| General information | |||
| TC003483 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5844476..5858713 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003483 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08892 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003483 RA
MKKQNVRTL SLIVCTFTY LLIGAAVFD ALESKTENQ RKVVLQEIQ AIVRRKYNI SDEDFRIME TVVLKSEPH RAGQQWKFT GAFYYATTV LTTIGYGHS TPSTIAGKL FTMCYAMIG IPLGLVMFQ SIGERVNKL SSVIIRSVK SSLHCRQTA ASELDLICV VTTLSSLTI AGGAAAFSR YEGWSYFDS VYYCFITLT TIGFGDMVA LQKDNALSK KPEYVMFAL IFILFGLAI VAASLNLLV LRFVTMNTE DERRDEAQA MQALAGAVR LEGDVITAN GSILSGQMG HHCTETTSL DADDTSVCS CHCGCFTSN SRHRSLSRF TVRRSPTQI GHLLPVLPM HELHLPQGK ILPPPAIYP NHRASI
- >TC003483 RB
MKKQNVRTL SLIVCTFTY LLIGAAVFD ALESKTENQ RKVVLQEIQ AIVRRKYNI SDEDFRIME TVVLKSEPH RAGQQWKFT GAFYYATTV LTTIGYGHS TPSTIAGKL FTMCYAMIG IPLGLVMFQ SIGERVNKL SSVIIRSVK SSLHCRQTA ASELDLICV VTTLSSLTI AGGAAAFSR YEGWSYFDS VYYCFITLT TIGFGDMVA LQKDNALSK KPEYVMFAL IFILFGLAI VAASLNLLV LRFVTMNTE DERRDEAQA MQALAGAVR LEGDVITAN GSILSGQMG HHCTETTSL DADDTSVCS CHCGCFTSN SRHRFTVRR SPTQIGHLL PVLPMHELH LPQGKILPP PAIYPNHRA SI
- >TC003483 RA
atgaagaag caaaacgtt cgcacgcta tctcttatc gtttgcacc ttcacgtac ctgctgatc ggcgccgcc gtcttcgac gctcttgaa tctaaaacc gaaaatcaa cgcaaagtc gtcctgcaa gaaatccaa gcaatagtt agaagaaaa tacaacata agcgacgaa gatttcagg ataatggaa actgtcgtc ctgaaatct gagccgcat agagccggc caacaatgg aaattcact ggagccttc tactacgcg actactgtt ctaaccacg ataggttac ggccactct acacccagc accattgcg gggaagctg ttcaccatg tgttacgct atgataggg ataccgcta ggactcgtc atgttccag agcataggc gagagggtc aacaagcta agcagtgtg ataattcgg tccgtgaag tcttccttg cactgccga caaacagca gcctccgag ctggacctc atctgtgtc gtaacaacg ctgagttcg ttgacgata gctggtggt gctgccgcc ttctcgcgt tacgaaggc tggagctac ttcgactcc gtttattat tgcttcatc acactcact actatagga ttcggtgac atggtagca ctccaaaaa gacaatgct ctcagtaaa aaaccggag tatgtcatg tttgcactc atcttcatc ctctttggc ttggccatt gtggcggct tcactgaac ttgctcgtt cttcgcttc gtcacgatg aacacggaa gacgagagg cgggacgag gcgcaagcc atgcaggct ctggctggc gccgtgaga ctcgaaggc gacgtcatc acggccaat ggctcgatt ctgagcggc caaatgggc catcactgt acagaaacc acctcccta gacgctgat gatacctcc gtgtgttct tgtcattgt ggctgtttt acttctaat agtaggcac aggtcgctt tccaggttc acagtacga aggtctccc acgcaaatc gggcatttg ctccccgtt ctgccaatg catgagctt catcttccc caagggaaa atattgccc ccaccggcg atatacccc aaccatagg gcttcgata tga
- >TC003483 RB
atgaagaag caaaacgtt cgcacgcta tctcttatc gtttgcacc ttcacgtac ctgctgatc ggcgccgcc gtcttcgac gctcttgaa tctaaaacc gaaaatcaa cgcaaagtc gtcctgcaa gaaatccaa gcaatagtt agaagaaaa tacaacata agcgacgaa gatttcagg ataatggaa actgtcgtc ctgaaatct gagccgcat agagccggc caacaatgg aaattcact ggagccttc tactacgcg actactgtt ctaaccacg ataggttac ggccactct acacccagc accattgcg gggaagctg ttcaccatg tgttacgct atgataggg ataccgcta ggactcgtc atgttccag agcataggc gagagggtc aacaagcta agcagtgtg ataattcgg tccgtgaag tcttccttg cactgccga caaacagca gcctccgag ctggacctc atctgtgtc gtaacaacg ctgagttcg ttgacgata gctggtggt gctgccgcc ttctcgcgt tacgaaggc tggagctac ttcgactcc gtttattat tgcttcatc acactcact actatagga ttcggtgac atggtagca ctccaaaaa gacaatgct ctcagtaaa aaaccggag tatgtcatg tttgcactc atcttcatc ctctttggc ttggccatt gtggcggct tcactgaac ttgctcgtt cttcgcttc gtcacgatg aacacggaa gacgagagg cgggacgag gcgcaagcc atgcaggct ctggctggc gccgtgaga ctcgaaggc gacgtcatc acggccaat ggctcgatt ctgagcggc caaatgggc catcactgt acagaaacc acctcccta gacgctgat gatacctcc gtgtgttct tgtcattgt ggctgtttt acttctaat agtaggcac aggttcaca gtacgaagg tctcccacg caaatcggg catttgctc cccgttctg ccaatgcat gagcttcat cttccccaa gggaaaata ttgccccca ccggcgata taccccaac catagggct tcgatatga
- >TC003483 RA
gctggcgtc tccacatcc agaagccga gtggattcg gagcaccgt cgtgacgtc actgcgacg tcgttatcg atctcttaa ccgcgtgtt acaacagaa agccccgaa aagctctag tacacggtc ggcggtggt gtttgtgcc aaaatttgc aaagtcgtc ttctgcact gcagcgcac tcgacaatg gttgctgtc cgctcggaa aagagcgta tgtgtctcg tcataaaaa tcgctttgg acgttcctc ctcggtttg cagattacc tcgctattt tagtgcggg gttgtgatg tggttgact ggagggggg tgcgcgggg ggcagctag cggcgtggg agccccccg gccccagca tgacgcgcg ctagagaat gaagaagca aaacgttcg cacgctatc tcttatcgt ttgcacctt cacgtacct gctgatcgg cgccgccgt cttcgacgc tcttgaatc taaaaccga aaatcaacg caaagtcgt cctgcaaga aatccaagc aatagttag aagaaaata caacataag cgacgaaga tttcaggat aatggaaac tgtcgtcct gaaatctga gccgcatag agccggcca acaatggaa attcactgg agccttcta ctacgcgac tactgttct aaccacgat aggttacgg ccactctac acccagcac cattgcggg gaagctgtt caccatgtg ttacgctat gatagggat accgctagg actcgtcat gttccagag cataggcga gagggtcaa caagctaag cagtgtgat aattcggtc cgtgaagtc ttccttgca ctgccgaca aacagcagc ctccgagct ggacctcat ctgtgtcgt aacaacgct gagttcgtt gacgatagc tggtggtgc tgccgcctt ctcgcgtta cgaaggctg gagctactt cgactccgt ttattattg cttcatcac actcactac tataggatt cggtgacat ggtagcact ccaaaaaga caatgctct cagtaaaaa accggagta tgtcatgtt tgcactcat cttcatcct ctttggctt ggccattgt ggcggcttc actgaactt gctcgttct tcgcttcgt cacgatgaa cacggaaga cgagaggcg ggacgaggc gcaagccat gcaggctct ggctggcgc cgtgagact cgaaggcga cgtcatcac ggccaatgg ctcgattct gagcggcca aatgggcca tcactgtac agaaaccac ctccctaga cgctgatga tacctccgt gtgttcttg tcattgtgg ctgttttac ttctaatag taggcacag gtcgctttc caggttcac agtacgaag gtctcccac gcaaatcgg gcatttgct ccccgttct gccaatgca tgagcttca tcttcccca agggaaaat attgccccc accggcgat ataccccaa ccatagggc ttcgatatg atttcctgg ggcataagc ctacatgtt caatgtgat acgtaaaaa ctagataaa agtaatcaa ttcgaacga aacgttagt ggtttttaa tcatcgatt gtgtgtaaa gatgagagt cgtctcatt ttatgaaac aataaccat ataatcgca ctaactgaa acgtcttat gaaacaagt atgaaagca caaatcgat attttttct taaggatta tttctatga tttacgtgt ttctcacaa tcagtgaat ttgttggaa gcgcagttt gtatacaaa ttgatgtga tttcgatgt aaatgccag ctcctagtt tcatccccg agaaggtat ccgttgtct tttccatta ttgtagcgt gatactttg gacacacac aaagttatt atacgggtt gagagtcac ccacgaaat ttgggatca aagtaacgt agttgccaa atttatctc gtttcttat acacttttg aaaacttcc aatatctaa taatatcga gttactaag ccctcaaat cttccatga tttatcaaa tgttaaatg tccacgttt catatctga cggcgaatg caacttttg actgttgtt aagcggaaa ttaaattac agttcgtta tcatttgct tcgtcgaga attcttaca tctgcttct atcaaactt tcgttgact cgaagggaa cataagtag cgttactgt tactaaact aaaacgttt ctaaatagt tttcaaaag tgtgaacaa taagtaaat gttcttccg tttccttga aacagttgc ttctattac ttgccaaaa accgacact agataagtg gtgcttcct gaacaatgc tatttaccg cactgagct taagggcct ctgaaacac ccttaataa atgttaaaa atctgctca agtaccatt gcagtattg tttggttcg aatactcag tattagcat cgcaagatg gactaacgt aaggacaca ctgggcaga ttttcaaag tgcattgta aataaccag actgcattg tacataatc cgagtactg atttagtca tttttgaaa acttagtct tgatttgag attgtacgt agacttttg ataaaaatt gttttaaag aaagaacat gtctaatcg ataaatttc catgatggt tatgaattt taggcctaa cattaacat aacagtatt gcgtagatt ttacacttt taccacaaa acgttgaat atgatatag acatagagt acatttcta aattttaat cgaggtcat tttaataaa gagttgttc tttctgtta aaacttatg taagtaatg tataatttt tgaatcata gtatgtaat tatacttaa aaaatgtgt caaccattt gaaatgtta cctattaat taagcttgt ttttattga aaaattgta actgctgaa cggtttaga taggttcaa gttgctgta aataccaca taaaaatga caaagaata ggttgtaaa ggtgtacgt atacatagt ctttccgcc aaatcactg tcgataatt atttttcga attatatca ccgaacact ttactaaat acccatagg ctaacattt ccacacttc tactgatga aatagattt atgtaaaaa ttgtcatct agtttttta atgtcagtg tttgcatgt tgaacagat agatttacc tcacagagc aagataaaa ctgcgcttt tcttcattt cttgtctta ttatttttc gactgtcat tgtgatgct aaaaacgag cgttaaaaa ggaccatgt gtaaataaa cttttacta aacca
- >TC003483 RB
gctggcgtc tccacatcc agaagccga gtggattcg gagcaccgt cgtgacgtc actgcgacg tcgttatcg atctcttaa ccgcgtgtt acaacagaa agccccgaa aagctctag tacacggtc ggcggtggt gtttgtgcc aaaatttgc aaagtcgtc ttctgcact gcagcgcac tcgacaatg gttgctgtc cgctcggaa aagagcgta tgtgtctcg tcataaaaa tcgctttgg acgttcctc ctcggtttg cagattacc tcgctattt tagtgcggg gttgtgatg tggttgact ggagggggg tgcgcgggg ggcagctag cggcgtggg agccccccg gccccagca tgacgcgcg ctagagaat gaagaagca aaacgttcg cacgctatc tcttatcgt ttgcacctt cacgtacct gctgatcgg cgccgccgt cttcgacgc tcttgaatc taaaaccga aaatcaacg caaagtcgt cctgcaaga aatccaagc aatagttag aagaaaata caacataag cgacgaaga tttcaggat aatggaaac tgtcgtcct gaaatctga gccgcatag agccggcca acaatggaa attcactgg agccttcta ctacgcgac tactgttct aaccacgat aggttacgg ccactctac acccagcac cattgcggg gaagctgtt caccatgtg ttacgctat gatagggat accgctagg actcgtcat gttccagag cataggcga gagggtcaa caagctaag cagtgtgat aattcggtc cgtgaagtc ttccttgca ctgccgaca aacagcagc ctccgagct ggacctcat ctgtgtcgt aacaacgct gagttcgtt gacgatagc tggtggtgc tgccgcctt ctcgcgtta cgaaggctg gagctactt cgactccgt ttattattg cttcatcac actcactac tataggatt cggtgacat ggtagcact ccaaaaaga caatgctct cagtaaaaa accggagta tgtcatgtt tgcactcat cttcatcct ctttggctt ggccattgt ggcggcttc actgaactt gctcgttct tcgcttcgt cacgatgaa cacggaaga cgagaggcg ggacgaggc gcaagccat gcaggctct ggctggcgc cgtgagact cgaaggcga cgtcatcac ggccaatgg ctcgattct gagcggcca aatgggcca tcactgtac agaaaccac ctccctaga cgctgatga tacctccgt gtgttcttg tcattgtgg ctgttttac ttctaatag taggcacag gttcacagt acgaaggtc tcccacgca aatcgggca tttgctccc cgttctgcc aatgcatga gcttcatct tccccaagg gaaaatatt gcccccacc ggcgatata ccccaacca tagggcttc gatatgatt tcctggggc ataagccta catgttcaa tgtgatacg taaaaacta gataaaagt aatcaattc gaacgaaac gttagtggt ttttaatca tcgattgtg tgtaaagat gagagtcgt ctcatttta tgaaacaat aaccatata atcgcacta actgaaacg tcttatgaa acaagtatg aaagcacaa atcgatatt ttttcttaa ggattattt ctatgattt acgtgtttc tcacaatca gtgaatttg ttggaagcg cagtttgta tacaaattg atgtgattt cgatgtaaa tgccagctc ctagtttca tccccgaga aggtatccg ttgtctttt ccattattg tagcgtgat actttggac acacacaaa gttattata cgggttgag agtcaccca cgaaatttg ggatcaaag taacgtagt tgccaaatt tatctcgtt tcttataca cttttgaaa acttccaat atctaataa tatcgagtt actaagccc tcaaatctt ccatgattt atcaaatgt taaatgtcc acgtttcat atctgacgg cgaatgcaa cttttgact gttgttaag cggaaatta aattacagt tcgttatca tttgcttcg tcgagaatt cttacatct gcttctatc aaactttcg ttgactcga agggaacat aagtagcgt tactgttac taaactaaa acgtttcta aatagtttt caaaagtgt gaacaataa gtaaatgtt cttccgttt ccttgaaac agttgcttc tattacttg ccaaaaacc gacactaga taagtggtg cttcctgaa caatgctat ttaccgcac tgagcttaa gggcctctg aaacaccct taataaatg ttaaaaatc tgctcaagt accattgca gtattgttt ggttcgaat actcagtat tagcatcgc aagatggac taacgtaag gacacactg ggcagattt tcaaagtgc attgtaaat aaccagact gcattgtac ataatccga gtactgatt tagtcattt ttgaaaact tagtcttga tttgagatt gtacgtaga cttttgata aaaattgtt ttaaagaaa gaacatgtc taatcgata aatttccat gatggttat gaattttag gcctaacat taacataac agtattgcg tagatttta cacttttac cacaaaacg ttgaatatg atatagaca tagagtaca tttctaaat tttaatcga ggtcatttt aataaagag ttgttcttt ctgttaaaa cttatgtaa gtaatgtat aatttttga atcatagta tgtaattat acttaaaaa atgtgtcaa ccatttgaa atgttacct attaattaa gcttgtttt tattgaaaa attgtaact gctgaacgg tttagatag gttcaagtt gctgtaaat accacataa aaatgacaa agaataggt tgtaaaggt gtacgtata catagtctt tccgccaaa tcactgtcg ataattatt tttcgaatt atatcaccg aacacttta ctaaatacc cataggcta acatttcca cacttctac tgatgaaat agatttatg taaaaattg tcatctagt tttttaatg tcagtgttt gcatgttga acagataga tttacctca cagagcaag ataaaactg cgcttttct tcatttctt gtcttatta tttttcgac tgtcattgt gatgctaaa aacgagcgt taaaaagga ccatgtgta aataaactt ttactaaac ca
