Status
Please wait ...
Gene information
General information
TC034146
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006637
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12203951..12209553
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034146
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08940

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034146 RA
      MSVSAWLKS VAVRRVPIL GWAPQYSTD KLVSDAIAG 
      ITVGLTVMP QALAYATLA GLEPQYGLY SAFVGCFVY 
      TIFGTCKDI TIGPTALMA LMTYQQIIG RNTDYAILL 
      CFLCGVVQL VMAILHLGV LVDFISIPV TVGFTSATS 
      VIIMTSQIK SLLGLKISS SGFLDTITK VVKNIHHTR 
      MADLTLGMV CIAVLMLLR KLKDYKPSK KQRTLSKAL 
      WLISTSRNA LVVIVCSTV AYFYEIRGP GSPFRLTGT 
      VRPGLPDFR APPFGTTLH NRTVGFGEM VADLGTSVI 
      LVPIIAVLG NVAIAKAFA SGQMIDATQ ELMTLSMCN 
      VFGSFFSSM PITGSFSRS AVNHASGVR TPLGGVYTG 
      IMVLLALGF LTPYFAYIP KASLAAVII SAVIFMIEY 
      EVVKPMWRS SKKDLVATC ATFVFCLAI GVEYGILVG 
      VGINIIFLL YPSARPTVH VDKVKTRNN ALEYIMITP 
      GNSLYFPAV DFIKTSIGN AGISSKHLP VVIDCRFIL 
      GADFTAAKG ISALINEFL IRRQPLYFL HTRQEVVSL 
      FRGVFDNEF KYFTTREEL ESVLEDNKR KEIDSEETH 
      LLNNHNQWR HSMVELNEI SCEDSNICQ RKVPNKS
    CDS sequence
    • >TC034146 RA
      atgtccgtt agtgcgtgg ctgaagtca gtggcggtg 
      cgtcgggtg cccattctg gggtgggcg ccccagtac 
      agcactgac aagttggtg agcgacgcc atcgccggg 
      atcacagtt ggcctgact gtcatgcca caggccttg 
      gcgtatgcc actctggcc ggattggag ccacaatac 
      gggttgtat tcggcgttt gtcggttgc tttgtttac 
      acgattttt ggcacttgt aaggatata acgataggg 
      ccgacggcc cttatggcc ctcatgacg taccagcaa 
      attattggg cgtaatact gactacgcg attctgctt 
      tgttttttg tgtggggtt gtgcaactc gtcatggcc 
      atcttacat ttaggtgtt ctcgtcgat tttatctct 
      ataccggtg acagtcggt tttacgtca gctacgtct 
      gtgataatc atgacatct caaattaaa agtttgtta 
      ggtttgaaa atttcatcg tctgggttt ttagacaca 
      attaccaaa gttgtcaaa aatatacat cataccaga 
      atggcggat ttgacatta ggaatggtc tgcatcgcc 
      gtcttgatg cttctcagg aaactcaaa gattacaag 
      ccctctaaa aaacaacgc acattatct aaggccctt 
      tggttgata tcaacatca cgtaacgcc cttgtggtc 
      atcgtgtgc tcgacagtg gcgtacttt tatgaaatc 
      aggggtccg ggatctccg tttcgttta accggaacg 
      gtgcggccc ggactcccg gatttccgg gcgccacct 
      tttggcaca actttgcat aatcgcacc gttggcttc 
      ggcgaaatg gtcgccgat ttggggact tcggtgatt 
      ttggtgccc attattgcc gtcctgggc aacgttgcc 
      atagcaaag gccttcgcc agtggccaa atgatcgac 
      gcgactcaa gaactgatg accttgtcg atgtgtaac 
      gttttcgga tcgtttttt tcatcgatg ccgattacg 
      gggtcattc tcaaggtca gcggtgaat cacgccagt 
      ggcgtacgc acccctttg ggaggggtg tacaccggg 
      attatggtc ctactagcc ttgggtttc ctaacccct 
      tatttcgcc tacatacca aaggcgtct ttggcggct 
      gtgataatc agcgctgtc atttttatg atcgaatat 
      gaggtggtt aaacccatg tggaggagc agtaaaaag 
      gatctagtg gccacctgt gccacgttc gtcttttgt 
      ttagccata ggcgtcgaa tatgggatt ctagtgggc 
      gtggggatc aatattatt ttcctgttg tatccctcg 
      gcacggcct actgtgcac gtggacaaa gtcaaaacg 
      cgtaataac gcacttgag tacattatg ataactcca 
      gggaacagt ctttatttc ccggctgtt gattttatt 
      aagactagt atcggaaat gctgggatt agttccaaa 
      catttaccg gtggtcatt gactgtcgc tttatacta 
      ggggctgat tttaccgca gctaaggga atttcagcc 
      ttaattaac gaattttta atccgacga caaccgctc 
      tattttttg cacacaaga caggaagtt gtgtcgctc 
      tttcggggc gtttttgac aacgaattc aagtatttt 
      acgacacgt gaagaactg gaatcggtt ttagaagat 
      aacaaacgt aaagaaata gacagtgag gaaacccat 
      ttattaaat aaccacaac cagtggcgg cacagtatg 
      gtggaactg aacgaaatt tcctgcgaa gactcgaat 
      atttgccag cggaaagta ccgaataaa tcatga
    mRNA sequence
    • >TC034146 RA
      gtcgatttg tgtttaaca aaatggatt gatttttac 
      actcgtatc agtgtctaa ttttgtttt cgatcgtta 
      accacacga aatgtatta ctttctgta aagttagtc 
      ataaaaaaa agagatgat gttatgatt ttggccggt 
      tgctagggc aacgtgtca aaaacgcgt cacgttttt 
      gacactgaa tggggccat tgccctttg cccgcaaat 
      cgtccggtg aaagtcttt cacttttcc tccggtatt 
      tgaattgtt tggcgcgac cgttagtcg aaaaaccgt 
      tcattttgt ccgtaggtg cccccatgt ccgttagtg 
      cgtggctga agtcagtgg cggtgcgtc gggtgccca 
      ttctggggt gggcgcccc agtacagca ctgacaagt 
      tggtgagcg acgccatcg ccgggatca cagttggcc 
      tgactgtca tgccacagg ccttggcgt atgccactc 
      tggccggat tggagccac aatacgggt tgtattcgg 
      cgtttgtcg gttgctttg tttacacga tttttggca 
      cttgtaagg atataacga tagggccga cggccctta 
      tggccctca tgacgtacc agcaaatta ttgggcgta 
      atactgact acgcgattc tgctttgtt ttttgtgtg 
      gggttgtgc aactcgtca tggccatct tacatttag 
      gtgttctcg tcgatttta tctctatac cggtgacag 
      tcggtttta cgtcagcta cgtctgtga taatcatga 
      catctcaaa ttaaaagtt tgttaggtt tgaaaattt 
      catcgtctg ggtttttag acacaatta ccaaagttg 
      tcaaaaata tacatcata ccagaatgg cggatttga 
      cattaggaa tggtctgca tcgccgtct tgatgcttc 
      tcaggaaac tcaaagatt acaagccct ctaaaaaac 
      aacgcacat tatctaagg ccctttggt tgatatcaa 
      catcacgta acgcccttg tggtcatcg tgtgctcga 
      cagtggcgt acttttatg aaatcaggg gtccgggat 
      ctccgtttc gtttaaccg gaacggtgc ggcccggac 
      tcccggatt tccgggcgc caccttttg gcacaactt 
      tgcataatc gcaccgttg gcttcggcg aaatggtcg 
      ccgatttgg ggacttcgg tgattttgg tgcccatta 
      ttgccgtcc tgggcaacg ttgccatag caaaggcct 
      tcgccagtg gccaaatga tcgacgcga ctcaagaac 
      tgatgacct tgtcgatgt gtaacgttt tcggatcgt 
      ttttttcat cgatgccga ttacggggt cattctcaa 
      ggtcagcgg tgaatcacg ccagtggcg tacgcaccc 
      ctttgggag gggtgtaca ccgggatta tggtcctac 
      tagccttgg gtttcctaa ccccttatt tcgcctaca 
      taccaaagg cgtctttgg cggctgtga taatcagcg 
      ctgtcattt ttatgatcg aatatgagg tggttaaac 
      ccatgtgga ggagcagta aaaaggatc tagtggcca 
      cctgtgcca cgttcgtct tttgtttag ccataggcg 
      tcgaatatg ggattctag tgggcgtgg ggatcaata 
      ttattttcc tgttgtatc cctcggcac ggcctactg 
      tgcacgtgg acaaagtca aaacgcgta ataacgcac 
      ttgagtaca ttatgataa ctccaggga acagtcttt 
      atttcccgg ctgttgatt ttattaaga ctagtatcg 
      gaaatgctg ggattagtt ccaaacatt taccggtgg 
      tcattgact gtcgcttta tactagggg ctgatttta 
      ccgcagcta agggaattt cagccttaa ttaacgaat 
      ttttaatcc gacgacaac cgctctatt ttttgcaca 
      caagacagg aagttgtgt cgctctttc ggggcgttt 
      ttgacaacg aattcaagt attttacga cacgtgaag 
      aactggaat cggttttag aagataaca aacgtaaag 
      aaatagaca gtgaggaaa cccatttat taaataacc 
      acaaccagt ggcggcaca gtatggtgg aactgaacg 
      aaatttcct gcgaagact cgaatattt gccagcgga 
      aagtaccga ataaatcat gactccaag ttggcagta 
      ctgtcacgc cagtagtac caacttaat ttacaaacc 
      gtggtcact gcccagcat ccgtggcat ttctattta 
      tacgtgtaa ataaataag acagcaaga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY