Status
Please wait ...
Gene information
General information
TC008610 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15945377..15956801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008610
    iBeetle screen iB_08834

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008610 RA
      MMVDHIVQG SCDTFHIPN QSIGITFYN ELKRKNHEK 
      IALIDNEEI SYGKLCKTS INLASQLIK LGLKKGDVV 
      SIVSQNNWK YLAATISGF YIGAKINFL NHDYTSGEL 
      KHFFTICPP NLIFCSKKS CNNILFLRN EGLLPEHII 
      LFDEDETEY LTFDELVKI DSDFYPVEV QPETDIAII 
      PTSSGTTGL PKCVLLTHA NLRVPLIHF GDRNFLDFK 
      EDDVTIGNL PFFHIWGNM IALTSVFYG IKLIIIPKF 
      RPEVYLKTI EDYKIETLF TVPPLLIFL AKSPLVSFY 
      DISSVKDVI CAAAVITKE LEEMVKDRL GLKAVRQLY 
      GMTEASLGI TMSPTKSEK VASVGKVLP TNKIKVCDI 
      ETQEALGPH KIGELRAKG GGLMVGYLS NKNATMEAF 
      DNEGYLRTG DLGYYDEEN FFYIVDRLK DIIKFKGFQ 
      ISPAELENL LIQHPAVKD AAVIGIPDE VAGEVAMAF 
      VVKQPDKNV TEKELVCFV NENVCAQKR LYGGVRFID 
      EIPKTSSGK IWRLKLREI V
    CDS sequence
    • >TC008610 RA
      atgatggtt gatcatatt gtacaagga tcttgtgat 
      acttttcac atcccaaat caatcaatc ggtattacc 
      ttctacaat gaattaaaa agaaaaaat catgaaaaa 
      atagcactt attgataat gaagaaata agttatgga 
      aaattatgc aaaacttct attaattta gcctcgcaa 
      ttaattaaa ttgggactg aaaaaaggc gatgtagta 
      tcgatagta tcccaaaac aactggaaa tatctcgct 
      gcaacaatt tccgggttt tacatcggc gccaaaatc 
      aatttcctc aatcatgac tatacttcc ggcgagctt 
      aaacacttt tttacgatt tgtcctcca aatctgatc 
      ttttgttcg aaaaaatcc tgtaataat atattgttt 
      ttacgaaat gaaggccta ttacctgaa catataatt 
      ctgtttgat gaggacgaa acagagtat ttgactttt 
      gatgagttg gtcaaaatt gattcggat ttttaccca 
      gtcgaagtc caaccagaa acagacatc gccattatt 
      ccaacatct tcagggacc acaggactg ccaaaatgt 
      gttctgttg actcatgcc aatcttaga gtaccgctg 
      attcatttt ggtgaccgc aattttttg gattttaaa 
      gaagacgat gtaacaatc ggaaattta ccgtttttc 
      cacatttgg ggcaacatg attgccctg actagcgtt 
      ttctacggc atcaaatta attataatt ccaaaattt 
      agacccgaa gtttattta aaaaccatc gaggattac 
      aaaatcgag acactcttc accgttcct ccactactt 
      atatttttg gccaaaagt ccgcttgtc agtttttac 
      gacatttcg tcagttaaa gacgtcatt tgtgctgct 
      gctgttatt accaaagaa ttggaagaa atggttaaa 
      gacagatta ggattaaaa gctgtccgc cagctctac 
      ggcatgact gaagcctct ctcggtatt acaatgtcg 
      ccaacaaaa agcgaaaaa gtcgccagt gtgggcaaa 
      gtattgccc accaataaa atcaaagtt tgcgatatt 
      gaaacacaa gaggctttg gggccccac aaaatcggg 
      gaattgcga gcgaaaggc ggtggttta atggtgggc 
      tacttgtct aacaaaaac gcaacaatg gaagctttc 
      gataatgaa ggttatcta agaactggc gatttgggt 
      tattacgac gaagaaaat tttttttac attgttgac 
      aggttaaaa gacatcatt aagtttaaa ggatttcaa 
      atatctcct gctgaatta gaaaacttg ttgattcag 
      catcctgca gtgaaagac gcagcagtg attggaatt 
      cctgatgaa gtagcaggg gaagtagcg atggctttt 
      gtggtcaaa caaccggat aaaaatgtg acggagaaa 
      gaacttgtt tgttttgtt aatgagaat gtttgtgca 
      cagaaacgg ctctatgga ggtgtgagg ttcatcgat 
      gagattccc aaaacgtcc agtggcaag atttggaga 
      ctcaaattg agagaaatt gtttga
    mRNA sequence
    • >TC008610 RA
      ttttccgta acacctccc cccttaaga cattttgat 
      atcaggaaa gatgtcaaa atacgcaac gacttaaca 
      ttcacacga tcacatgcg tttattggc ccttgaata 
      ccgcactca tgcactatt actgattga aggcatttg 
      aaatcttat ttctttttc attggagtc acaacctgt 
      cgaatgaag gaagggacg ggcgccggg tgactgttg 
      atcctacgt tgcaataat tgaaaacct gaggacagc 
      atccgctgt gaggacggt gatcatagt cattgccct 
      gagtgttca aacaattgc gaattttcc ttttgatgc 
      cgcatttac aaagaagcc aaactatta tgacagata 
      ttgagaaga tggtaacat aggtgatcg ttccccagt 
      gtttggatg tgaatggtt ccctgatgc tgcggtcga 
      attttttcg tcaatttct cggactttc tgatctgca 
      gtattcaac tcgtcgaga tttaaatga ttaatttga 
      agaggttga agtttcggt aatttctct ttttcggat 
      acaattgtc ttctatgct agcgtaaat gcgtgtcgt 
      tccaacaaa agcactaca ttttgagtt taaagttaa 
      aagactgtt ttcttcgat tatttcatt tttcgaatt 
      ctctcctgg acaaatggt tgttactgt agtcggatg 
      cgagatagt taggagcca cttgttatt ggctagttt 
      ttggatatt ataatcact aattgatgt gattatacc 
      gtcacagtg ctgagtcca ttatgagtg ttatacttc 
      acatgaagc gcttggtga caacatttt cggaaaacg 
      gtcgtagca tgttttatc tggttttgg acaacttcg 
      gtgcaatca tccttgtcc agcgtaatg tattgtttg 
      ctttctctt gatacagca acatatttg tggagaggg 
      ataattgtg taaaaaaga ttggttttg ggattttta 
      gttatagct ggagtaggt atgataata gtcgtactc 
      ggcggattt gacaattgg atcgtgaag gataaaaac 
      gattttgtt aaatgtttg aaaagcttc gattttaat 
      caaagttaa aatttttta taaattatt gtcttttgg 
      aatgaaagg tagatttcc atgagttaa tttttgagg 
      tatttttgt cagttgttt taacaatag ctcaggttc 
      gataattga tgcatgcgg aatctgtaa gcgagcgaa 
      agttacact agtgataag gtcatttaa ttcatcctg 
      aaggatcga tcataagag attccaagt cttgagcaa 
      caatttgaa ataatttaa gctgaaaat catgaaaat 
      agatatttt gagaaaatt gatttagtc tctgtgaga 
      taatttcta aatctttat ttagcgtaa taccgtctt 
      taatgcttt aatttagaa actgtttca ttgaatttt 
      tttatggta ctggaaata attttaatt tggtttttc 
      ataaggttt ttaacttct aattcgccc ttttcaagt 
      cgttcaatg caatcgtta taatactga ccgtcatct 
      caatctaaa ctgcccgca gttagtttc caaacaaac 
      ccgaggcca ttaatagtt cctctttta aatttattg 
      gcaatcgat gatggtaat ttaatttcc ctatttttg 
      tgaggataa ggcattaag aaaattcag ttgggattg 
      gattgacac tctgaaggc aggatcttg ttttggtcg 
      tcgtatcat attttcaat ttgtttttg tgcattttt 
      gttttcatg aaataattc ctctaacat gatggttga 
      tcatattgt acaaggatc ttgtgatac ttttcacat 
      cccaaatca atcaatcgg tattacctt ctacaatga 
      attaaaaag aaaaaatca tgaaaaaat agcacttat 
      tgataatga agaaataag ttatggaaa attatgcaa 
      aacttctat taatttagc ctcgcaatt aattaaatt 
      gggactgaa aaaaggcga tgtagtatc gatagtatc 
      ccaaaacaa ctggaaata tctcgctgc aacaatttc 
      cgggtttta catcggcgc caaaatcaa tttcctcaa 
      tcatgacta tacttccgg cgagcttaa acacttttt 
      tacgatttg tcctccaaa tctgatctt ttgttcgaa 
      aaaatcctg taataatat attgttttt acgaaatga 
      aggcctatt acctgaaca tataattct gtttgatga 
      ggacgaaac agagtattt gacttttga tgagttggt 
      caaaattga ttcggattt ttacccagt cgaagtcca 
      accagaaac agacatcgc cattattcc aacatcttc 
      agggaccac aggactgcc aaaatgtgt tctgttgac 
      tcatgccaa tcttagagt accgctgat tcattttgg 
      tgaccgcaa ttttttgga ttttaaaga agacgatgt 
      aacaatcgg aaatttacc gtttttcca catttgggg 
      caacatgat tgccctgac tagcgtttt ctacggcat 
      caaattaat tataattcc aaaatttag acccgaagt 
      ttatttaaa aaccatcga ggattacaa aatcgagac 
      actcttcac cgttcctcc actacttat atttttggc 
      caaaagtcc gcttgtcag tttttacga catttcgtc 
      agttaaaga cgtcatttg tgctgctgc tgttattac 
      caaagaatt ggaagaaat ggttaaaga cagattagg 
      attaaaagc tgtccgcca gctctacgg catgactga 
      agcctctct cggtattac aatgtcgcc aacaaaaag 
      cgaaaaagt cgccagtgt gggcaaagt attgcccac 
      caataaaat caaagtttg cgatattga aacacaaga 
      ggctttggg gccccacaa aatcgggga attgcgagc 
      gaaaggcgg tggtttaat ggtgggcta cttgtctaa 
      caaaaacgc aacaatgga agctttcga taatgaagg 
      ttatctaag aactggcga tttgggtta ttacgacga 
      agaaaattt tttttacat tgttgacag gttaaaaga 
      catcattaa gtttaaagg atttcaaat atctcctgc 
      tgaattaga aaacttgtt gattcagca tcctgcagt 
      gaaagacgc agcagtgat tggaattcc tgatgaagt 
      agcagggga agtagcgat ggcttttgt ggtcaaaca 
      accggataa aaatgtgac ggagaaaga acttgtttg 
      ttttgttaa tgagaatgt ttgtgcaca gaaacggct 
      ctatggagg tgtgaggtt catcgatga gattcccaa 
      aacgtccag tggcaagat ttggagact caaattgag 
      agaaattgt ttgatgtag agaaaaggg cttatctat 
      tagcttatc cgtggggtt gatcttgaa tataataaa 
      tattgtttt aattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY