Status
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Gene information
General information
TC016108 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451646.1: 212005..221956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016108
    iBeetle screen iB_08840

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016108 RA
      MSSAICQNF VQNAWKKEL CSNCFKSKD EHAGRPKPK 
      PITLISSEK VEGIIRNAS KFRPKRTVC FTKELTEII 
      GFGGEDWES EGEDCKDDD LSSSEEDFI AESEEEEER 
      ELKRITKEN TDFNTNNLK DEVVVKKTT QLLLGKPLV 
      DASGRKQTL LVSVTPFGE DTQPKKIIK SLKLKESVP 
      ETKQTNVVL TSYTKNEDK EEKSLLDEI TETLENGKN 
      PIQIMSRKK TQKEITLTV SKDEPKPER KTTLSRTPA 
      LKRDHEKPL IYQTSTAKI ELKPKIDLK PTPPTTIVP 
      PPPPKSSRE QAGEPDGRA DPDTITEPP ALPLTPPPA 
      LEPPTFLYD KPKIPAKPV LTKTNSNGS DAGSRVINK 
      RKAPKPPEV PEPAPRKFL SVSTDSLAS EDKRKKGRF 
      SLKKFLRRG SSKVDLKGG EEVDGPPLP KPRLVIVHP 
      SELNGARVE VVPKGDYQN IRYSDGNDY ESFVPKKPP 
      PPPRDDLGS YKPLPHPPK SVDVLIKQK QYSGGVPTK 
      KTETVYANI GEVRSAIVP NKPQRTASM REREAQEKK 
      NYESVDGGE NVYDYISGR SSSPDSDSS PNKNSPTSK 
      TARLSKRSE SSIDVSGEY FKYGNIPRS VSLTYCGSE 
      TESEIYSPY SFYGSESEV TEDDHDWIQ NGRTHKLRS 
      RKGRSIVHK NLEDNYGAV IVANHEALA QVLENIQQT 
      THIQPALRG LKTLSNLRW TDFSVKSTS QPLVVGCRT 
      FHQAIWNTQ HVTLVVNTG AVPSSTLTL GTSSLMTIT 
      EFSDLVPAK FVGNESEEE KLVQATVAV LPWLQVSTI 
      QSYGESLKK GAQTLEEAW KDASFIMLQ LVNALKILQ 
      AQGIEELLL SLNSFVLCK EMDKDSHQR LCVLQGLGE 
      DLHLASTDQ YGTLCTCAS KALSLLQPT AKTTPLLQS 
      LLNNERAVT LTQVKSILE FCLWGPSDV ALGSTIRER 
      ELALQRWLD LQRATVLHG LVCARVQLT VYEECHLFF 
      LVRSNARMM CDASLLLES SKHK
    CDS sequence
    • >TC016108 RA
      atgtcttca gcaatctgc caaaatttc gtccaaaat 
      gcgtggaag aaggaactg tgttcgaat tgcttcaaa 
      tcgaaggat gaacacgca ggacggcca aaacctaaa 
      ccaatcaca ctaatttcc agcgaaaaa gtcgaagga 
      atcatcaga aacgcgtcg aaatttcga ccgaaacga 
      accgtttgt tttacgaag gaattgacg gagattatc 
      ggttttggg ggcgaagat tgggagtcg gagggtgag 
      gattgcaaa gacgatgat ttgtcgtcc agtgaagag 
      gattttatt gccgaatct gaggaagaa gaagaacgg 
      gaattgaaa aggataacg aaagaaaac accgatttt 
      aacacaaat aatttgaag gatgaagtt gtggtgaag 
      aaaacaacc cagcttttg ttagggaaa cccctagtt 
      gatgcgtcc ggacgtaaa caaaccttg ctggtttcg 
      gttacacca ttcggtgaa gacacccaa cccaaaaaa 
      ataatcaaa agtttgaaa ttgaaagag agtgtgcct 
      gagacgaaa caaactaac gttgttcta acttcttac 
      acgaagaat gaagataag gaagaaaaa tccttactg 
      gatgaaata accgagact ttggaaaac ggaaagaac 
      ccgatccaa atcatgagt cgcaaaaaa acccaaaaa 
      gaaatcacc ctaactgtc tcaaaggac gaaccgaaa 
      cccgagcga aaaactaca ctttcccgc acgcctgcc 
      ttgaaacgc gaccacgaa aaaccccta atttaccaa 
      acctccact gccaaaatc gagctcaag cctaaaatc 
      gacttgaaa ccaactccc cccaccacc atcgtacca 
      ccccctcca ccaaaatcc agtcgtgag caagcgggc 
      gaacccgac ggcagagcc gaccctgat acaatcacc 
      gaacctcca gccctgccc ttgactccg ccccctgcc 
      ctagaacca cccactttt ctctacgac aaacccaaa 
      attccagct aaaccggtt ttgactaaa acaaactct 
      aacggttcg gatgccggc tctcgtgtt ataaataaa 
      cgaaaagcg ccgaaacca cccgaggtg cctgaacca 
      gccccacgt aagtttctt tcggtttcg accgatagt 
      ttagcgagt gaggataaa agaaaaaag gggaggttt 
      tcactgaag aagtttcta cggagaggg tcgagtaaa 
      gtcgattta aagggtggg gaggaagtg gatggaccc 
      cctttgccc aaaccgaga ctagtaatc gtccatccc 
      tctgaatta aatggggct agagtcgaa gttgtgcca 
      aaaggtgat taccagaac ataaggtat tctgatggt 
      aacgactat gaaagtttc gtaccgaaa aaaccacca 
      ccgccccct cgtgacgat ttagggtcg tataagccg 
      ctcccccac cctccaaaa tcagtcgat gtgcttatt 
      aagcagaaa caatactcc gggggtgtc ccgacgaag 
      aaaactgaa actgtatat gcgaatata ggggaagtc 
      cggtcagcg attgttccg aataagccg caaagaacg 
      gcgagtatg cgtgagagg gaagctcaa gagaagaaa 
      aattatgag tctgtggat ggaggagag aatgtttat 
      gattatatt agtgggagg tcaagttcg cccgattct 
      gattctagt ccgaataaa aatagtccg acgagtaag 
      actgccagg ttgagtaag aggtcagag agtagtatt 
      gatgtgtcc ggggagtat ttcaagtat gggaatatt 
      ccaagatcg gtctcgttg acgtattgt gggagtgag 
      accgagtcg gagatttat tcaccgtat agcttttat 
      ggcagcgag tcagaggtg accgaagac gaccacgac 
      tggatccaa aacggtcgc actcacaaa ctgcgttcg 
      cgtaaaggc cgaagcatc gtccacaaa aacctagaa 
      gacaactat ggagccgtc attgtcgcc aaccatgaa 
      gcactagca caagtcttg gaaaacatc caacaaacc 
      actcacatt cagccggca ctcagaggt ctgaaaacc 
      ctgtccaat ctgcgatgg actgatttt tccgtaaaa 
      tctacgtct cagcctctc gttgtcggc tgtcgaacc 
      ttccatcaa gcaatttgg aacacccag cacgtgact 
      cttgtagtc aacactggg gcggtacca tcaagtacc 
      cttactttg ggtacaagc agtttgatg acaattacc 
      gaatttagt gatttggtg ccggcgaaa tttgtagga 
      aatgaaagt gaggaggag aagttggtt caagcaact 
      gtagcagtt ttgccttgg ttgcaagtc agtacgatt 
      cagtcgtac ggtgagagt ctgaaaaag ggggcgcaa 
      actcttgaa gaagcctgg aaagatgca agttttatt 
      atgttgcag cttgtgaat gccttgaag attttgcaa 
      gctcagggg attgaggag ttgttgctg tcgctaaac 
      agttttgtg ctttgtaaa gagatggat aaagatagt 
      catcagaga ttgtgcgtc ttacaaggc ctcggcgaa 
      gacctccac ctcgcgtcc accgaccaa tacggcacg 
      ctctgcacg tgcgcctcc aaagccctc tccctcctc 
      cagcccacc gccaaaacc acccccctc ctccaatcc 
      ctcctcaac aacgagcgc gccgtcacc ctcacccag 
      gtcaaatcg atcctcgag ttctgcctc tggggcccc 
      tcggacgtc gcccttggg agcaccatc cgcgaacgc 
      gaactcgcc ctccaacgc tggctcgac ctccagcgc 
      gccaccgtc ctccacggc ctcgtgtgc gcacgcgtg 
      caacttacc gtctacgaa gagtgccat ctgttcttc 
      ttagtgcga agcaacgca aggatgatg tgcgacgcc 
      tctctctta ctcgaatcg tccaaacat aaatga
    mRNA sequence
    • >TC016108 RA
      gtaattaag gctttaatg tggtagcct ttttcgaac 
      tgtgaacat caccatgtc ttcagcaat ctgccaaaa 
      tttcgtcca aaatgcgtg gaagaagga actgtgttc 
      gaattgctt caaatcgaa ggatgaaca cgcaggacg 
      gccaaaacc taaaccaat cacactaat ttccagcga 
      aaaagtcga aggaatcat cagaaacgc gtcgaaatt 
      tcgaccgaa acgaaccgt ttgttttac gaaggaatt 
      gacggagat tatcggttt tgggggcga agattggga 
      gtcggaggg tgaggattg caaagacga tgatttgtc 
      gtccagtga agaggattt tattgccga atctgagga 
      agaagaaga acgggaatt gaaaaggat aacgaaaga 
      aaacaccga ttttaacac aaataattt gaaggatga 
      agttgtggt gaagaaaac aacccagct tttgttagg 
      gaaacccct agttgatgc gtccggacg taaacaaac 
      cttgctggt ttcggttac accattcgg tgaagacac 
      ccaacccaa aaaaataat caaaagttt gaaattgaa 
      agagagtgt gcctgagac gaaacaaac taacgttgt 
      tctaacttc ttacacgaa gaatgaaga taaggaaga 
      aaaatcctt actggatga aataaccga gactttgga 
      aaacggaaa gaacccgat ccaaatcat gagtcgcaa 
      aaaaaccca aaaagaaat caccctaac tgtctcaaa 
      ggacgaacc gaaacccga gcgaaaaac tacactttc 
      ccgcacgcc tgccttgaa acgcgacca cgaaaaacc 
      cctaattta ccaaacctc cactgccaa aatcgagct 
      caagcctaa aatcgactt gaaaccaac tccccccac 
      caccatcgt accaccccc tccaccaaa atccagtcg 
      tgagcaagc gggcgaacc cgacggcag agccgaccc 
      tgatacaat caccgaacc tccagccct gcccttgac 
      tccgccccc tgccctaga accacccac ttttctcta 
      cgacaaacc caaaattcc agctaaacc ggttttgac 
      taaaacaaa ctctaacgg ttcggatgc cggctctcg 
      tgttataaa taaacgaaa agcgccgaa accacccga 
      ggtgcctga accagcccc acgtaagtt tctttcggt 
      ttcgaccga tagtttagc gagtgagga taaaagaaa 
      aaaggggag gttttcact gaagaagtt tctacggag 
      agggtcgag taaagtcga tttaaaggg tggggagga 
      agtggatgg acccccttt gcccaaacc gagactagt 
      aatcgtcca tccctctga attaaatgg ggctagagt 
      cgaagttgt gccaaaagg tgattacca gaacataag 
      gtattctga tggtaacga ctatgaaag tttcgtacc 
      gaaaaaacc accaccgcc ccctcgtga cgatttagg 
      gtcgtataa gccgctccc ccaccctcc aaaatcagt 
      cgatgtgct tattaagca gaaacaata ctccggggg 
      tgtcccgac gaagaaaac tgaaactgt atatgcgaa 
      tatagggga agtccggtc agcgattgt tccgaataa 
      gccgcaaag aacggcgag tatgcgtga gagggaagc 
      tcaagagaa gaaaaatta tgagtctgt ggatggagg 
      agagaatgt ttatgatta tattagtgg gaggtcaag 
      ttcgcccga ttctgattc tagtccgaa taaaaatag 
      tccgacgag taagactgc caggttgag taagaggtc 
      agagagtag tattgatgt gtccgggga gtatttcaa 
      gtatgggaa tattccaag atcggtctc gttgacgta 
      ttgtgggag tgagaccga gtcggagat ttattcacc 
      gtatagctt ttatggcag cgagtcaga ggtgaccga 
      agacgacca cgactggat ccaaaacgg tcgcactca 
      caaactgcg ttcgcgtaa aggccgaag catcgtcca 
      caaaaacct agaagacaa ctatggagc cgtcattgt 
      cgccaacca tgaagcact agcacaagt cttggaaaa 
      catccaaca aaccactca cattcagcc ggcactcag 
      aggtctgaa aaccctgtc caatctgcg atggactga 
      tttttccgt aaaatctac gtctcagcc tctcgttgt 
      cggctgtcg aaccttcca tcaagcaat ttggaacac 
      ccagcacgt gactcttgt agtcaacac tggggcggt 
      accatcaag tacccttac tttgggtac aagcagttt 
      gatgacaat taccgaatt tagtgattt ggtgccggc 
      gaaatttgt aggaaatga aagtgagga ggagaagtt 
      ggttcaagc aactgtagc agttttgcc ttggttgca 
      agtcagtac gattcagtc gtacggtga gagtctgaa 
      aaagggggc gcaaactct tgaagaagc ctggaaaga 
      tgcaagttt tattatgtt gcagcttgt gaatgcctt 
      gaagatttt gcaagctca ggggattga ggagttgtt 
      gctgtcgct aaacagttt tgtgctttg taaagagat 
      ggataaaga tagtcatca gagattgtg cgtcttaca 
      aggcctcgg cgaagacct ccacctcgc gtccaccga 
      ccaatacgg cacgctctg cacgtgcgc ctccaaagc 
      cctctccct cctccagcc caccgccaa aaccacccc 
      cctcctcca atccctcct caacaacga gcgcgccgt 
      caccctcac ccaggtcaa atcgatcct cgagttctg 
      cctctgggg cccctcgga cgtcgccct tgggagcac 
      catccgcga acgcgaact cgccctcca acgctggct 
      cgacctcca gcgcgccac cgtcctcca cggcctcgt 
      gtgcgcacg cgtgcaact taccgtcta cgaagagtg 
      ccatctgtt cttcttagt gcgaagcaa cgcaaggat 
      gatgtgcga cgcctctct cttactcga atcgtccaa 
      acataaatg acacaaatt cgttctcaa atgtttatt 
      tttacacgg tgtgcaggc atttgttaa tgttaaaaa 
      ttttattgt atatattgt tattgtaac tgccatttc 
      gaggttttt gtgttattg tcctttttt atacacttc 
      atatactat attatacac tataaataa attaaaaat 
      gctttataa tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY