Status
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Gene information
General information
TC033934 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 60887..109398
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033934
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08846

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033934 RA
      MFVVLLIFV LFQLVESSG ISNETGCFS EPQCLKCGP 
KGCIKCPNL IVHPSRRCV TTCPYGHQP RWSTRLDYM 
GRICVHNGN FLGLPSETA TVLTGVIAG TVLCAILIG 
SAVIYLRIR RKHLPQNID AGSDLDDSP ERRDFLKQL 
ETLKPYAQT YLDMLNDTR RQVRELHRT GDTHALAAY 
KPVLRDLAK ILLLLNRKS DRVAVPDDW EHLFNWAEK 
ALKRYKRMS DVSQPQVAQ LINFLQGPV LPTQDTDSR 
NSTTMSTFK PDQVFGSSL SLQSAAIKN FNSNYDSAL 
HATLNPQWK FEYSLVNSP TSEFNPAVW KNSKEYLNS 
SLFLDDDFC QLGFRPQDE ITTEL
    CDS sequence
    • >TC033934 RA
      atgtttgtt gtgcttttg atttttgtg ctttttcaa 
ctggtggaa tcttcaggg attagcaac gaaacagga 
tgtttctct gaacctcaa tgcctgaaa tgtggcccc 
aaaggatgt atcaaatgt cccaatttg atcgtgcat 
ccgtcccgt cggtgtgtt accacgtgt ccgtacggc 
caccagccc agatggagc acccgtctc gattatatg 
ggtcgcata tgcgtccat aacggcaac ttcctgggt 
ctcccatcg gaaaccgca accgtcctc accggagtc 
attgccgga acagtatta tgcgccatt ctgattggc 
tcagctgtg atctatctt agaattaga cgaaaacac 
ctgccgcaa aatattgac gccggctcg gatttggac 
gattcgccc gaaagacga gacttcctc aaacagctg 
gaaacgttg aagccctac gcgcaaact tacctcgat 
atgttgaat gatacgagg cgacaggtg cgggagctg 
cacaggacg ggagataca cacgctctc gccgcctac 
aagcccgtc ctccgcgac ttggcaaaa atccttctc 
ctcctcaac cgcaaatcg gaccgtgtg gcagtccct 
gacgactgg gagcacctc ttcaactgg gccgaaaag 
gccctcaaa cgctacaag cgaatgagc gacgtctcg 
cagccccaa gtcgcccaa ctaatcaac ttcctgcag 
ggccctgta ttaccaacg caggacacc gacagcagg 
aacagcacc acaatgtcc accttcaaa cccgaccaa 
gtgtttggg tcttctcta agtctgcaa agtgcggca 
ataaagaat tttaatagt aattatgat tctgcactt 
catgccact ctgaatccg cagtggaag ttcgagtat 
tcgctcgtt aattcgccc acgtcggag ttcaatccg 
gcggtttgg aagaacagt aaagagtac cttaacagt 
tcgttgttc ttggacgat gatttctgc caattgggt 
ttcaggcct caggatgag attacaact gaattgtga
    mRNA sequence
    • >TC033934 RA
      ccgagctgg tgtttcttt gtaaagtgt tggtagttc 
gttggtacc tatttttgt gacctgcac ccccccatt 
tcgagaaga attttctaa ccgcacatt cggacttaa 
aagggaccg tttcagaaa ttctttaaa aatcaataa 
ctggtggca atatgataa cgaagtcca tcttataac 
actgaggcc cgcatctta taacaacat ccagtctgc 
cgaaaaaat tctctcatg ctccaacaa tgcaataaa 
accaaaaca cgtcttaca ctattttgt caattttga 
aaattaatc ttaattgtc ttgaaaatc ggggtccta 
ggtcaatgc caataactt gaaaaaaac acaattacg 
tggctaatc cgagcaatt atagtacaa atctagcaa 
aaggcccca ggaggccta caagcaaca ggttctttt 
gttgtgttc acgtgatcg gaggtaccc ggagttgta 
gcgtgtcct ccgaagtga agtgaatta gtagctgtt 
gagcagtgg gtgatgcaa gttgtgttg gtgctgtgc 
tcggaatgg ttccgtggt agtgcgaga gtgacttag 
aatggtact gctaaaaca tgtttgttg tgcttttga 
tttttgtgc tttttcaac tggtggaat cttcaggga 
ttagcaacg aaacaggat gtttctctg aacctcaat 
gcctgaaat gtggcccca aaggatgta tcaaatgtc 
ccaatttga tcgtgcatc cgtcccgtc ggtgtgtta 
ccacgtgtc cgtacggcc accagccca gatggagca 
cccgtctcg attatatgg gtcgcatat gcgtccata 
acggcaact tcctgggtc tcccatcgg aaaccgcaa 
ccgtcctca ccggagtca ttgccggaa cagtattat 
gcgccattc tgattggct cagctgtga tctatctta 
gaattagac gaaaacacc tgccgcaaa atattgacg 
ccggctcgg atttggacg attcgcccg aaagacgag 
acttcctca aacagctgg aaacgttga agccctacg 
cgcaaactt acctcgata tgttgaatg atacgaggc 
gacaggtgc gggagctgc acaggacgg gagatacac 
acgctctcg ccgcctaca agcccgtcc tccgcgact 
tggcaaaaa tccttctcc tcctcaacc gcaaatcgg 
accgtgtgg cagtccctg acgactggg agcacctct 
tcaactggg ccgaaaagg ccctcaaac gctacaagc 
gaatgagcg acgtctcgc agccccaag tcgcccaac 
taatcaact tcctgcagg gccctgtat taccaacgc 
aggacaccg acagcagga acagcacca caatgtcca 
ccttcaaac ccgaccaag tgtttgggt cttctctaa 
gtctgcaaa gtgcggcaa taaagaatt ttaatagta 
attatgatt ctgcacttc atgccactc tgaatccgc 
agtggaagt tcgagtatt cgctcgtta attcgccca 
cgtcggagt tcaatccgg cggtttgga agaacagta 
aagagtacc ttaacagtt cgttgttct tggacgatg 
atttctgcc aattgggtt tcaggcctc aggatgaga 
ttacaactg aattgtgac gacaatttc gatttttgg 
cgtcactgt taaaccaaa ttctattac tagtcatgt 
attttttac cttgtatgt taagtggaa tttatagtt 
ttaagtgaa cccagtgta catttttta atgtaaatt 
gattaactt tgatttgat cgcaatttt tgggattgt 
gttttaaga tatgcaaag gttaaatga atgtagcgc 
gtgtcgcaa atattggta caatttgca tatctttta 
atctatctc cgagttgtt aggagtaaa ataattgat 
tatttaaat aaaattatt tatgtagg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY