Status
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Gene information
General information
TC034133
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006607,TC006606
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11896938..11917209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034133
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content split - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    metathorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mesothorax structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034133 RA
      MGFFRSVIL GLQLQSLTS HFVYFLREN ARRLSRAAQ 
      SSVGVYISA SSGVECFSR RPLRKRESA DGLKTAILS 
      HLPPSTNSP ICYSCRKMW KKKQGKKYK INLIDKLSN 
      AISVGYGMK QSLLDVGFQ HREGPLTQM SDHPRFQFR 
      REPTERGQT GNVDVDISV TKEMMNRRI YECGAFPLA 
      RWQTYDKMK RLIIFLFAT NAISCNNIH KDFQKSNDL 
      FAASLYKEI VKDPKYAGK NVLISPLSA ETVLALAQS 
      GCGDETSQE IRTVLHLPN DQNQIENLY KTVLPTLGV 
      QSANKIYVK EKFTIRSEF TKIAKEVFG SDCENVNFS 
      KEEAAGIMN QWVEQQTGK KIRDLISPG VLNNRTRLV 
      LVNALHFKG EFSVPFPPG FTYKENFYA GNSQIQVET 
      MHNPSGAKY LYFECHHLN AKFLELPFQ NEASITFIL 
      PNEKNGLAA LEAQIGRVL VPHNVTRDL VEVSLPKFT 
      IESGFELKN VLQNLGVSK AFNEAEADL SGIAGNKGD 
      LVVDSVNQK TFVGVEEGG VEAAAAAFA SIPVPPSAV 
      VEPSKHFKA DHPFLFYIK IKGVIAFLG RFLTPN
    CDS sequence
    • >TC034133 RA
      atggggttt tttcggagc gtgattttg gggttgcag 
      cttcagtcc ttgacgtcc cattttgtg tatttcttg 
      cgtgagaat gcgcgacgc ctaagtcgt gccgcacaa 
      tcgtcagtt ggcgtctat atttcggcg tcgtcaggc 
      gtcgagtgc ttctctcgt cgtccgtta cgaaaacga 
      gaaagtgcc gatggatta aaaactgca attttgagt 
      catcttccg ccttcaact aactcaccc atttgctat 
      tcttgccgt aaaatgtgg aaaaaaaaa caggggaaa 
      aaatacaaa atcaattta atcgacaag ctgagcaac 
      gcaatcagt gttggttat ggcatgaag cagagcctc 
      cttgacgta ggtttccag caccgggag ggcccgctg 
      acgcagatg tccgatcac ccaagattt cagtttcga 
      cgtgagcca actgagaga ggccaaacc ggaaatgtc 
      gatgtcgat atctctgta acaaaagaa atgatgaat 
      cggaggatt tacgaatgt ggtgctttt ccgctcgct 
      cgctggcaa acttacgat aagatgaaa cgtttaata 
      atctttttg ttcgcaact aatgcaatt tcttgtaat 
      aatattcac aaagatttc caaaaatcg aatgattta 
      ttcgctgct tccctctat aaagaaatt gtcaaggac 
      cccaaatat gcgggcaaa aacgtgctc ataagcccg 
      ctgtctgct gaaacagtg ttggctttg gcccagtct 
      gggtgtggc gatgaaaca agccaagaa atccgaact 
      gttctccat ctaccaaac gaccaaaac caaattgag 
      aatttgtac aaaactgtg ctcccaaca ctgggcgtc 
      caaagtgcc aataaaatt tacgtcaag gaaaaattc 
      acaatccgg tccgaattt acgaaaatt gcaaaggaa 
      gtgtttggc tccgattgc gaaaatgtg aatttttcc 
      aaggaggaa gcggctggg attatgaac caatgggtt 
      gagcagcaa accggcaaa aaaatccgc gatttgatc 
      agtcctggt gttttgaat aacaggacc cgcttggtt 
      ttggtcaat gctttgcac tttaagggg gaattttca 
      gtcccgttt ccgccagga ttcacttat aaggaaaat 
      ttctatgcg ggaaactcg caaatacaa gtggaaacc 
      atgcacaac ccaagtggg gcaaaatat ttatatttt 
      gagtgtcac catttgaac gccaaattc ctggaattg 
      ccgtttcaa aatgaggct tcgatcact tttattttg 
      cctaacgaa aaaaacggt ttggccgca cttgaggca 
      caaatcggt cgggttttg gtgcctcat aatgtcact 
      cgagacttg gtggaagtt tctttgccc aaattcaca 
      atcgagagt ggtttcgag ttgaaaaac gttttgcaa 
      aatttaggt gttagcaag gcttttaac gaggctgaa 
      gccgatttg agcggaatt gctggaaat aagggcgat 
      ttggtcgtt gatagtgtg aatcagaaa acgtttgtg 
      ggggttgag gaggggggt gtggaagca gcagctgcc 
      gcttttgcc agtattcca gttcctcca agtgccgta 
      gtcgagcct tcgaagcac tttaaggcc gatcacccc 
      ttcctgttc tatattaaa attaagggg gttattgcc 
      ttcctcgga agattttta acaccgaat tga
    mRNA sequence
    • >TC034133 RA
      gcgccaact gttagaaac accatcaag ataaaggcg 
      tagattttt ctgcccaaa gatggggtt ttttcggag 
      cgtgatttt ggggttgca gcttcagtc cttgacgtc 
      ccattttgt gtatttctt gcgtgagaa tgcgcgacg 
      cctaagtcg tgccgcaca atcgtcagt tggcgtcta 
      tatttcggc gtcgtcagg cgtcgagtg cttctctcg 
      tcgtccgtt acgaaaacg agaaagtgc cgatggatt 
      aaaaactgc aattttgag tcatcttcc gccttcaac 
      taactcacc catttgcta ttcttgccg taaaatgtg 
      gaaaaaaaa acaggggaa aaaatacaa aatcaattt 
      aatcgacaa gctgagcaa cgcaatcag tgttggtta 
      tggcatgaa gcagagcct ccttgacgt aggtttcca 
      gcaccggga gggcccgct gacgcagat gtccgatca 
      cccaagatt tcagtttcg acgtgagcc aactgagag 
      aggccaaac cggaaatgt cgatgtcga tatctctgt 
      aacaaaaga aatgatgaa tcggaggat ttacgaatg 
      tggtgcttt tccgctcgc tcgctggca aacttacga 
      taagatgaa acgtttaat aatcttttt gttcgcaac 
      taatgcaat ttcttgtaa taatattca caaagattt 
      ccaaaaatc gaatgattt attcgctgc ttccctcta 
      taaagaaat tgtcaagga ccccaaata tgcgggcaa 
      aaacgtgct cataagccc gctgtctgc tgaaacagt 
      gttggcttt ggcccagtc tgggtgtgg cgatgaaac 
      aagccaaga aatccgaac tgttctcca tctaccaaa 
      cgaccaaaa ccaaattga gaatttgta caaaactgt 
      gctcccaac actgggcgt ccaaagtgc caataaaat 
      ttacgtcaa ggaaaaatt cacaatccg gtccgaatt 
      tacgaaaat tgcaaagga agtgtttgg ctccgattg 
      cgaaaatgt gaatttttc caaggagga agcggctgg 
      gattatgaa ccaatgggt tgagcagca aaccggcaa 
      aaaaatccg cgatttgat cagtcctgg tgttttgaa 
      taacaggac ccgcttggt tttggtcaa tgctttgca 
      ctttaaggg ggaattttc agtcccgtt tccgccagg 
      attcactta taaggaaaa tttctatgc gggaaactc 
      gcaaataca agtggaaac catgcacaa cccaagtgg 
      ggcaaaata tttatattt tgagtgtca ccatttgaa 
      cgccaaatt cctggaatt gccgtttca aaatgaggc 
      ttcgatcac ttttatttt gcctaacga aaaaaacgg 
      tttggccgc acttgaggc acaaatcgg tcgggtttt 
      ggtgcctca taatgtcac tcgagactt ggtggaagt 
      ttctttgcc caaattcac aatcgagag tggtttcga 
      gttgaaaaa cgttttgca aaatttagg tgttagcaa 
      ggcttttaa cgaggctga agccgattt gagcggaat 
      tgctggaaa taagggcga tttggtcgt tgatagtgt 
      gaatcagaa aacgtttgt gggggttga ggagggggg 
      tgtggaagc agcagctgc cgcttttgc cagtattcc 
      agttcctcc aagtgccgt agtcgagcc ttcgaagca 
      ctttaaggc cgatcaccc cttcctgtt ctatattaa 
      aattaaggg ggttattgc cttcctcgg aagattttt 
      aacaccgaa ttgaatatc ttgtacaac aaaataaag 
      ccgggtaac agat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY