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Gene information
General information
TC005668 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9930967..9933639
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005668
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08921

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber stage sequence irregular - Number of animals showing the phenotype: 4
    vitellarium size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005668 RA
      MMSPTEVSK KIKDYETFL EDTLKRDLA 
      DVQNILKDK VKKYKEWEE VQQVTKTIN 
      EFKEKDRDM VVRVGLGCG VHVSGEVSD 
      YERTFVNIG LGCLLEMDC DEADKYSNI 
      RMRSLKKDI DHYRSLAVH VKVNIKMVL 
      LAISELQSL MLNK
    CDS sequence
    • >TC005668 RA
      atgatgagc cccacggaa gtaagcaaa aaaataaag 
      gattacgag acttttctt gaagatact ttgaagagg 
      gacttggcc gacgtgcaa aacatcctc aaggataaa 
      gtgaaaaaa tacaaagaa tgggaggaa gtgcagcag 
      gtgacaaaa acaataaac gaattcaag gagaaagac 
      cgtgacatg gtcgtccgg gtaggtttg ggttgtggt 
      gttcacgta agtggtgaa gtttccgat tatgaacgg 
      acttttgta aatatcggg ctcggttgt ttgttggag 
      atggactgt gatgaagct gacaagtat tccaacatc 
      agaatgagg agtttgaag aaggacatt gaccattac 
      agaagcttg gcagtgcat gtgaaggtg aacatcaag 
      atggtcctg ttggctata agtgagctg caaagcctg 
      atgttgaat aaataa
    mRNA sequence
    • >TC005668 RA
      tttacaatt aaaatttat ctattttag ctgtttttt 
      tttaaactg attttttta atgacattt gaatttgac 
      aggtggttt gagcggttt gaggttagt agaagagaa 
      acggagctg gcgattgtg tgcaaaaaa cagcccaaa 
      agcgtgtac aactagcga tttgtactt acataaccc 
      caaactctc ctgtcaaag ccatgcatg caagatgat 
      gagccccac ggaagtaag caaaaaaat aaaggatta 
      cgagacttt tcttgaaga tactttgaa gagggactt 
      ggccgacgt gcaaaacat cctcaagga taaagtgaa 
      aaaatacaa agaatggga ggaagtgca gcaggtgac 
      aaaaacaat aaacgaatt caaggagaa agaccgtga 
      catggtcgt ccgggtagg tttgggttg tggtgttca 
      cgtaagtgg tgaagtttc cgattatga acggacttt 
      tgtaaatat cgggctcgg ttgtttgtt ggagatgga 
      ctgtgatga agctgacaa gtattccaa catcagaat 
      gaggagttt gaagaagga cattgacca ttacagaag 
      cttggcagt gcatgtgaa ggtgaacat caagatggt 
      cctgttggc tataagtga gctgcaaag cctgatgtt 
      gaataaata aacccttaa ctttcaaaa caattttat 
      tttaaaata tttaaattt aagctatta cacaacatg 
      tgaacacct tttaataag tcaatactt acatttaca 
      ttacaaaac tatggtata aaactagcg gactacgta 
      caaaattag actgttgtg agcgtaaca aatatttta 
      catttttta tttatactt aaaaactca ctccttctg 
      taacatatc gtaatttct tttcttagt taactacta 
      gataaccat cacgtaact actaataat tagttttta 
      ttataaagc acacttata ttgtgaaaa gaattattc 
      taaaagtat ataattggc catattcat tgttagaaa 
      acagcttta ttttatgca ggactgttc aaattaaaa 
      caattaatt gctacgtca gtcttctcg tacgaatgc 
      ctccctctg gaatgtgaa cgaaggttg caacagtcc 
      acttttatt gggcttaaa agcccaata gagtgatct 
      cgataacac tgaaagtga tggacaaaa aggactagt 
      taatactgc atactgcat aaaataaag cacaaacct 
      tatgtgcac ttttttaca ttttacagt ccaggcaag 
      cgacatccg aatcagttg tttaacgag cataatgtt 
      tgccaaatc gagtgagcg cagcgatta gttcacttg 
      actggactg tgatatatg acatcgcat ctcgcaaat 
      aatttcaat gtggtggca ccttcttgt gctttcaac 
      gttatgatg tccaccaca agggaacgt gagcgagat 
      ttgatagat cgtctccgt tcctttagt agctatata 
      cagaatata taaacaacg tttattgat gtctcgata 
      tttgtctgg acaacagct aatcgaatt aaaaattaa 
      caacaaatc gtatatatc ataataaca catggttac 
      tgattaccg acaagatgt agtagctct gtacaaaag 
      caagcgact tgcttcggc aagtgcgcg ccaacttga 
      tgagtaggc catatggaa acatgcgcc gcctgcacc 
      atggattca gcaggtatt acccgactg gatacatca 
      ctaataata gaataatgc aatgcacgg ctataacaa 
      cactaacct aaacgttga taagcattc gcagaatga 
      tgagatttc gtagacatc gaaattaga caaaagcca 
      cgcacagaa attgcaaag atgatttcg tacactaga 
      acattcccg cttttaaca ccctccgat cttaagaca 
      cgcagaaag aacttgcac aaaagggaa actgctctc 
      tcaatatta tctctatgg tgaagccta gataaaaca 
      gaactgtat ataccatgg tacttaaaa atggttgtt 
      cctgacata gagtaagaa gtaaatggc ttggaagaa 
      tggaataac aatctttaa ccaattttt actagaatt 
      ttggtgaaa aaacggttt caaatttgg attctgcct 
      aaaagattg ggagaattc agtggtcgc ctaaagaga 
      gtcccatct gcgtgcctg tgcgttgtg ctgaacaga 
      aaccggcta gacacgtaa aaacacaac gtaactctc 
      agtgttgta cagtgacac tatcacttg tttgatagt 
      aagttttaa aaggacaca ctagttgat tgtttttac 
      gcttcgcct aatgcctaa tgcaacagt tgtgtgtcg 
      gatattatt atgtacttg agcaaaatc tacggtacg 
      gttaaaatt gcttggaaa taatgctct gattagaga 
      tacagcaaa aaatgttgg tataataat taatggaat 
      cgtccaatc tacccaaag cgtctgaga aagagattc 
      ggctttaaa actaatatt aaagtacac aacctattc 
      tattttgtc caataaatg ttacattga ttagaatgt 
      tcatatgtc taaagagaa gtctaacat tctaacaat 
      tgtacataa taaatatct caacatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY