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Gene information
General information
TC034015
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006268,TC006269
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6029993..6035107
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034015
    iBeetle screen iB_08880

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034015 RA
      MKIFFVYLI CCNFLKIRA VPIVTGFSQ PIEPNLGEF 
      PFHASLMQL KPDKTYHSF CGGSLIHPR WVLTAAHCI 
      QLDETSPAF KPGEVFVAL GSIYRSGDK AQVLRVEKL 
      VIHPTYLKT GGRNDIGLV RLAKSALLG RNVKLIQLH 
      TNNKEVLLN QTVFLTGFG IINDLYETP IRLRKAVLH 
      VTNYDKCFS EAQFKNVEI CAASRIAEG KACKGDSGG 
      PLFLTRRGH HVQIGVTSH LTLLPFCRV AFNNSGNFN 
      PIEPTSGEY PFHANLLQV INRNTLFSF CGGSLIHPR 
      WVLTAAHCI QENRHTRRL PPGELRIAL GSVYRSGKK 
      AQILRPIKT IIHPTYLKT DRNDIALIK LEKSAKLTA 
      SVNLIRLHV DNNENLLQK TAFLTGFGY TDDGGPSPK 
      RLRKAILHI ADYKKCSGD PRLRHVEIC GASTIAEGK 
      TCDGDSGGP LFIRKNNKY VQVGITSYL ARSPKCRLS 
      NNNSVYTRV SAYIAWISG ITGINFRKF NQN
    CDS sequence
    • >TC034015 RA
      atgaaaatt tttttcgtt tatttaatt tgttgcaat 
      tttttgaaa attcgggca gtgcccatc gtgaccggt 
      ttttcacaa cctattgaa cccaattta ggcgaattt 
      cccttccat gctagtttg atgcaactc aagcctgat 
      aaaacctat cacagtttt tgcgggggt agcttgatc 
      caccccaga tgggtgcta acagccgca cattgcatt 
      caattggat gagacaagt ccagcgttt aaaccaggt 
      gaggttttt gtggcactg gggagtatt tatcggagc 
      ggagacaag gcccaggtc ctgagggtg gaaaaactg 
      gtgattcac ccaacgtat ttaaaaact ggtggtcgt 
      aacgacatc ggattggtc cgacttgcg aaaagtgcg 
      cttttggga cgtaacgtc aaactcatc caattgcac 
      accaataac aaggaggtg ctgcttaac caaaccgtt 
      tttttgacc ggttttgga atcattaac gatttgtac 
      gaaaccccc atcaggtta cgaaaggcc gttttacac 
      gtcactaac tacgacaag tgtttcagt gaagcacag 
      tttaaaaat gttgagatt tgtgcggct tcgaggatc 
      gctgaagga aaagcttgc aaaggggac agtgggggc 
      ccccttttt ctcacaaga agggggcac catgtccaa 
      attggggtg acgagtcat ttaaccttg ttgccgttc 
      tgtagggtg gctttcaac aattcgggt aattttaac 
      ccgattgag cccacttcg ggggaatac ccattccac 
      gccaactta ttacaggtg ataaaccga aacactttg 
      ttcagtttt tgtggcggc agcttgatt caccccaga 
      tgggtttta accgccgct cattgcatc caagaaaat 
      cgacacact cgtcgtttg ccacctggc gagcttcgc 
      attgcgcta ggtagcgtg tacagaagc ggcaaaaaa 
      gcccaaatt ttgcggccc attaaaaca atcattcac 
      ccaacttac ctaaaaacc gatcgtaac gacatcgct 
      ttaataaaa ctcgagaaa agtgcaaaa ctaaccgcc 
      agtgtgaac ctaatccgg ttacacgtc gacaacaat 
      gagaatttg ctacaaaaa accgctttt ctcaccggg 
      ttcggttac accgacgac ggaggtcca agcccgaaa 
      aggctccga aaggcgatt ttacacata gctgattat 
      aaaaaatgt tcgggggat ccccgactg cgacatgtt 
      gaaatttgt ggcgcctcc acaattgcc gaaggcaaa 
      acttgcgac ggtgacagt ggaggtcct ctctttatt 
      aggaaaaat aataaatat gttcaagtt gggataacg 
      agttatttg gctcggtca ccaaaatgc cggctttct 
      aacaataac tcggtttat acgagagtt tcggcttac 
      attgcttgg atttcggga atcacagga attaatttt 
      aggaaattt aaccaaaac tga
    mRNA sequence
    • >TC034015 RA
      gtcctgtta ttgtttaaa taaaataaa tataataat 
      gattgcttc cgtctcgtc tgattgttg gacgtttgc 
      cgtcgcaga tgaaaattt ttttcgttt atttaattt 
      gttgcaatt ttttgaaaa ttcgggcag tgcccatcg 
      tgaccggtt tttcacaac ctattgaac ccaatttag 
      gcgaatttc ccttccatg ctagtttga tgcaactca 
      agcctgata aaacctatc acagttttt gcgggggta 
      gcttgatcc accccagat gggtgctaa cagccgcac 
      attgcattc aattggatg agacaagtc cagcgttta 
      aaccaggtg aggtttttg tggcactgg ggagtattt 
      atcggagcg gagacaagg cccaggtcc tgagggtgg 
      aaaaactgg tgattcacc caacgtatt taaaaactg 
      gtggtcgta acgacatcg gattggtcc gacttgcga 
      aaagtgcgc ttttgggac gtaacgtca aactcatcc 
      aattgcaca ccaataaca aggaggtgc tgcttaacc 
      aaaccgttt ttttgaccg gttttggaa tcattaacg 
      atttgtacg aaaccccca tcaggttac gaaaggccg 
      ttttacacg tcactaact acgacaagt gtttcagtg 
      aagcacagt ttaaaaatg ttgagattt gtgcggctt 
      cgaggatcg ctgaaggaa aagcttgca aaggggaca 
      gtgggggcc ccctttttc tcacaagaa gggggcacc 
      atgtccaaa ttggggtga cgagtcatt taaccttgt 
      tgccgttct gtagggtgg ctttcaaca attcgggta 
      attttaacc cgattgagc ccacttcgg gggaatacc 
      cattccacg ccaacttat tacaggtga taaaccgaa 
      acactttgt tcagttttt gtggcggca gcttgattc 
      accccagat gggttttaa ccgccgctc attgcatcc 
      aagaaaatc gacacactc gtcgtttgc cacctggcg 
      agcttcgca ttgcgctag gtagcgtgt acagaagcg 
      gcaaaaaag cccaaattt tgcggccca ttaaaacaa 
      tcattcacc caacttacc taaaaaccg atcgtaacg 
      acatcgctt taataaaac tcgagaaaa gtgcaaaac 
      taaccgcca gtgtgaacc taatccggt tacacgtcg 
      acaacaatg agaatttgc tacaaaaaa ccgcttttc 
      tcaccgggt tcggttaca ccgacgacg gaggtccaa 
      gcccgaaaa ggctccgaa aggcgattt tacacatag 
      ctgattata aaaaatgtt cgggggatc cccgactgc 
      gacatgttg aaatttgtg gcgcctcca caattgccg 
      aaggcaaaa cttgcgacg gtgacagtg gaggtcctc 
      tctttatta ggaaaaata ataaatatg ttcaagttg 
      ggataacga gttatttgg ctcggtcac caaaatgcc 
      ggctttcta acaataact cggtttata cgagagttt 
      cggcttaca ttgcttgga tttcgggaa tcacaggaa 
      ttaatttta ggaaattta accaaaact gactagtca 
      ggttggcat taccataaa tattagata atattatta 
      cctagcaaa tttaattta atgatcttt ttctactcc 
      catgtttta actgatcgg ttaaataca gggtgattc 
      tttaggacc tacattata aaattttta acttctaat 
      agctttaaa attttgtgt atgatccaa atcgacaat 
      tcaactaaa ttattttca acatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY