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Gene information
General information
TC006605 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11863505..11865138
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006605
    iBeetle screen iB_08932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006605 RA
      MAAIKLFSN NLKIIKGSV PTLSLIIQK 
      NGMSQYPSY YIQPVYKKE VQEELAKTG 
      EAQKLTHMP TRAALNNQT CSHTQDDLV 
      NLFTNYVMK DGLKPLARG LIEQAFENI 
      KRIQLEKYH KCQDPEIKA QIELDPRAI 
      FHKAVENCK PIMNLTPIK RGGVRYQVP 
      VPITPKRSQ FMSMKWLIA ASKEKDKNV 
      RFHYQLALE LIDAANNKG RVVKRKQDL 
      HKQCEANRA YAHYRWS
    CDS sequence
    • >TC006605 RA
      atggctgcg attaagtta ttttcaaat aatttgaaa 
      attattaaa ggaagtgtc cccacttta agcctaatt 
      atccagaaa aatggcatg tcgcagtac ccaagttat 
      tacatacag ccggtctat aagaaggag gttcaagaa 
      gagttggct aaaaccggc gaggcccag aaattgacc 
      cacatgccc acgagggca gccctcaat aaccagact 
      tgttcgcac actcaggat gacttagtc aatcttttc 
      accaattat gtcatgaag gacgggctc aaacctctg 
      gctaggggg ttaatagaa caggcgttt gaaaacatt 
      aaaagaatc caattggaa aagtaccac aagtgccag 
      gaccccgaa atcaaagcc cagattgag ttggaccct 
      cgtgcgatt ttccacaaa gctgtggag aattgtaag 
      ccgataatg aacctaaca cccataaaa cgtgggggt 
      gttaggtat caagtccca gtgccaatc acgccaaag 
      cggagccag ttcatgtcg atgaagtgg ctcatagcg 
      gcgagcaaa gagaaagat aagaatgtc aggttccat 
      tatcagcta gcgctggag ctaattgac gccgcaaat 
      aataaagga agagtagtt aagcggaaa caagacttg 
      cataagcag tgcgaggcc aatagggct tacgcacat 
      tatagatgg agctaa
    mRNA sequence
    • >TC006605 RA
      tgtgtgcgg cgggcatgc gcacgtgtt aacatggct 
      gcgattaag ttattttca aataatttg aaaattatt 
      aaaggaagt gtccccact ttaagccta attatccag 
      aaaaatggc atgtcgcag tacccaagt tattacata 
      cagccggtc tataagaag gaggttcaa gaagagttg 
      gctaaaacc ggcgaggcc cagaaattg acccacatg 
      cccacgagg gcagccctc aataaccag acttgttcg 
      cacactcag gatgactta gtcaatctt ttcaccaat 
      tatgtcatg aaggacggg ctcaaacct ctggctagg 
      gggttaata gaacaggcg tttgaaaac attaaaaga 
      atccaattg gaaaagtac cacaagtgc caggacccc 
      gaaatcaaa gcccagatt gagttggac cctcgtgcg 
      attttccac aaagctgtg gagaattgt aagccgata 
      atgaaccta acacccata aaacgtggg ggtgttagg 
      tatcaagtc ccagtgcca atcacgcca aagcggagc 
      cagttcatg tcgatgaag tggctcata gcggcgagc 
      aaagagaaa gataagaat gtcaggttc cattatcag 
      ctagcgctg gagctaatt gacgccgca aataataaa 
      ggaagagta gttaagcgg aaacaagac ttgcataag 
      cagtgcgag gccaatagg gcttacgca cattataga 
      tggagctaa acttaataa acacctcgt aaattatga 
      atattttat tgaagcctg cttgatcta agacaccat 
      accttacac acaacttaa gtgtttctt tggatgaaa 
      actgctgaa tttttaaat acagagggt cggttaaag 
      accgcgaca gctccgaaa gtggcacta tgtctttca 
      aaggcacca caacgtctt tccgggacc cccaaaagt 
      ccaaatagg caaacttga cttgctctt gtttttcat 
      ttaaataca ctattccga ctagtttat ttgcaaagt 
      aaccgagac cgtacaaag gcacaattc ccgcaattc 
      caagagttg cagtgaaca ggacattgt cgtgcgaaa 
      gaatgttta actgattta aaattgcag ttgttggta 
      gcgcgactg aggttagaa gagtttggt ataatttga 
      gacggttga ttaggccta cgccccgga cgaagggga 
      acttataaa ctgtcttaa attcgctct gtgttacgg 
      tacacaaag gcttaagtt ggttgtact gaggggaaa 
      taagccgaa tgagagaca tcctaaact aaaaaaaga 
      gacttattt ttgtataat ttaacaaga gggactcac 
      tgctgcttt attacatta tataattac tgcaagtgg 
      taagaaaag tgcatattt gtgcttata atgacacaa 
      atcagcaag tcctactgt tgaggttag atttagcct 
      atgcaacct gtaatacag tgtagggga cgtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY