General information | |||
TC034087 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9729892..9732708 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC034087 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08919 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034087 RA
MSQDKVSLL RDPTAGVST SSDSRNYDS VDPPPHTEN RAQISFVPR RLPQYVAAL TATLSALAA GAVLGWTSP ILSDLQHGK FHNISVTSD QMGWIGSFV TLGGMTMCI PTGFLCDLL GRKKTLLLL IAPFAVGWS LIIFAKSII MLYLGRLIT GMAAGASCV AAPLYTSEI AQKEIRGTL GSYFQLMVT VGIFLAYLS GKYLTSMPY TIFCACLPV VFVVLFAFQ PETPAFCLR RGRYDDALK ALVKLRGPC EGNESELAE IEGSLKESL ESSVSFSQT FRKKANVKA LVIAFALMF FQQFSGINA VILYTSDIF ASAGSNLDA NTAAIIVGA FQVVATFVS SLVIDKLGR KILLFTSAV VMALSSLVL AIYFTLKCR TSIDGDILH ELGFIPIVS LCLFVVVFS IGLGPIPWM ISSEIFTPE IKSIASSSA GTFNWFLAF LVTKFYLQV NERVGQDST FYAFAVLSL LGGAFVYFV IPETKGKTV EQVQAELER
- >TC034087 RA
atgagccaa gacaaagtg tctctatta cgcgaccca actgccggt gtttccaca tcatcagac agcagaaac tacgactct gtggaccct ccaccccac accgaaaac cgtgcccaa atcagcttt gtccctcgc cgactcccc caatatgtg gccgccttg acagcaact ttatcggcc ctcgctgct ggtgccgtc ctgggctgg acttccccc attttaagc gacctccag cacggtaaa ttccacaat atttcagtc actagcgac caaatgggc tggattggc tcgttcgtc actctaggt ggcatgaca atgtgcatt cctactggt tttttgtgc gacttgcta ggccgtaaa aaaacactt cttctcctt attgcaccg ttcgccgtt ggttggtcg ctcattatt ttcgccaaa tcgataata atgctgtat ttggggcgc ctgatcacc gggatggcc gctggggcg tcgtgcgtg gcagccccc ttgtacacg agcgaaatc gcgcagaag gaaatccgc ggcacgctt ggcagctac ttccagttg atggtaact gtcggtatt ttcctagcc tatttgtcc gggaaatac ctcacttca atgccctat acgattttt tgtgcttgt ctcccggtt gttttcgtc gttttgttt gcatttcaa ccggaaact ccggcgttt tgtttacgt agggggcgc tatgacgac gctctgaag gcgttggtt aagttaagg gggccctgt gagggcaac gagtccgaa ttggccgaa attgaaggc tcgttgaag gaaagtctc gaaagttcg gtttctttt tcgcaaacg tttcgcaaa aaagccaac gttaaagcg ctcgtgatt gcgtttgcg ttgatgttt ttccaacag tttagtggc attaatgct gttattttg tacacgagt gatattttt gcatcagct ggttcaaat cttgatgct aatactgcg gcgattatc gtgggggcc tttcaggtt gttgctacg ttcgtttcg tctttggtg attgataaa ttggggcgg aaaattctc ctatttacc tcggccgtg gttatggca ctttcaagt ctcgtcttg gcgatttat ttcactttg aaatgtcga acgtccatt gatggtgat attttgcac gagcttggt ttcattccg atcgtgtcg ctgtgtctt tttgtggtg gttttctcc ataggttta ggtcccatt ccttggatg atttcttcg gagattttt acaccagaa attaaaagt atagccagt tcaagtgct ggtactttc aattggttt ttagcattt ctagtgact aagttttat cttcaagta aatgaacgt gtaggtcag gactcgact ttttatgca tttgctgtt ttgtctctt ttgggtggg gcgtttgtt tattttgtg atacctgag actaaagga aaaactgtc gaacaagtc caggcagaa cttgaacgt tga
- >TC034087 RA
tttcaactt tcagcggtt gtttttcac ccaaaatcg aaatttttt aaagtaaaa cgtaagaca agggtccca caatcattc ccaatgagc caagacaaa gtgtctcta ttacgcgac ccaactgcc ggtgtttcc acatcatca gacagcaga aactacgac tctgtggac cctccaccc cacaccgaa aaccgtgcc caaatcagc tttgtccct cgccgactc ccccaatat gtggccgcc ttgacagca actttatcg gccctcgct gctggtgcc gtcctgggc tggacttcc cccatttta agcgacctc cagcacggt aaattccac aatatttca gtcactagc gaccaaatg ggctggatt ggctcgttc gtcactcta ggtggcatg acaatgtgc attcctact ggttttttg tgcgacttg ctaggccgt aaaaaaaca cttcttctc cttattgca ccgttcgcc gttggttgg tcgctcatt attttcgcc aaatcgata ataatgctg tatttgggg cgcctgatc accgggatg gccgctggg gcgtcgtgc gtggcagcc cccttgtac acgagcgaa atcgcgcag aaggaaatc cgcggcacg cttggcagc tacttccag ttgatggta actgtcggt attttccta gcctatttg tccgggaaa tacctcact tcaatgccc tatacgatt ttttgtgct tgtctcccg gttgttttc gtcgttttg tttgcattt caaccggaa actccggcg ttttgttta cgtaggggg cgctatgac gacgctctg aaggcgttg gttaagtta agggggccc tgtgagggc aacgagtcc gaattggcc gaaattgaa ggctcgttg aaggaaagt ctcgaaagt tcggtttct ttttcgcaa acgtttcgc aaaaaagcc aacgttaaa gcgctcgtg attgcgttt gcgttgatg tttttccaa cagtttagt ggcattaat gctgttatt ttgtacacg agtgatatt tttgcatca gctggttca aatcttgat gctaatact gcggcgatt atcgtgggg gcctttcag gttgttgct acgttcgtt tcgtctttg gtgattgat aaattgggg cggaaaatt ctcctattt acctcggcc gtggttatg gcactttca agtctcgtc ttggcgatt tatttcact ttgaaatgt cgaacgtcc attgatggt gatattttg cacgagctt ggtttcatt ccgatcgtg tcgctgtgt ctttttgtg gtggttttc tccataggt ttaggtccc attccttgg atgatttct tcggagatt tttacacca gaaattaaa agtatagcc agttcaagt gctggtact ttcaattgg tttttagca tttctagtg actaagttt tatcttcaa gtaaatgaa cgtgtaggt caggactcg actttttat gcatttgct gttttgtct cttttgggt ggggcgttt gtttatttt gtgatacct gagactaaa ggaaaaact gtcgaacaa gtccaggca gaacttgaa cgttgaatt attgttatt acacaatga atgtttaaa tactcaatg ttatgattg tctcaaata tctaaatgg tgctagtta taatttcat taatgacac tgttcagca aattgggac gaaattatg tttctttaa tgaagaaat tttgagaaa atcgctggt ttttggcgt tttgttggc actttcctg cttaggaaa actgaaaaa atatttttt aataaatga aaaattact tttttaact cttaaactt atgacattg atgtaaatg aaaacaaat ataaaaaat aagacattt cggctgccc caagctaag gcacagtat ctgcttttt acttaattt ttcactgga cccaaaaat aacttttaa aaatactac tcaccacta ctacttaaa tattaaatg ctattaaaa agtaaatac aatttcaag tagttttca gtacaaaag caattagtt aataataag ataacgaat attaacatt tacgaacaa atttatgca ataacccga gacagaaat ttggccaac ttaaagttc gactaaaag taatatatt atctgataa tgaaattat aaaatatag atttttaca cctatgtga gttattttt acttggagt acaattgac tgtctctat ggtaggtat tttgtagat actttttgg ggaaaggaa tattctatt ggagtaact gtttatggg gaaaaaata tattaattt aaataaaaa tagctaatg aatatttaa ttgaatcta agcggaaaa ataaaacaa aatatgtaa aaaatatgt tggatactg tgtttttcc ttaggacag ccaagttaa tttgtaaaa ttcaattaa aaattctct aaatctctc taaagtctg ggtggaaat gtacaactt tcactttca aactttcag taattttat ttcctaatg cagttaggg gaagttaca cgtttacac actttatat atatttttt tatttatat atttttttc atcatttct gcaaaaact agtgtttta aacgtgata tttttctga taaaatata tccctattt tttttaaca aaattcttc aaattcttt tacgagtat agtgtacca gttagaaaa tttttcaaa atttcaatt ggttttagt cagaatata atataataa tatta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
