GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08903 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033227 RA
MKVIIALCA FFAASCAQQ PATSEPIPI VKYDNEGVN ADGSYQWSY ETGNGINAQ EQGQIKNAG SENEAAEVQ GSASWTAPD GTAISLNYI ANEDGFQPQ GDHLPTPPP IPPAIQRAL EWIAAHPEP EEAAAPAAS NLKPVTVLE PVHKMKAPQ SGLLLIQPQ NNFHKSLRF LFVIAQVFG YFPVQGVSE EKVTGISFT WFSLRVLSS LLTAFLGIV VIFAQIRFM KLITGYEQA QMNAIVFYG SGTLSSFLF IKLARDWHE IMTKWNQLD KALISHGWP KGLDKTLKR IAIIFLALE AAEHFTIQA NKLIVAVRC RGSFSKGFR YFAVDMSFA VVFDFVDYA HWLGMIFQL MNTRMAFAW TFLDLFIIL VSCALSERF RQINDRIQH LTDIKSKHF KEWIAINED YNRLCLFCD YLDDKISWL ILISFFNNF YFIIFQVHL SLNLVLAPP LEIIYYLIS SSLLILRMV SVCIYANRV HEESKSPMD ILSVAPLEI YNQENIRLI VTAKYQTTG LTARKMFLL TKNLIFAIA GAVLAYELI VIQLNKNQP RKQGPSIYS LC
- >TC033227 RA
atgaaagtg ataattgct ctctgcgcc ttttttgcg gcctcctgt gcccaacaa cccgccacc agcgagccc atccccata gtgaaatac gacaacgaa ggagtcaat gctgatggg tcttaccag tggagctac gaaaccggc aacggaatc aatgcccaa gaacaagga caaataaaa aacgctggc tctgaaaac gaagctgct gaagtgcaa ggatcggcc tcctggacc gcccccgac ggcactgcc atttccctc aactacatc gccaacgag gacggcttc cagcctcaa ggtgaccac ttgccgacc ccacccccg attccccca gcgatccaa cgagctttg gagtggatt gctgcccat ccagagccc gaagaagcc gccgctcca gctgcgagc aacttgaaa ccagtaact gtacttgaa ccagtccac aaaatgaaa gctccacaa tctgggctg ctcttgata cagccccaa aacaatttt cacaaatct ttgcgattc ctgttcgta atcgcacaa gtatttggg tattttccg gtacagggt gtttccgaa gaaaaagtg actgggatc agtttcact tggttttcc ctgcgtgtg ttatcgtca ctattaaca gcgtttttg ggtattgtg gtgattttt gcccaaatt agattcatg aaacttatc acaggctat gagcaagca cagatgaat gcgatcgtt ttttacggt tctgggact ttgtccagt tttctcttt ataaaacta gccagagat tggcacgaa attatgacc aagtggaac caactagac aaagctttg atatcacac ggttggcct aaaggccta gacaaaact ttgaaacgt attgccatc attttttta gcacttgaa gccgctgaa cattttacg attcaggct aataaattg atagtggca gtgcgttgt cgtggatca ttttctaaa ggttttcgc tattttgcc gtcgatatg tcgtttgca gtcgttttt gattttgtc gattatgcc cactggctt ggcatgatt tttcagtta atgaacacc agaatggca tttgcgtgg acgttcctc gacttgttc attattttg gtcagttgt gctttaagt gagaggttt cggcaaatc aacgaccga attcaacac ttgacagat attaaatca aaacacttc aaagaatgg attgcaatt aacgaagat tataatcgc ttgtgccta ttttgcgac tatttagac gataaaatt tcgtggctg attttaatc tcgttcttc aacaatttt tatttcatt atttttcaa gtccatctt agtctaaat cttgttctg gcgccgcct ttggaaatt atttattat ttgatttcc tcaagttta ctcattctc agaatggtg tctgtttgt atttatgca aatcgagtc catgaagaa agtaaatca ccaatggat attctttca gttgcgcct ttagaaatc tacaaccag gagaacatc agacttatt gttactgca aagtatcaa actaccgga cttacggcg cgaaaaatg tttcttctt acaaaaaat cttattttt gccattgct ggagcggtg ctcgcttat gaattaata gtgatacaa cttaataaa aatcagcca aggaagcag ggaccatca atttactca ctgtgttaa
- >TC033227 RA
tccgacgct ccttttcaa caaaaccaa aatgaaagt gataattgc tctctgcgc cttttttgc ggcctcctg tgcccaaca acccgccac cagcgagcc catccccat agtgaaata cgacaacga aggagtcaa tgctgatgg gtcttacca gtggagcta cgaaaccgg caacggaat caatgccca agaacaagg acaaataaa aaacgctgg ctctgaaaa cgaagctgc tgaagtgca aggatcggc ctcctggac cgcccccga cggcactgc catttccct caactacat cgccaacga ggacggctt ccagcctca aggtgacca cttgccgac cccaccccc gattccccc agcgatcca acgagcttt ggagtggat tgctgccca tccagagcc cgaagaagc cgccgctcc agctgcgag caacttgaa accagtaac tgtacttga accagtcca caaaatgaa agctccaca atctgggct gctcttgat acagcccca aaacaattt tcacaaatc tttgcgatt cctgttcgt aatcgcaca agtatttgg gtattttcc ggtacaggg tgtttccga agaaaaagt gactgggat cagtttcac ttggttttc cctgcgtgt gttatcgtc actattaac agcgttttt gggtattgt ggtgatttt tgcccaaat tagattcat gaaacttat cacaggcta tgagcaagc acagatgaa tgcgatcgt tttttacgg ttctgggac tttgtccag ttttctctt tataaaact agccagaga ttggcacga aattatgac caagtggaa ccaactaga caaagcttt gatatcaca cggttggcc taaaggcct agacaaaac tttgaaacg tattgccat cattttttt agcacttga agccgctga acattttac gattcaggc taataaatt gatagtggc agtgcgttg tcgtggatc attttctaa aggttttcg ctattttgc cgtcgatat gtcgtttgc agtcgtttt tgattttgt cgattatgc ccactggct tggcatgat ttttcagtt aatgaacac cagaatggc atttgcgtg gacgttcct cgacttgtt cattatttt ggtcagttg tgctttaag tgagaggtt tcggcaaat caacgaccg aattcaaca cttgacaga tattaaatc aaaacactt caaagaatg gattgcaat taacgaaga ttataatcg cttgtgcct attttgcga ctatttaga cgataaaat ttcgtggct gattttaat ctcgttctt caacaattt ttatttcat tatttttca agtccatct tagtctaaa tcttgttct ggcgccgcc tttggaaat tatttatta tttgatttc ctcaagttt actcattct cagaatggt gtctgtttg tatttatgc aaatcgagt ccatgaaga aagtaaatc accaatgga tattctttc agttgcgcc tttagaaat ctacaacca ggagaacat cagacttat tgttactgc aaagtatca aactaccgg acttacggc gcgaaaaat gtttcttct tacaaaaaa tcttatttt tgccattgc tggagcggt gctcgctta tgaattaat agtgataca acttaataa aaatcagcc aaggaagca gggaccatc aatttactc actgtgtta aaatattaa aaacaaaaa aaatttaat aaacgtgta gtaatttt
