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Gene information
General information
TC033018
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030343,TC030345
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1411930..1426384
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033018
    iBeetle screen iB_08906

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033018 RA
      MVLRKTMLI KNSSLYEEI LELQFYFWK YGLFGETTG 
      SKLRKSMKT CVSVLKFIV INGSMSVTL HSISPLLVK 
      SLVLPQSCW IPGNNPVAK NVIHIFLVI MYVECLNYL 
      VVFDGLYLL TATNLKAQF ILLQKAADS IKLTRGEEE 
      LSWMKLKKF SQYHAFILR GDVPIIILI KSKTLYKLL 
      VLLLHKMSF NESEDNLKL CLACYNLSG LGPSSKLFL 
      RLLSYILYI LLWVFLIMV IINIAFKHD NIWDIAEIF 
      TSVSITITM VIRKTIMLK HSSSFEELI EMHSQFWDY 
      SLFGKTTES KIRKSTGLY KFILKCYVV CGVISVSLR 
      SLAPIFAKN LVLPQDCWI PGNSTIARF IIYAFETVL 
      YAEGVSYYT FFDGLLLMI TANLKAQFI LLQKAIQSI 
      NFETDSSET AWVKLVKCC EHHKFLLSV LKKLNSLYS 
      IFYLCTYIL VITGVCVSL FIVFDKSST FAQIVEGAI 
      TVVILNSMI FMICICSSE TEIQAEKLI TQIYDINWY 
      DSPNLKIRK FILFWLMQA QVSVQIKGA GVLVLNRPL 
      MLQVQRISY SVSTLLTGL NE
    CDS sequence
    • >TC033018 RA
      atggttctc cgaaaaaca atgctgata aaaaacagt 
      tcgttatat gaggaaatt ctagaactg cagttttat 
      ttctggaag tatggctta tttggggaa accactggc 
      tctaaacta cgaaaaagt atgaaaact tgcgtttct 
      gtcctgaaa tttattgtt atcaacggc tccatgtcc 
      gtaactctt cactcgatt tccccgctt cttgtcaaa 
      agtttggtg ctacctcag tcgtgttgg attcctgga 
      aataatcct gttgctaaa aatgttata cacattttc 
      ctagtgata atgtacgtg gagtgtttg aattatctg 
      gttgttttt gacggcctg tatttacta acggccaca 
      aatctcaaa gcacaattt atccttttg caaaaagct 
      gctgattcc attaaactg accagaggt gaggaagaa 
      ctctcctgg atgaaactg aaaaagttc agccagtat 
      cacgcattt attttaagg ggcgatgtt cctattatc 
      attttaatc aagagtaaa acgttgtac aagttgctt 
      gttttactc ttgcacaaa atgagtttt aatgaaagt 
      gaagacaat ttgaaactg tgtcttgct tgctacaac 
      ttgagtggt ttagggcct tcaagtaaa ttatttcta 
      agacttttg tcctacata ctttacatt cttttgtgg 
      gttttcctg attatggta attattaat attgccttc 
      aaacatgac aacatctgg gacattgct gaaatcttc 
      acttcagtc tccattact ataacaatg gtcattaga 
      aagactatt atgcttaaa cacagttct tcgtttgag 
      gaacttatc gaaatgcat tctcaattt tgggattat 
      agtcttttt gggaaaacc actgagtcc aagattcga 
      aaaagtaca ggattatac aaattcatt ttaaagtgt 
      tatgtagtg tgtggagtt atttccgta tcacttcgt 
      tctttggca ccgatattt gcaaagaat cttgtatta 
      ccacaggac tgttggatt cctggaaat agtactata 
      gcaagattt ataatttat gctttcgaa accgtactt 
      tatgcagaa ggcgtttct tattatacg tttttcgat 
      ggtttgctt ttaatgatc accgcaaat cttaaagca 
      caattcatt ttgttgcaa aaagcaatt caatccata 
      aattttgaa actgactct agtgaaaca gcttgggtt 
      aaactagta aaatgttgt gaacatcac aaattcttg 
      ttaagtgta cttaagaag ttaaactcg ttgtactcc 
      attttttat ctgtgtact tacattctt gtaataact 
      ggagtttgt gtatcgttg tttattgtt tttgacaag 
      tcctcaact tttgcccaa attgtggaa ggcgcaatt 
      actgtagtt atattaaac agtatgatt ttcatgatc 
      tgcatttgt agtagtgaa acagagatt caagctgaa 
      aaattgatc acacaaatt tacgacatc aattggtac 
      gactcacca aatttaaaa attcgcaag tttatccta 
      ttttggctc atgcaagca caggtttcc gttcaaata 
      aaaggagca ggagtgttg gtactaaat agacctttg 
      atgcttcag gttcaacgt atttcatat tcagtatct 
      acattatta acaggacta aatgaataa
    mRNA sequence
    • >TC033018 RA
      attaatcaa caaataaac gccagattg taaaggctc 
      cggttgata tccccaacc aacaattaa aattaaagt 
      cagaaatta acttacctc aaaaaatat aaaaaataa 
      acaaattta aagaactaa aagttaaat atactctag 
      ccagtacca ttaaaatac aaattcaaa aactaagga 
      acaacaaag aagaagatg gttctccga aaaacaatg 
      ctgataaaa aacagttcg ttatatgag gaaattcta 
      gaactgcag ttttatttc tggaagtat ggcttattt 
      ggggaaacc actggctct aaactacga aaaagtatg 
      aaaacttgc gtttctgtc ctgaaattt attgttatc 
      aacggctcc atgtccgta actcttcac tcgatttcc 
      ccgcttctt gtcaaaagt ttggtgcta cctcagtcg 
      tgttggatt cctggaaat aatcctgtt gctaaaaat 
      gttatacac attttccta gtgataatg tacgtggag 
      tgtttgaat tatctggtt gtttttgac ggcctgtat 
      ttactaacg gccacaaat ctcaaagca caatttatc 
      cttttgcaa aaagctgct gattccatt aaactgacc 
      agaggtgag gaagaactc tcctggatg aaactgaaa 
      aagttcagc cagtatcac gcatttatt ttaaggggc 
      gatgttcct attatcatt ttaatcaag agtaaaacg 
      ttgtacaag ttgcttgtt ttactcttg cacaaaatg 
      agttttaat gaaagtgaa gacaatttg aaactgtgt 
      cttgcttgc tacaacttg agtggttta gggccttca 
      agtaaatta tttctaaga cttttgtcc tacatactt 
      tacattctt ttgtgggtt ttcctgatt atggtaatt 
      attaatatt gccttcaaa catgacaac atctgggac 
      attgctgaa atcttcact tcagtctcc attactata 
      acaatggtc attagaaag actattatg cttaaacac 
      agttcttcg tttgaggaa cttatcgaa atgcattct 
      caattttgg gattatagt ctttttggg aaaaccact 
      gagtccaag attcgaaaa agtacagga ttatacaaa 
      ttcatttta aagtgttat gtagtgtgt ggagttatt 
      tccgtatca cttcgttct ttggcaccg atatttgca 
      aagaatctt gtattacca caggactgt tggattcct 
      ggaaatagt actatagca agatttata atttatgct 
      ttcgaaacc gtactttat gcagaaggc gtttcttat 
      tatacgttt ttcgatggt ttgctttta atgatcacc 
      gcaaatctt aaagcacaa ttcattttg ttgcaaaaa 
      gcaattcaa tccataaat tttgaaact gactctagt 
      gaaacagct tgggttaaa ctagtaaaa tgttgtgaa 
      catcacaaa ttcttgtta agtgtactt aagaagtta 
      aactcgttg tactccatt ttttatctg tgtacttac 
      attcttgta ataactgga gtttgtgta tcgttgttt 
      attgttttt gacaagtcc tcaactttt gcccaaatt 
      gtggaaggc gcaattact gtagttata ttaaacagt 
      atgattttc atgatctgc atttgtagt agtgaaaca 
      gagattcaa gctgaaaaa ttgatcaca caaatttac 
      gacatcaat tggtacgac tcaccaaat ttaaaaatt 
      cgcaagttt atcctattt tggctcatg caagcacag 
      gtttccgtt caaataaaa ggagcagga gtgttggta 
      ctaaataga cctttgatg cttcaggtt caacgtatt 
      tcatattca gtatctaca ttattaaca ggactaaat 
      gaataagta attaaattt ccatgccac aactgttgc 
      ttaatgctt atactcgta acccaatgt ttgaaataa 
      gttgttttg gtatggaag cgcaatata tatattata 
      gagca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY