| General information | |||
| TC004626 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6302970..6306844 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004626 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08941 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004626 RA
MGVSYDLFF LTFLFLLTC TFGYFGNVL DDDCFCQLH GKIDDCSCN VDTVDHFNN VKIYPRLRS LLHKGYFRF YKVNLRRPC PFWSDDSRC AMRYCHVEA CEDKDIPVG LKGGLENHS SKNLYVTQS CEDDHNAEL GYLNTTISA KVQEDIVLW SAYDDAQDN FCVLEDNDS ESEYVDLLL NPERYTGYR GKSAHRIWN SIYMENCFR STKNDNPYI QSSKLNNMC LEERVFYRA ISGLHASIN IHLCAQFLL SETSLSEPD GKWGPNLSE FLRRFSKET TRGEGPHWL RNLYFVYLL ELRALNKAM RYLEKEEFY TGNESEDWD TQLAVKDLL SVVQKFPNH FNETTMFTG SEQAEKLKY EFKQHFRNV SKIMDCVGC DKCRLWGKL QVQGLGTAL KILFSGKFD DQLDLSSDR KKFQLQRSE IVSLVNSIG RLSTSIYEL DEFRQMLR
- >TC004626 RA
atgggggtt agctacgat ttgtttttt ctcacgttt ttgttcctt ttgacttgc acgttcggg tactttggc aacgtcttg gacgatgat tgtttttgc cagcttcat ggaaaaatc gacgactgt tcgtgtaac gtagataca gtagaccac tttaataac gtaaaaatc taccccaga ttgcgcagt ttgctccac aaggggtat ttccgcttt tataaggtg aatttgcgg agaccatgt cccttttgg tctgacgac agtagatgt gccatgagg tactgtcac gtggaggct tgcgaagat aaagacatc cctgtgggg ctcaaaggc ggtttagaa aaccacagt tcaaaaaat ttgtatgtg actcaaagt tgtgaagac gatcacaac gccgagctg ggttacttg aatactaca attagcgca aaagtgcaa gaagacata gttctctgg agcgcttac gacgacgcc caagacaac ttttgcgtc cttgaggac aacgacagt gaatcggaa tacgtggat ttgcttcta aatccagaa cgatacact ggatacagg ggcaagtcg gcgcaccgt atttggaac agcatctac atggagaat tgcttcaga agcacgaaa aacgacaat ccgtacatc cagagctcc aagctcaac aatatgtgt ctggaggaa cgcgttttt taccgagct atttcgggc ctccacgct agcataaat atccacttg tgcgcgcaa tttttgctc tccgaaacg agtttgagc gaacctgac ggcaagtgg gggcccaat ttgagcgag tttttgaga cgcttctcg aaagagacc acgcgcggt gaaggcccc cactggctc cggaattta tattttgtt tacttgctc gagctgcga gcgttaaat aaagccatg cgttatttg gagaaagag gagttttac acggggaat gagagcgaa gactgggac acgcaactt gctgttaag gacctcctc agtgtcgtc caaaaattt ccaaaccac ttcaacgaa accaccatg ttcacgggc agcgaacag gcggagaag cttaagtac gaatttaag caacacttc cggaatgtg tccaaaatt atggactgt gtcgggtgc gataaatgc agattgtgg ggaaagtta caagtgcag ggcttagga actgcgttg aaaattctt ttttccggg aagtttgat gatcagctg gacttatca tcggacagg aagaaattc cagttgcaa agaagtgag attgtctca cttgtaaat agtatcgga aggctgtct acgagtatt tatgaattg gacgaattc cgtcaaatg ttacgatga
- >TC004626 RA
attacctaa taaatgaat tttccgcaa ataatgccc agtttttta ggttacaat tttttgaca gcttttgtc aaactgtcg ataaaagcg cgcgcattc attggtgtg atatttcac ttgaaaatt atttttcca acaataata acaatataa ttcaaagaa aaaacattt tttacattt ataaaatta tcaaagacg acgttaaat cacacaatg cgtgaagat aacacgttt gctcgtagt tcggtcttg aggttagat gtcaactgc ctgccaaag tgtttgtca gtgttatca cttatcaga aaatgtggc agttttcaa aacaatttc aaatgagtg ttagtgtgt cactgtgga aacgtgata gtcgtaact taaaaaatc gtaattgat atgttgttg aaacgcctc cctgtaacc tagtgacga tgggggtta gctacgatt tgttttttc tcacgtttt tgttccttt tgacttgca cgttcgggt actttggca acgtcttgg acgatgatt gtttttgcc agcttcatg gaaaaatcg acgactgtt cgtgtaacg tagatacag tagaccact ttaataacg taaaaatct accccagat tgcgcagtt tgctccaca aggggtatt tccgctttt ataaggtga atttgcgga gaccatgtc ccttttggt ctgacgaca gtagatgtg ccatgaggt actgtcacg tggaggctt gcgaagata aagacatcc ctgtggggc tcaaaggcg gtttagaaa accacagtt caaaaaatt tgtatgtga ctcaaagtt gtgaagacg atcacaacg ccgagctgg gttacttga atactacaa ttagcgcaa aagtgcaag aagacatag ttctctgga gcgcttacg acgacgccc aagacaact tttgcgtcc ttgaggaca acgacagtg aatcggaat acgtggatt tgcttctaa atccagaac gatacactg gatacaggg gcaagtcgg cgcaccgta tttggaaca gcatctaca tggagaatt gcttcagaa gcacgaaaa acgacaatc cgtacatcc agagctcca agctcaaca atatgtgtc tggaggaac gcgtttttt accgagcta tttcgggcc tccacgcta gcataaata tccacttgt gcgcgcaat ttttgctct ccgaaacga gtttgagcg aacctgacg gcaagtggg ggcccaatt tgagcgagt ttttgagac gcttctcga aagagacca cgcgcggtg aaggccccc actggctcc ggaatttat attttgttt acttgctcg agctgcgag cgttaaata aagccatgc gttatttgg agaaagagg agttttaca cggggaatg agagcgaag actgggaca cgcaacttg ctgttaagg acctcctca gtgtcgtcc aaaaatttc caaaccact tcaacgaaa ccaccatgt tcacgggca gcgaacagg cggagaagc ttaagtacg aatttaagc aacacttcc ggaatgtgt ccaaaatta tggactgtg tcgggtgcg ataaatgca gattgtggg gaaagttac aagtgcagg gcttaggaa ctgcgttga aaattcttt tttccggga agtttgatg atcagctgg acttatcat cggacagga agaaattcc agttgcaaa gaagtgaga ttgtctcac ttgtaaata gtatcggaa ggctgtcta cgagtattt atgaattgg acgaattcc gtcaaatgt tacgatgac cttggtggt cgtcaagtg gcaaaaccg tttcataat ttaacaaag agcggtcgt gggaccaaa gtgtgcaga ggcgtgcta gaatggtaa cgaaaatat gtatagaat tagttcggt gatgtgtaa ctccgccct tacccaaaa tagtaacca aactacatt ttgaattaa tttggcagt gaactgcta gaaagttaa aaatggtta acgaagtga cggtagcaa aattcgtga acaggggcg tggcaaagt ggtaacgaa aataagttt agaattagt ttgatgatg ataaactgc tcgtttggg aaaaaatat acatttact gcatttatc aacaaaaag aaagttgaa ttatgaatt attacttaa ttaactcag tcgtggaca ggagcgtgc tagtggtaa cgaaaatac gtttagacg tggtatgtg gagaccccg ttttaattg aatgtctgc ttccaaaat tacctccaa aattgtagg gtggtaacg aaaatatgt ttagacttt tttcggtaa tgaaatggc caaaaaaaa tcgtaatcg acaaaatga aattgctat ttaggcgtg gtaatgtgg tcacgaaaa tacgtttag acgtgatat ctagcggcc acgctccta ttgctgcga caaaattat tttagaatc gtagtggta acgaaaata tgttcagac ttatttcga tgaagaaat gggcaaaaa tttaaaaaa gtagtcaac aaaatgaag ttacatttt tgattcacc taattaata aggcgtggt aacgaaaat acgtttaga attagttcg gggataata caggcgtgg ctgtgaact gaccacgcc tttaaacca ccatgacgt gaagatatg cgagactta tttacataa tttggcaaa aaaccgtaa aaatgtaag cagtagtgc gtaacagtg tggtcacga aaatacgtt tagacgtga tatctagcg gccacgctt tttttgctg cgacaaaat tattttaga atcgtagtg gtaacgaaa atatgttca gacttattt cgatgaagg aatgggcaa aaatttaaa aaagtagtc aacaaaatg aagttacaa tattgattc acctaatta ataaggcgt ggtaacgaa aatatgttc agacttatt tagatgaag aaatgggca aaaatttca aaaagtaaa atgtaatca gtagtgcat aacagtgtg gtaacgaaa aaacgttta gacgtgata tctagcggc cacgcccct gatgctgcg acaaaattg gtaacgaaa atatgttca gaattagtt cggtgatgt gtaactccg cccttaccc aaaacagta accaaacta tatttttaa ttaatttgg cagtgaact gctagaaag ttaaaaatg gttaacgaa gtgacagta gcaaaattc gtgcacagg ggcgtggca aagtggtaa cgaaaatac gtttagaat tagtttgat gatgataaa ctgctcgtt tggtaaaaa atatacatt tactgcatt tatcaacaa aaagaaagt tgaattatg aattattac ttaattaac tcagtcgtg gacaggagc gtgctagtg gtaacgaaa atacgttta gacgtgcta tgtggagac cccgcttta attgaatgt ctgcttcca aatttatct ccaaaattg tctccaaaa ttgtagggt ggtaacgaa aatatgttt agactcagt tcggtaatg aagtggcca aaaaaaaat cgtaatcaa caaaatgaa attgctatt tttattcac tttaaaaaa ataaggcgt ggtaatgtg gtaacgaaa atatgttta gaattagtt cggggatag tacgggcgt ggctgtcaa ctggccacg cctttaaac caccatgac gtgaagata tgccgactt tttgttg
