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Gene information
General information
TC033227
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030119,TC013139 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13417884..13421341
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033227
    iBeetle screen iB_08903

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033227 RA
      MKVIIALCA FFAASCAQQ PATSEPIPI VKYDNEGVN 
ADGSYQWSY ETGNGINAQ EQGQIKNAG SENEAAEVQ 
GSASWTAPD GTAISLNYI ANEDGFQPQ GDHLPTPPP 
IPPAIQRAL EWIAAHPEP EEAAAPAAS NLKPVTVLE 
PVHKMKAPQ SGLLLIQPQ NNFHKSLRF LFVIAQVFG 
YFPVQGVSE EKVTGISFT WFSLRVLSS LLTAFLGIV 
VIFAQIRFM KLITGYEQA QMNAIVFYG SGTLSSFLF 
IKLARDWHE IMTKWNQLD KALISHGWP KGLDKTLKR 
IAIIFLALE AAEHFTIQA NKLIVAVRC RGSFSKGFR 
YFAVDMSFA VVFDFVDYA HWLGMIFQL MNTRMAFAW 
TFLDLFIIL VSCALSERF RQINDRIQH LTDIKSKHF 
KEWIAINED YNRLCLFCD YLDDKISWL ILISFFNNF 
YFIIFQVHL SLNLVLAPP LEIIYYLIS SSLLILRMV 
SVCIYANRV HEESKSPMD ILSVAPLEI YNQENIRLI 
VTAKYQTTG LTARKMFLL TKNLIFAIA GAVLAYELI 
VIQLNKNQP RKQGPSIYS LC
    CDS sequence
    • >TC033227 RA
      atgaaagtg ataattgct ctctgcgcc ttttttgcg 
gcctcctgt gcccaacaa cccgccacc agcgagccc 
atccccata gtgaaatac gacaacgaa ggagtcaat 
gctgatggg tcttaccag tggagctac gaaaccggc 
aacggaatc aatgcccaa gaacaagga caaataaaa 
aacgctggc tctgaaaac gaagctgct gaagtgcaa 
ggatcggcc tcctggacc gcccccgac ggcactgcc 
atttccctc aactacatc gccaacgag gacggcttc 
cagcctcaa ggtgaccac ttgccgacc ccacccccg 
attccccca gcgatccaa cgagctttg gagtggatt 
gctgcccat ccagagccc gaagaagcc gccgctcca 
gctgcgagc aacttgaaa ccagtaact gtacttgaa 
ccagtccac aaaatgaaa gctccacaa tctgggctg 
ctcttgata cagccccaa aacaatttt cacaaatct 
ttgcgattc ctgttcgta atcgcacaa gtatttggg 
tattttccg gtacagggt gtttccgaa gaaaaagtg 
actgggatc agtttcact tggttttcc ctgcgtgtg 
ttatcgtca ctattaaca gcgtttttg ggtattgtg 
gtgattttt gcccaaatt agattcatg aaacttatc 
acaggctat gagcaagca cagatgaat gcgatcgtt 
ttttacggt tctgggact ttgtccagt tttctcttt 
ataaaacta gccagagat tggcacgaa attatgacc 
aagtggaac caactagac aaagctttg atatcacac 
ggttggcct aaaggccta gacaaaact ttgaaacgt 
attgccatc attttttta gcacttgaa gccgctgaa 
cattttacg attcaggct aataaattg atagtggca 
gtgcgttgt cgtggatca ttttctaaa ggttttcgc 
tattttgcc gtcgatatg tcgtttgca gtcgttttt 
gattttgtc gattatgcc cactggctt ggcatgatt 
tttcagtta atgaacacc agaatggca tttgcgtgg 
acgttcctc gacttgttc attattttg gtcagttgt 
gctttaagt gagaggttt cggcaaatc aacgaccga 
attcaacac ttgacagat attaaatca aaacacttc 
aaagaatgg attgcaatt aacgaagat tataatcgc 
ttgtgccta ttttgcgac tatttagac gataaaatt 
tcgtggctg attttaatc tcgttcttc aacaatttt 
tatttcatt atttttcaa gtccatctt agtctaaat 
cttgttctg gcgccgcct ttggaaatt atttattat 
ttgatttcc tcaagttta ctcattctc agaatggtg 
tctgtttgt atttatgca aatcgagtc catgaagaa 
agtaaatca ccaatggat attctttca gttgcgcct 
ttagaaatc tacaaccag gagaacatc agacttatt 
gttactgca aagtatcaa actaccgga cttacggcg 
cgaaaaatg tttcttctt acaaaaaat cttattttt 
gccattgct ggagcggtg ctcgcttat gaattaata 
gtgatacaa cttaataaa aatcagcca aggaagcag 
ggaccatca atttactca ctgtgttaa
    mRNA sequence
    • >TC033227 RA
      tccgacgct ccttttcaa caaaaccaa aatgaaagt 
gataattgc tctctgcgc cttttttgc ggcctcctg 
tgcccaaca acccgccac cagcgagcc catccccat 
agtgaaata cgacaacga aggagtcaa tgctgatgg 
gtcttacca gtggagcta cgaaaccgg caacggaat 
caatgccca agaacaagg acaaataaa aaacgctgg 
ctctgaaaa cgaagctgc tgaagtgca aggatcggc 
ctcctggac cgcccccga cggcactgc catttccct 
caactacat cgccaacga ggacggctt ccagcctca 
aggtgacca cttgccgac cccaccccc gattccccc 
agcgatcca acgagcttt ggagtggat tgctgccca 
tccagagcc cgaagaagc cgccgctcc agctgcgag 
caacttgaa accagtaac tgtacttga accagtcca 
caaaatgaa agctccaca atctgggct gctcttgat 
acagcccca aaacaattt tcacaaatc tttgcgatt 
cctgttcgt aatcgcaca agtatttgg gtattttcc 
ggtacaggg tgtttccga agaaaaagt gactgggat 
cagtttcac ttggttttc cctgcgtgt gttatcgtc 
actattaac agcgttttt gggtattgt ggtgatttt 
tgcccaaat tagattcat gaaacttat cacaggcta 
tgagcaagc acagatgaa tgcgatcgt tttttacgg 
ttctgggac tttgtccag ttttctctt tataaaact 
agccagaga ttggcacga aattatgac caagtggaa 
ccaactaga caaagcttt gatatcaca cggttggcc 
taaaggcct agacaaaac tttgaaacg tattgccat 
cattttttt agcacttga agccgctga acattttac 
gattcaggc taataaatt gatagtggc agtgcgttg 
tcgtggatc attttctaa aggttttcg ctattttgc 
cgtcgatat gtcgtttgc agtcgtttt tgattttgt 
cgattatgc ccactggct tggcatgat ttttcagtt 
aatgaacac cagaatggc atttgcgtg gacgttcct 
cgacttgtt cattatttt ggtcagttg tgctttaag 
tgagaggtt tcggcaaat caacgaccg aattcaaca 
cttgacaga tattaaatc aaaacactt caaagaatg 
gattgcaat taacgaaga ttataatcg cttgtgcct 
attttgcga ctatttaga cgataaaat ttcgtggct 
gattttaat ctcgttctt caacaattt ttatttcat 
tatttttca agtccatct tagtctaaa tcttgttct 
ggcgccgcc tttggaaat tatttatta tttgatttc 
ctcaagttt actcattct cagaatggt gtctgtttg 
tatttatgc aaatcgagt ccatgaaga aagtaaatc 
accaatgga tattctttc agttgcgcc tttagaaat 
ctacaacca ggagaacat cagacttat tgttactgc 
aaagtatca aactaccgg acttacggc gcgaaaaat 
gtttcttct tacaaaaaa tcttatttt tgccattgc 
tggagcggt gctcgctta tgaattaat agtgataca 
acttaataa aaatcagcc aaggaagca gggaccatc 
aatttactc actgtgtta aaatattaa aaacaaaaa 
aaatttaat aaacgtgta gtaatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY