General information | |||
TC034133
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 11896938..11917209 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC034133 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08933 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
metathorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
mesothorax structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034133 RA
MGFFRSVIL GLQLQSLTS HFVYFLREN ARRLSRAAQ SSVGVYISA SSGVECFSR RPLRKRESA DGLKTAILS HLPPSTNSP ICYSCRKMW KKKQGKKYK INLIDKLSN AISVGYGMK QSLLDVGFQ HREGPLTQM SDHPRFQFR REPTERGQT GNVDVDISV TKEMMNRRI YECGAFPLA RWQTYDKMK RLIIFLFAT NAISCNNIH KDFQKSNDL FAASLYKEI VKDPKYAGK NVLISPLSA ETVLALAQS GCGDETSQE IRTVLHLPN DQNQIENLY KTVLPTLGV QSANKIYVK EKFTIRSEF TKIAKEVFG SDCENVNFS KEEAAGIMN QWVEQQTGK KIRDLISPG VLNNRTRLV LVNALHFKG EFSVPFPPG FTYKENFYA GNSQIQVET MHNPSGAKY LYFECHHLN AKFLELPFQ NEASITFIL PNEKNGLAA LEAQIGRVL VPHNVTRDL VEVSLPKFT IESGFELKN VLQNLGVSK AFNEAEADL SGIAGNKGD LVVDSVNQK TFVGVEEGG VEAAAAAFA SIPVPPSAV VEPSKHFKA DHPFLFYIK IKGVIAFLG RFLTPN
- >TC034133 RA
atggggttt tttcggagc gtgattttg gggttgcag cttcagtcc ttgacgtcc cattttgtg tatttcttg cgtgagaat gcgcgacgc ctaagtcgt gccgcacaa tcgtcagtt ggcgtctat atttcggcg tcgtcaggc gtcgagtgc ttctctcgt cgtccgtta cgaaaacga gaaagtgcc gatggatta aaaactgca attttgagt catcttccg ccttcaact aactcaccc atttgctat tcttgccgt aaaatgtgg aaaaaaaaa caggggaaa aaatacaaa atcaattta atcgacaag ctgagcaac gcaatcagt gttggttat ggcatgaag cagagcctc cttgacgta ggtttccag caccgggag ggcccgctg acgcagatg tccgatcac ccaagattt cagtttcga cgtgagcca actgagaga ggccaaacc ggaaatgtc gatgtcgat atctctgta acaaaagaa atgatgaat cggaggatt tacgaatgt ggtgctttt ccgctcgct cgctggcaa acttacgat aagatgaaa cgtttaata atctttttg ttcgcaact aatgcaatt tcttgtaat aatattcac aaagatttc caaaaatcg aatgattta ttcgctgct tccctctat aaagaaatt gtcaaggac cccaaatat gcgggcaaa aacgtgctc ataagcccg ctgtctgct gaaacagtg ttggctttg gcccagtct gggtgtggc gatgaaaca agccaagaa atccgaact gttctccat ctaccaaac gaccaaaac caaattgag aatttgtac aaaactgtg ctcccaaca ctgggcgtc caaagtgcc aataaaatt tacgtcaag gaaaaattc acaatccgg tccgaattt acgaaaatt gcaaaggaa gtgtttggc tccgattgc gaaaatgtg aatttttcc aaggaggaa gcggctggg attatgaac caatgggtt gagcagcaa accggcaaa aaaatccgc gatttgatc agtcctggt gttttgaat aacaggacc cgcttggtt ttggtcaat gctttgcac tttaagggg gaattttca gtcccgttt ccgccagga ttcacttat aaggaaaat ttctatgcg ggaaactcg caaatacaa gtggaaacc atgcacaac ccaagtggg gcaaaatat ttatatttt gagtgtcac catttgaac gccaaattc ctggaattg ccgtttcaa aatgaggct tcgatcact tttattttg cctaacgaa aaaaacggt ttggccgca cttgaggca caaatcggt cgggttttg gtgcctcat aatgtcact cgagacttg gtggaagtt tctttgccc aaattcaca atcgagagt ggtttcgag ttgaaaaac gttttgcaa aatttaggt gttagcaag gcttttaac gaggctgaa gccgatttg agcggaatt gctggaaat aagggcgat ttggtcgtt gatagtgtg aatcagaaa acgtttgtg ggggttgag gaggggggt gtggaagca gcagctgcc gcttttgcc agtattcca gttcctcca agtgccgta gtcgagcct tcgaagcac tttaaggcc gatcacccc ttcctgttc tatattaaa attaagggg gttattgcc ttcctcgga agattttta acaccgaat tga
- >TC034133 RA
gcgccaact gttagaaac accatcaag ataaaggcg tagattttt ctgcccaaa gatggggtt ttttcggag cgtgatttt ggggttgca gcttcagtc cttgacgtc ccattttgt gtatttctt gcgtgagaa tgcgcgacg cctaagtcg tgccgcaca atcgtcagt tggcgtcta tatttcggc gtcgtcagg cgtcgagtg cttctctcg tcgtccgtt acgaaaacg agaaagtgc cgatggatt aaaaactgc aattttgag tcatcttcc gccttcaac taactcacc catttgcta ttcttgccg taaaatgtg gaaaaaaaa acaggggaa aaaatacaa aatcaattt aatcgacaa gctgagcaa cgcaatcag tgttggtta tggcatgaa gcagagcct ccttgacgt aggtttcca gcaccggga gggcccgct gacgcagat gtccgatca cccaagatt tcagtttcg acgtgagcc aactgagag aggccaaac cggaaatgt cgatgtcga tatctctgt aacaaaaga aatgatgaa tcggaggat ttacgaatg tggtgcttt tccgctcgc tcgctggca aacttacga taagatgaa acgtttaat aatcttttt gttcgcaac taatgcaat ttcttgtaa taatattca caaagattt ccaaaaatc gaatgattt attcgctgc ttccctcta taaagaaat tgtcaagga ccccaaata tgcgggcaa aaacgtgct cataagccc gctgtctgc tgaaacagt gttggcttt ggcccagtc tgggtgtgg cgatgaaac aagccaaga aatccgaac tgttctcca tctaccaaa cgaccaaaa ccaaattga gaatttgta caaaactgt gctcccaac actgggcgt ccaaagtgc caataaaat ttacgtcaa ggaaaaatt cacaatccg gtccgaatt tacgaaaat tgcaaagga agtgtttgg ctccgattg cgaaaatgt gaatttttc caaggagga agcggctgg gattatgaa ccaatgggt tgagcagca aaccggcaa aaaaatccg cgatttgat cagtcctgg tgttttgaa taacaggac ccgcttggt tttggtcaa tgctttgca ctttaaggg ggaattttc agtcccgtt tccgccagg attcactta taaggaaaa tttctatgc gggaaactc gcaaataca agtggaaac catgcacaa cccaagtgg ggcaaaata tttatattt tgagtgtca ccatttgaa cgccaaatt cctggaatt gccgtttca aaatgaggc ttcgatcac ttttatttt gcctaacga aaaaaacgg tttggccgc acttgaggc acaaatcgg tcgggtttt ggtgcctca taatgtcac tcgagactt ggtggaagt ttctttgcc caaattcac aatcgagag tggtttcga gttgaaaaa cgttttgca aaatttagg tgttagcaa ggcttttaa cgaggctga agccgattt gagcggaat tgctggaaa taagggcga tttggtcgt tgatagtgt gaatcagaa aacgtttgt gggggttga ggagggggg tgtggaagc agcagctgc cgcttttgc cagtattcc agttcctcc aagtgccgt agtcgagcc ttcgaagca ctttaaggc cgatcaccc cttcctgtt ctatattaa aattaaggg ggttattgc cttcctcgg aagattttt aacaccgaa ttgaatatc ttgtacaac aaaataaag ccgggtaac agat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
