Status
Please wait ...
Gene information
General information
TC034133
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006607,TC006606 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11896938..11917209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034133
    iBeetle screen iB_08933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands content split - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    metathorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mesothorax structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034133 RA
      MGFFRSVIL GLQLQSLTS HFVYFLREN ARRLSRAAQ 
SSVGVYISA SSGVECFSR RPLRKRESA DGLKTAILS 
HLPPSTNSP ICYSCRKMW KKKQGKKYK INLIDKLSN 
AISVGYGMK QSLLDVGFQ HREGPLTQM SDHPRFQFR 
REPTERGQT GNVDVDISV TKEMMNRRI YECGAFPLA 
RWQTYDKMK RLIIFLFAT NAISCNNIH KDFQKSNDL 
FAASLYKEI VKDPKYAGK NVLISPLSA ETVLALAQS 
GCGDETSQE IRTVLHLPN DQNQIENLY KTVLPTLGV 
QSANKIYVK EKFTIRSEF TKIAKEVFG SDCENVNFS 
KEEAAGIMN QWVEQQTGK KIRDLISPG VLNNRTRLV 
LVNALHFKG EFSVPFPPG FTYKENFYA GNSQIQVET 
MHNPSGAKY LYFECHHLN AKFLELPFQ NEASITFIL 
PNEKNGLAA LEAQIGRVL VPHNVTRDL VEVSLPKFT 
IESGFELKN VLQNLGVSK AFNEAEADL SGIAGNKGD 
LVVDSVNQK TFVGVEEGG VEAAAAAFA SIPVPPSAV 
VEPSKHFKA DHPFLFYIK IKGVIAFLG RFLTPN
    CDS sequence
    • >TC034133 RA
      atggggttt tttcggagc gtgattttg gggttgcag 
cttcagtcc ttgacgtcc cattttgtg tatttcttg 
cgtgagaat gcgcgacgc ctaagtcgt gccgcacaa 
tcgtcagtt ggcgtctat atttcggcg tcgtcaggc 
gtcgagtgc ttctctcgt cgtccgtta cgaaaacga 
gaaagtgcc gatggatta aaaactgca attttgagt 
catcttccg ccttcaact aactcaccc atttgctat 
tcttgccgt aaaatgtgg aaaaaaaaa caggggaaa 
aaatacaaa atcaattta atcgacaag ctgagcaac 
gcaatcagt gttggttat ggcatgaag cagagcctc 
cttgacgta ggtttccag caccgggag ggcccgctg 
acgcagatg tccgatcac ccaagattt cagtttcga 
cgtgagcca actgagaga ggccaaacc ggaaatgtc 
gatgtcgat atctctgta acaaaagaa atgatgaat 
cggaggatt tacgaatgt ggtgctttt ccgctcgct 
cgctggcaa acttacgat aagatgaaa cgtttaata 
atctttttg ttcgcaact aatgcaatt tcttgtaat 
aatattcac aaagatttc caaaaatcg aatgattta 
ttcgctgct tccctctat aaagaaatt gtcaaggac 
cccaaatat gcgggcaaa aacgtgctc ataagcccg 
ctgtctgct gaaacagtg ttggctttg gcccagtct 
gggtgtggc gatgaaaca agccaagaa atccgaact 
gttctccat ctaccaaac gaccaaaac caaattgag 
aatttgtac aaaactgtg ctcccaaca ctgggcgtc 
caaagtgcc aataaaatt tacgtcaag gaaaaattc 
acaatccgg tccgaattt acgaaaatt gcaaaggaa 
gtgtttggc tccgattgc gaaaatgtg aatttttcc 
aaggaggaa gcggctggg attatgaac caatgggtt 
gagcagcaa accggcaaa aaaatccgc gatttgatc 
agtcctggt gttttgaat aacaggacc cgcttggtt 
ttggtcaat gctttgcac tttaagggg gaattttca 
gtcccgttt ccgccagga ttcacttat aaggaaaat 
ttctatgcg ggaaactcg caaatacaa gtggaaacc 
atgcacaac ccaagtggg gcaaaatat ttatatttt 
gagtgtcac catttgaac gccaaattc ctggaattg 
ccgtttcaa aatgaggct tcgatcact tttattttg 
cctaacgaa aaaaacggt ttggccgca cttgaggca 
caaatcggt cgggttttg gtgcctcat aatgtcact 
cgagacttg gtggaagtt tctttgccc aaattcaca 
atcgagagt ggtttcgag ttgaaaaac gttttgcaa 
aatttaggt gttagcaag gcttttaac gaggctgaa 
gccgatttg agcggaatt gctggaaat aagggcgat 
ttggtcgtt gatagtgtg aatcagaaa acgtttgtg 
ggggttgag gaggggggt gtggaagca gcagctgcc 
gcttttgcc agtattcca gttcctcca agtgccgta 
gtcgagcct tcgaagcac tttaaggcc gatcacccc 
ttcctgttc tatattaaa attaagggg gttattgcc 
ttcctcgga agattttta acaccgaat tga
    mRNA sequence
    • >TC034133 RA
      gcgccaact gttagaaac accatcaag ataaaggcg 
tagattttt ctgcccaaa gatggggtt ttttcggag 
cgtgatttt ggggttgca gcttcagtc cttgacgtc 
ccattttgt gtatttctt gcgtgagaa tgcgcgacg 
cctaagtcg tgccgcaca atcgtcagt tggcgtcta 
tatttcggc gtcgtcagg cgtcgagtg cttctctcg 
tcgtccgtt acgaaaacg agaaagtgc cgatggatt 
aaaaactgc aattttgag tcatcttcc gccttcaac 
taactcacc catttgcta ttcttgccg taaaatgtg 
gaaaaaaaa acaggggaa aaaatacaa aatcaattt 
aatcgacaa gctgagcaa cgcaatcag tgttggtta 
tggcatgaa gcagagcct ccttgacgt aggtttcca 
gcaccggga gggcccgct gacgcagat gtccgatca 
cccaagatt tcagtttcg acgtgagcc aactgagag 
aggccaaac cggaaatgt cgatgtcga tatctctgt 
aacaaaaga aatgatgaa tcggaggat ttacgaatg 
tggtgcttt tccgctcgc tcgctggca aacttacga 
taagatgaa acgtttaat aatcttttt gttcgcaac 
taatgcaat ttcttgtaa taatattca caaagattt 
ccaaaaatc gaatgattt attcgctgc ttccctcta 
taaagaaat tgtcaagga ccccaaata tgcgggcaa 
aaacgtgct cataagccc gctgtctgc tgaaacagt 
gttggcttt ggcccagtc tgggtgtgg cgatgaaac 
aagccaaga aatccgaac tgttctcca tctaccaaa 
cgaccaaaa ccaaattga gaatttgta caaaactgt 
gctcccaac actgggcgt ccaaagtgc caataaaat 
ttacgtcaa ggaaaaatt cacaatccg gtccgaatt 
tacgaaaat tgcaaagga agtgtttgg ctccgattg 
cgaaaatgt gaatttttc caaggagga agcggctgg 
gattatgaa ccaatgggt tgagcagca aaccggcaa 
aaaaatccg cgatttgat cagtcctgg tgttttgaa 
taacaggac ccgcttggt tttggtcaa tgctttgca 
ctttaaggg ggaattttc agtcccgtt tccgccagg 
attcactta taaggaaaa tttctatgc gggaaactc 
gcaaataca agtggaaac catgcacaa cccaagtgg 
ggcaaaata tttatattt tgagtgtca ccatttgaa 
cgccaaatt cctggaatt gccgtttca aaatgaggc 
ttcgatcac ttttatttt gcctaacga aaaaaacgg 
tttggccgc acttgaggc acaaatcgg tcgggtttt 
ggtgcctca taatgtcac tcgagactt ggtggaagt 
ttctttgcc caaattcac aatcgagag tggtttcga 
gttgaaaaa cgttttgca aaatttagg tgttagcaa 
ggcttttaa cgaggctga agccgattt gagcggaat 
tgctggaaa taagggcga tttggtcgt tgatagtgt 
gaatcagaa aacgtttgt gggggttga ggagggggg 
tgtggaagc agcagctgc cgcttttgc cagtattcc 
agttcctcc aagtgccgt agtcgagcc ttcgaagca 
ctttaaggc cgatcaccc cttcctgtt ctatattaa 
aattaaggg ggttattgc cttcctcgg aagattttt 
aacaccgaa ttgaatatc ttgtacaac aaaataaag 
ccgggtaac agat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY