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Gene information
General information
TC030127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451646.1: 78109..79712
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030127
    iBeetle screen iB_08839

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030127 RA
      MQVASFTAL GFRGTADFV IACFDVNDV IVSAIFFVT 
      STPFKFKHF VQIVENFDR IDARISPIL VEQIRKRSN 
      IFVKVLVTF LPTLYVLDL FMWGKNNWE GLNNYFAFY 
      IMYSIVVVH ELQYWHIMT MMYARILGL NKTLRDYFK 
      NKTGFCEHE ILVVTQSFN SINDSVEEI NKCFSYSTT 
      TIIFSCYIH LVISPYQLF VVVSSTETS LFNYVYLLW 
      ISLHIMRVL TIVEVCQKC ENENRKTRS LVYQLLLCK 
      LNEKVKNMV RVLFFLVTT RKILFSAYA LPKINRRLI 
      ISILSSIST YWMILMQST SRTIQVV
    CDS sequence
    • >TC030127 RA
      atgcaagtg gcttccttc acagccttg ggcttcaga 
      ggcactgct gattttgtg attgcttgc ttcgatgtc 
      aatgatgtg attgttagt gcgattttt ttcgtcact 
      tccacacct ttcaaattt aagcatttt gtgcaaatc 
      gtcgagaat tttgaccga attgacgct cgcatctct 
      ccaatcttg gtggaacag attcgaaaa aggagcaat 
      atttttgta aaagtcttg gtaactttt ttgccaact 
      ttatacgtc cttgatctg tttatgtgg gggaaaaac 
      aactgggag ggattaaat aattatttt gctttttac 
      ataatgtac agcattgtt gtcgttcac gaattgcag 
      tattggcat atcatgact atgatgtat gcccgtatt 
      ttggggctc aataaaact ttgagagat tactttaaa 
      aataaaaca ggattctgc gaacatgaa atattagtg 
      gtcacccaa agttttaat tcgatcaat gattcggtg 
      gaagaaatt aataaatgt tttagttac agtacgact 
      acaattatt ttcagctgt tatattcat ttagtaata 
      agtccgtac caacttttt gtggtagtc tcaagcact 
      gaaacttct ctatttaac tatgtgtat ttactgtgg 
      atctcttta cacattatg agagtttta acaattgtc 
      gaagtttgt cagaaatgt gaaaatgag aaccgaaaa 
      actagaagt ttagtttat caattatta ctttgtaaa 
      ttaaacgaa aaagtcaaa aacatggtt agagtactt 
      ttctttttg gtaacaact agaaaaatc ttgttttct 
      gcatatgct ttgccgaaa attaacaga agacttatc 
      atttccatt cttagttct atcagtact tactggatg 
      attttgatg caatccact tccagaaca atacaagtg 
      gtgtaa
    mRNA sequence
    • >TC030127 RA
      caattacgg aaaatcatt tccacatac acaatttat 
      gtattcaca aaatgacaa attacaaac ctggtcaga 
      aaattcata aaagtctgg gccctattt ttaaaatgt 
      cacaaatta tgggactgt gtcctgtta tttaccaca 
      aacatgaaa acgcattca aatggtcct ggaaattat 
      atattacct cccttgtga taattacaa ttttaggtg 
      agtatattt ttttttact ttttatgaa attttttat 
      agcatgttg gtcgttttg gggcttcgt acgtgacat 
      gcaagtggc ttccttcac agccttggg cttcagagg 
      cactgctga ttttgtgat tgcttgctt cgatgtcaa 
      tgatgtgat tgttagtgc gattttttt cgtcacttc 
      cacaccttt caaatttaa gcattttgt gcaaatcgt 
      cgagaattt tgaccgaat tgacgctcg catctctcc 
      aatcttggt ggaacagat tcgaaaaag gagcaatat 
      ttttgtaaa agtcttggt aactttttt gccaacttt 
      atacgtcct tgatctgtt tatgtgggg gaaaaacaa 
      ctgggaggg attaaataa ttattttgc tttttacat 
      aatgtacag cattgttgt cgttcacga attgcagta 
      ttggcatat catgactat gatgtatgc ccgtatttt 
      ggggctcaa taaaacttt gagagatta ctttaaaaa 
      taaaacagg attctgcga acatgaaat attagtggt 
      cacccaaag ttttaattc gatcaatga ttcggtgga 
      agaaattaa taaatgttt tagttacag tacgactac 
      aattatttt cagctgtta tattcattt agtaataag 
      tccgtacca actttttgt ggtagtctc aagcactga 
      aacttctct atttaacta tgtgtattt actgtggat 
      ctctttaca cattatgag agttttaac aattgtcga 
      agtttgtca gaaatgtga aaatgagaa ccgaaaaac 
      tagaagttt agtttatca attattact ttgtaaatt 
      aaacgaaaa agtcaaaaa catggttag agtactttt 
      ctttttggt aacaactag aaaaatctt gttttctgc 
      atatgcttt gccgaaaat taacagaag acttatcat 
      ttccattct tagttctat cagtactta ctggatgat 
      tttgatgca atccacttc cagaacaat acaagtggt 
      gtaactaca acgtgtttt taaatgatt ctcatctag 
      tcgtctgta aatttctca agtacaatc agaaaatgc 
      cgcttcatg gaattaggc atttattgt agacatcga 
      atattatta ggtaccatt ctccattta ttaagtctc 
      gaatttgga aataagctt cgataataa aagtgtttt 
      cttcca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY