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Gene information
General information
TC034087 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9729892..9732708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034087
    iBeetle screen iB_08919

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands partially content darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034087 RA
      MSQDKVSLL RDPTAGVST SSDSRNYDS VDPPPHTEN 
      RAQISFVPR RLPQYVAAL TATLSALAA GAVLGWTSP 
      ILSDLQHGK FHNISVTSD QMGWIGSFV TLGGMTMCI 
      PTGFLCDLL GRKKTLLLL IAPFAVGWS LIIFAKSII 
      MLYLGRLIT GMAAGASCV AAPLYTSEI AQKEIRGTL 
      GSYFQLMVT VGIFLAYLS GKYLTSMPY TIFCACLPV 
      VFVVLFAFQ PETPAFCLR RGRYDDALK ALVKLRGPC 
      EGNESELAE IEGSLKESL ESSVSFSQT FRKKANVKA 
      LVIAFALMF FQQFSGINA VILYTSDIF ASAGSNLDA 
      NTAAIIVGA FQVVATFVS SLVIDKLGR KILLFTSAV 
      VMALSSLVL AIYFTLKCR TSIDGDILH ELGFIPIVS 
      LCLFVVVFS IGLGPIPWM ISSEIFTPE IKSIASSSA 
      GTFNWFLAF LVTKFYLQV NERVGQDST FYAFAVLSL 
      LGGAFVYFV IPETKGKTV EQVQAELER
    CDS sequence
    • >TC034087 RA
      atgagccaa gacaaagtg tctctatta cgcgaccca 
      actgccggt gtttccaca tcatcagac agcagaaac 
      tacgactct gtggaccct ccaccccac accgaaaac 
      cgtgcccaa atcagcttt gtccctcgc cgactcccc 
      caatatgtg gccgccttg acagcaact ttatcggcc 
      ctcgctgct ggtgccgtc ctgggctgg acttccccc 
      attttaagc gacctccag cacggtaaa ttccacaat 
      atttcagtc actagcgac caaatgggc tggattggc 
      tcgttcgtc actctaggt ggcatgaca atgtgcatt 
      cctactggt tttttgtgc gacttgcta ggccgtaaa 
      aaaacactt cttctcctt attgcaccg ttcgccgtt 
      ggttggtcg ctcattatt ttcgccaaa tcgataata 
      atgctgtat ttggggcgc ctgatcacc gggatggcc 
      gctggggcg tcgtgcgtg gcagccccc ttgtacacg 
      agcgaaatc gcgcagaag gaaatccgc ggcacgctt 
      ggcagctac ttccagttg atggtaact gtcggtatt 
      ttcctagcc tatttgtcc gggaaatac ctcacttca 
      atgccctat acgattttt tgtgcttgt ctcccggtt 
      gttttcgtc gttttgttt gcatttcaa ccggaaact 
      ccggcgttt tgtttacgt agggggcgc tatgacgac 
      gctctgaag gcgttggtt aagttaagg gggccctgt 
      gagggcaac gagtccgaa ttggccgaa attgaaggc 
      tcgttgaag gaaagtctc gaaagttcg gtttctttt 
      tcgcaaacg tttcgcaaa aaagccaac gttaaagcg 
      ctcgtgatt gcgtttgcg ttgatgttt ttccaacag 
      tttagtggc attaatgct gttattttg tacacgagt 
      gatattttt gcatcagct ggttcaaat cttgatgct 
      aatactgcg gcgattatc gtgggggcc tttcaggtt 
      gttgctacg ttcgtttcg tctttggtg attgataaa 
      ttggggcgg aaaattctc ctatttacc tcggccgtg 
      gttatggca ctttcaagt ctcgtcttg gcgatttat 
      ttcactttg aaatgtcga acgtccatt gatggtgat 
      attttgcac gagcttggt ttcattccg atcgtgtcg 
      ctgtgtctt tttgtggtg gttttctcc ataggttta 
      ggtcccatt ccttggatg atttcttcg gagattttt 
      acaccagaa attaaaagt atagccagt tcaagtgct 
      ggtactttc aattggttt ttagcattt ctagtgact 
      aagttttat cttcaagta aatgaacgt gtaggtcag 
      gactcgact ttttatgca tttgctgtt ttgtctctt 
      ttgggtggg gcgtttgtt tattttgtg atacctgag 
      actaaagga aaaactgtc gaacaagtc caggcagaa 
      cttgaacgt tga
    mRNA sequence
    • >TC034087 RA
      tttcaactt tcagcggtt gtttttcac ccaaaatcg 
      aaatttttt aaagtaaaa cgtaagaca agggtccca 
      caatcattc ccaatgagc caagacaaa gtgtctcta 
      ttacgcgac ccaactgcc ggtgtttcc acatcatca 
      gacagcaga aactacgac tctgtggac cctccaccc 
      cacaccgaa aaccgtgcc caaatcagc tttgtccct 
      cgccgactc ccccaatat gtggccgcc ttgacagca 
      actttatcg gccctcgct gctggtgcc gtcctgggc 
      tggacttcc cccatttta agcgacctc cagcacggt 
      aaattccac aatatttca gtcactagc gaccaaatg 
      ggctggatt ggctcgttc gtcactcta ggtggcatg 
      acaatgtgc attcctact ggttttttg tgcgacttg 
      ctaggccgt aaaaaaaca cttcttctc cttattgca 
      ccgttcgcc gttggttgg tcgctcatt attttcgcc 
      aaatcgata ataatgctg tatttgggg cgcctgatc 
      accgggatg gccgctggg gcgtcgtgc gtggcagcc 
      cccttgtac acgagcgaa atcgcgcag aaggaaatc 
      cgcggcacg cttggcagc tacttccag ttgatggta 
      actgtcggt attttccta gcctatttg tccgggaaa 
      tacctcact tcaatgccc tatacgatt ttttgtgct 
      tgtctcccg gttgttttc gtcgttttg tttgcattt 
      caaccggaa actccggcg ttttgttta cgtaggggg 
      cgctatgac gacgctctg aaggcgttg gttaagtta 
      agggggccc tgtgagggc aacgagtcc gaattggcc 
      gaaattgaa ggctcgttg aaggaaagt ctcgaaagt 
      tcggtttct ttttcgcaa acgtttcgc aaaaaagcc 
      aacgttaaa gcgctcgtg attgcgttt gcgttgatg 
      tttttccaa cagtttagt ggcattaat gctgttatt 
      ttgtacacg agtgatatt tttgcatca gctggttca 
      aatcttgat gctaatact gcggcgatt atcgtgggg 
      gcctttcag gttgttgct acgttcgtt tcgtctttg 
      gtgattgat aaattgggg cggaaaatt ctcctattt 
      acctcggcc gtggttatg gcactttca agtctcgtc 
      ttggcgatt tatttcact ttgaaatgt cgaacgtcc 
      attgatggt gatattttg cacgagctt ggtttcatt 
      ccgatcgtg tcgctgtgt ctttttgtg gtggttttc 
      tccataggt ttaggtccc attccttgg atgatttct 
      tcggagatt tttacacca gaaattaaa agtatagcc 
      agttcaagt gctggtact ttcaattgg tttttagca 
      tttctagtg actaagttt tatcttcaa gtaaatgaa 
      cgtgtaggt caggactcg actttttat gcatttgct 
      gttttgtct cttttgggt ggggcgttt gtttatttt 
      gtgatacct gagactaaa ggaaaaact gtcgaacaa 
      gtccaggca gaacttgaa cgttgaatt attgttatt 
      acacaatga atgtttaaa tactcaatg ttatgattg 
      tctcaaata tctaaatgg tgctagtta taatttcat 
      taatgacac tgttcagca aattgggac gaaattatg 
      tttctttaa tgaagaaat tttgagaaa atcgctggt 
      ttttggcgt tttgttggc actttcctg cttaggaaa 
      actgaaaaa atatttttt aataaatga aaaattact 
      tttttaact cttaaactt atgacattg atgtaaatg 
      aaaacaaat ataaaaaat aagacattt cggctgccc 
      caagctaag gcacagtat ctgcttttt acttaattt 
      ttcactgga cccaaaaat aacttttaa aaatactac 
      tcaccacta ctacttaaa tattaaatg ctattaaaa 
      agtaaatac aatttcaag tagttttca gtacaaaag 
      caattagtt aataataag ataacgaat attaacatt 
      tacgaacaa atttatgca ataacccga gacagaaat 
      ttggccaac ttaaagttc gactaaaag taatatatt 
      atctgataa tgaaattat aaaatatag atttttaca 
      cctatgtga gttattttt acttggagt acaattgac 
      tgtctctat ggtaggtat tttgtagat actttttgg 
      ggaaaggaa tattctatt ggagtaact gtttatggg 
      gaaaaaata tattaattt aaataaaaa tagctaatg 
      aatatttaa ttgaatcta agcggaaaa ataaaacaa 
      aatatgtaa aaaatatgt tggatactg tgtttttcc 
      ttaggacag ccaagttaa tttgtaaaa ttcaattaa 
      aaattctct aaatctctc taaagtctg ggtggaaat 
      gtacaactt tcactttca aactttcag taattttat 
      ttcctaatg cagttaggg gaagttaca cgtttacac 
      actttatat atatttttt tatttatat atttttttc 
      atcatttct gcaaaaact agtgtttta aacgtgata 
      tttttctga taaaatata tccctattt tttttaaca 
      aaattcttc aaattcttt tacgagtat agtgtacca 
      gttagaaaa tttttcaaa atttcaatt ggttttagt 
      cagaatata atataataa tatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY