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Gene information
General information
TC015992 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 192369..196207
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015992
    iBeetle screen iB_08847

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015992 RA
      MITIVIILI FILVSINFI TKYLKKLKQ NKDFYQNIR 
      GPRGLPILG SALDFKTTK DVLQNLINY TRDYGEVVT 
      AQIGPFHRY ILVSNYDFL ECVLSSTKL SSKSHNYNF 
      LRPWLGTGL LTSDGAKWK THRRILTPA FHFQILEQF 
      IEVFEKCGN TLLEKLRNE VDKETCDIY PYVTMCTLD 
      IICESIMGI SINAQEDST SDYVQSVKN VCRIMVERS 
      ISPLQMCPF MYPLTKNYW TEKKSLKIL HKQTNDVIN 
      ARRKELESS KEVSSYEEN FTKKKKPFL DLLLETKID 
      NRLLTQEEI REEVDTFMF EGHDTTASA VSFTLFCLA 
      NNLESQQKA FEEQQAIFG NIQNVTASY TDLQNMKYL 
      EQVIKEALR LYPSVPFYG REITENVEY DGKLLPKGD 
      ILLIFAFGI HRNEKYFPN PEKFDPDRF HNMDNKTPY 
      AYIPFSAGP RNCIGQKFA MLEMKSTVS KVLRQYKLL 
      PTTPQHELE LVGETILKS TNGIKIRVT LRN
    CDS sequence
    • >TC015992 RA
      atgattact attgtcata attttaatt ttcatccta 
      gttagtatt aactttata acaaagtat ttaaaaaaa 
      ttaaaacaa aacaaagac ttttaccaa aacattcgt 
      ggtcctcgt ggtttacca attttagga agtgcttta 
      gatttcaaa actacaaaa gacgtgctt caaaattta 
      atcaactac acaagggat tatggcgag gttgtgacg 
      gcccaaatc ggccctttt caccgatat attctagtg 
      tcgaattac gactttttg gagtgtgtt ttaagcagc 
      acaaaattg agtagcaag tcgcataat tacaacttt 
      cttcgccct tggttgggt accggtttg ttgacgagt 
      gatggagcg aaatggaaa actcacaga cgcatttta 
      accccagct ttccatttt caaattttg gaacaattc 
      atcgaagtt ttcgaaaag tgcggaaac acacttctt 
      gaaaaattg cgaaacgaa gtcgataaa gaaacttgt 
      gatatttat ccgtatgtt acaatgtgc acccttgac 
      ataatttgc gaaagtata atggggata agtataaat 
      gcacaggaa gatagcacc tcggattac gtccaaagt 
      gtgaaaaat gtgtgtcgg attatggtt gaacgatcc 
      atttctcct cttcagatg tgccctttt atgtatccg 
      ttgacaaaa aattactgg accgaaaaa aaatcgcta 
      aaaatcttg cataaacaa accaatgat gtgatcaat 
      gccaggcga aaggaattg gagagttcg aaagaggtc 
      tctagctat gaagaaaat tttacgaaa aagaaaaaa 
      ccattttta gatttgtta ttagaaacg aaaatcgat 
      aaccggttg ttgacacag gaagagata agagaagaa 
      gtggacact ttcatgttt gaaggtcat gacaccaca 
      gcttctgca gtcagcttt actttattt tgtttggct 
      aacaattta gaaagtcag caaaaagcg tttgaggaa 
      cagcaagca atttttggt aacattcaa aatgtgact 
      gcttcatat acggatttg caaaatatg aaatatctg 
      gagcaagtc ataaaggag gctttacgt ttataccca 
      tccgttcca ttttacggc agagaaatc accgaaaat 
      gttgaatat gatggcaag ctattacca aaaggagac 
      attttgtta atttttgcg tttgggata catcgaaac 
      gagaaatat tttccaaat ccggaaaag tttgatcct 
      gacagattc cacaatatg gataataaa acgccctac 
      gcttatata cccttcagt gctggacca agaaactgc 
      attggccaa aaatttgca atgctggaa atgaaaagt 
      acagtctcc aaagtactc agacaatac aaattattg 
      ccaacgacg ccacaacat gagctggaa ctggtagga 
      gaaactatt ttaaaatca actaatgga attaaaata 
      cgtgtaacg ttaaggaat taa
    mRNA sequence
    • >TC015992 RA
      atcgtaagt tgtgtgatc ctttagcat catttaacc 
      aatcaatga gaaaataat gattactat tgtcataat 
      tttaatttt catcctagt tagtattaa ctttataac 
      aaagtattt aaaaaaatt aaaacaaaa caaagactt 
      ttaccaaaa cattcgtgg tcctcgtgg tttaccaat 
      tttaggaag tgctttaga tttcaaaac tacaaaaga 
      cgtgcttca aaatttaat caactacac aagggatta 
      tggcgaggt tgtgacggc ccaaatcgg cccttttca 
      ccgatatat tctagtgtc gaattacga ctttttgga 
      gtgtgtttt aagcagcac aaaattgag tagcaagtc 
      gcataatta caactttct tcgcccttg gttgggtac 
      cggtttgtt gacgagtga tggagcgaa atggaaaac 
      tcacagacg cattttaac cccagcttt ccattttca 
      aattttgga acaattcat cgaagtttt cgaaaagtg 
      cggaaacac acttcttga aaaattgcg aaacgaagt 
      cgataaaga aacttgtga tatttatcc gtatgttac 
      aatgtgcac ccttgacat aatttgcga aagtataat 
      ggggataag tataaatgc acaggaaga tagcacctc 
      ggattacgt ccaaagtgt gaaaaatgt gtgtcggat 
      tatggttga acgatccat ttctcctct tcagatgtg 
      cccttttat gtatccgtt gacaaaaaa ttactggac 
      cgaaaaaaa atcgctaaa aatcttgca taaacaaac 
      caatgatgt gatcaatgc caggcgaaa ggaattgga 
      gagttcgaa agaggtctc tagctatga agaaaattt 
      tacgaaaaa gaaaaaacc atttttaga tttgttatt 
      agaaacgaa aatcgataa ccggttgtt gacacagga 
      agagataag agaagaagt ggacacttt catgtttga 
      aggtcatga caccacagc ttctgcagt cagctttac 
      tttattttg tttggctaa caatttaga aagtcagca 
      aaaagcgtt tgaggaaca gcaagcaat ttttggtaa 
      cattcaaaa tgtgactgc ttcatatac ggatttgca 
      aaatatgaa atatctgga gcaagtcat aaaggaggc 
      tttacgttt atacccatc cgttccatt ttacggcag 
      agaaatcac cgaaaatgt tgaatatga tggcaagct 
      attaccaaa aggagacat tttgttaat ttttgcgtt 
      tgggataca tcgaaacga gaaatattt tccaaatcc 
      ggaaaagtt tgatcctga cagattcca caatatgga 
      taataaaac gccctacgc ttatatacc cttcagtgc 
      tggaccaag aaactgcat tggccaaaa atttgcaat 
      gctggaaat gaaaagtac agtctccaa agtactcag 
      acaatacaa attattgcc aacgacgcc acaacatga 
      gctggaact ggtaggaga aactatttt aaaatcaac 
      taatggaat taaaatacg tgtaacgtt aaggaatta 
      aggcaaaca attcattat aaataattt tgattacta 
      ctcgtattg cttatcaaa ttggaatta ggaataaaa 
      tggcattat tata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY