Status
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Gene information
General information
TC010114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14558348..14571160
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010114
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08823

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    vitellarium potentiallysize shortened - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010114 RA
      MSQARNKYQ NNNPHGLHG RPDFTKHKK KFRFHQRPY 
      DRFHYYNGY QPRWNTGHW RECFVPRQA PRKQPSRSP 
      SPFSGTEEY TQRKIQETS DMIKKQLMM PPEADEIVN 
      KEPPPEAPT QSEATQNDK MSSKSEKSV AKPKKVAHY 
      NVEEIHNKI ITHISKLSH GKKMNLICS TSSGYDIAI 
      QQIQKQKRL ELSKVLRDM CNSPAVENS EVINAIIPD 
      IGIKIGDLP LEVIQELSS TLDLNFDDS SFWESGDDT 
      QNVKSEVCE NVTEKQMNM TEEVLNIKE EVLNIKEEV 
      NQDQSEFDD STLSIFNIK QEFISDNGS LDETKLFEV 
      TEDPYNELQ APSTENKSQ VFAAQPVWC ESQTDFVEF 
      HSIACQTDP NSCKFEVRE NVENLEDAV KVMLEIDNE 
      IARLTKLRQ TIFTKLQAK NMLKNQVNK GKSSQKKSP 
      ANKLKNKLI KRSLKLKSE AAPKRKKKS NVQPQAMKV 
      AIDCVYEDP NLQDPSTKQ VTDCVYEKP LEFSVSSEK 
      ILVLKLIDD VLLAASESG RIFYINTQT GATEATLQV 
      SQTPITCLC HIKTPSNES HLLVGSFEP WLRVYHYTT 
      RLLITISNI FECVQCLEY AWMCIFIGT TNGSLKRYS 
      PKQRLIEFE DRISEEAVL VLKATKEGA RRVLLVGSR 
      NAPVCVRDA MSGLRLRTL GDCVTPTVY SLLLENNMV 
      YCGTMSHDI LVFGFHNGF LVHKHEATN SKGICCMKV 
      VKNLLYASC YNGKIYVYN VKSHKYVGM IEGPGGVIL 
      SMEVFKNML IVGTMSHNF KSLLIPDYI LENGKA
    CDS sequence
    • >TC010114 RA
      atgtctcaa gccagaaat aagtatcaa aacaacaac 
      ccccatggc ttgcacggc agaccagac ttcactaaa 
      cacaagaaa aagtttcgc tttcatcag aggccctat 
      gatcggttt cattattac aatgggtat caaccacgc 
      tggaacacc ggacattgg agggaatgc tttgttcca 
      agacaagcc ccccgcaag caaccttcg cgatcacca 
      tcaccgttc tcaggcaca gaggaatac acccagcga 
      aagatacaa gaaacgagc gatatgatt aaaaaacag 
      ctgatgatg ccgccagag gctgacgaa attgttaac 
      aaggagcca cctcctgag gccccaact caaagcgaa 
      gccactcaa aacgacaaa atgtccagt aagagcgaa 
      aaaagcgtc gcaaagccg aagaaagtc gcgcattac 
      aacgttgaa gaaatccac aacaaaatc ataacccac 
      atcagcaaa ttgagccat ggaaagaaa atgaatctg 
      atctgttcc acctcctct gggtatgac attgccatc 
      caacagatc cagaagcaa aaacgatta gagttatca 
      aaggttttg cgagatatg tgcaacagc ccagcggtg 
      gaaaatagt gaggttata aacgcgatt attcccgat 
      attggcata aaaatcgga gacttaccc ttagaggtg 
      atccaggag ctcagcagc acgttagac ttgaatttt 
      gacgactct tcattttgg gaaagtggc gatgacact 
      caaaacgtc aaatctgaa gtttgtgaa aacgtcact 
      gaaaaacaa atgaacatg acagaagaa gttttgaac 
      atcaaggaa gaagttttg aacatcaag gaagaagtt 
      aatcaagat caaagtgaa tttgatgac tcaacttta 
      tcaattttt aatatcaaa caagagttt atttctgat 
      aatggctca ttggacgag acaaaattg tttgaagtt 
      actgaagat ccgtacaat gagctgcaa gcaccatcc 
      accgaaaat aaatctcaa gtgtttgcg gcacagccc 
      gtttggtgc gaatcacag actgatttt gtagagttc 
      cattccatt gcatgtcaa accgaccca aatagctgt 
      aagtttgaa gttcgagag aatgtagag aacttggaa 
      gacgccgtg aaagtgatg cttgaaatt gataatgaa 
      atagcacga ctgacgaag ctaagacaa acaattttt 
      actaagcta caagctaaa aacatgttg aaaaatcaa 
      gtaaataag ggcaaaagt agccagaaa aagtctccc 
      gccaataag ttgaaaaat aaactaatt aagcgaagt 
      ttaaaactt aagtcagaa gctgctcca aagcgtaaa 
      aaaaagtcg aatgtacaa cctcaagcc atgaaggtg 
      gcgatcgac tgtgtttac gaggacccc aacttacaa 
      gacccaagc acgaagcaa gtgactgat tgtgtgtat 
      gagaaaccg ctggagttt agcgtttct tcagagaaa 
      attttggtc ctaaagtta attgatgat gtccttctg 
      gccgcctcc gaaagcggc cgaattttc tacataaat 
      actcaaact ggtgcaacc gaagccact ctccaagtg 
      tctcaaacc ccgatcacg tgtctatgt cacataaaa 
      acgccctca aatgaatct cacttattg gtgggttcg 
      tttgagcct tggctccgc gtgtatcac tacaccacc 
      cgtttactg atcaccatt agcaacatt tttgaatgc 
      gtccaatgc ttggaatac gcgtggatg tgcattttc 
      attggcaca accaacggc agcctgaag cgttattct 
      ccaaagcaa agactgata gaatttgag gaccgcatc 
      tcagaagag gctgtgctt gtgctgaag gcaacgaaa 
      gagggcgct cgccgcgtt ctgttggtg ggttcccgc 
      aacgctccg gtgtgtgtt agggacgcg atgtcgggg 
      ctgcgtttg agaactttg ggggattgc gtgacacca 
      actgtatat agtttactg ctggagaat aatatggtc 
      tattgtggg acgatgtcg cacgatatt ttagttttt 
      gggtttcat aatggattt ttggtccac aaacacgaa 
      gctaccaat tccaaaggg atttgttgt atgaaagtt 
      gttaaaaat ttgctttac gccagttgc tacaatgga 
      aagatttat gtttataat gttaaaagc cataagtat 
      gtcgggatg attgaaggg ccaggaggt gtaatactc 
      tcaatggag gtttttaaa aatatgctg attgtaggg 
      acgatgtcg cataatttc aagtcgttg ttaattcct 
      gattacatt ctagaaaat gggaaggcc tga
    mRNA sequence
    • >TC010114 RA
      tctgtcaaa tgtcaattg accaggtta taaacaaat 
      cacacgata attttcaaa atattcaga aaatcacaa 
      acattgtta atgattttt cggcgaaaa caatgtctc 
      aagccagaa ataagtatc aaaacaaca acccccatg 
      gcttgcacg gcagaccag acttcacta aacacaaga 
      aaaagtttc gctttcatc agaggccct atgatcggt 
      ttcattatt acaatgggt atcaaccac gctggaaca 
      ccggacatt ggagggaat gctttgttc caagacaag 
      ccccccgca agcaacctt cgcgatcac catcaccgt 
      tctcaggca cagaggaat acacccagc gaaagatac 
      aagaaacga gcgatatga ttaaaaaac agctgatga 
      tgccgccag aggctgacg aaattgtta acaaggagc 
      cacctcctg aggccccaa ctcaaagcg aagccactc 
      aaaacgaca aaatgtcca gtaagagcg aaaaaagcg 
      tcgcaaagc cgaagaaag tcgcgcatt acaacgttg 
      aagaaatcc acaacaaaa tcataaccc acatcagca 
      aattgagcc atggaaaga aaatgaatc tgatctgtt 
      ccacctcct ctgggtatg acattgcca tccaacaga 
      tccagaagc aaaaacgat tagagttat caaaggttt 
      tgcgagata tgtgcaaca gcccagcgg tggaaaata 
      gtgaggtta taaacgcga ttattcccg atattggca 
      taaaaatcg gagacttac ccttagagg tgatccagg 
      agctcagca gcacgttag acttgaatt ttgacgact 
      cttcatttt gggaaagtg gcgatgaca ctcaaaacg 
      tcaaatctg aagtttgtg aaaacgtca ctgaaaaac 
      aaatgaaca tgacagaag aagttttga acatcaagg 
      aagaagttt tgaacatca aggaagaag ttaatcaag 
      atcaaagtg aatttgatg actcaactt tatcaattt 
      ttaatatca aacaagagt ttatttctg ataatggct 
      cattggacg agacaaaat tgtttgaag ttactgaag 
      atccgtaca atgagctgc aagcaccat ccaccgaaa 
      ataaatctc aagtgtttg cggcacagc ccgtttggt 
      gcgaatcac agactgatt ttgtagagt tccattcca 
      ttgcatgtc aaaccgacc caaatagct gtaagtttg 
      aagttcgag agaatgtag agaacttgg aagacgccg 
      tgaaagtga tgcttgaaa ttgataatg aaatagcac 
      gactgacga agctaagac aaacaattt ttactaagc 
      tacaagcta aaaacatgt tgaaaaatc aagtaaata 
      agggcaaaa gtagccaga aaaagtctc ccgccaata 
      agttgaaaa ataaactaa ttaagcgaa gtttaaaac 
      ttaagtcag aagctgctc caaagcgta aaaaaaagt 
      cgaatgtac aacctcaag ccatgaagg tggcgatcg 
      actgtgttt acgaggacc ccaacttac aagacccaa 
      gcacgaagc aagtgactg attgtgtgt atgagaaac 
      cgctggagt ttagcgttt cttcagaga aaattttgg 
      tcctaaagt taattgatg atgtccttc tggccgcct 
      ccgaaagcg gccgaattt tctacataa atactcaaa 
      ctggtgcaa ccgaagcca ctctccaag tgtctcaaa 
      ccccgatca cgtgtctat gtcacataa aaacgccct 
      caaatgaat ctcacttat tggtgggtt cgtttgagc 
      cttggctcc gcgtgtatc actacacca cccgtttac 
      tgatcacca ttagcaaca tttttgaat gcgtccaat 
      gcttggaat acgcgtgga tgtgcattt tcattggca 
      caaccaacg gcagcctga agcgttatt ctccaaagc 
      aaagactga tagaatttg aggaccgca tctcagaag 
      aggctgtgc ttgtgctga aggcaacga aagagggcg 
      ctcgccgcg ttctgttgg tgggttccc gcaacgctc 
      cggtgtgtg ttagggacg cgatgtcgg ggctgcgtt 
      tgagaactt tgggggatt gcgtgacac caactgtat 
      atagtttac tgctggaga ataatatgg tctattgtg 
      ggacgatgt cgcacgata ttttagttt ttgggtttc 
      ataatggat ttttggtcc acaaacacg aagctacca 
      attccaaag ggatttgtt gtatgaaag ttgttaaaa 
      atttgcttt acgccagtt gctacaatg gaaagattt 
      atgtttata atgttaaaa gccataagt atgtcggga 
      tgattgaag ggccaggag gtgtaatac tctcaatgg 
      aggttttta aaaatatgc tgattgtag ggacgatgt 
      cgcataatt tcaagtcgt tgttaattc ctgattaca 
      ttctagaaa atgggaagg cctgatttt accatttat 
      gattatgag tctcgagaa gcgggcatt gattttcta 
      gtcggttta gtttttaaa aatacattt atgtacgaa 
      ta
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