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Gene information
General information
TC005760 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8188406..8191439
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005760
    iBeetle screen iB_08913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005760 RA
      MSGHAVVVW EYETRPGYW KPYSPAVTQ HLERANAKQ 
      LTRVLLSDA DPTLFNFYV NLRTLTQVQ EETDEVVKV 
      RRKCYPPSS PAGKGAKWE CVDPENGTD WHPFDMDIQ 
      CLIEEAWAN GDQNIDMSK THLGFPYLI NFSNLTQRW 
      LTNGHVRSV RRVKQAPYP LVKVKLEEM TSVTGRREI 
      AKSAKNVTQ PVQKTIGSS AALNVVETK RSANKKRSH 
      KPKNGNETS TTNLARAIL NNLNIFSNK SSSNNFTSN 
      SENRTHKES LLDADSSST KSGRRPSLD TVSTYLSQE 
      SRESQTTVS TTDLLQCSS EDDNIDHDL LSIVGLDPA 
      SAIISKYVR VSQPCEWAP RQPCPTCRQ PLRPTLVVV 
      ALPCNHVLH LDCLNYSLT EQQENGAHL HIDCAICGC 
      VYGEKHGNQ PPGIMEWGF VDKRLPGFQ NYRTIQIVY 
      NIQSGIQGP DHPHPGKEY YAVGFPRVA YLPDCPKGQ 
      KVLRLLNIA WQRKLIFTI SRSNTTGCE DVVAWNIPH 
      KTEIGPSNS AHSYPDSGY LTRVLRELE ALGVVEEDG 
      R
    CDS sequence
    • >TC005760 RA
      atgtcgggc cacgctgtg gtcgtatgg gaatatgag 
      actagaccc ggctattgg aagccctac agccctgcg 
      gtgacccag catttagag cgagcaaat gccaaacaa 
      ctgactcgc gtgctttta tctgacgcc gacccgacc 
      ttgttcaac ttttatgtc aatttgcgc actttgaca 
      caagtacaa gaggagaca gatgaggtt gtcaaagtg 
      cgtcgcaag tgttacccg ccttcgtct ccggccgga 
      aaaggggcc aagtgggaa tgtgtcgat ccggaaaat 
      ggcaccgat tggcacccc tttgatatg gacattcag 
      tgccttatc gaggaggct tgggctaat ggggaccaa 
      aacatcgac atgagtaaa actcactta ggttttccg 
      tatttgatt aattttagt aacttgaca caaagatgg 
      ctgaccaac ggccatgtt cgaagtgtc agacgtgtc 
      aaacaagcg ccatatccg ttagttaag gttaagtta 
      gaagaaatg acgtctgtg accggtcga cgcgaaatt 
      gcaaaaagt gccaaaaat gtcacacaa ccagtacaa 
      aaaacaatt ggtagtagt gccgcctta aacgtcgtc 
      gaaacgaaa cgaagtgcc aataaaaaa cgatcacac 
      aagccaaag aacgggaat gagaccagt actaccaac 
      ctagctaga gcaatatta aataattta aatatattt 
      agcaataaa tcgagtagt aataatttc acctcgaat 
      tctgaaaac cgtactcat aaagaatca cttttggac 
      gcggacagc agtagtaca aaatcaggc cgaaggccg 
      agtcttgac actgtttcg acctattta agtcaagag 
      agccgtgaa agccaaact acagtttcc accactgat 
      ttgctgcaa tgtagcagc gaggacgac aatatcgat 
      cacgaccta ctctcgata gttggtttg gacccggct 
      agtgcaata atctcgaaa tacgtgcgg gtgtcccaa 
      ccgtgcgag tgggccccc agacagccc tgccccacg 
      tgccggcag cccctgcgc cccacgtta gtcgtggtg 
      gcgttgccc tgcaaccac gtgttgcac ctcgactgt 
      cttaactac tccctgacc gaacaacag gaaaatggg 
      gcacatttg catattgat tgtgcgatt tgtggctgc 
      gtctacgga gagaagcac gggaatcag ccccccggc 
      atcatggag tggggcttc gtcgataag cgactcccc 
      ggcttccag aactaccgc acgattcaa atcgtttat 
      aatattcag tcggggatt caagggccg gaccacccc 
      cacccaggg aaggagtac tacgctgtg gggttcccc 
      cgcgtggcc tatctgccc gattgtccc aaaggacag 
      aaagtttta cggttattg aatatcgca tggcagcgg 
      aagttgatt tttacgatc agtaggtcc aacacaaca 
      ggttgcgag gatgtggtc gcgtggaat ataccgcat 
      aaaaccgaa attgggccg agtaatagt gcgcatagt 
      taccccgat tcggggtac ttgacgagg gttttgagg 
      gagttggag gcgttgggt gttgtggag gaggacggg 
      agatga
    mRNA sequence
    • >TC005760 RA
      tctgatgac gtaacaaat ggtaatggt tgatggttt 
      gttaacaag ttgtcacaa gtgctcatt ttttgttta 
      caaaataaa atcccgtcc aattcgctt tttcctgaa 
      ttgagttta tgttgttat cagcgttat ctaatcaca 
      gaaaacaaa tgagtgtag ttttggcgc agtttcctg 
      tgtttaaaa aacaattga agttggggt catgatgtt 
      aattaaatg attgtttta acagacttg gaagcgtcc 
      aaaacagat gattttgcc gattttcgg ccaaaatgt 
      cgggccacg ctgtggtcg tatgggaat atgagacta 
      gacccggct attggaagc cctacagcc ctgcggtga 
      cccagcatt tagagcgag caaatgcca aacaactga 
      ctcgcgtgc ttttatctg acgccgacc cgaccttgt 
      tcaactttt atgtcaatt tgcgcactt tgacacaag 
      tacaagagg agacagatg aggttgtca aagtgcgtc 
      gcaagtgtt acccgcctt cgtctccgg ccggaaaag 
      gggccaagt gggaatgtg tcgatccgg aaaatggca 
      ccgattggc acccctttg atatggaca ttcagtgcc 
      ttatcgagg aggcttggg ctaatgggg accaaaaca 
      tcgacatga gtaaaactc acttaggtt ttccgtatt 
      tgattaatt ttagtaact tgacacaaa gatggctga 
      ccaacggcc atgttcgaa gtgtcagac gtgtcaaac 
      aagcgccat atccgttag ttaaggtta agttagaag 
      aaatgacgt ctgtgaccg gtcgacgcg aaattgcaa 
      aaagtgcca aaaatgtca cacaaccag tacaaaaaa 
      caattggta gtagtgccg ccttaaacg tcgtcgaaa 
      cgaaacgaa gtgccaata aaaaacgat cacacaagc 
      caaagaacg ggaatgaga ccagtacta ccaacctag 
      ctagagcaa tattaaata atttaaata tatttagca 
      ataaatcga gtagtaata atttcacct cgaattctg 
      aaaaccgta ctcataaag aatcacttt tggacgcgg 
      acagcagta gtacaaaat caggccgaa ggccgagtc 
      ttgacactg tttcgacct atttaagtc aagagagcc 
      gtgaaagcc aaactacag tttccacca ctgatttgc 
      tgcaatgta gcagcgagg acgacaata tcgatcacg 
      acctactct cgatagttg gtttggacc cggctagtg 
      caataatct cgaaatacg tgcgggtgt cccaaccgt 
      gcgagtggg cccccagac agccctgcc ccacgtgcc 
      ggcagcccc tgcgcccca cgttagtcg tggtggcgt 
      tgccctgca accacgtgt tgcacctcg actgtctta 
      actactccc tgaccgaac aacaggaaa atggggcac 
      atttgcata ttgattgtg cgatttgtg gctgcgtct 
      acggagaga agcacggga atcagcccc ccggcatca 
      tggagtggg gcttcgtcg ataagcgac tccccggct 
      tccagaact accgcacga ttcaaatcg tttataata 
      ttcagtcgg ggattcaag ggccggacc acccccacc 
      cagggaagg agtactacg ctgtggggt tcccccgcg 
      tggcctatc tgcccgatt gtcccaaag gacagaaag 
      ttttacggt tattgaata tcgcatggc agcggaagt 
      tgattttta cgatcagta ggtccaaca caacaggtt 
      gcgaggatg tggtcgcgt ggaatatac cgcataaaa 
      ccgaaattg ggccgagta atagtgcgc atagttacc 
      ccgattcgg ggtacttga cgagggttt tgagggagt 
      tggaggcgt tgggtgttg tggaggagg acgggagat 
      gaggtttga ttagttagg ttaagttgg aagtactgt 
      taatatacg cttatgcaa tatttcgaa atatttaca 
      aaaaatgta cagtatttg atacaatgt gtcctctat 
      tgaaaaata tattttata aataaagta atactatta 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY