General information | |||
TC005760 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 8188406..8191439 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC005760 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08913 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005760 RA
MSGHAVVVW EYETRPGYW KPYSPAVTQ HLERANAKQ LTRVLLSDA DPTLFNFYV NLRTLTQVQ EETDEVVKV RRKCYPPSS PAGKGAKWE CVDPENGTD WHPFDMDIQ CLIEEAWAN GDQNIDMSK THLGFPYLI NFSNLTQRW LTNGHVRSV RRVKQAPYP LVKVKLEEM TSVTGRREI AKSAKNVTQ PVQKTIGSS AALNVVETK RSANKKRSH KPKNGNETS TTNLARAIL NNLNIFSNK SSSNNFTSN SENRTHKES LLDADSSST KSGRRPSLD TVSTYLSQE SRESQTTVS TTDLLQCSS EDDNIDHDL LSIVGLDPA SAIISKYVR VSQPCEWAP RQPCPTCRQ PLRPTLVVV ALPCNHVLH LDCLNYSLT EQQENGAHL HIDCAICGC VYGEKHGNQ PPGIMEWGF VDKRLPGFQ NYRTIQIVY NIQSGIQGP DHPHPGKEY YAVGFPRVA YLPDCPKGQ KVLRLLNIA WQRKLIFTI SRSNTTGCE DVVAWNIPH KTEIGPSNS AHSYPDSGY LTRVLRELE ALGVVEEDG R
- >TC005760 RA
atgtcgggc cacgctgtg gtcgtatgg gaatatgag actagaccc ggctattgg aagccctac agccctgcg gtgacccag catttagag cgagcaaat gccaaacaa ctgactcgc gtgctttta tctgacgcc gacccgacc ttgttcaac ttttatgtc aatttgcgc actttgaca caagtacaa gaggagaca gatgaggtt gtcaaagtg cgtcgcaag tgttacccg ccttcgtct ccggccgga aaaggggcc aagtgggaa tgtgtcgat ccggaaaat ggcaccgat tggcacccc tttgatatg gacattcag tgccttatc gaggaggct tgggctaat ggggaccaa aacatcgac atgagtaaa actcactta ggttttccg tatttgatt aattttagt aacttgaca caaagatgg ctgaccaac ggccatgtt cgaagtgtc agacgtgtc aaacaagcg ccatatccg ttagttaag gttaagtta gaagaaatg acgtctgtg accggtcga cgcgaaatt gcaaaaagt gccaaaaat gtcacacaa ccagtacaa aaaacaatt ggtagtagt gccgcctta aacgtcgtc gaaacgaaa cgaagtgcc aataaaaaa cgatcacac aagccaaag aacgggaat gagaccagt actaccaac ctagctaga gcaatatta aataattta aatatattt agcaataaa tcgagtagt aataatttc acctcgaat tctgaaaac cgtactcat aaagaatca cttttggac gcggacagc agtagtaca aaatcaggc cgaaggccg agtcttgac actgtttcg acctattta agtcaagag agccgtgaa agccaaact acagtttcc accactgat ttgctgcaa tgtagcagc gaggacgac aatatcgat cacgaccta ctctcgata gttggtttg gacccggct agtgcaata atctcgaaa tacgtgcgg gtgtcccaa ccgtgcgag tgggccccc agacagccc tgccccacg tgccggcag cccctgcgc cccacgtta gtcgtggtg gcgttgccc tgcaaccac gtgttgcac ctcgactgt cttaactac tccctgacc gaacaacag gaaaatggg gcacatttg catattgat tgtgcgatt tgtggctgc gtctacgga gagaagcac gggaatcag ccccccggc atcatggag tggggcttc gtcgataag cgactcccc ggcttccag aactaccgc acgattcaa atcgtttat aatattcag tcggggatt caagggccg gaccacccc cacccaggg aaggagtac tacgctgtg gggttcccc cgcgtggcc tatctgccc gattgtccc aaaggacag aaagtttta cggttattg aatatcgca tggcagcgg aagttgatt tttacgatc agtaggtcc aacacaaca ggttgcgag gatgtggtc gcgtggaat ataccgcat aaaaccgaa attgggccg agtaatagt gcgcatagt taccccgat tcggggtac ttgacgagg gttttgagg gagttggag gcgttgggt gttgtggag gaggacggg agatga
- >TC005760 RA
tctgatgac gtaacaaat ggtaatggt tgatggttt gttaacaag ttgtcacaa gtgctcatt ttttgttta caaaataaa atcccgtcc aattcgctt tttcctgaa ttgagttta tgttgttat cagcgttat ctaatcaca gaaaacaaa tgagtgtag ttttggcgc agtttcctg tgtttaaaa aacaattga agttggggt catgatgtt aattaaatg attgtttta acagacttg gaagcgtcc aaaacagat gattttgcc gattttcgg ccaaaatgt cgggccacg ctgtggtcg tatgggaat atgagacta gacccggct attggaagc cctacagcc ctgcggtga cccagcatt tagagcgag caaatgcca aacaactga ctcgcgtgc ttttatctg acgccgacc cgaccttgt tcaactttt atgtcaatt tgcgcactt tgacacaag tacaagagg agacagatg aggttgtca aagtgcgtc gcaagtgtt acccgcctt cgtctccgg ccggaaaag gggccaagt gggaatgtg tcgatccgg aaaatggca ccgattggc acccctttg atatggaca ttcagtgcc ttatcgagg aggcttggg ctaatgggg accaaaaca tcgacatga gtaaaactc acttaggtt ttccgtatt tgattaatt ttagtaact tgacacaaa gatggctga ccaacggcc atgttcgaa gtgtcagac gtgtcaaac aagcgccat atccgttag ttaaggtta agttagaag aaatgacgt ctgtgaccg gtcgacgcg aaattgcaa aaagtgcca aaaatgtca cacaaccag tacaaaaaa caattggta gtagtgccg ccttaaacg tcgtcgaaa cgaaacgaa gtgccaata aaaaacgat cacacaagc caaagaacg ggaatgaga ccagtacta ccaacctag ctagagcaa tattaaata atttaaata tatttagca ataaatcga gtagtaata atttcacct cgaattctg aaaaccgta ctcataaag aatcacttt tggacgcgg acagcagta gtacaaaat caggccgaa ggccgagtc ttgacactg tttcgacct atttaagtc aagagagcc gtgaaagcc aaactacag tttccacca ctgatttgc tgcaatgta gcagcgagg acgacaata tcgatcacg acctactct cgatagttg gtttggacc cggctagtg caataatct cgaaatacg tgcgggtgt cccaaccgt gcgagtggg cccccagac agccctgcc ccacgtgcc ggcagcccc tgcgcccca cgttagtcg tggtggcgt tgccctgca accacgtgt tgcacctcg actgtctta actactccc tgaccgaac aacaggaaa atggggcac atttgcata ttgattgtg cgatttgtg gctgcgtct acggagaga agcacggga atcagcccc ccggcatca tggagtggg gcttcgtcg ataagcgac tccccggct tccagaact accgcacga ttcaaatcg tttataata ttcagtcgg ggattcaag ggccggacc acccccacc cagggaagg agtactacg ctgtggggt tcccccgcg tggcctatc tgcccgatt gtcccaaag gacagaaag ttttacggt tattgaata tcgcatggc agcggaagt tgattttta cgatcagta ggtccaaca caacaggtt gcgaggatg tggtcgcgt ggaatatac cgcataaaa ccgaaattg ggccgagta atagtgcgc atagttacc ccgattcgg ggtacttga cgagggttt tgagggagt tggaggcgt tgggtgttg tggaggagg acgggagat gaggtttga ttagttagg ttaagttgg aagtactgt taatatacg cttatgcaa tatttcgaa atatttaca aaaaatgta cagtatttg atacaatgt gtcctctat tgaaaaata tattttata aataaagta atactatta ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
