Status
Please wait ...
Gene information
General information
TC008734 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15232256..15248535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008734
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08829

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008734 RA
      MIIRWELSL GLLLVLLSI AIARKESSK CPMLTECPL 
      RAQHCSEDD DCSPDSICC KSPCGKVCT KQLFTGCQT 
      LRMAASRRA KALGVEARS VRMPRCNKS GGFEPIQCD 
      NEIVSSCWC VDEAGFELP GTRAPAAAL VNCTVPKPC 
      ADHTCRMFC PHGFALAPD GCPLCRCRD PCEGIKCPN 
      ALECHLEEL ACADPPCPP VPTCKRGRS LENICPVGD 
      PLRISDTVR PFLCGNDPG KPTCPPMYQ CLVQKGNDY 
      GVCCPASLK IQKTGTCPE KIDAGDECG QMCSHDLEC 
      PSVQKCCQT QQCGASCTH PKNVTECLH QRALSEILA 
      VSERAGRGY VPQCSEDGQ FEPKQCSRN SLVCWCVDR 
      MGRKIRGSM GPAGNTNCS ILDARIQSS ARSLDKSGK 
      QCESLECAA VCEYGFKLD EDGCPTCKC DDPCDGYMC 
      PEDEECINV KESTCTDFL CPTLPVCRP KTIYANPCD 
      QGAPLTDDI SGAPVACAL RSEDGTICP SEYECTAVP 
      GSTQAVCCP REMIEEENV TENRPQTMC EYLHDFSES 
      MEGTREGMT LALPSPSCD SDGNYISTQ CHKGECWCV 
      DNFGTEIPR TRGTTQNCT ELRQSLDCL DLTCRMGCE 
      YGFVLSEET GCPTCQCRD PCSGIKCEE NSQCQLVEV 
      SCKDHYCPP VPACLPKKQ GQCPYLVPA TSTSCDFEC 
      NSDMACNGT MRCCSNGCG TQCVEPLLL TACQHQNAL 
      SQHQAHESG VPAGRVYIP QCTPEGAYE PKQCNPGTN 
      ECWCVDWRG FEISKTRTN SQLSCETIQ QSDCPLYKC 
      INDCEHGFE MDANGCRTC DCIDPCSRI SCRGEGETC 
      RLVPVECTN EPCPPIPMC LPKKDNPCQ NGDPLKLGN 
      SEEIVTCGP DYETCPSSH KCQLSPVGE YAVCCPKPR 
      DVCFEALDK GDCGDDFSR NLTRWYFNS RTNKCEMFV 
      YSGCNGNHN NFHSEEMCN LVCPVLSQC ERLREKNQR 
      AAERYHKPT FMPRCEANT GNWETVQCL EHVGVCWCV 
      TPQGEPLKG TLTRGAQPL CNFRQARNR AEDRADTSE 
      ADLVLEELM MQIGSFDDE EEPSDLDEP LEVPSNTRC 
      EALGGQCDT TGKFLPTQC EEETCWCVD EAGNQLLHT 
      NTFKKGEIT CKQVPVESV EVTLGFRGE YDDISSIPV 
      INQVTKIIQ SLEGILSGG FKTEISDDV LYVKFSLIG 
      SNKVDVAYR LEQLVMQQR LPGLTADIT RSRVTHKLL 
      QEPALGDHL ALEHREIVS QSPVSVVAP YHTALIVIA 
      AASAFVICV LLLLVILYR RKMNSLSST KVGDDAGFL 
      SNNRPIYIE LPNEKYNTI SPDVKTENS
    CDS sequence
    • >TC008734 RA
      atgattatt cgttgggaa ttgagttta ggtcttctc 
      cttgtgctt ctctcgata gcaatcgcc aggaaagag 
      tcgtccaaa tgccccatg ttgaccgaa tgccctttg 
      cgagcccag cactgctcc gaagacgac gactgttcg 
      cccgatagc atttgctgc aagagtcct tgcggcaaa 
      gtctgcacc aagcagctt ttcacagga tgtcaaacg 
      ttgcgaatg gcggcgtca agacgggcc aaagctcta 
      ggtgtcgag gccaggagt gtgcgaatg ccacgatgt 
      aataaatct gggggtttt gaaccgatc cagtgcgat 
      aatgagatt gtgagttcc tgctggtgc gtggacgaa 
      gccggcttc gagttgccg gggactcga gcccctgca 
      gcggctctg gttaattgc acagtgccg aagccgtgc 
      gcggaccac acctgccgc atgttctgc ccccacggc 
      ttcgccctc gcccctgac gggtgcccc ctttgtcgt 
      tgccgtgac ccttgcgaa ggcatcaaa tgccccaac 
      gctctcgaa tgccacctc gaagagctc gcgtgtgcc 
      gaccctccg tgcccccct gtccccaca tgcaagagg 
      ggacgaagt ttggagaat atctgtcct gtaggagac 
      cctttgcgc ataagcgat accgtcaga ccgtttcta 
      tgcggaaat gatccgggg aaacccact tgtccccct 
      atgtatcag tgtttagtc cagaaaggg aacgactat 
      ggagtttgc tgtccggcg tctttgaaa atacagaaa 
      accggcact tgtcctgag aagattgat gcgggggac 
      gagtgtgga cagatgtgc agtcacgat ttggaatgt 
      ccttcggtt caaaagtgt tgccaaaca caacaatgc 
      ggggcttcg tgtacccat cccaagaat gttacagag 
      tgtttacat cagcgcgcc ttgtctgaa attttggcc 
      gtgagtgaa cgagcaggc cgaggttac gtgccccag 
      tgctctgaa gatggccaa tttgagccg aaacagtgc 
      agtcgcaac agcttagtt tgttggtgt gtcgatcgc 
      atgggacgc aaaatccgt ggttctatg ggacctgcc 
      ggaaatacc aactgctca atcttggat gcgcgtatt 
      caatcatct gctcgaagc ctcgacaaa agcggcaaa 
      caatgcgaa tcgttagaa tgtgccgct gtttgcgag 
      tacggtttc aagctggac gaagatgga tgtccaaca 
      tgcaaatgt gacgaccct tgtgatggt tacatgtgt 
      cccgaagac gaggaatgc ataaatgtg aaagaatca 
      acgtgtacg gattttcta tgcccgact ttaccagtt 
      tgccgacca aaaacaatt tatgcgaat ccgtgtgat 
      caaggggct cctttgact gacgatata agtggagca 
      ccggtcgcc tgtgccctt cgttccgaa gatggtact 
      atttgtcct tcagagtac gaatgcacg gcagttccg 
      ggttcaacg caagccgtt tgttgtcca cgagaaatg 
      atagaagag gaaaatgtc accgaaaat cgcccacaa 
      acaatgtgt gaatacttg cacgacttt tccgaaagt 
      atggaagga actcgcgag ggtatgacc ctggccctt 
      ccaagccct tcgtgtgat tctgatggt aattacatc 
      tcaacccaa tgccacaaa ggtgaatgt tggtgtgtc 
      gacaatttc ggtactgaa atcccccga actcgaggt 
      accacccaa aactgtacc gaactgaga cagtcactg 
      gattgtttg gacttgact tgtcgcatg ggttgcgaa 
      tatgggttc gtcttatct gaagaaaca ggttgtccc 
      acttgccaa tgccgcgac ccatgcagt ggaatcaaa 
      tgcgaggag aactcacag tgccaattg gtcgaagtg 
      agttgcaag gaccattac tgccctcct gtcccagcc 
      tgtctccct aaaaaacag ggccaatgc ccatatttg 
      gtccccgcc acgtccacg tcctgcgat tttgagtgc 
      aattcggat atggcttgc aatggtacc atgcggtgt 
      tgttccaat ggttgtggc acacagtgc gtggaacct 
      ctccttctg actgcctgc cagcaccaa aacgcgctc 
      tcccagcac caagcccac gaatccgga gtaccagca 
      ggccgggtt tacatccca caatgcacc cctgaaggt 
      gcctacgaa cccaagcag tgcaatcct ggtaccaac 
      gagtgttgg tgcgtggat tggcgaggt tttgaaatt 
      tccaaaacc aggacaaac tcccagctt tcatgcgaa 
      acaatccaa cagtcggat tgtcctctt tacaaatgt 
      atcaacgat tgtgagcac ggcttcgaa atggatgcc 
      aatggatgt cgtacctgt gattgcata gacccatgc 
      tcgcgcatt agctgtcgt ggggaaggc gagacatgt 
      cgcttagtc ccagtggaa tgtaccaac gaaccttgc 
      ccacccatt cccatgtgt ctcccgaaa aaggacaac 
      ccatgccaa aacggcgac cccttgaaa ctgggcaat 
      agcgaagaa atcgtcact tgtggcccc gactacgaa 
      acgtgtcct tcatcacac aaatgccaa ttgagtcct 
      gtgggagaa tatgcagtc tgttgccct aaacctaga 
      gatgtgtgt ttcgaagct ttggataag ggcgattgt 
      ggggatgat ttctcgcgg aatttgacg cgttggtac 
      tttaattcc cggacgaac aaatgcgag atgtttgtg 
      tattcgggt tgtaacggg aatcataac aacttccat 
      tcggaggaa atgtgcaat ttggtttgt cccgtcttg 
      agccagtgt gaaagatta agagagaag aatcaaagg 
      gcggcggag aggtaccat aaacccact tttatgccg 
      cgctgtgaa gctaacact gggaactgg gagacggtg 
      caatgtttg gaacatgtg ggggtttgc tggtgtgtt 
      accccacaa ggagagcct ctgaaaggg acgctgaca 
      aggggggcc cagcccttg tgtaacttc aggcaagca 
      aggaatcgc gcggaggat cgagctgac accagcgag 
      gccgattta gttcttgag gaattaatg atgcaaatc 
      ggttcgttc gacgacgag gaagaaccg agcgatttg 
      gacgagccc ttggaagtc ccctcaaac acccgatgc 
      gaagctcta ggtggccaa tgcgacacc accggtaaa 
      ttcctccca actcaatgc gaagaagaa acgtgttgg 
      tgcgtcgac gaagccggc aaccaactc ctacacaca 
      aacaccttc aaaaaagga gaaattacg tgcaaacaa 
      gttccagtc gagagtgtt gaagtaact ctaggtttc 
      cgtggcgaa tacgacgat atttcttcg atcccggtc 
      attaaccaa gtcacaaaa attatacaa agtcttgag 
      gggattctc agtggcggc ttcaaaacg gaaataagc 
      gacgatgtt ctctacgtt aaattttct ctaattggg 
      agcaacaaa gtcgacgtt gcttatcga ctggaacag 
      cttgtgatg cagcagaga cttccgggt ctaactgct 
      gatatcacc aggtcaagg gttacgcat aaactgctc 
      caggagccg gcacttggt gatcatttg gcgctagaa 
      caccgggaa attgtgtcg cagtcgccg gtttcggtg 
      gtggctcct tatcacacg gctctgatt gtcatagcg 
      gccgcctca gcatttgtc atttgtgtt ctactcctt 
      ttggttatt ttgtacaga aggaaaatg aatagtctt 
      agtagcacc aaggttggg gatgatgct gggtttttg 
      tccaacaat aggccaatt tatattgaa ctccccaac 
      gaaaaatac aacacgata tcaccagac gtgaaaact 
      gaaaattcc tag
    mRNA sequence
    • >TC008734 RA
      ctgctaaga agcccaaga cagcagtcc gctaaaatc 
      caccgacag ctccggtag tgttattcg tttgtgtcg 
      cgtgttatt gttgtattc tatgacgat ctgaacgga 
      ttttccaga ctacaccca acatttttc accaaacat 
      tggtctagc tatgattat tcgttggga attgagttt 
      aggtcttct ccttgtgct tctctcgat agcaatcgc 
      caggaaaga gtcgtccaa atgccccat gttgaccga 
      atgcccttt gcgagccca gcactgctc cgaagacga 
      cgactgttc gcccgatag catttgctg caagagtcc 
      ttgcggcaa agtctgcac caagcagct tttcacagg 
      atgtcaaac gttgcgaat ggcggcgtc aagacgggc 
      caaagctct aggtgtcga ggccaggag tgtgcgaat 
      gccacgatg taataaatc tgggggttt tgaaccgat 
      ccagtgcga taatgagat tgtgagttc ctgctggtg 
      cgtggacga agccggctt cgagttgcc ggggactcg 
      agcccctgc agcggctct ggttaattg cacagtgcc 
      gaagccgtg cgcggacca cacctgccg catgttctg 
      cccccacgg cttcgccct cgcccctga cgggtgccc 
      cctttgtcg ttgccgtga cccttgcga aggcatcaa 
      atgccccaa cgctctcga atgccacct cgaagagct 
      cgcgtgtgc cgaccctcc gtgcccccc tgtccccac 
      atgcaagag gggacgaag tttggagaa tatctgtcc 
      tgtaggaga ccctttgcg cataagcga taccgtcag 
      accgtttct atgcggaaa tgatccggg gaaacccac 
      ttgtccccc tatgtatca gtgtttagt ccagaaagg 
      gaacgacta tggagtttg ctgtccggc gtctttgaa 
      aatacagaa aaccggcac ttgtcctga gaagattga 
      tgcggggga cgagtgtgg acagatgtg cagtcacga 
      tttggaatg tccttcggt tcaaaagtg ttgccaaac 
      acaacaatg cggggcttc gtgtaccca tcccaagaa 
      tgttacaga gtgtttaca tcagcgcgc cttgtctga 
      aattttggc cgtgagtga acgagcagg ccgaggtta 
      cgtgcccca gtgctctga agatggcca atttgagcc 
      gaaacagtg cagtcgcaa cagcttagt ttgttggtg 
      tgtcgatcg catgggacg caaaatccg tggttctat 
      gggacctgc cggaaatac caactgctc aatcttgga 
      tgcgcgtat tcaatcatc tgctcgaag cctcgacaa 
      aagcggcaa acaatgcga atcgttaga atgtgccgc 
      tgtttgcga gtacggttt caagctgga cgaagatgg 
      atgtccaac atgcaaatg tgacgaccc ttgtgatgg 
      ttacatgtg tcccgaaga cgaggaatg cataaatgt 
      gaaagaatc aacgtgtac ggattttct atgcccgac 
      tttaccagt ttgccgacc aaaaacaat ttatgcgaa 
      tccgtgtga tcaaggggc tcctttgac tgacgatat 
      aagtggagc accggtcgc ctgtgccct tcgttccga 
      agatggtac tatttgtcc ttcagagta cgaatgcac 
      ggcagttcc gggttcaac gcaagccgt ttgttgtcc 
      acgagaaat gatagaaga ggaaaatgt caccgaaaa 
      tcgcccaca aacaatgtg tgaatactt gcacgactt 
      ttccgaaag tatggaagg aactcgcga gggtatgac 
      cctggccct tccaagccc ttcgtgtga ttctgatgg 
      taattacat ctcaaccca atgccacaa aggtgaatg 
      ttggtgtgt cgacaattt cggtactga aatcccccg 
      aactcgagg taccaccca aaactgtac cgaactgag 
      acagtcact ggattgttt ggacttgac ttgtcgcat 
      gggttgcga atatgggtt cgtcttatc tgaagaaac 
      aggttgtcc cacttgcca atgccgcga cccatgcag 
      tggaatcaa atgcgagga gaactcaca gtgccaatt 
      ggtcgaagt gagttgcaa ggaccatta ctgccctcc 
      tgtcccagc ctgtctccc taaaaaaca gggccaatg 
      cccatattt ggtccccgc cacgtccac gtcctgcga 
      ttttgagtg caattcgga tatggcttg caatggtac 
      catgcggtg ttgttccaa tggttgtgg cacacagtg 
      cgtggaacc tctccttct gactgcctg ccagcacca 
      aaacgcgct ctcccagca ccaagccca cgaatccgg 
      agtaccagc aggccgggt ttacatccc acaatgcac 
      ccctgaagg tgcctacga acccaagca gtgcaatcc 
      tggtaccaa cgagtgttg gtgcgtgga ttggcgagg 
      ttttgaaat ttccaaaac caggacaaa ctcccagct 
      ttcatgcga aacaatcca acagtcgga ttgtcctct 
      ttacaaatg tatcaacga ttgtgagca cggcttcga 
      aatggatgc caatggatg tcgtacctg tgattgcat 
      agacccatg ctcgcgcat tagctgtcg tggggaagg 
      cgagacatg tcgcttagt cccagtgga atgtaccaa 
      cgaaccttg cccacccat tcccatgtg tctcccgaa 
      aaaggacaa cccatgcca aaacggcga ccccttgaa 
      actgggcaa tagcgaaga aatcgtcac ttgtggccc 
      cgactacga aacgtgtcc ttcatcaca caaatgcca 
      attgagtcc tgtgggaga atatgcagt ctgttgccc 
      taaacctag agatgtgtg tttcgaagc tttggataa 
      gggcgattg tggggatga tttctcgcg gaatttgac 
      gcgttggta ctttaattc ccggacgaa caaatgcga 
      gatgtttgt gtattcggg ttgtaacgg gaatcataa 
      caacttcca ttcggagga aatgtgcaa tttggtttg 
      tcccgtctt gagccagtg tgaaagatt aagagagaa 
      gaatcaaag ggcggcgga gaggtacca taaacccac 
      ttttatgcc gcgctgtga agctaacac tgggaactg 
      ggagacggt gcaatgttt ggaacatgt gggggtttg 
      ctggtgtgt taccccaca aggagagcc tctgaaagg 
      gacgctgac aaggggggc ccagccctt gtgtaactt 
      caggcaagc aaggaatcg cgcggagga tcgagctga 
      caccagcga ggccgattt agttcttga ggaattaat 
      gatgcaaat cggttcgtt cgacgacga ggaagaacc 
      gagcgattt ggacgagcc cttggaagt cccctcaaa 
      cacccgatg cgaagctct aggtggcca atgcgacac 
      caccggtaa attcctccc aactcaatg cgaagaaga 
      aacgtgttg gtgcgtcga cgaagccgg caaccaact 
      cctacacac aaacacctt caaaaaagg agaaattac 
      gtgcaaaca agttccagt cgagagtgt tgaagtaac 
      tctaggttt ccgtggcga atacgacga tatttcttc 
      gatcccggt cattaacca agtcacaaa aattataca 
      aagtcttga ggggattct cagtggcgg cttcaaaac 
      ggaaataag cgacgatgt tctctacgt taaattttc 
      tctaattgg gagcaacaa agtcgacgt tgcttatcg 
      actggaaca gcttgtgat gcagcagag acttccggg 
      tctaactgc tgatatcac caggtcaag ggttacgca 
      taaactgct ccaggagcc ggcacttgg tgatcattt 
      ggcgctaga acaccggga aattgtgtc gcagtcgcc 
      ggtttcggt ggtggctcc ttatcacac ggctctgat 
      tgtcatagc ggccgcctc agcatttgt catttgtgt 
      tctactcct tttggttat tttgtacag aaggaaaat 
      gaatagtct tagtagcac caaggttgg ggatgatgc 
      tgggttttt gtccaacaa taggccaat ttatattga 
      actccccaa cgaaaaata caacacgat atcaccaga 
      cgtgaaaac tgaaaattc ctagttttg ctagattgc 
      catttgaat gaaactttg tgattcgtt tactggaag 
      actttatta ggattacgg tgttttttg ggtttgttc 
      atgtggcac taaataaaa caaatatct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY