Status
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Gene information
General information
TC034830 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7060884..7062775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034830
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08918

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034830 RA
      MLLVLLAIA TLIYFYWKI IYPHKYWTR KGVPQSKPV 
      FLFGDIWPM IIRKYSAPE LVDEFYKID QNARYCGVY 
      QFLSPGLIL RDPELIKEI TVKDFDHFV DRRVFVKEN 
      VDPVFGRSL LSLDGEKWR NMRAAVSPV FTSSKMKYM 
      FPSISQCGE DFANYFLKQ NKDLLELEM KESITKFTN 
      DVIANTVFG FTCNSLENP NNEFYKMGD EVGNMTSMR 
      TRIVFLMNA LAPKILSFF KIYLFSPRI YTFFRQVVN 
      ENISSREKH GVTRPDMID LLVKAKNNS LKNEEADDL 
      PEAGFTTVK DTENGKKSR KYQITDEDI VAQALLFFF 
      AGFDSVAGV ISYMCYELA VAQDAQEKL RQEVDETRE 
      KCGGKISYE ELMKMKYMH MAVSETLRK WPTAIASDR 
      VCVKPYTIQ PKLPNEKPL HLKVGDTVN IPIYAIQRD 
      PQYFPEPDR FIPERFSEE NKSKIVPYT YMSFGTGPR 
      SCIGYRFAL LEMKILMYH LISNFEIVP VAKTQIPIR 
      FNPKAVNMT AEKGFWVGL KRRVKV
    CDS sequence
    • >TC034830 RA
      atgttgttg gtgttgtta gctatcgct acccttatt 
      tatttctat tggaaaata atctatcct cataagtac 
      tggacaagg aaaggtgtg ccacaaagt aaacccgtt 
      tttttattt ggggatata tggcccatg attatacgc 
      aaatactca gcgcctgaa ttagtcgac gagttttat 
      aaaatcgac caaaatgct aggtattgt ggtgtttac 
      caatttctt tcaccaggt cttatattg agagacccc 
      gagctgatc aaagaaata acagttaaa gacttcgat 
      catttcgtt gaccgtcga gtttttgtc aaagaaaac 
      gttgatcct gtttttgga cgaagtttg ttgtctctt 
      gatggcgaa aaatggcgt aatatgaga gccgctgtt 
      agtcctgtc ttcaccagc agcaaaatg aaatacatg 
      tttccgtca atatcacaa tgtggggaa gactttgca 
      aattacttt ttaaaacaa aacaaagat ttgctcgaa 
      ttggaaatg aaagaatcc atcacgaaa ttcactaac 
      gatgttatc gcaaacacc gttttcgga ttcacttgc 
      aactctttg gaaaaccca aacaacgaa ttttacaaa 
      atgggagac gaagtcggt aacatgacc tccatgagg 
      accaggatt gtgtttctc atgaacgcc ttggcgcca 
      aaaatactt tcatttttt aaaatttat cttttctct 
      ccacgaatt tacacgttt ttccggcaa gtggtcaac 
      gagaatata tcaagccgg gaaaagcac ggagtcaca 
      agacctgac atgattgat ttgttagtc aaagcgaaa 
      aataatagc ttgaaaaat gaagaagcg gacgatttg 
      cctgaagcc ggctttacg acagttaaa gacaccgaa 
      aacgggaag aaaagtcga aaataccaa ataacagac 
      gaagatata gttgcccaa gcccttctt ttcttcttc 
      gccggtttc gacagcgtg gcgggagtt ataagctac 
      atgtgttac gaattagcg gttgcacaa gacgctcag 
      gaaaaactg cgacaagaa gtcgatgaa actcgggaa 
      aagtgcggt gggaaaatt agttacgag gaattgatg 
      aagatgaaa tatatgcat atggctgtt tcagaaact 
      ttgcggaag tggccaacc gccatcgcc tcggatcgt 
      gtctgcgtt aagccctac accatccaa ccgaaactt 
      ccaaacgaa aaaccctta catttgaaa gtgggtgat 
      actgtaaat attcccatt tatgccatt cagagagat 
      cctcagtac ttcccagaa ccagatcgg tttattccg 
      gaacgtttc agcgaagaa aataaatca aaaattgtt 
      ccgtacacc tacatgtct tttgggact ggcccaagg 
      agttgtatc ggatacaga tttgctctc ttggaaatg 
      aaaattcta atgtatcat ttgatctca aattttgaa 
      atagttcca gttgccaaa acacaaatt ccgattagg 
      ttcaacccc aaagctgta aatatgact gcggaaaaa 
      ggattttgg gtgggactc aagcgacgt gtaaaagtc 
      tag
    mRNA sequence
    • >TC034830 RA
      ttaattgtc tatgaatag tcaacaaaa tgttgttgg 
      tgttgttag ctatcgcta cccttattt atttctatt 
      ggaaaataa tctatcctc ataagtact ggacaagga 
      aaggtgtgc cacaaagta aacccgttt ttttatttg 
      gggatatat ggcccatga ttatacgca aatactcag 
      cgcctgaat tagtcgacg agttttata aaatcgacc 
      aaaatgcta ggtattgtg gtgtttacc aatttcttt 
      caccaggtc ttatattga gagaccccg agctgatca 
      aagaaataa cagttaaag acttcgatc atttcgttg 
      accgtcgag tttttgtca aagaaaacg ttgatcctg 
      tttttggac gaagtttgt tgtctcttg atggcgaaa 
      aatggcgta atatgagag ccgctgtta gtcctgtct 
      tcaccagca gcaaaatga aatacatgt ttccgtcaa 
      tatcacaat gtggggaag actttgcaa attactttt 
      taaaacaaa acaaagatt tgctcgaat tggaaatga 
      aagaatcca tcacgaaat tcactaacg atgttatcg 
      caaacaccg ttttcggat tcacttgca actctttgg 
      aaaacccaa acaacgaat tttacaaaa tgggagacg 
      aagtcggta acatgacct ccatgagga ccaggattg 
      tgtttctca tgaacgcct tggcgccaa aaatacttt 
      catttttta aaatttatc ttttctctc cacgaattt 
      acacgtttt tccggcaag tggtcaacg agaatatat 
      caagccggg aaaagcacg gagtcacaa gacctgaca 
      tgattgatt tgttagtca aagcgaaaa ataatagct 
      tgaaaaatg aagaagcgg acgatttgc ctgaagccg 
      gctttacga cagttaaag acaccgaaa acgggaaga 
      aaagtcgaa aataccaaa taacagacg aagatatag 
      ttgcccaag cccttcttt tcttcttcg ccggtttcg 
      acagcgtgg cgggagtta taagctaca tgtgttacg 
      aattagcgg ttgcacaag acgctcagg aaaaactgc 
      gacaagaag tcgatgaaa ctcgggaaa agtgcggtg 
      ggaaaatta gttacgagg aattgatga agatgaaat 
      atatgcata tggctgttt cagaaactt tgcggaagt 
      ggccaaccg ccatcgcct cggatcgtg tctgcgtta 
      agccctaca ccatccaac cgaaacttc caaacgaaa 
      aacccttac atttgaaag tgggtgata ctgtaaata 
      ttcccattt atgccattc agagagatc ctcagtact 
      tcccagaac cagatcggt ttattccgg aacgtttca 
      gcgaagaaa ataaatcaa aaattgttc cgtacacct 
      acatgtctt ttgggactg gcccaagga gttgtatcg 
      gatacagat ttgctctct tggaaatga aaattctaa 
      tgtatcatt tgatctcaa attttgaaa tagttccag 
      ttgccaaaa cacaaattc cgattaggt tcaacccca 
      aagctgtaa atatgactg cggaaaaag gattttggg 
      tgggactca agcgacgtg taaaagtct agtctagat 
      aaacgaaac tcttttctc gtactgtaa atataatta 
      actattttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY