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Gene information
General information
TC010127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14306768..14329216
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010127
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08820

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010127 RA
      MAVIVHPLN IYMCLFFVV SAEMILTPS SLICQDMVK 
      LSRDRRAYE AHRSRDNVV KPSSGSSPS RGSVFATAN 
      STSVAAQIG GTAKLPCIV RKFNNGVVS WIRKDITPP 
      TILTVGLGP YIADDRFMV EHARHLQNW DLVIKHVRP 
      SDAGLYECQ VSTHPATSI FIELRVTEA SAEIIGAPD 
      LHVRAGSSL RLVCTILRS TEPPVYVFW YHDQRMINH 
      DVGVSVTID RTSSVLQLQ DADTTHSGN YTCDPANAI 
      PAYINVHVL NATEEENPA AMLHASSSG ISTALFMTI 
      MLIVAVNIT FSLNS
    CDS sequence
    • >TC010127 RA
      atggcagtg attgtgcat cctttgaac atttacatg 
      tgcctcttc ttcgtcgtc tcggcagaa atgatactc 
      actccttct tcccttatc tgccaagat atggtcaag 
      ttgagccga gataggagg gcgtacgaa gctcatcga 
      agcagagac aatgtggtg aaaccctct tccgggtcc 
      tctcccagc cggggctca gtgtttgca acggccaat 
      tcaacatcg gtggcggcg cagatcggg gggacggcg 
      aagctgccg tgcatcgtc cgcaagttc aacaacggg 
      gttgtgtcc tggatccgc aaggacatc acccctccc 
      acgatcctg acggtgggc ttgggtcca tacattgcc 
      gatgaccgg ttcatggta gagcacgcc cgccacctc 
      caaaactgg gacttggtc ataaaacat gtgcgtccc 
      tccgacgcc ggactttac gaatgtcaa gtctcaaca 
      catcctgcg actagcata tttatcgag ttacgagtc 
      acagaggcc tcagcggaa atcatcggc gctccggat 
      ctgcacgtc cgggcgggc tcctcgctc cgcctcgtc 
      tgcacgatc ctccgctcg acggagccg cccgtctac 
      gtgttctgg tatcacgac cagcggatg atcaatcac 
      gacgtcggc gtctcggtc acgatcgac aggacgagc 
      agcgtcctg cagctgcag gacgccgac acgacgcac 
      agcgggaac tatacctgc gatcctgcg aacgccatc 
      cctgcatac atcaacgtg cacgtcctc aatgccact 
      gaagaagaa aatccagcg gcgatgttg catgcaagc 
      agcagtgga atttcaacc gcgctcttt atgaccata 
      atgctaatt gttgctgta aatataaca ttttcatta 
      aacagttga
    mRNA sequence
    • >TC010127 RA
      tcacttgcc cacttcctc acgcaacaa cagaaaaac 
      ttttattaa gtcggatac gtgccaccg acttcgatt 
      attgccgcc acgacccca gtgtcaaaa ttttattgc 
      ccaacttga ctttcatgc gagctaaaa atgcgcgat 
      aaagatatt catgcatat ggtcgtgtc tggcgtcac 
      ctaacaaga gactttcgc agtgactat gataccaac 
      tagtaccat ctcattaaa gtagaataa catctcgca 
      atctcttcc tgacggcgc cccttttca gcaaattat 
      gaaacaatg cgaacggag cgcctgaaa cacgagaat 
      tgccgtaaa caaagccgt tccgtcgtg atgtaataa 
      taatagtag cggggttgt atatgtgcg acctctttt 
      gtgattgtg cggattgta cgtggagac cattctatt 
      aattatgca cctacaatt aagactgat cgagattat 
      cactcgaac gtcttaacg tctgcgttc gcaaccctc 
      tcgctccac tacccgact caataatgg cagtgattg 
      tgcatcctt tgaacattt acatgtgcc tcttcttcg 
      tcgtctcgg cagaaatga tactcactc cttcttccc 
      ttatctgcc aagatatgg tcaagttga gccgagata 
      ggagggcgt acgaagctc atcgaagca gagacaatg 
      tggtgaaac cctcttccg ggtcctctc ccagccggg 
      gctcagtgt ttgcaacgg ccaattcaa catcggtgg 
      cggcgcaga tcgggggga cggcgaagc tgccgtgca 
      tcgtccgca agttcaaca acggggttg tgtcctgga 
      tccgcaagg acatcaccc ctcccacga tcctgacgg 
      tgggcttgg gtccataca ttgccgatg accggttca 
      tggtagagc acgcccgcc acctccaaa actgggact 
      tggtcataa aacatgtgc gtccctccg acgccggac 
      tttacgaat gtcaagtct caacacatc ctgcgacta 
      gcatattta tcgagttac gagtcacag aggcctcag 
      cggaaatca tcggcgctc cggatctgc acgtccggg 
      cgggctcct cgctccgcc tcgtctgca cgatcctcc 
      gctcgacgg agccgcccg tctacgtgt tctggtatc 
      acgaccagc ggatgatca atcacgacg tcggcgtct 
      cggtcacga tcgacagga cgagcagcg tcctgcagc 
      tgcaggacg ccgacacga cgcacagcg ggaactata 
      cctgcgatc ctgcgaacg ccatccctg catacatca 
      acgtgcacg tcctcaatg ccactgaag aagaaaatc 
      cagcggcga tgttgcatg caagcagca gtggaattt 
      caaccgcgc tctttatga ccataatgc taattgttg 
      ctgtaaata taacatttt cattaaaca gttgattgt 
      cttcgaaat atttgtata aattgtaaa tacctgtac 
      agtctcata aactaccag ctatttata actgtaact 
      attttaggg ttccagttc gaattaatg atgagtatt 
      gactaaaac tacgtagtc atatttgcc tcattgtac 
      tgtgtgtta caataaaaa caagaaaaa taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY