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Gene information
General information
TC005767 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7921339..7936549
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005767
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08910

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    yolk sometimes deposition irregular - Number of animals showing the phenotype: 2

    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    mandible sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    maxilla sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005767 RA
      MSELSQEEM RRRRLARLA VLDNVLPSG SVSPPVTPV 
      SQSPAAQHS PPTTPEKDF EGVFQVPSK PIDMPSGSK 
      SRRPPPQRS DSETSSIHM EVDEASGCA DKAGANTDI 
      DSGFENMEV DETDVQKKD APRQRTSSS ATEITEEQL 
      LATVLRILH STLEQPSES RIYLPQTAT DVKTHPQTS 
      IRDIISNAL MEIIVQISQ GNNPFKDLI PSNDTADTS 
      SINSLSPSP SPSHTCPIP TLPIKRVEV AETPLNLAL 
      NYLMDCYNR VSVEERNHP KRSSIPPLS DVLTEVRAQ 
      LVHYTTLLL QGFIIANDE LYKFGRSPL LSPILQQTL 
      PRGFLTELV TRTHTNTSL FSSVFSPLL QGLYRMMQN 
      ASIVGEEHR MPIQTLFEL ADIRCGSRP ICTLITKQV 
      QFMLEPCTP AQGREVVRT SFLGPFLSV SVFAEDEPK 
      VAEKFFSGN SSSDKSLNH TLQLELENT RNLQHRIFH 
      YLLANPESR DGCLNYLAK VLKYNEKRA QLQMEERSL 
      AGDGFMLNL LSVLQMLSM KIKLDKMDF LYPFHSESL 
      ICIKNDTRL KYTSQDVAT WLESLEKTH QFQTPNFST 
      ICWFLTLHC HHLALLPAL QKYQRRIRA IRDLQKLLD 
      ETVAAEAQW RNTPFANRN KQFIKRWKQ QLKKLNKSK 
      ACADAGLLD KNLMRRALI FYTSVAQYL LSLMTNMAP 
      GSPVPSLPL PPNVPEAFS ALPEWYVED IAEFLLFAL 
      PYFPTVITE NMEDSLITW LLVTICSSN MVKNPYLVA 
      KLVEVVFII IPTFQPRCE MLYDRFMSH EISRSVLPS 
      ALMKFYTDV ETTGSSSEF YDKFSIRYH ISLIIKGMW 
      NSAIHRQTL VNESKSGKQ FVKFVNMLM NDTTFLLDE 
      SLESLKRIH EVQELISDE EKWSKMNSE QQQSRMRQL 
      TADERQCRS YLTLARETV DMFHYLTVD IKEPFLRPE 
      LVDRLASML NFNLQQLCG PKCKNLKVR NPDKYGWEP 
      RRLLSQLVD IYLHLDCEE FAAALAGDE RSFRKDLFD 
      DAAARLERL SIKTPVEIE RFKALADKA YHVYQNNQK 
      SDDWMSDAP DEFKDPLMD TLMTDPVLL PSGQVMDRS 
      VIMRHLLNS STDPFNRQP LTEDMLQPV NELKERIRI 
      WKSEKTRSA N
    CDS sequence
    • >TC005767 RA
      atgagcgaa ctgtcgcaa gaagagatg cgtcgacgg 
      cgactggcc cgcctggca gtcctggac aacgtcttg 
      ccctccggc tcggtctcg ccgcccgtg acccccgtc 
      tcgcagtcg ccggcggcg cagcacagc cccccgacc 
      acccccgag aaggacttc gagggggtg ttccaggtg 
      cccagcaag cccatcgac atgccctcc ggctccaag 
      agccgcagg ccgccgccc cagcggagc gactcggag 
      acgagctcc atacacatg gaggtggac gaggccagc 
      gggtgcgcc gacaaggcg ggggccaac acggacatc 
      gactcgggg ttcgaaaat atggaggtc gacgagacc 
      gacgtccag aagaaagac gcccctcgt caacgaaca 
      tcatcctca gcgaccgaa atcaccgaa gaacaactc 
      ctagcaacg gtcctccgc atcctccat tccaccctc 
      gaacaacca tccgaaagt cgcatttat ctccctcaa 
      accgccacc gacgtcaaa acccaccct caaacctca 
      atccgcgac atcatctcc aacgcactg atggaaatc 
      atcgtccaa atctcacaa ggcaataac cccttcaaa 
      gacctgatc ccgagcaac gacacagcc gacaccagc 
      agcataaac tcgctaagt ccctccccc agccctagc 
      cacacctgc cccataccc accctgcca atcaaacga 
      gtggaagtg gcggaaacg cccctaaac ctagcactg 
      aattaccta atggattgc tacaatcgt gtgagcgtc 
      gaggaacga aaccacccg aagcgatcg agtattccc 
      ccgttaagt gacgtttta acggaagtt cgggcacaa 
      ctggtgcac tacacaacg ttgttactc caaggtttt 
      atcatagcc aatgacgag ttgtacaaa ttcgggcgt 
      tcgcccctt ttgagcccc attttgcag cagacgctc 
      ccgcggggg ttcctcacc gagctggtg acccgaacc 
      cacacgaac acgtcgttg ttctcgtcg gttttctcg 
      ccactgctt cagggactt taccgaatg atgcagaac 
      gcgagtatc gtgggcgag gagcaccga atgccgatc 
      cagacattg ttcgagttg gccgatatt cggtgcggc 
      agccgcccc atctgtacg ctaatcacg aaacaagtg 
      caatttatg ctggagccg tgtacgcct gcgcaaggc 
      cgcgaagtt gtccgaacg tcgtttctc gggccgttt 
      ctgtcggtg tcggtgttc gccgaagac gagccgaaa 
      gtggccgag aagttcttc tcggggaat tcgtcgagt 
      gataagagt ttgaatcat acgctgcag ctggagctg 
      gagaatacg cggaatttg caacataga atttttcat 
      tatttgttg gcgaatccg gagagtcgt gatgggtgt 
      ttgaattat ttggcgaag gttttgaag tataatgag 
      aagagggcg cagctgcag atggaggag aggtcgctg 
      gctggggac gggtttatg ttgaatttg ttaagtgtt 
      ttgcaaatg ttgtcgatg aagattaag ttggataag 
      atggatttt ttgtatccg tttcattcc gaaagtttg 
      atttgtatt aagaatgat acgaggttg aagtatacg 
      tcgcaggat gtcgccacg tggctggag agtttggaa 
      aaaacccac caattccaa acgcccaac ttttcgaca 
      atttgttgg ttcttgaca ctccactgc catcacctc 
      gccctcctt cccgcactc caaaaatac caacgtcgc 
      attcgcgcc attcgtgat ctacaaaaa ctcctcgac 
      gaaacggtg gctgcggaa gcacagtgg cgtaacaca 
      ccgttcgcc aatcgcaat aaacagttt atcaaacgg 
      tggaaacaa caattgaaa aaattgaac aaatctaaa 
      gcgtgcgct gatgccgga ctactagat aagaatcta 
      atgcgacgc gctttgatt ttttacacc tcagtcgct 
      cagtattta ctgagtctg atgacaaat atggcgccg 
      gggagtccc gtgccgagt ctaccactg ccgccgaac 
      gtacccgaa gcattctcg gcgcttccc gaatggtac 
      gtcgaagat atagccgag tttcttcta tttgcctta 
      ccatatttc ccgaccgtc atcacggag aacatggaa 
      gattcccta atcacgtgg ttgctagtg acaatttgt 
      tcctccaac atggtcaaa aatccctac ttggtggcc 
      aaactggtc gaagtcgtc ttcataata atcccaacg 
      tttcaaccg cgttgcgaa atgttgtac gaccgtttc 
      atgtcgcac gaaatttca cgaagtgtg ctacccagt 
      gccctgatg aagttctac accgatgtc gaaacgacc 
      ggctccagt tcggaattt tacgacaag ttttcaatc 
      cgttaccat atcagtctc ataattaaa ggcatgtgg 
      aactcggca atacatcgc caaacgctc gtcaacgaa 
      tcaaaatca ggaaaacag ttcgtcaaa ttcgtcaat 
      atgctaatg aacgacacc actttcctc ctcgacgag 
      agtctcgaa agtttgaaa cgcatccac gaagtgcaa 
      gagttgata agcgatgag gagaaatgg tcgaaaatg 
      aattcggaa cagcagcaa agtcggatg agacaattg 
      acagccgat gagagacag tgtcgctcc tacttgact 
      ctagctcgt gaaactgtc gatatgttt cactactta 
      acggttgat attaaggag ccgtttttg cgaccggaa 
      cttgttgat cgtttagcg tcgatgctt aattttaat 
      ttgcaacaa ttgtgtggc ccgaaatgt aaaaatttg 
      aaagtgaga aatccggat aagtatggc tgggagccg 
      cgacggttg ttgagtcaa ttggttgat atttatctg 
      catttggac tgcgaggag ttcgccgcc gctttggcc 
      ggcgatgag agatctttc cggaaagat ttgtttgac 
      gacgcagcg gcgcgatta gagcgcctg tcaatcaaa 
      actccggtt gagatcgaa cggtttaag gccttggcc 
      gataaggcg tatcacgtg tatcaaaat aaccagaaa 
      agtgatgac tggatgagc gacgcccct gacgaattc 
      aaggatccc ttaatggac actttgatg actgatcct 
      gtgttgttg cccagtggg caggtgatg gacaggtcc 
      gtcattatg aggcatttg ttgaatagc agtacggac 
      ccgtttaat cggcaacca ttgactgaa gatatgctg 
      cagccagtg aatgagttg aaggagcgc attaggata 
      tggaaatca gaaaagaca cgttcggca aattaa
    mRNA sequence
    • >TC005767 RA
      acgcaaaat tgtcttttt gggaaaatc atatttagc 
      gcccttgtg ctgtataat gtgttgcct gaaaacggg 
      ctgattttt gacaggtgt gccgaactt tgtaaattt 
      tcccgatta ataaaatcc ggaaaaagg tgaaaatac 
      gtcgtaaat gcagatgaa aacaaaggg atttggcgg 
      ccaagtgca aattttaaa atagaaatc atttttccc 
      ccagtctgt tgaaaataa agtgcgtgc gcaatcacg 
      tgaagcgag acaaacaat cgccggtgt gataagagg 
      aagcgagac gaggcgatt cctcagtta tcgagttgt 
      taggttaga ctgtgacaa aacaaagat gttgagtgt 
      gatttcgca aatattcta atgtcaaat ttgtaactc 
      agctgttga gggtgattg acttttcat tttgatcaa 
      aaccgtgaa ttacccgtc actattcgt ccgaaaccg 
      ccctcaaaa agctccaaa cagccccac acaagacaa 
      tgagcgaac tgtcgcaag aagagatgc gtcgacggc 
      gactggccc gcctggcag tcctggaca acgtcttgc 
      cctccggct cggtctcgc cgcccgtga cccccgtct 
      cgcagtcgc cggcggcgc agcacagcc ccccgacca 
      cccccgaga aggacttcg agggggtgt tccaggtgc 
      ccagcaagc ccatcgaca tgccctccg gctccaaga 
      gccgcaggc cgccgcccc agcggagcg actcggaga 
      cgagctcca tacacatgg aggtggacg aggccagcg 
      ggtgcgccg acaaggcgg gggccaaca cggacatcg 
      actcggggt tcgaaaata tggaggtcg acgagaccg 
      acgtccaga agaaagacg cccctcgtc aacgaacat 
      catcctcag cgaccgaaa tcaccgaag aacaactcc 
      tagcaacgg tcctccgca tcctccatt ccaccctcg 
      aacaaccat ccgaaagtc gcatttatc tccctcaaa 
      ccgccaccg acgtcaaaa cccaccctc aaacctcaa 
      tccgcgaca tcatctcca acgcactga tggaaatca 
      tcgtccaaa tctcacaag gcaataacc ccttcaaag 
      acctgatcc cgagcaacg acacagccg acaccagca 
      gcataaact cgctaagtc cctccccca gccctagcc 
      acacctgcc ccataccca ccctgccaa tcaaacgag 
      tggaagtgg cggaaacgc ccctaaacc tagcactga 
      attacctaa tggattgct acaatcgtg tgagcgtcg 
      aggaacgaa accacccga agcgatcga gtattcccc 
      cgttaagtg acgttttaa cggaagttc gggcacaac 
      tggtgcact acacaacgt tgttactcc aaggtttta 
      tcatagcca atgacgagt tgtacaaat tcgggcgtt 
      cgccccttt tgagcccca ttttgcagc agacgctcc 
      cgcgggggt tcctcaccg agctggtga cccgaaccc 
      acacgaaca cgtcgttgt tctcgtcgg ttttctcgc 
      cactgcttc agggacttt accgaatga tgcagaacg 
      cgagtatcg tgggcgagg agcaccgaa tgccgatcc 
      agacattgt tcgagttgg ccgatattc ggtgcggca 
      gccgcccca tctgtacgc taatcacga aacaagtgc 
      aatttatgc tggagccgt gtacgcctg cgcaaggcc 
      gcgaagttg tccgaacgt cgtttctcg ggccgtttc 
      tgtcggtgt cggtgttcg ccgaagacg agccgaaag 
      tggccgaga agttcttct cggggaatt cgtcgagtg 
      ataagagtt tgaatcata cgctgcagc tggagctgg 
      agaatacgc ggaatttgc aacatagaa tttttcatt 
      atttgttgg cgaatccgg agagtcgtg atgggtgtt 
      tgaattatt tggcgaagg ttttgaagt ataatgaga 
      agagggcgc agctgcaga tggaggaga ggtcgctgg 
      ctggggacg ggtttatgt tgaatttgt taagtgttt 
      tgcaaatgt tgtcgatga agattaagt tggataaga 
      tggattttt tgtatccgt ttcattccg aaagtttga 
      tttgtatta agaatgata cgaggttga agtatacgt 
      cgcaggatg tcgccacgt ggctggaga gtttggaaa 
      aaacccacc aattccaaa cgcccaact tttcgacaa 
      tttgttggt tcttgacac tccactgcc atcacctcg 
      ccctccttc ccgcactcc aaaaatacc aacgtcgca 
      ttcgcgcca ttcgtgatc tacaaaaac tcctcgacg 
      aaacggtgg ctgcggaag cacagtggc gtaacacac 
      cgttcgcca atcgcaata aacagttta tcaaacggt 
      ggaaacaac aattgaaaa aattgaaca aatctaaag 
      cgtgcgctg atgccggac tactagata agaatctaa 
      tgcgacgcg ctttgattt tttacacct cagtcgctc 
      agtatttac tgagtctga tgacaaata tggcgccgg 
      ggagtcccg tgccgagtc taccactgc cgccgaacg 
      tacccgaag cattctcgg cgcttcccg aatggtacg 
      tcgaagata tagccgagt ttcttctat ttgccttac 
      catatttcc cgaccgtca tcacggaga acatggaag 
      attccctaa tcacgtggt tgctagtga caatttgtt 
      cctccaaca tggtcaaaa atccctact tggtggcca 
      aactggtcg aagtcgtct tcataataa tcccaacgt 
      ttcaaccgc gttgcgaaa tgttgtacg accgtttca 
      tgtcgcacg aaatttcac gaagtgtgc tacccagtg 
      ccctgatga agttctaca ccgatgtcg aaacgaccg 
      gctccagtt cggaatttt acgacaagt tttcaatcc 
      gttaccata tcagtctca taattaaag gcatgtgga 
      actcggcaa tacatcgcc aaacgctcg tcaacgaat 
      caaaatcag gaaaacagt tcgtcaaat tcgtcaata 
      tgctaatga acgacacca ctttcctcc tcgacgaga 
      gtctcgaaa gtttgaaac gcatccacg aagtgcaag 
      agttgataa gcgatgagg agaaatggt cgaaaatga 
      attcggaac agcagcaaa gtcggatga gacaattga 
      cagccgatg agagacagt gtcgctcct acttgactc 
      tagctcgtg aaactgtcg atatgtttc actacttaa 
      cggttgata ttaaggagc cgtttttgc gaccggaac 
      ttgttgatc gtttagcgt cgatgctta attttaatt 
      tgcaacaat tgtgtggcc cgaaatgta aaaatttga 
      aagtgagaa atccggata agtatggct gggagccgc 
      gacggttgt tgagtcaat tggttgata tttatctgc 
      atttggact gcgaggagt tcgccgccg ctttggccg 
      gcgatgaga gatctttcc ggaaagatt tgtttgacg 
      acgcagcgg cgcgattag agcgcctgt caatcaaaa 
      ctccggttg agatcgaac ggtttaagg ccttggccg 
      ataaggcgt atcacgtgt atcaaaata accagaaaa 
      gtgatgact ggatgagcg acgcccctg acgaattca 
      aggatccct taatggaca ctttgatga ctgatcctg 
      tgttgttgc ccagtgggc aggtgatgg acaggtccg 
      tcattatga ggcatttgt tgaatagca gtacggacc 
      cgtttaatc ggcaaccat tgactgaag atatgctgc 
      agccagtga atgagttga aggagcgca ttaggatat 
      ggaaatcag aaaagacac gttcggcaa attaatttt 
      attattttt tggtataaa tataatttt tggataaaa 
      ctgagttta tttcgaccg gaaggaagg aaggctgag 
      aatctcgtt tcgtttgca tttgttcct caaattcat 
      actgccaaa ttgttgctt ttttagttc agtaattaa 
      tgccaactt attgtttgt cttgacttt cccccagtc 
      cggtggatt atttttgta cataattat acaaaacgc 
      aaagtgaga tgtacattt ttttcatct aatcgaatt 
      aactgttag tgcaaacaa atttgattg tttcccgat 
      attttttct agataattt taggttaaa gcttgtaga 
      caccgaaat aatcaaatt tatttgtaa tgaaaacaa 
      agcaaaaca atttttata tgaaaacgt aggttagtt 
      tgtatgtaa tgggtcaat aaaatttat acaaaaatc 
      agatatgtg ttttcgtaa aagccctat catgcaata 
      ctgtctcgg taactagag gtttgtccg acaaaacag 
      ctcgtaagt aaaaaacgg ttcaggact aaaacttcc 
      gcaggaaca aacctgtaa atcccacta gaccaacaa 
      ttttaaatg ctatgattg tgctattat cactaccga 
      aaattgtgc taaaggctg tccctgacg cgctttgaa 
      taattgatt aagtcataa gccccgaac aagccgcca 
      ggtggccaa cccacacac tacgaataa attatttga 
      tatttttac gcagacttt tccgttatt gtcaattga 
      agtagtctg agcagtgac tggaagaga tgcgattgc 
      agcgccccc aaacgtcaa atggcgcct actaccact 
      accaagcgt ttcgtttaa tattagtct agacacaca 
      aaataaaaa caaacggaa attataatg atttactat 
      aaaatctct tacacaacg tcatacaaa actcatcaa 
      cattaacaa taaattaat cagctataa aaatacatg 
      atttataat tggccaatc agggactaa accccgcct 
      tgaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY