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Gene information
General information
TC033900 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450821.1: 1..1809
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033900
    iBeetle screen iB_08835

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033900 RA
      MKFKKPTPR PSPHADLPA PLPGRKLPV WKRKTLSTP 
      SHSSSPLSY SDTKWSPSS DPLSIPDNT PKPTRFEWP 
      EDDLPPLTL MDTSFEDIS YTSKPLLTL EEDHRASTS 
      FDDLKQIPD ANRSTPSSK KSSASLDME EMKNYVKEQ 
      KLRSGLSQD EEAVDDLDV EESGVKRKA GEQKKGIVK 
      KVRFDEQEN KTTTISIAT DGKGTGELN HEKIVTELL 
      KKYPQLVKK NKNIRLKIM AKNKAGLEQ TKGENIQIR 
      GQKLKMQND RRNPNMQES SRYD
    CDS sequence
    • >TC033900 RA
      atgaagttt aaaaagcca actcctagg ccctccccc 
      cacgcggac cttccggca cccctccct ggccgcaaa 
      ctcccagtt tggaaacgc aaaaccctc tccacaccc 
      tcccacagt tcctctcct ttatcatac tccgacaca 
      aaatggtcc ccgtcctcc gaccccctt tccatccct 
      gataacacc cccaaaccc accagattc gagtggcct 
      gaggatgat ctcccccca ctaactctc atggacaca 
      agtttcgaa gatatttcc tacaccagt aaaccgctc 
      ctgactttg gaggaagat cacagagcc agcacatca 
      tttgatgac cttaaacaa atccccgac gctaatcgt 
      tctactccc agttcgaag aaatcaagc gctagcttg 
      gacatggaa gaaatgaag aattatgtc aaagaacag 
      aaacttcgt tcggggttg agtcaggat gaggaggcc 
      gtggatgat ttggacgtg gaagagagt ggcgtgaag 
      aggaaagca ggggaacag aagaaggga attgtgaag 
      aaagtgaga tttgatgaa caggagaat aagactacg 
      acgattagt attgcgact gatgggaaa ggtacaggg 
      gaattgaac catgagaag attgtgacg gaacttttg 
      aagaaatat ccacagttg gtcaagaag aataagaat 
      atcaggttg aagattatg gcgaagaat aaagccggt 
      ttagagcaa accaaaggg gagaatata cagatacgg 
      gggcagaaa ttgaagatg cagaatgac cggcggaat 
      ccaaatatg caagaatca agtcgctac gactaa
    mRNA sequence
    • >TC033900 RA
      tttttttta aactcactc cagtaaatt tttatggac 
      ttctacatg tcttagcaa cccacaaaa gttgttttt 
      agtccacaa aaagtccat atgttcgat tttttgatg 
      tttgatttt ttcaatttt cgtcgcagt ttttgtcga 
      ttttgggta cccggaact tttgtcaag caatagctc 
      cggaactat cagagataa ccccatgaa gtttaaaaa 
      gccaactcc taggccctc cccccacgc ggaccttcc 
      ggcacccct ccctggccg caaactccc agtttggaa 
      acgcaaaac cctctccac accctccca cagttcctc 
      tcctttatc atactccga cacaaaatg gtccccgtc 
      ctccgaccc cctttccat ccctgataa cacccccaa 
      acccaccag attcgagtg gcctgagga tgatctccc 
      cccactaac tctcatgga cacaagttt cgaagatat 
      ttcctacac cagtaaacc gctcctgac tttggagga 
      agatcacag agccagcac atcatttga tgaccttaa 
      acaaatccc cgacgctaa tcgttctac tcccagttc 
      gaagaaatc aagcgctag cttggacat ggaagaaat 
      gaagaatta tgtcaaaga acagaaact tcgttcggg 
      gttgagtca ggatgagga ggccgtgga tgatttgga 
      cgtggaaga gagtggcgt gaagaggaa agcagggga 
      acagaagaa gggaattgt gaagaaagt gagatttga 
      tgaacagga gaataagac tacgacgat tagtattgc 
      gactgatgg gaaaggtac aggggaatt gaaccatga 
      gaagattgt gacggaact tttgaagaa atatccaca 
      gttggtcaa gaagaataa gaatatcag gttgaagat 
      tatggcgaa gaataaagc cggtttaga gcaaaccaa 
      aggggagaa tatacagat acgggggca gaaattgaa 
      gatgcagaa tgaccggcg gaatccaaa tatgcaaga 
      atcaagtcg ctacgacta accgttcgg aaaaatatc 
      ggcaaaaat ttagtcttg tgaaaattt atcgaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY