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Gene information
General information
TC005669 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9867650..9871919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005669
    iBeetle screen iB_08920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005669 RA
      MNSFKFPSL ALLLVLALP HTLCVYLRM SNFDLMKCS 
      YGERGSLTA TCVNATSSY FKNTPYRFD HLDETLRCV 
      NCSLHTLET GSFDISGNQ IKNLDLTNS LIETVRQKA 
      FVGLIFLQR LILANNAIK SIYPGTFTG VKKITYVDL 
      ENNSISILS DDGFLELIN LEELNLRHN EIKSIATSA 
      FNGLVHLQE LDLSYNAIG DINGVFNNL TSLRLLDLS 
      YNKISVLTG KEFDNLTSL LEIRFKFNH ITTIPASEF 
      YSMSRLRRL DLSFNAISG VRAGSFKGL HALEILDLG 
      NNAVAEVPQ KTLQSLHNL QYLNFSNNR LSIFQTGLY 
      SGLPQLRVL NFSHNVIED IEITGVFSL DSLDTLDFS 
      FNNISNVDY VRLISRLPK ISYLNLEDN LLPCGLEKE 
      MEDYFAEDN FKFILYRNE ANAVKCVQT DALPKNKHT 
      FHKDSLIRA EMGGSSANA WIFVLIGIT LVLVCVLYY 
      LQLRTYQEM RSMSVKRTT SEVQLISSD LESRDNDYL 
      KE
    CDS sequence
    • >TC005669 RA
      atgaacagt ttcaagttt ccttctctg gccctcctg 
      ctggtgttg gcccttccc cacaccctt tgtgtatac 
      cttcgaatg agcaacttt gacctaatg aaatgctcc 
      tacggtgag cgaggctct ttaaccgcc acctgcgta 
      aacgccacc tccagctac ttcaagaac accccttac 
      cgattcgac cacctggac gaaaccctc cgctgtgta 
      aactgctct ctccacact ctcgaaacc ggaagtttc 
      gatatttcc ggcaaccaa atcaaaaac ctcgaccta 
      accaacagc ctcatcgag accgtccgc cagaaagcc 
      ttcgttggt ttgattttc ctccaacgt ttgatcctc 
      gccaacaac gctattaag tccatctat cccggaacg 
      ttcaccgga gtcaagaaa atcacctac gttgacttg 
      gaaaataac tcaatcagt attttatcc gatgacggt 
      ttcctcgaa ctgatcaac ttggaggag ctgaatttg 
      cgacacaac gagattaaa agcatcgcc acgtcggct 
      ttcaacggc ttggtgcac ttgcaggag ctggatctg 
      agctacaat gccatcggt gatatcaac ggggtgttc 
      aataatttg acgagtctg cggctgttg gatctgagc 
      tataataaa atttccgtg ctgacgggg aaagagttt 
      gataatttg acgtcgctg ttggagatt cggttcaag 
      tttaatcac ataacgaca attccggcg tcagaattc 
      tactcgatg agtcgatta agacgtttg gatttatcg 
      tttaatgcc attagtggc gtaagggct gggtctttc 
      aaaggactg cacgctttg gaaatccta gatctgggc 
      aacaacgcc gtcgcggaa gtgccccag aagacgctg 
      caaagtttg cacaatctc caatacttg aacttctcc 
      aacaacagg ttatctata ttccagacg gggctttac 
      tcgggcctg ccccagcta agagtattg aatttctcg 
      cacaacgtt atcgaggat attgagatc accggagtg 
      ttctcactg gacagtctg gacacgctg gacttctcc 
      ttcaacaat atatcaaac gttgattac gtccggctg 
      atatctaga ctcccaaaa atctcctat ttgaatctg 
      gaagataac ttattgccc tgcgggctg gagaaagaa 
      atggaggac tactttgcc gaggataat ttcaagttt 
      attttgtac agaaatgag gcgaatgcc gtcaagtgt 
      gtgcaaacg gacgcgcta ccaaagaac aaacacact 
      ttccacaaa gacagtttg attagggct gagatgggc 
      ggaagcagt gcaaatgcc tggattttt gtactgatt 
      ggaatcacg ctcgtctta gtttgtgtt ctttattat 
      ctacaactt cgtacttac caagaaatg aggtctatg 
      tcggttaag cggactacg tccgaagtc caattgatt 
      tcttccgat ctggagtct agagataac gattatttg 
      aaagagtaa
    mRNA sequence
    • >TC005669 RA
      atgttctta acaaaacac gcaacaaat gagtccatt 
      ctcgtttag aggatcgtt cggaaatga tacacagat 
      tctattagc ctgttatca ctctttctt cctgctgaa 
      ttgtctgat tacacgttc ccagtgtca ttagtggtt 
      ttgtgcaac ttttgacac agtcggtgc tggctaaaa 
      tgaacagtt tcaagtttc cttctctgg ccctcctgc 
      tggtgttgg cccttcccc acacccttt gtgtatacc 
      ttcgaatga gcaactttg acctaatga aatgctcct 
      acggtgagc gaggctctt taaccgcca cctgcgtaa 
      acgccacct ccagctact tcaagaaca ccccttacc 
      gattcgacc acctggacg aaaccctcc gctgtgtaa 
      actgctctc tccacactc tcgaaaccg gaagtttcg 
      atatttccg gcaaccaaa tcaaaaacc tcgacctaa 
      ccaacagcc tcatcgaga ccgtccgcc agaaagcct 
      tcgttggtt tgattttcc tccaacgtt tgatcctcg 
      ccaacaacg ctattaagt ccatctatc ccggaacgt 
      tcaccggag tcaagaaaa tcacctacg ttgacttgg 
      aaaataact caatcagta ttttatccg atgacggtt 
      tcctcgaac tgatcaact tggaggagc tgaatttgc 
      gacacaacg agattaaaa gcatcgcca cgtcggctt 
      tcaacggct tggtgcact tgcaggagc tggatctga 
      gctacaatg ccatcggtg atatcaacg gggtgttca 
      ataatttga cgagtctgc ggctgttgg atctgagct 
      ataataaaa tttccgtgc tgacgggga aagagtttg 
      ataatttga cgtcgctgt tggagattc ggttcaagt 
      ttaatcaca taacgacaa ttccggcgt cagaattct 
      actcgatga gtcgattaa gacgtttgg atttatcgt 
      ttaatgcca ttagtggcg taagggctg ggtctttca 
      aaggactgc acgctttgg aaatcctag atctgggca 
      acaacgccg tcgcggaag tgccccaga agacgctgc 
      aaagtttgc acaatctcc aatacttga acttctcca 
      acaacaggt tatctatat tccagacgg ggctttact 
      cgggcctgc cccagctaa gagtattga atttctcgc 
      acaacgtta tcgaggata ttgagatca ccggagtgt 
      tctcactgg acagtctgg acacgctgg acttctcct 
      tcaacaata tatcaaacg ttgattacg tccggctga 
      tatctagac tcccaaaaa tctcctatt tgaatctgg 
      aagataact tattgccct gcgggctgg agaaagaaa 
      tggaggact actttgccg aggataatt tcaagttta 
      ttttgtaca gaaatgagg cgaatgccg tcaagtgtg 
      tgcaaacgg acgcgctac caaagaaca aacacactt 
      tccacaaag acagtttga ttagggctg agatgggcg 
      gaagcagtg caaatgcct ggatttttg tactgattg 
      gaatcacgc tcgtcttag tttgtgttc tttattatc 
      tacaacttc gtacttacc aagaaatga ggtctatgt 
      cggttaagc ggactacgt ccgaagtcc aattgattt 
      cttccgatc tggagtcta gagataacg attatttga 
      aagagtaac gtttgtgta cataaattc taggtttac 
      tgtgagatt ttatgataa aagccacca ctgtacgag 
      tgttttata caaaaataa taaatgtaa atgtattgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY