Status
Please wait ...
Gene information
General information
TC033227
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030119,TC013139
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13417884..13421341
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033227
    iBeetle screen iB_08903

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033227 RA
      MKVIIALCA FFAASCAQQ PATSEPIPI VKYDNEGVN 
      ADGSYQWSY ETGNGINAQ EQGQIKNAG SENEAAEVQ 
      GSASWTAPD GTAISLNYI ANEDGFQPQ GDHLPTPPP 
      IPPAIQRAL EWIAAHPEP EEAAAPAAS NLKPVTVLE 
      PVHKMKAPQ SGLLLIQPQ NNFHKSLRF LFVIAQVFG 
      YFPVQGVSE EKVTGISFT WFSLRVLSS LLTAFLGIV 
      VIFAQIRFM KLITGYEQA QMNAIVFYG SGTLSSFLF 
      IKLARDWHE IMTKWNQLD KALISHGWP KGLDKTLKR 
      IAIIFLALE AAEHFTIQA NKLIVAVRC RGSFSKGFR 
      YFAVDMSFA VVFDFVDYA HWLGMIFQL MNTRMAFAW 
      TFLDLFIIL VSCALSERF RQINDRIQH LTDIKSKHF 
      KEWIAINED YNRLCLFCD YLDDKISWL ILISFFNNF 
      YFIIFQVHL SLNLVLAPP LEIIYYLIS SSLLILRMV 
      SVCIYANRV HEESKSPMD ILSVAPLEI YNQENIRLI 
      VTAKYQTTG LTARKMFLL TKNLIFAIA GAVLAYELI 
      VIQLNKNQP RKQGPSIYS LC
    CDS sequence
    • >TC033227 RA
      atgaaagtg ataattgct ctctgcgcc ttttttgcg 
      gcctcctgt gcccaacaa cccgccacc agcgagccc 
      atccccata gtgaaatac gacaacgaa ggagtcaat 
      gctgatggg tcttaccag tggagctac gaaaccggc 
      aacggaatc aatgcccaa gaacaagga caaataaaa 
      aacgctggc tctgaaaac gaagctgct gaagtgcaa 
      ggatcggcc tcctggacc gcccccgac ggcactgcc 
      atttccctc aactacatc gccaacgag gacggcttc 
      cagcctcaa ggtgaccac ttgccgacc ccacccccg 
      attccccca gcgatccaa cgagctttg gagtggatt 
      gctgcccat ccagagccc gaagaagcc gccgctcca 
      gctgcgagc aacttgaaa ccagtaact gtacttgaa 
      ccagtccac aaaatgaaa gctccacaa tctgggctg 
      ctcttgata cagccccaa aacaatttt cacaaatct 
      ttgcgattc ctgttcgta atcgcacaa gtatttggg 
      tattttccg gtacagggt gtttccgaa gaaaaagtg 
      actgggatc agtttcact tggttttcc ctgcgtgtg 
      ttatcgtca ctattaaca gcgtttttg ggtattgtg 
      gtgattttt gcccaaatt agattcatg aaacttatc 
      acaggctat gagcaagca cagatgaat gcgatcgtt 
      ttttacggt tctgggact ttgtccagt tttctcttt 
      ataaaacta gccagagat tggcacgaa attatgacc 
      aagtggaac caactagac aaagctttg atatcacac 
      ggttggcct aaaggccta gacaaaact ttgaaacgt 
      attgccatc attttttta gcacttgaa gccgctgaa 
      cattttacg attcaggct aataaattg atagtggca 
      gtgcgttgt cgtggatca ttttctaaa ggttttcgc 
      tattttgcc gtcgatatg tcgtttgca gtcgttttt 
      gattttgtc gattatgcc cactggctt ggcatgatt 
      tttcagtta atgaacacc agaatggca tttgcgtgg 
      acgttcctc gacttgttc attattttg gtcagttgt 
      gctttaagt gagaggttt cggcaaatc aacgaccga 
      attcaacac ttgacagat attaaatca aaacacttc 
      aaagaatgg attgcaatt aacgaagat tataatcgc 
      ttgtgccta ttttgcgac tatttagac gataaaatt 
      tcgtggctg attttaatc tcgttcttc aacaatttt 
      tatttcatt atttttcaa gtccatctt agtctaaat 
      cttgttctg gcgccgcct ttggaaatt atttattat 
      ttgatttcc tcaagttta ctcattctc agaatggtg 
      tctgtttgt atttatgca aatcgagtc catgaagaa 
      agtaaatca ccaatggat attctttca gttgcgcct 
      ttagaaatc tacaaccag gagaacatc agacttatt 
      gttactgca aagtatcaa actaccgga cttacggcg 
      cgaaaaatg tttcttctt acaaaaaat cttattttt 
      gccattgct ggagcggtg ctcgcttat gaattaata 
      gtgatacaa cttaataaa aatcagcca aggaagcag 
      ggaccatca atttactca ctgtgttaa
    mRNA sequence
    • >TC033227 RA
      tccgacgct ccttttcaa caaaaccaa aatgaaagt 
      gataattgc tctctgcgc cttttttgc ggcctcctg 
      tgcccaaca acccgccac cagcgagcc catccccat 
      agtgaaata cgacaacga aggagtcaa tgctgatgg 
      gtcttacca gtggagcta cgaaaccgg caacggaat 
      caatgccca agaacaagg acaaataaa aaacgctgg 
      ctctgaaaa cgaagctgc tgaagtgca aggatcggc 
      ctcctggac cgcccccga cggcactgc catttccct 
      caactacat cgccaacga ggacggctt ccagcctca 
      aggtgacca cttgccgac cccaccccc gattccccc 
      agcgatcca acgagcttt ggagtggat tgctgccca 
      tccagagcc cgaagaagc cgccgctcc agctgcgag 
      caacttgaa accagtaac tgtacttga accagtcca 
      caaaatgaa agctccaca atctgggct gctcttgat 
      acagcccca aaacaattt tcacaaatc tttgcgatt 
      cctgttcgt aatcgcaca agtatttgg gtattttcc 
      ggtacaggg tgtttccga agaaaaagt gactgggat 
      cagtttcac ttggttttc cctgcgtgt gttatcgtc 
      actattaac agcgttttt gggtattgt ggtgatttt 
      tgcccaaat tagattcat gaaacttat cacaggcta 
      tgagcaagc acagatgaa tgcgatcgt tttttacgg 
      ttctgggac tttgtccag ttttctctt tataaaact 
      agccagaga ttggcacga aattatgac caagtggaa 
      ccaactaga caaagcttt gatatcaca cggttggcc 
      taaaggcct agacaaaac tttgaaacg tattgccat 
      cattttttt agcacttga agccgctga acattttac 
      gattcaggc taataaatt gatagtggc agtgcgttg 
      tcgtggatc attttctaa aggttttcg ctattttgc 
      cgtcgatat gtcgtttgc agtcgtttt tgattttgt 
      cgattatgc ccactggct tggcatgat ttttcagtt 
      aatgaacac cagaatggc atttgcgtg gacgttcct 
      cgacttgtt cattatttt ggtcagttg tgctttaag 
      tgagaggtt tcggcaaat caacgaccg aattcaaca 
      cttgacaga tattaaatc aaaacactt caaagaatg 
      gattgcaat taacgaaga ttataatcg cttgtgcct 
      attttgcga ctatttaga cgataaaat ttcgtggct 
      gattttaat ctcgttctt caacaattt ttatttcat 
      tatttttca agtccatct tagtctaaa tcttgttct 
      ggcgccgcc tttggaaat tatttatta tttgatttc 
      ctcaagttt actcattct cagaatggt gtctgtttg 
      tatttatgc aaatcgagt ccatgaaga aagtaaatc 
      accaatgga tattctttc agttgcgcc tttagaaat 
      ctacaacca ggagaacat cagacttat tgttactgc 
      aaagtatca aactaccgg acttacggc gcgaaaaat 
      gtttcttct tacaaaaaa tcttatttt tgccattgc 
      tggagcggt gctcgctta tgaattaat agtgataca 
      acttaataa aaatcagcc aaggaagca gggaccatc 
      aatttactc actgtgtta aaatattaa aaacaaaaa 
      aaatttaat aaacgtgta gtaatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY