Status
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Gene information
General information
TC012955 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 79627..84826
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012955
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08900

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012955 RA
      MEKPTNSFC KNVRTYFRE YCNCTSIHG FRYFGEKRT 
      IFERIWWII VFLICLSAC IFSIYGVYK KWDGSPVIV 
      NFANQGTSI YQIPFPAVT ICPESKSVQ NLFSFTKIM 
      LKKLRRQNT TQAEDTKFE YMALVCKYH EKINYTNPN 
      TFPDEFLDV LNTVKPNLI KWNCMYLGE KYDCNQMFV 
      KILTDEGIC YTFNMLDRT DIFRDKVLQ YKDYQRSLY 
      KMSEWSVEH GYTENAQFS PYPRRALLA GASNGLTLN 
      LMTPKNYLD YACRQTSLQ GYKVILHTP MRIPRPSQQ 
      YFRVPLDQN VVGAVQPVM ISTSDRVQM YDPKRRGCY 
      FPSERHLKY FKIYTSLNC KLECLTNYT LSRCKCVNF 
      FMPRESGTN ICGTGKIAC MREAERFLQ AKDLYLKLS 
      KNKSLKKTS QCDCLPICT DTTYEVETS QSDWDWKMT 
      YSAHGTPWP NDTHLSSLT IFFKSDTFI TSERNELYG 
      PIDFLANFG GLLGLFTGF SVLSLMEIL YFLSVRIIC 
      NNRLYGFWA GPSI
    CDS sequence
    • >TC012955 RA
      atggaaaaa cctacaaac tccttttgt aaaaatgtt 
      cgaacttat tttcgcgaa tactgcaac tgtacaagt 
      attcatggt tttcgttac ttcggcgag aaacgaaca 
      atattcgaa aggatttgg tggattata gttttctta 
      atttgttta tctgcgtgc attttttcg atttacgga 
      gtttacaaa aaatgggat ggaagtcca gtaattgta 
      aattttgca aatcagggg acttcgatt tatcaaata 
      ccttttcct gctgtgaca atttgtcct gaatcgaaa 
      tcagtccag aatttgttt agtttcacg aaaattatg 
      cttaagaag cttcgacgc caaaatacg actcaagcc 
      gaagacaca aaatttgag tatatggca cttgtgtgc 
      aaataccac gagaaaatc aactacaca aacccaaat 
      actttcccc gatgaattt ttggatgtt ttaaataca 
      gtcaaaccg aatttaatc aaatggaat tgcatgtat 
      ctgggggag aaatacgac tgtaatcaa atgtttgtc 
      aagatttta actgatgaa ggaatttgc tacaccttc 
      aatatgttg gatagaaca gacatattc agggataag 
      gtattacaa tacaaggat taccaacgc tcgctctac 
      aaaatgagt gaatggtct gtagaacat ggctacaca 
      gaaaacgcc caattttcg ccatatcct cgtcgagct 
      ttgcttgct ggggcgtcg aacggtctt actctcaac 
      ctcatgaca cctaaaaat tacttggat tatgcctgc 
      cggcaaaca tctctccag ggatataaa gtcattttg 
      cacactccg atgcggatt cctcgacca agtcagcag 
      tattttcgt gttcctttg gatcaaaat gttgtgggg 
      gctgttcaa cctgtaatg atcagcact tcagatagg 
      gttcaaatg tatgaccct aaaagacgg ggttgttat 
      tttccctca gaaaggcat ttgaagtat tttaaaatt 
      tatacttct ctcaactgt aaactcgaa tgtttgacg 
      aactatacg ttaagtcgg tgcaaatgt gtgaatttt 
      ttcatgcca agagagagt ggaacaaac atttgtggc 
      actggcaaa atagcctgc atgagagaa gctgaaaga 
      tttttacag gccaaagat ttgtattta aaattatca 
      aaaaacaaa tcattgaag aaaacgagt caatgcgat 
      tgcctcccg atttgtact gacacaact tatgaagtt 
      gaaacttca caaagtgat tgggattgg aaaatgacc 
      tacagtgct catggcaca ccttggcca aacgacact 
      cacttatcc tctcttaca attttcttc aaatcggac 
      actttcatc acatctgaa cgaaatgaa ctctacggt 
      cctattgac tttttggca aattttggc ggtctcctt 
      ggccttttt acaggattc tctgtgctt tccttaatg 
      gaaattttg tattttcta tcagtgaga atcatttgc 
      aacaatcgg ttgtacggt ttttgggca ggccccagt 
      atttaa
    mRNA sequence
    • >TC012955 RA
      ttttaacgc aattattat tttaatcac caacttgca 
      acaatggaa aaacctaca aactccttt tgtaaaaat 
      gttcgaact tattttcgc gaatactgc aactgtaca 
      agtattcat ggttttcgt tacttcggc gagaaacga 
      acaatattc gaaaggatt tggtggatt atagttttc 
      ttaatttgt ttatctgcg tgcattttt tcgatttac 
      ggagtttac aaaaaatgg gatggaagt ccagtaatt 
      gtaaatttt gcaaatcag gggacttcg atttatcaa 
      atacctttt cctgctgtg acaatttgt cctgaatcg 
      aaatcagtc cagaatttg tttagtttc acgaaaatt 
      atgcttaag aagcttcga cgccaaaat acgactcaa 
      gccgaagac acaaaattt gagtatatg gcacttgtg 
      tgcaaatac cacgagaaa atcaactac acaaaccca 
      aatactttc cccgatgaa tttttggat gttttaaat 
      acagtcaaa ccgaattta atcaaatgg aattgcatg 
      tatctgggg gagaaatac gactgtaat caaatgttt 
      gtcaagatt ttaactgat gaaggaatt tgctacacc 
      ttcaatatg ttggataga acagacata ttcagggat 
      aaggtatta caatacaag gattaccaa cgctcgctc 
      tacaaaatg agtgaatgg tctgtagaa catggctac 
      acagaaaac gcccaattt tcgccatat cctcgtcga 
      gctttgctt gctggggcg tcgaacggt cttactctc 
      aacctcatg acacctaaa aattacttg gattatgcc 
      tgccggcaa acatctctc cagggatat aaagtcatt 
      ttgcacact ccgatgcgg attcctcga ccaagtcag 
      cagtatttt cgtgttcct ttggatcaa aatgttgtg 
      ggggctgtt caacctgta atgatcagc acttcagat 
      agggttcaa atgtatgac cctaaaaga cggggttgt 
      tattttccc tcagaaagg catttgaag tattttaaa 
      atttatact tctctcaac tgtaaactc gaatgtttg 
      acgaactat acgttaagt cggtgcaaa tgtgtgaat 
      tttttcatg ccaagagag agtggaaca aacatttgt 
      ggcactggc aaaatagcc tgcatgaga gaagctgaa 
      agattttta caggccaaa gatttgtat ttaaaatta 
      tcaaaaaac aaatcattg aagaaaacg agtcaatgc 
      gattgcctc ccgatttgt actgacaca acttatgaa 
      gttgaaact tcacaaagt gattgggat tggaaaatg 
      acctacagt gctcatggc acaccttgg ccaaacgac 
      actcactta tcctctctt acaattttc ttcaaatcg 
      gacactttc atcacatct gaacgaaat gaactctac 
      ggtcctatt gactttttg gcaaatttt ggcggtctc 
      cttggcctt tttacagga ttctctgtg ctttcctta 
      atggaaatt ttgtatttt ctatcagtg agaatcatt 
      tgcaacaat cggttgtac ggtttttgg gcaggcccc 
      agtatttaa acaagttat actaaaaag gtgacttat 
      ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY