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Gene information
General information
TC033996 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5099323..5106298
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033996
    iBeetle screen iB_08868

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    vitellarium potentially size shortened - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033996 RA
      MDEAPPDAL ILPFDDDQP GTSTLTDSD SDSSTSSLS 
      DDSPKKTTR APRRQATKL DPNLKGLMG EANVRYARG 
      DIETAQKMC FEVMRQVPD AYEPYLTLA QIFENTDST 
      KYEGYLAIA SCLYPQDSG IWCRLAELN IHNEKYKEA 
      VSYYSKAIR ASPRNMGLH LKRLELVEQ IGDVRFALA 
      CKKHMANKL PRAQHQNII ELCKEVAKH YHEEKNYVK 
      ALEVLKIPF KRIPKRVTQ DLVNMMLEL LLLTDRFTE 
      CLDIFIQHC NFTFEITVN EDNTIAIDS YVMPPSIQI 
      DLKVKFIVC LIKLKSFHL VPPLIDSMI REENVEEIG 
      DLYLDVAED LMATNMSLE ALKLLVPLV KSTTYNLAA 
      VWLKYAECL YNCDMLEQA VEAYFTVMT MAPQHVEVL 
      YPLAMTLLK LNKKSEALE VLSQDLSTN KLDVAVLIE 
      RMKLLRQID DIEEYWKCA ELLLSRHCV AVKYPEEAR 
      VVITTSYTV NEKVKKIRD MRRFRGDLM PVESGFEKE 
      IQLFALFST IYNNDTFHG YNLIRNVVT SDSHRHNTL 
      AWNLFNIVL KNTDDIRHN RFISRLQER IPDIHCLQA 
      IEANYSLTT NSNLAIKFY MREFRKLKL PYFALILGV 
      ILLQGYSKR KAVEDRKKL AEIASYLFL YYAKNRSKD 
      AQQEIYYNL GRMYHQLGV MYLAQNYYL KVLEIEEKE 
      PSEILGLRY EAAFNLHLI YKNSGNFTA ARNVLIKNI 
      TI
    • >TC033996 RB
      MDEAPPDAL ILPFDDDQP GTSTLTDSD SDSSTSSLS 
      DDSPKKTTR APRRQATKL DPNLKGLMG EANVRYARG 
      DIETAQKMC FEVMRQVPD AYEPYLTLA QIFENTDST 
      KYEGYLAIA SCLYPQDSG IWCRLAELN IHNEKYKEA 
      VSYYSKAIR ASPRNMGLH LKRLELVEQ IGDVRFALA 
      CKKHMANKL PRAQHQNII ELCKEVAKH YHEEKNYVK 
      ALEVLKIPF KRIPKRVTQ DLVNMMLEL LLLTDRFTE 
      CLDIFIQHC NFTFEITVN EDNTIAIDS YVMPPSIQI 
      DLKVKFIVC LIKLKSFHL VPPLIDSMI REENVEEIG 
      DLYLDVAED LMATNMSLE ALKLLVPLV KSTTYNLAA 
      VWLKYAECL YNCDMLEQA VEAYFTVMT MAPQHVEVL 
      YPLAMTLLK LNKKSEALE VLSQDLSTN KLDVAVLIE 
      RMKLLRQID DIEEYWKCA ELLLSRHCV AVKYPEEAR 
      VVITTSYTV NEKVKKIRD MRRFRGDLM PVESGFEKE 
      IQLFALFST IYNNDTFHG YNLIRNVVT SDSHRHNTL 
      AWNLFNIVL KNTDDIRHN RFISRLQER IPDIHCLQA 
      IEANYSLTT NSNLAIKFY MREFRKLKL PYFALILGV 
      ILLQGYSKR KAVEDRKKL AEIASYLFL YYAKNRSKD 
      AQQEIYYNL GRMYHQLGV ILRRRNQVR YWA
    CDS sequence
    • >TC033996 RA
      atggacgaa gcaccccca gacgcctta atcctcccc 
      tttgacgac gaccagccc ggcaccagc accctcacc 
      gactcggac tccgactcc tccacctca tccctctcc 
      gacgactcc cccaagaaa accacccga gccccccgt 
      cgccaggcc accaaactc gaccccaac ctcaagggc 
      ctcatgggc gaagccaac gtgcgctac gcccgcggc 
      gacatcgaa accgcccag aaaatgtgc ttcgaagtg 
      atgcgccaa gtccccgac gcctacgaa ccctacctg 
      accctggcc cagattttc gaaaacacc gactcgacc 
      aaatacgag gggtacctg gcgattgcc agctgcctg 
      tacccccag gattcgggc atttggtgc cgcttggcg 
      gagctcaac atccacaac gagaagtac aaggaggcg 
      gtttcgtac tactccaag gccattcgg gccagcccc 
      cgcaacatg gggctgcat ctgaagcgg ctggagctc 
      gtggagcag attggcgac gtgcggttt gccctcgcg 
      tgcaagaag cacatggcg aataagttg ccgagggcg 
      cagcaccag aacatcatc gagttgtgc aaggaggtg 
      gcgaagcac taccacgag gagaagaac tacgtcaag 
      gcgctggaa gtgctgaaa atccccttc aaaagaatc 
      cccaagcga gtgacccaa gacttggtg aatatgatg 
      ctggaattg ttgctttta accgatcgt ttcaccgaa 
      tgtttggac attttcatc caacattgc aatttcacg 
      ttcgaaatc accgtaaat gaggataac accattgcg 
      atcgattcg tacgtgatg ccccccagc atccaaatc 
      gacctaaaa gtcaagttt attgtttgc ttgattaaa 
      ctgaagtcg tttcacttg gtgccccct ttaattgac 
      tcaatgata cgcgaggaa aacgttgag gaaatcggt 
      gatttgtac ctggacgtc gctgaggat ctaatggcc 
      acaaatatg tccctcgaa gccctaaaa ctcctagtg 
      ccccttgtc aaaagcacc acttataac ctggctgca 
      gtttggctg aaatacgcc gaatgtctc tacaactgc 
      gacatgttg gagcaggcc gtggaggcc tacttcacg 
      gtcatgaca atggcccca caacacgtc gaagtcctg 
      tacccccta gctatgact ctcctcaaa cttaacaaa 
      aagtccgaa gctttggaa gtgctttcg caggatttg 
      tcgacgaat aagcttgat gtggctgtt cttattgag 
      aggatgaag cttttgcgg caaattgac gatattgag 
      gagtactgg aagtgtgcg gaattgctc ctttccagg 
      cactgtgtt gctgtgaaa tatccagaa gaggcgagg 
      gttgtgatc acaacttcg tatacggtt aatgagaag 
      gtgaagaaa atcagggat atgaggagg tttcggggt 
      gatttgatg ccggttgag tcagggttt gagaaagaa 
      atccagctt ttcgccctc ttctccacc atctacaac 
      aacgacacc ttccacggc tacaacctc atccgcaac 
      gtggtcacc agcgactcc caccgccac aacaccctc 
      gcctggaac ctcttcaac atcgtcctc aaaaacacg 
      gacgacatc cgccacaac cgcttcatc tcacgtctc 
      caagagcgc atccccgac atccactgc ctccaagcg 
      attgaagcc aactacagc cttaccact aactccaac 
      ctcgcgatt aaattctac atgcgagag ttccgcaaa 
      ctcaaactc ccctacttc gctctaatc ctcggcgta 
      atcttacta caaggctac agcaagcga aaagcggtc 
      gaagaccgc aaaaaactc gcagaaatc gcctcatat 
      ctcttccta tattatgcg aaaaaccga agcaaagac 
      gcccaacaa gagatctat tataacttg gggcgaatg 
      taccaccag ctcggcgtc atgtatttg gcccaaaac 
      tattactta aaagtgttg gagattgag gagaaggaa 
      ccaagtgag atactgggc ttgagatac gaagcggca 
      tttaatttg cacttaatt tacaaaaac agcggaaat 
      ttcacagcg gcgaggaac gtgttaata aaaaatatt 
      acgatttga
    • >TC033996 RB
      atggacgaa gcaccccca gacgcctta atcctcccc 
      tttgacgac gaccagccc ggcaccagc accctcacc 
      gactcggac tccgactcc tccacctca tccctctcc 
      gacgactcc cccaagaaa accacccga gccccccgt 
      cgccaggcc accaaactc gaccccaac ctcaagggc 
      ctcatgggc gaagccaac gtgcgctac gcccgcggc 
      gacatcgaa accgcccag aaaatgtgc ttcgaagtg 
      atgcgccaa gtccccgac gcctacgaa ccctacctg 
      accctggcc cagattttc gaaaacacc gactcgacc 
      aaatacgag gggtacctg gcgattgcc agctgcctg 
      tacccccag gattcgggc atttggtgc cgcttggcg 
      gagctcaac atccacaac gagaagtac aaggaggcg 
      gtttcgtac tactccaag gccattcgg gccagcccc 
      cgcaacatg gggctgcat ctgaagcgg ctggagctc 
      gtggagcag attggcgac gtgcggttt gccctcgcg 
      tgcaagaag cacatggcg aataagttg ccgagggcg 
      cagcaccag aacatcatc gagttgtgc aaggaggtg 
      gcgaagcac taccacgag gagaagaac tacgtcaag 
      gcgctggaa gtgctgaaa atccccttc aaaagaatc 
      cccaagcga gtgacccaa gacttggtg aatatgatg 
      ctggaattg ttgctttta accgatcgt ttcaccgaa 
      tgtttggac attttcatc caacattgc aatttcacg 
      ttcgaaatc accgtaaat gaggataac accattgcg 
      atcgattcg tacgtgatg ccccccagc atccaaatc 
      gacctaaaa gtcaagttt attgtttgc ttgattaaa 
      ctgaagtcg tttcacttg gtgccccct ttaattgac 
      tcaatgata cgcgaggaa aacgttgag gaaatcggt 
      gatttgtac ctggacgtc gctgaggat ctaatggcc 
      acaaatatg tccctcgaa gccctaaaa ctcctagtg 
      ccccttgtc aaaagcacc acttataac ctggctgca 
      gtttggctg aaatacgcc gaatgtctc tacaactgc 
      gacatgttg gagcaggcc gtggaggcc tacttcacg 
      gtcatgaca atggcccca caacacgtc gaagtcctg 
      tacccccta gctatgact ctcctcaaa cttaacaaa 
      aagtccgaa gctttggaa gtgctttcg caggatttg 
      tcgacgaat aagcttgat gtggctgtt cttattgag 
      aggatgaag cttttgcgg caaattgac gatattgag 
      gagtactgg aagtgtgcg gaattgctc ctttccagg 
      cactgtgtt gctgtgaaa tatccagaa gaggcgagg 
      gttgtgatc acaacttcg tatacggtt aatgagaag 
      gtgaagaaa atcagggat atgaggagg tttcggggt 
      gatttgatg ccggttgag tcagggttt gagaaagaa 
      atccagctt ttcgccctc ttctccacc atctacaac 
      aacgacacc ttccacggc tacaacctc atccgcaac 
      gtggtcacc agcgactcc caccgccac aacaccctc 
      gcctggaac ctcttcaac atcgtcctc aaaaacacg 
      gacgacatc cgccacaac cgcttcatc tcacgtctc 
      caagagcgc atccccgac atccactgc ctccaagcg 
      attgaagcc aactacagc cttaccact aactccaac 
      ctcgcgatt aaattctac atgcgagag ttccgcaaa 
      ctcaaactc ccctacttc gctctaatc ctcggcgta 
      atcttacta caaggctac agcaagcga aaagcggtc 
      gaagaccgc aaaaaactc gcagaaatc gcctcatat 
      ctcttccta tattatgcg aaaaaccga agcaaagac 
      gcccaacaa gagatctat tataacttg gggcgaatg 
      taccaccag ctcggcgtc atattgagg agaaggaac 
      caagtgaga tactgggct tga
    mRNA sequence
    • >TC033996 RA
      atgtgatgt gcaaacaag cgtaccagt ttatcgatt 
      atttgataa ttttcctgg tgtgaactt ggttggtgt 
      acctgtgtc attagcttt gccgtgcac tgtacccgt 
      gttgtaata tctgtcatc tgccaaaaa cataacctg 
      aaaacaaac cgaaaaaac gttttttct tcgtaacaa 
      caatggacg aagcacccc cagacgcct taatcctcc 
      cctttgacg acgaccagc ccggcacca gcaccctca 
      ccgactcgg actccgact cctccacct catccctct 
      ccgacgact cccccaaga aaaccaccc gagcccccc 
      gtcgccagg ccaccaaac tcgacccca acctcaagg 
      gcctcatgg gcgaagcca acgtgcgct acgcccgcg 
      gcgacatcg aaaccgccc agaaaatgt gcttcgaag 
      tgatgcgcc aagtccccg acgcctacg aaccctacc 
      tgaccctgg cccagattt tcgaaaaca ccgactcga 
      ccaaatacg aggggtacc tggcgattg ccagctgcc 
      tgtaccccc aggattcgg gcatttggt gccgcttgg 
      cggagctca acatccaca acgagaagt acaaggagg 
      cggtttcgt actactcca aggccattc gggccagcc 
      cccgcaaca tggggctgc atctgaagc ggctggagc 
      tcgtggagc agattggcg acgtgcggt ttgccctcg 
      cgtgcaaga agcacatgg cgaataagt tgccgaggg 
      cgcagcacc agaacatca tcgagttgt gcaaggagg 
      tggcgaagc actaccacg aggagaaga actacgtca 
      aggcgctgg aagtgctga aaatcccct tcaaaagaa 
      tccccaagc gagtgaccc aagacttgg tgaatatga 
      tgctggaat tgttgcttt taaccgatc gtttcaccg 
      aatgtttgg acattttca tccaacatt gcaatttca 
      cgttcgaaa tcaccgtaa atgaggata acaccattg 
      cgatcgatt cgtacgtga tgcccccca gcatccaaa 
      tcgacctaa aagtcaagt ttattgttt gcttgatta 
      aactgaagt cgtttcact tggtgcccc ctttaattg 
      actcaatga tacgcgagg aaaacgttg aggaaatcg 
      gtgatttgt acctggacg tcgctgagg atctaatgg 
      ccacaaata tgtccctcg aagccctaa aactcctag 
      tgccccttg tcaaaagca ccacttata acctggctg 
      cagtttggc tgaaatacg ccgaatgtc tctacaact 
      gcgacatgt tggagcagg ccgtggagg cctacttca 
      cggtcatga caatggccc cacaacacg tcgaagtcc 
      tgtaccccc tagctatga ctctcctca aacttaaca 
      aaaagtccg aagctttgg aagtgcttt cgcaggatt 
      tgtcgacga ataagcttg atgtggctg ttcttattg 
      agaggatga agcttttgc ggcaaattg acgatattg 
      aggagtact ggaagtgtg cggaattgc tcctttcca 
      ggcactgtg ttgctgtga aatatccag aagaggcga 
      gggttgtga tcacaactt cgtatacgg ttaatgaga 
      aggtgaaga aaatcaggg atatgagga ggtttcggg 
      gtgatttga tgccggttg agtcagggt ttgagaaag 
      aaatccagc ttttcgccc tcttctcca ccatctaca 
      acaacgaca ccttccacg gctacaacc tcatccgca 
      acgtggtca ccagcgact cccaccgcc acaacaccc 
      tcgcctgga acctcttca acatcgtcc tcaaaaaca 
      cggacgaca tccgccaca accgcttca tctcacgtc 
      tccaagagc gcatccccg acatccact gcctccaag 
      cgattgaag ccaactaca gccttacca ctaactcca 
      acctcgcga ttaaattct acatgcgag agttccgca 
      aactcaaac tcccctact tcgctctaa tcctcggcg 
      taatcttac tacaaggct acagcaagc gaaaagcgg 
      tcgaagacc gcaaaaaac tcgcagaaa tcgcctcat 
      atctcttcc tatattatg cgaaaaacc gaagcaaag 
      acgcccaac aagagatct attataact tggggcgaa 
      tgtaccacc agctcggcg tcatgtatt tggcccaaa 
      actattact taaaagtgt tggagattg aggagaagg 
      aaccaagtg agatactgg gcttgagat acgaagcgg 
      catttaatt tgcacttaa tttacaaaa acagcggaa 
      atttcacag cggcgagga acgtgttaa taaaaaata 
      ttacgattt gagatcttt ttcgaaata ttggcgctc 
      ctttttttc ccgctcgat ggcactcaa catggcgtc 
      gagaaattt ccccaaatc gatgcaagt cccctccag 
      gtaaagcgc atgcgctat gaagggaat tttccttaa 
      agatcggcc gctcaaacc tttactttt ctcgca
    • >TC033996 RB
      atgtgatgt gcaaacaag cgtaccagt ttatcgatt 
      atttgataa ttttcctgg tgtgaactt ggttggtgt 
      acctgtgtc attagcttt gccgtgcac tgtacccgt 
      gttgtaata tctgtcatc tgccaaaaa cataacctg 
      aaaacaaac cgaaaaaac gttttttct tcgtaacaa 
      caatggacg aagcacccc cagacgcct taatcctcc 
      cctttgacg acgaccagc ccggcacca gcaccctca 
      ccgactcgg actccgact cctccacct catccctct 
      ccgacgact cccccaaga aaaccaccc gagcccccc 
      gtcgccagg ccaccaaac tcgacccca acctcaagg 
      gcctcatgg gcgaagcca acgtgcgct acgcccgcg 
      gcgacatcg aaaccgccc agaaaatgt gcttcgaag 
      tgatgcgcc aagtccccg acgcctacg aaccctacc 
      tgaccctgg cccagattt tcgaaaaca ccgactcga 
      ccaaatacg aggggtacc tggcgattg ccagctgcc 
      tgtaccccc aggattcgg gcatttggt gccgcttgg 
      cggagctca acatccaca acgagaagt acaaggagg 
      cggtttcgt actactcca aggccattc gggccagcc 
      cccgcaaca tggggctgc atctgaagc ggctggagc 
      tcgtggagc agattggcg acgtgcggt ttgccctcg 
      cgtgcaaga agcacatgg cgaataagt tgccgaggg 
      cgcagcacc agaacatca tcgagttgt gcaaggagg 
      tggcgaagc actaccacg aggagaaga actacgtca 
      aggcgctgg aagtgctga aaatcccct tcaaaagaa 
      tccccaagc gagtgaccc aagacttgg tgaatatga 
      tgctggaat tgttgcttt taaccgatc gtttcaccg 
      aatgtttgg acattttca tccaacatt gcaatttca 
      cgttcgaaa tcaccgtaa atgaggata acaccattg 
      cgatcgatt cgtacgtga tgcccccca gcatccaaa 
      tcgacctaa aagtcaagt ttattgttt gcttgatta 
      aactgaagt cgtttcact tggtgcccc ctttaattg 
      actcaatga tacgcgagg aaaacgttg aggaaatcg 
      gtgatttgt acctggacg tcgctgagg atctaatgg 
      ccacaaata tgtccctcg aagccctaa aactcctag 
      tgccccttg tcaaaagca ccacttata acctggctg 
      cagtttggc tgaaatacg ccgaatgtc tctacaact 
      gcgacatgt tggagcagg ccgtggagg cctacttca 
      cggtcatga caatggccc cacaacacg tcgaagtcc 
      tgtaccccc tagctatga ctctcctca aacttaaca 
      aaaagtccg aagctttgg aagtgcttt cgcaggatt 
      tgtcgacga ataagcttg atgtggctg ttcttattg 
      agaggatga agcttttgc ggcaaattg acgatattg 
      aggagtact ggaagtgtg cggaattgc tcctttcca 
      ggcactgtg ttgctgtga aatatccag aagaggcga 
      gggttgtga tcacaactt cgtatacgg ttaatgaga 
      aggtgaaga aaatcaggg atatgagga ggtttcggg 
      gtgatttga tgccggttg agtcagggt ttgagaaag 
      aaatccagc ttttcgccc tcttctcca ccatctaca 
      acaacgaca ccttccacg gctacaacc tcatccgca 
      acgtggtca ccagcgact cccaccgcc acaacaccc 
      tcgcctgga acctcttca acatcgtcc tcaaaaaca 
      cggacgaca tccgccaca accgcttca tctcacgtc 
      tccaagagc gcatccccg acatccact gcctccaag 
      cgattgaag ccaactaca gccttacca ctaactcca 
      acctcgcga ttaaattct acatgcgag agttccgca 
      aactcaaac tcccctact tcgctctaa tcctcggcg 
      taatcttac tacaaggct acagcaagc gaaaagcgg 
      tcgaagacc gcaaaaaac tcgcagaaa tcgcctcat 
      atctcttcc tatattatg cgaaaaacc gaagcaaag 
      acgcccaac aagagatct attataact tggggcgaa 
      tgtaccacc agctcggcg tcatattga ggagaagga 
      accaagtga gatactggg cttgagata cgaagcggc 
      atttaattt gcacttaat ttacaaaaa c
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