Status
Please wait ...
Gene information
General information
TC010140 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14147450..14151371
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010140
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_08818

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010140 RA
        MSVASFAGP KDTATESVS NTSRKRNSP SVRISKRKL 
        KAPINSDDE GDLESNHSQ STLKENNGT PAPITVQLS 
        LNKPTSLAC SINITTSAP LNSPPRSVL TPSSVSSPP 
        KGVLTPPSE NLPSPNTSS NKNVSPKQT PKKRLFDES 
        NKTNTPSTS KICVLENRV IVTSPLKKS PKNEVKDET 
        KKLNYDVLK VMREFFINV LKYSHLKCL LSKSDQDML 
        YNFYNIEKD YQYTCVKLF LWKQQWYNI FKYVGGIGL 
        KMSDNDVTA MYKCLEEKH FVDTNYMAE ETVTLLNLL 
        SVADLRAIL AQFKLPTAG PRESLIDKL LRNCKTQVT 
        LTCTKSTED LLRERIKER MGYCVRLST NLNRCLRYV 
        FLLNTFTNF SLTVPQDYF KNVTYFNTV FPSCKVETF 
        HIFYTPGEF SKYADAVDL NRELEEAIE KKNNPNILV 
        LCQRAFNLL KQANEEAKN DMREPHLER FTPNAQYIR 
        IITQGLKHI KRHNFGEVQ IWLEYLITQ KYCKHKRGD 
        WYCELALIY TEYLKDYEK AAKLIIKAF KEEKEFLTD 
        IQLQALAHR GRLTNRKTY NLPSLYHDE IAALSPPAC 
        DRDWPQEVL DCESLKSQK SGQKRKYVH RCANGDVEY 
        LSVEQKALK HYETMGFPS GLHCEGSVV IAAFYLLFW 
        DIIYEEYVR GTFISSVQS APLDMHTPY FYKNRERSI 
        QKRLDEIRD KWKNIELRT KLSRAWDLH SHENSVIEA 
        AVPKSDHIF DVIMCIGRP VLAKIFERL VQNFKVYQS 
        GFPDLIVWN VEEGRAKFV EVKGENDKL SIKQKLWLN 
        YLCDSGASV EVCRVEPKA TKKQFKRSV AAACH
      CDS sequence
      • >TC010140 RA
        atgtctgtc gcatctttt gctggcccc aaagacact 
        gcaacagag tcggtttca aacacatca agaaaaaga 
        aatagtccg tctgtaagg atctcgaag aggaaatta 
        aaagcacca atcaactca gatgatgaa ggtgatttg 
        gaaagtaat cacagtcaa agtacatta aaggaaaac 
        aatggaacc ccagctcct attacagtt caactatct 
        ttaaataaa cccacgtct cttgcatgt tcaataaat 
        attacgact tcagctcct ctgaattca ccaccaaga 
        agcgttcta acaccgtca tccgtgagt tcaccacca 
        aaaggtgtt ttaacaccg ccatccgaa aatcttcct 
        tctccaaac acttcatcc aacaaaaat gtatctcca 
        aagcaaaca ccaaagaaa cgacttttt gacgaaagt 
        aacaaaaca aacactcct agtacaagt aaaatctgc 
        gttctagaa aatagagtg atagtgacg tctcctctt 
        aaaaaatca ccaaagaat gaagttaaa gacgaaacg 
        aaaaaactc aactatgat gtgctaaaa gtgatgagg 
        gaattcttc atcaatgtt ctgaaatat agccacttg 
        aagtgcctg ttgagcaag agtgatcaa gacatgctt 
        tataatttt tataacata gagaaggac taccaatac 
        acgtgtgta aaactgttt ttgtggaaa caacagtgg 
        tacaatatt tttaaatat gtgggcgga atcgggtta 
        aaaatgagc gataatgat gtgactgcc atgtacaag 
        tgtttagaa gagaaacat tttgttgat acgaattat 
        atggctgaa gaaacagtc actctatta aatctgttg 
        agtgtagcg gatctgcga gctatttta gcccagttt 
        aaactaccc actgctggg cccagagag tctctgatt 
        gataaacta ctgagaaat tgtaaaact caagtgacc 
        ctcacttgt acgaaaagc acagaagac ctgcttcga 
        gaacggatc aaggagaga atgggatat tgtgtcaga 
        ctctctaca aatctcaac cgttgcctg cgctacgtt 
        tttctgtta aatacattc actaatttt agtttgact 
        gtgccacaa gattatttt aaaaacgtt acgtacttc 
        aacactgtg ttcccctcc tgcaaagtt gagactttt 
        catattttt tatacgccg ggagagttt tcaaagtat 
        gcagacgcc gttgattta aacagagag ttggaggaa 
        gctattgag aaaaagaac aatccaaat attttagta 
        ctgtgtcaa agggcattt aacttgctg aaacaagcg 
        aacgaggaa gccaaaaat gacatgagg gagccacat 
        ttggagcgt tttactcca aacgcccaa tacattcgt 
        atcataaca caaggcctg aaacacatc aaacggcac 
        aatttcggt gaggttcaa atttggttg gaatacctc 
        atcacacag aaatattgc aaacataaa cgtggtgac 
        tggtactgc gagctggca ttgatttat actgaatat 
        ttaaaagat tacgaaaag gccgcaaaa ttaataata 
        aaagcgttc aaggaagag aaagaattt ctgaccgac 
        atacaattg caagcactt gcacacaga ggtaggctt 
        acaaatagg aaaacctac aacttgccc tcgttgtac 
        cacgacgaa atagctgcc ttgtcacca cctgcgtgc 
        gaccgtgat tggccccaa gaggtcttg gactgcgaa 
        tcgttgaaa agccaaaaa tctggccag aagcggaaa 
        tatgttcac cgctgcgcc aacggcgat gtcgagtat 
        ttgtctgtt gagcaaaag gctctgaag cactacgag 
        acgatgggc tttcctagc ggtttacac tgcgagggt 
        agcgtggta attgcggcc ttctatctg ctattctgg 
        gacatcatt tatgaggaa tacgtgcgt ggaacattt 
        atttcaagc gtacaaagt gcccccttg gacatgcat 
        acgccctac ttttacaag aacagagaa cgctcgatt 
        caaaagcga ctggacgag attcgagac aagtggaag 
        aacattgaa ttaaggacg aagttatcg cgcgcttgg 
        gacttacat tcgcacgaa aattctgtg attgaggcc 
        gcggtgcct aaatctgac catattttt gatgtgata 
        atgtgcatt gggcgacct gtgctcgct aaaattttc 
        gaaagactg gtgcaaaat tttaaagtt taccaaagt 
        gggtttccc gacttgatt gtgtggaat gttgaggag 
        ggaagggcg aaatttgtc gaagttaag ggggaaaat 
        gataaactc tcaattaag cagaagttg tggttgaac 
        tatttgtgt gatagcggg gcttctgtg gaagtttgt 
        agagttgaa cccaaagcc acgaaaaaa cagtttaag 
        agaagtgtc gccgcagcg tgccattag
      mRNA sequence
      • >TC010140 RA
        ctcaattat ttttgttct aatttctgt tatgtatta 
        ttatcaata caaattatc tgtcgtggt tttataatt 
        tgaattatg aattgaact aacatgcgc tttcgtgtt 
        gtagttccc taaccttga aaatgttga agtgtttgg 
        tttgatcac agggtttga taagtttgt gatgtcagt 
        gcagaataa taataatgt ctgagttct ctcagtgtg 
        actctggag ggaaaagac ttagtcaaa caaaaatca 
        gctcctact ttacaggct gtgtaaagc gaatcccaa 
        cagaccccc cttttaaga aaaaacgta gccgccgaa 
        caatgtctg tcgcatctt ttgctggcc ccaaagaca 
        ctgcaacag agtcggttt caaacacat caagaaaaa 
        gaaatagtc cgtctgtaa ggatctcga agaggaaat 
        taaaagcac caatcaact cagatgatg aaggtgatt 
        tggaaagta atcacagtc aaagtacat taaaggaaa 
        acaatggaa ccccagctc ctattacag ttcaactat 
        ctttaaata aacccacgt ctcttgcat gttcaataa 
        atattacga cttcagctc ctctgaatt caccaccaa 
        gaagcgttc taacaccgt catccgtga gttcaccac 
        caaaaggtg ttttaacac cgccatccg aaaatcttc 
        cttctccaa acacttcat ccaacaaaa atgtatctc 
        caaagcaaa caccaaaga aacgacttt ttgacgaaa 
        gtaacaaaa caaacactc ctagtacaa gtaaaatct 
        gcgttctag aaaatagag tgatagtga cgtctcctc 
        ttaaaaaat caccaaaga atgaagtta aagacgaaa 
        cgaaaaaac tcaactatg atgtgctaa aagtgatga 
        gggaattct tcatcaatg ttctgaaat atagccact 
        tgaagtgcc tgttgagca agagtgatc aagacatgc 
        tttataatt tttataaca tagagaagg actaccaat 
        acacgtgtg taaaactgt ttttgtgga aacaacagt 
        ggtacaata tttttaaat atgtgggcg gaatcgggt 
        taaaaatga gcgataatg atgtgactg ccatgtaca 
        agtgtttag aagagaaac attttgttg atacgaatt 
        atatggctg aagaaacag tcactctat taaatctgt 
        tgagtgtag cggatctgc gagctattt tagcccagt 
        ttaaactac ccactgctg ggcccagag agtctctga 
        ttgataaac tactgagaa attgtaaaa ctcaagtga 
        ccctcactt gtacgaaaa gcacagaag acctgcttc 
        gagaacgga tcaaggaga gaatgggat attgtgtca 
        gactctcta caaatctca accgttgcc tgcgctacg 
        tttttctgt taaatacat tcactaatt ttagtttga 
        ctgtgccac aagattatt ttaaaaacg ttacgtact 
        tcaacactg tgttcccct cctgcaaag ttgagactt 
        ttcatattt tttatacgc cgggagagt tttcaaagt 
        atgcagacg ccgttgatt taaacagag agttggagg 
        aagctattg agaaaaaga acaatccaa atattttag 
        tactgtgtc aaagggcat ttaacttgc tgaaacaag 
        cgaacgagg aagccaaaa atgacatga gggagccac 
        atttggagc gttttactc caaacgccc aatacattc 
        gtatcataa cacaaggcc tgaaacaca tcaaacggc 
        acaatttcg gtgaggttc aaatttggt tggaatacc 
        tcatcacac agaaatatt gcaaacata aacgtggtg 
        actggtact gcgagctgg cattgattt atactgaat 
        atttaaaag attacgaaa aggccgcaa aattaataa 
        taaaagcgt tcaaggaag agaaagaat ttctgaccg 
        acatacaat tgcaagcac ttgcacaca gaggtaggc 
        ttacaaata ggaaaacct acaacttgc cctcgttgt 
        accacgacg aaatagctg ccttgtcac cacctgcgt 
        gcgaccgtg attggcccc aagaggtct tggactgcg 
        aatcgttga aaagccaaa aatctggcc agaagcgga 
        aatatgttc accgctgcg ccaacggcg atgtcgagt 
        atttgtctg ttgagcaaa aggctctga agcactacg 
        agacgatgg gctttccta gcggtttac actgcgagg 
        gtagcgtgg taattgcgg ccttctatc tgctattct 
        gggacatca tttatgagg aatacgtgc gtggaacat 
        ttatttcaa gcgtacaaa gtgccccct tggacatgc 
        atacgccct acttttaca agaacagag aacgctcga 
        ttcaaaagc gactggacg agattcgag acaagtgga 
        agaacattg aattaagga cgaagttat cgcgcgctt 
        gggacttac attcgcacg aaaattctg tgattgagg 
        ccgcggtgc ctaaatctg accatattt ttgatgtga 
        taatgtgca ttgggcgac ctgtgctcg ctaaaattt 
        tcgaaagac tggtgcaaa attttaaag tttaccaaa 
        gtgggtttc ccgacttga ttgtgtgga atgttgagg 
        agggaaggg cgaaatttg tcgaagtta agggggaaa 
        atgataaac tctcaatta agcagaagt tgtggttga 
        actatttgt gtgatagcg gggcttctg tggaagttt 
        gtagagttg aacccaaag ccacgaaaa aacagttta 
        agagaagtg tcgccgcag cgtgccatt agatattaa 
        agacttata tttttatac gttcttata ccgatttat 
        tatgtaatt tatcttaag tgtatttta tgtttttga 
        gttttgaga attaacgta tttgttatt ttttaataa 
        agaattgtt gaactt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY