Status
Please wait ...
Gene information
General information
TC033919 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 639148..645119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033919
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033919 RA
      MIDNEMFAK TAIIFILMK FVCSDNGNS IIQLPNGKI 
      LGREAVTFD KKSYYAFEK IPYATPPLG SLRFKAPQS 
      AQNWDGILN TTHMDVTCM QVTKNLSKE SEDCLYLNV 
      YTPQLPHND NVSFPVMLY IHGGAFMHE SSMDSAPDL 
      FLNNDIIFV SINYRLGPF GFLSTQDEV IPGNNGLKD 
      QLLAIRWTH DNIIYFGGD PGRIIISGE SAGSASVAY 
      QLLNPHSQD LFQGAILES GSCLSPWSY QRNSRNIAF 
      ATAAILNST FETNNDSQA LLEFLQNVD AQELDAAAQ 
      KYLNKVSTP WDYDVSQGC VWAPVIEPK NPDAFLTKK 
      MYGLVKAGN IAKVPILMG FNSEESLYF NKDPDTFKS 
      SMETWDKNL DIIVPNDMQ ITDQEQRVK VGQAIREIY 
      TGGQPFADN LGAGIRYSS DTSFTRPIR KHAEFASSF 
      TQIFFYQFS YDGILGNIN VHYDGAENV GHTEIIKYL 
      FCSGSKCDI SENPENDQI TRNRLIKLW TDFAKYLNP 
      TPEPSQLLQ NITWPSIST DHGDFPYLD INEKLEIKK 
      FPKEETFAK WIELYNDLD YKDFDTY
    CDS sequence
    • >TC033919 RA
      atgattgat aacgaaatg tttgccaaa actgccata 
      atttttatt ttaatgaaa tttgtttgt tccgataat 
      ggaaattct ataatccag ttacccaat ggcaaaatt 
      ttaggtcgc gaagctgta acatttgat aaaaagagc 
      tactatgct tttgaaaag attccttat gccactcct 
      ccactagga tcactgcga tttaaagca ccacaatct 
      gctcaaaat tgggatgga attcttaat acaactcat 
      atggatgtt acttgtatg caagtaaca aagaactta 
      tcgaaggaa tctgaagat tgtctctat ctcaatgtc 
      tacactcct caacttcct cacaatgac aatgtttcg 
      tttccggta atgctatac atacatggg ggtgccttt 
      atgcatgag tcaagtatg gattccgcc cccgatctg 
      tttctcaat aacgatatt atttttgta tctattaat 
      tatagatta ggacctttt ggtttcctt tcaacgcaa 
      gatgaagtc attccaggt aacaatggt ttaaaagat 
      caacttcta gcaatacga tggacacat gacaatatc 
      atatatttt ggaggtgat cctgggagg ataattatt 
      tcaggagag agtgctggt tcagctagt gtggcttat 
      caactctta aatccacac tcacaggac ttatttcaa 
      ggggctatt ttagaaagc ggctcatgt ttgagtcct 
      tggtcatat caaaggaat tccagaaac attgctttt 
      gcaactgct gccattttg aattcaaca tttgaaaca 
      aacaacgat tcacaagct ttattggaa tttctgcaa 
      aatgttgat gctcaggag ttagatgca gcggcacaa 
      aaatatctt aacaaggta agtactcca tgggattac 
      gacgtatcg caaggatgt gtttgggct cctgtaatt 
      gaacccaaa aatccagat gcttttctt actaaaaaa 
      atgtatgga ttggttaaa gccggaaac attgcaaag 
      gtaccaata cttatggga ttcaattct gaagaaagt 
      ctctacttt aataaagat ccagacaca ttcaaatct 
      tcgatggaa acttgggac aaaaacttg gatataata 
      gtcccgaat gacatgcaa attaccgat caagaacaa 
      cgcgtaaaa gtgggccaa gcgattaga gaaatatac 
      acaggaggt caaccgttt gctgataat ttgggagct 
      ggaattaga tactctagt gatacctcc tttactcga 
      cctattcgc aaacacgcc gaatttgct tcgtctttt 
      actcaaata ttcttttat cagttctct tatgatgga 
      atattggga aacattaat gttcattat gatggtgca 
      gaaaatgtt ggccataca gagattata aagtatttg 
      ttttgttca ggatcaaag tgtgatatt agtgagaat 
      ccagaaaac gatcaaatt acgagaaac cggctcatc 
      aaattatgg actgatttt gcaaagtat ttaaatcca 
      acaccggaa ccatcacaa ttattgcaa aatattacc 
      tggccaagt atttccact gatcatggt gactttccc 
      tatctggat attaatgaa aaattggaa ataaaaaaa 
      tttccaaaa gaagaaact tttgcaaaa tggatcgaa 
      ctttataat gatttggac tacaaagat tttgacacc 
      tactaa
    mRNA sequence
    • >TC033919 RA
      ttttatgaa aaccaatgt tttgttgta aataattgt 
      tttatgcaa tggcaccgc aacgaaggc gaatcccct 
      cttttattc acttaggta ccaagttaa tccaaaacc 
      aacacgcag tgtaacaaa acaaagtta attgtttag 
      gtatcattt gctcactta aatctagat aatcaaatg 
      attgataac gaaatgttt gccaaaact gccataatt 
      tttatttta atgaaattt gtttgttcc gataatgga 
      aattctata atccagtta cccaatggc aaaatttta 
      ggtcgcgaa gctgtaaca tttgataaa aagagctac 
      tatgctttt gaaaagatt ccttatgcc actcctcca 
      ctaggatca ctgcgattt aaagcacca caatctgct 
      caaaattgg gatggaatt cttaataca actcatatg 
      gatgttact tgtatgcaa gtaacaaag aacttatcg 
      aaggaatct gaagattgt ctctatctc aatgtctac 
      actcctcaa cttcctcac aatgacaat gtttcgttt 
      ccggtaatg ctatacata catgggggt gcctttatg 
      catgagtca agtatggat tccgccccc gatctgttt 
      ctcaataac gatattatt tttgtatct attaattat 
      agattagga ccttttggt ttcctttca acgcaagat 
      gaagtcatt ccaggtaac aatggttta aaagatcaa 
      cttctagca atacgatgg acacatgac aatatcata 
      tattttgga ggtgatcct gggaggata attatttca 
      ggagagagt gctggttca gctagtgtg gcttatcaa 
      ctcttaaat ccacactca caggactta tttcaaggg 
      gctatttta gaaagcggc tcatgtttg agtccttgg 
      tcatatcaa aggaattcc agaaacatt gcttttgca 
      actgctgcc attttgaat tcaacattt gaaacaaac 
      aacgattca caagcttta ttggaattt ctgcaaaat 
      gttgatgct caggagtta gatgcagcg gcacaaaaa 
      tatcttaac aaggtaagt actccatgg gattacgac 
      gtatcgcaa ggatgtgtt tgggctcct gtaattgaa 
      cccaaaaat ccagatgct tttcttact aaaaaaatg 
      tatggattg gttaaagcc ggaaacatt gcaaaggta 
      ccaatactt atgggattc aattctgaa gaaagtctc 
      tactttaat aaagatcca gacacattc aaatcttcg 
      atggaaact tgggacaaa aacttggat ataatagtc 
      ccgaatgac atgcaaatt accgatcaa gaacaacgc 
      gtaaaagtg ggccaagcg attagagaa atatacaca 
      ggaggtcaa ccgtttgct gataatttg ggagctgga 
      attagatac tctagtgat acctccttt actcgacct 
      attcgcaaa cacgccgaa tttgcttcg tcttttact 
      caaatattc ttttatcag ttctcttat gatggaata 
      ttgggaaac attaatgtt cattatgat ggtgcagaa 
      aatgttggc catacagag attataaag tatttgttt 
      tgttcagga tcaaagtgt gatattagt gagaatcca 
      gaaaacgat caaattacg agaaaccgg ctcatcaaa 
      ttatggact gattttgca aagtattta aatccaaca 
      ccggaacca tcacaatta ttgcaaaat attacctgg 
      ccaagtatt tccactgat catggtgac tttccctat 
      ctggatatt aatgaaaaa ttggaaata aaaaaattt 
      ccaaaagaa gaaactttt gcaaaatgg atcgaactt 
      tataatgat ttggactac aaagatttt gacacctac 
      taaccacaa cacgtagac acactaaat acattctta 
      caagtgaag tgtatttcg gttaaattt tagttagat 
      ggttatgct tttaaatca ccctgtatg tgtaatatg 
      ttttaggcg acatctact taattattt taatagaat 
      ttttatgta accaccata aattctgtt gataaaaat 
      tgatctaca aaatattat aaaatacat gtaatgaca 
      ataaagtaa ttccattag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY