Status
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Gene information
General information
TC034144
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006637
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12192471..12197099
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034144
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08939

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034144 RA
      MAVKRYKRL LHTRIPITR WLPLYTLPT LLQDLLAGI 
      TVGMTEIPQ GIAYAIVAG LPPEYGLYS GLIDGFVYA 
      VFGGCKDLN IGPTSILSL MLQPHVAKM GPDASILMT 
      FISGIMIFC LGVMHLGFV IQFFSYPII AGFICGGSF 
      QIASSQLKS LFGIPGKNG NFLESWKSV FENFSQVRK 
      WDTVLGVTS IVALVALKE IRVFGSLQS RPDWTPRRN 
      FLGKLIFLL SLGRNALIV IIGTLISYY LYEQKPFKI 
      TGNVSGGFP PFRPPPFST NFTGTESTF TDMVQGYGV 
      SLIFIPLLS ILEAVSIAK AFSKGRKLD ATQEMLALG 
      LCNTFGSFF GSMPVTGSF TRSAVNNAS GVRTPLAGI 
      FTSLLLLVA IAFLTPTFY YVPKATLAS VIIAAMFYL 
      FDFGAFALL WRTKKLDLV PFLATFLCS LLLGVDYGI 
      LIGASINLL FVLYASARP KFTITEEKV LNDGSKIFV 
      IMPRDTLFF PAAEFLRDT VLECDGSDV TVIINGKYI 
      RNMDTTVAK SISVLAHEL SLRKHKVMF VNFKESVMK 
      ICIGVDKKL ENFFFSEEY YNSVTKETS HELDSSRL
    CDS sequence
    • >TC034144 RA
      atggcagtc aaacgctac aaacgcctc ctccacacg 
      cgcatcccc atcacacga tggctccct ctctacacg 
      ctcccgacc ctcctccaa gacctcctg gccgggatc 
      acagtcggc atgacggaa atcccccaa ggcatcgcc 
      tacgcgatc gtagcggga cttccccca gaatacgga 
      ctctactcc ggcttgatc gacggcttc gtctacgcc 
      gttttcggg ggctgtaag gacctgaat attggcccc 
      acgtcaatc ctctccctc atgctgcag ccccacgtg 
      gccaaaatg gggcccgac gcctccatt ttgatgact 
      ttcatctcc ggaattatg attttctgt ctgggagtg 
      atgcatttg ggtttcgtt attcagttt ttctcgtat 
      ccgatcata gccggcttt atttgtggg gggtcgttt 
      cagattgcg tcctcgcag cttaaaagc ttgttcggg 
      attccgggg aagaacggg aattttctc gagagttgg 
      aaaagtgtt tttgagaat ttttcccaa gttaggaaa 
      tgggatacg gtgttggga gtcacgtct attgtggcc 
      ttagtggcg ttgaaggaa atccgtgtt tttgggagc 
      ctccaaagc cgccccgac tggaccccg cgccgcaat 
      ttcctcgga aaactcatt ttcctcctc tccctcggc 
      cgcaacgcc ctcatcgta atcatcggc accctaatt 
      tcctactac ttgtacgag caaaaaccc ttcaaaatc 
      accggcaac gtcagcggg ggattcccc ccattcaga 
      cccccgccc ttctccacc aacttcacc ggaaccgag 
      tccacgttc accgacatg gtccaaggc tacggcgtt 
      tctctcatt ttcatccct ttgctctca atcctcgaa 
      gctgtgtcc attgccaag gcgttcagt aaaggccgg 
      aaattggac gccacgcag gaaatgcta gctttggga 
      ctttgtaac acgtttggg tcgtttttc gggtcgatg 
      cccgtgacc ggatccttc acccggagc gccgtgaac 
      aacgcctcc ggggtccgg accccgcta gctgggatt 
      tttacctct ttgctgctg cttgtggcg attgctttt 
      ctcacgcct actttctac tacgtgccg aaggccacc 
      cttgccagt gttataata gcagcgatg ttttacttg 
      tttgatttt ggggccttt gccctgctg tggagaacc 
      aaaaaactt gatttggtc ccatttttg gccacgttt 
      ctctgcagt cttttgctt ggggtggat tatgggatt 
      ttaatcggg gcttcgatt aatttactc ttcgttttg 
      tatgcaagt gcacgtccg aaatttaca ataactgag 
      gagaaagtt ctaaatgat ggaagcaag atttttgtt 
      attatgccg agggacaca ctttttttt cctgcagct 
      gagtttcta agggacacg gttttagag tgtgacggg 
      agcgacgtt accgttatt attaacggg aaatatatc 
      aggaatatg gacacaact gtggctaag agtatttct 
      gtgcttgca catgagctc agcttgcgc aaacataaa 
      gtcatgttt gttaatttt aaagaatcg gtgatgaaa 
      atctgtatt ggggttgat aagaaacta gagaatttc 
      ttcttcagt gaagaatat tacaacagt gttactaaa 
      gagacgtct cacgagtta gattcatcc agactgtga
    mRNA sequence
    • >TC034144 RA
      taatcctcc acaataaaa tcctattag tttattaca 
      gtcacatgt gcaaaaaaa atcaaagat aagaaactc 
      ccccaaaac tggcagtgc tccacaatg gcagtcaaa 
      cgctacaaa cgcctcctc cacacgcgc atccccatc 
      acacgatgg ctccctctc tacacgctc ccgaccctc 
      ctccaagac ctcctggcc gggatcaca gtcggcatg 
      acggaaatc ccccaaggc atcgcctac gcgatcgta 
      gcgggactt cccccagaa tacggactc tactccggc 
      ttgatcgac ggcttcgtc tacgccgtt ttcgggggc 
      tgtaaggac ctgaatatt ggccccacg tcaatcctc 
      tccctcatg ctgcagccc cacgtggcc aaaatgggg 
      cccgacgcc tccattttg atgactttc atctccgga 
      attatgatt ttctgtctg ggagtgatg catttgggt 
      ttcgttatt cagtttttc tcgtatccg atcatagcc 
      ggctttatt tgtgggggg tcgtttcag attgcgtcc 
      tcgcagctt aaaagcttg ttcgggatt ccggggaag 
      aacgggaat tttctcgag agttggaaa agtgttttt 
      gagaatttt tcccaagtt aggaaatgg gatacggtg 
      ttgggagtc acgtctatt gtggcctta gtggcgttg 
      aaggaaatc cgtgttttt gggagcctc caaagccgc 
      cccgactgg accccgcgc cgcaatttc ctcggaaaa 
      ctcattttc ctcctctcc ctcggccgc aacgccctc 
      atcgtaatc atcggcacc ctaatttcc tactacttg 
      tacgagcaa aaacccttc aaaatcacc ggcaacgtc 
      agcggggga ttcccccca ttcagaccc ccgcccttc 
      tccaccaac ttcaccgga accgagtcc acgttcacc 
      gacatggtc caaggctac ggcgtttct ctcattttc 
      atccctttg ctctcaatc ctcgaagct gtgtccatt 
      gccaaggcg ttcagtaaa ggccggaaa ttggacgcc 
      acgcaggaa atgctagct ttgggactt tgtaacacg 
      tttgggtcg tttttcggg tcgatgccc gtgaccgga 
      tccttcacc cggagcgcc gtgaacaac gcctccggg 
      gtccggacc ccgctagct gggattttt acctctttg 
      ctgctgctt gtggcgatt gcttttctc acgcctact 
      ttctactac gtgccgaag gccaccctt gccagtgtt 
      ataatagca gcgatgttt tacttgttt gattttggg 
      gcctttgcc ctgctgtgg agaaccaaa aaacttgat 
      ttggtccca tttttggcc acgtttctc tgcagtctt 
      ttgcttggg gtggattat gggatttta atcggggct 
      tcgattaat ttactcttc gttttgtat gcaagtgca 
      cgtccgaaa tttacaata actgaggag aaagttcta 
      aatgatgga agcaagatt tttgttatt atgccgagg 
      gacacactt ttttttcct gcagctgag tttctaagg 
      gacacggtt ttagagtgt gacgggagc gacgttacc 
      gttattatt aacgggaaa tatatcagg aatatggac 
      acaactgtg gctaagagt atttctgtg cttgcacat 
      gagctcagc ttgcgcaaa cataaagtc atgtttgtt 
      aattttaaa gaatcggtg atgaaaatc tgtattggg 
      gttgataag aaactagag aatttcttc ttcagtgaa 
      gaatattac aacagtgtt actaaagag acgtctcac 
      gagttagat tcatccaga ctgtgatta agtgttgcc 
      aataaaaaa ataaaaaca ccaatgatc g
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