc 
acctaatta ataaggcgt ggtaacgaa aatatgttc 
agacttatt tagatgaag aaatgggca aaaatttca 
aaaagtaaa atgtaatca gtagtgcat aacagtgtg 
gtaacgaaa aaacgttta gacgtgata tctagcggc 
cacgcccct gatgctgcg acaaaattg gtaacgaaa 
atatgttca gaattagtt cggtgatgt gtaactccg 
cccttaccc aaaacagta accaaacta tatttttaa 
ttaatttgg cagtgaact gctagaaag ttaaaaatg 
gttaacgaa gtgacagta gcaaaattc gtgcacagg 
ggcgtggca aagtggtaa cgaaaatac gtttagaat 
tagtttgat gatgataaa ctgctcgtt tggtaaaaa 
atatacatt tactgcatt tatcaacaa aaagaaagt 
tgaattatg aattattac ttaattaac tcagtcgtg 
gacaggagc gtgctagtg gtaacgaaa atacgttta 
gacgtgcta tgtggagac cccgcttta attgaatgt 
ctgcttcca aatttatct ccaaaattg tctccaaaa 
ttgtagggt ggtaacgaa aatatgttt agactcagt 
tcggtaatg aagtggcca aaaaaaaat cgtaatcaa 
caaaatgaa attgctatt tttattcac tttaaaaaa 
ataaggcgt ggtaatgtg gtaacgaaa atatgttta 
gaattagtt cggggatag tacgggcgt ggctgtcaa 
ctggccacg cctttaaac caccatgac gtgaagata 
tgccgactt tttgttg
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