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Gene information
General information
TC006463 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9985691..9987051
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006463
    iBeetle screen iB_08922

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006463 RA
      MKVPVLIVL ALVGLSSAK YEGYKVYKF VTKTHLQNV 
      YLQSLEAIP DFDFWSKIN TVGNPVTVM VPPRFQTKI 
      EDYLKLNNL EFSIEIENV GSAIHAEKH YHKSRQALG 
      LGKITFDQY LRHAEINAY LAQLAKDYP DTVILETIG 
      QSYEKRDMN LVRISSGPR DPPKPVIFV DAGIHAREW 
      IAPAVALYL INQLVENPS NSNLLEAVD WIVLPSVNP 
      DGYEFTWTG DRLWRKTRS PGTVCFGCD PNRNFGFHW 
      MEAGASSWE CSDTYAGKE AFSEVEARN LRDYLAKTA 
      NIKAYLTLH SYGQYLLYP WGYGDVLCD NWKELDDLG 
      HKVDDAISS VNGTRYTIG SSTQVLYAA AGASDDWAM 
      GGAGIDIVY TIELPGGGN YGFDLPASR IKGVVAETF 
      EGFKVFADY VAKNRK
    CDS sequence
    • >TC006463 RA
      atgaaagtg ccagtactg atagtacta gccctcgtg 
      ggcttgtct agtgccaaa tacgagggc tacaaagtt 
      tacaaattc gtaacaaaa acgcacctc cagaatgtg 
      tatctccag tctttggaa gcaatccca gacttcgat 
      ttctggtcg aaaattaac acagtgggt aacccagtc 
      accgtcatg gtaccaccg cgatttcaa acaaaaatt 
      gaggattat ttaaaactc aacaatctt gaatttagc 
      attgaaatc gaaaatgtt ggaagcgcc atccacgct 
      gaaaaacac taccacaaa tcgagacaa gctttaggt 
      ttgggaaaa attactttt gaccaatat ttacgccac 
      gctgagatt aatgcttat ttggcccaa ctggctaaa 
      gactacccc gacactgtc attctagaa acaatcggg 
      cagtcgtac gaaaaacgc gacatgaac ctagtccgg 
      atttcctcc gggcctaga gacccccca aagccggtg 
      attttcgtc gatgccggc atccacgcc cgcgagtgg 
      atcgcccca gccgtggcc ctctacctg ataaaccaa 
      ctggtggag aacccctcc aacagtaac cttctcgaa 
      gccgtggac tggatcgtc ctcccgagc gtcaacccc 
      gacggttac gagttcacc tggaccgga gaccgcctc 
      tggcgcaag acccgctcg ccagggacc gtctgcttc 
      ggctgcgac cctaatcgc aactttggg ttccactgg 
      atggaggct ggggccagt agctgggaa tgctcggac 
      acctacgcc ggaaaggag gcgttctcg gaggtggag 
      gcgaggaat ttgagagac tacttggcc aagactgcg 
      aatatcaag gcctatttg acactgcat agctatggg 
      caatatttg ctctacccg tggggctat ggggatgtt 
      ctctgtgac aattggaag gaattggac gacttgggg 
      cataaagta gacgatgct ataagttcg gttaatggg 
      acaagatac actattggt agttcgacc caagttttg 
      tatgccgca gctggggct agtgacgac tgggccatg 
      ggtggtgct gggatcgat attgtctat accattgag 
      ttgccaggg ggtgggaac tatgggttt gatttgccg 
      gcctcaagg attaagggg gtggtagcc gaaacgttc 
      gaaggattt aaagtgttt gcggactat gtcgcaaaa 
      aatagaaaa tag
    mRNA sequence
    • >TC006463 RA
      cgatacctc agtagatat cgcaatgaa agtgccagt 
      actgatagt actagccct cgtgggctt gtctagtgc 
      caaatacga gggctacaa agtttacaa attcgtaac 
      aaaaacgca cctccagaa tgtgtatct ccagtcttt 
      ggaagcaat cccagactt cgatttctg gtcgaaaat 
      taacacagt gggtaaccc agtcaccgt catggtacc 
      accgcgatt tcaaacaaa aattgagga ttatttaaa 
      actcaacaa tcttgaatt tagcattga aatcgaaaa 
      tgttggaag cgccatcca cgctgaaaa acactacca 
      caaatcgag acaagcttt aggtttggg aaaaattac 
      ttttgacca atatttacg ccacgctga gattaatgc 
      ttatttggc ccaactggc taaagacta ccccgacac 
      tgtcattct agaaacaat cgggcagtc gtacgaaaa 
      acgcgacat gaacctagt ccggatttc ctccgggcc 
      tagagaccc cccaaagcc ggtgatttt cgtcgatgc 
      cggcatcca cgcccgcga gtggatcgc cccagccgt 
      ggccctcta cctgataaa ccaactggt ggagaaccc 
      ctccaacag taaccttct cgaagccgt ggactggat 
      cgtcctccc gagcgtcaa ccccgacgg ttacgagtt 
      cacctggac cggagaccg cctctggcg caagacccg 
      ctcgccagg gaccgtctg cttcggctg cgaccctaa 
      tcgcaactt tgggttcca ctggatgga ggctggggc 
      cagtagctg ggaatgctc ggacaccta cgccggaaa 
      ggaggcgtt ctcggaggt ggaggcgag gaatttgag 
      agactactt ggccaagac tgcgaatat caaggccta 
      tttgacact gcatagcta tgggcaata tttgctcta 
      cccgtgggg ctatgggga tgttctctg tgacaattg 
      gaaggaatt ggacgactt ggggcataa agtagacga 
      tgctataag ttcggttaa tgggacaag atacactat 
      tggtagttc gacccaagt tttgtatgc cgcagctgg 
      ggctagtga cgactgggc catgggtgg tgctgggat 
      cgatattgt ctataccat tgagttgcc agggggtgg 
      gaactatgg gtttgattt gccggcctc aaggattaa 
      gggggtggt agccgaaac gttcgaagg atttaaagt 
      gtttgcgga ctatgtcgc aaaaaatag aaaatagat 
      aaaccattt gaaagtgaa cttttaaga ggaataaaa 
      taaactatt tggtacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY