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Gene information
General information
TC010153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13927025..13928641
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010153
    iBeetle screen iB_08816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    egg chamber mostly not present - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010153 RA
      MSSRILAPK MARADTLCS QSLLEAGDR PLERPLGGE 
      PGDVKTQFV TREGTYRLM TLSEYSRPN RVGYQTQGQ 
      TPPQVRVSL VTLPDQGER ICFNHGREL YVYVYKGIK 
      KAADLSKPL EKKVYKGTN PTCHDFNIA SASGESVSL 
      LIGFSTGQI QLIDPIKKE LNKLFNEER LIDKTRVTC 
      LRWVPGSRS LFLAAHASG QLYVYNEEL PCGSAAPHY 
      QPFKVGEGF SVSTCKTKS TRNPLFRWL LGTGSAINE 
      LAFSPSGAQ LAIVSQDGQ LRVFQYDSM ELLGTARSY 
      FGGLLCVAW SGDGRLIAV GGEDDLVTV YSMEQKRVV 
      GRAQGHRSW VAAVAFDWF LEAESCYRL GSVGHDTQL 
      CLWELPEDA LTTPSKPKV KRRSDPLQL VGTPACPRL 
      DECPVLEPL VCKKIAHER LTALVFRPD CIITACQDG 
      YVYTWARPP S
    CDS sequence
    • >TC010153 RA
      atgtcttcg cgtattttg gccccgaaa atggcaaga 
      gcagacact ttatgcagt cagtcgctt ctggaagcg 
      ggcgaccgc cccctcgaa cgcccgctc gggggcgag 
      cccggcgac gtgaagacc cagttcgtg acccgcgaa 
      ggcacctac cgtctcatg accctcagc gaatactcg 
      aggcctaac cgagtcggt tatcagact caggggcaa 
      acgccgccc caggtgcgt gtctccctt gtcacgctt 
      ccggaccag ggcgagagg atttgcttc aaccatggg 
      cgggagctg tacgtttac gtgtacaaa gggatcaag 
      aaagcggcc gatttgagc aagcccttg gagaagaag 
      gtgtacaaa gggacgaac ccgacgtgt cacgatttt 
      aacatcgcg tcagcttcg ggcgaaagc gtcagtctc 
      ttgatcggc ttcagcacg ggtcagatc cagctaatt 
      gacccgatt aagaaggaa ttgaacaaa ttgttcaac 
      gaggagcgg cttattgac aaaacgagg gttacgtgc 
      ctgaggtgg gtgccggga agccggtcg ttgttccta 
      gcagcgcac gcctcaggc cagttgtat gtttacaat 
      gaggagctc ccgtgtggc tcggcagcc ccccattac 
      cagccgttc aaagtgggg gagggcttc agtgtgagc 
      acttgcaag acgaaatcg acccggaac ccccttttc 
      cggtggctt ctgggcacc ggaagcgcc attaacgag 
      ctggcgttc agccccagt ggggcccaa ctggccata 
      gtgtcgcaa gatgggcag ttgcgggtg ttccagtac 
      gacagtatg gagctgctg ggcaccgcc cgctcgtac 
      ttcgggggg ctgctgtgc gtggcgtgg tctggcgac 
      gggcgcctg attgcagtg gggggcgaa gacgatttg 
      gtcactgtt tactccatg gagcagaag cgggtcgtc 
      gggagggcg caggggcac cgctcgtgg gtggccgcc 
      gtggcgttt gattggttc ctggaggcg gagtcgtgc 
      tacaggttg gggtcagtg gggcacgac acgcagctg 
      tgtctttgg gagctgccc gaagacgcg ttgacgact 
      ccttcgaag ccgaaggtt aagagaagg tcggatcct 
      ttgcagctg gtggggaca ccggcctgt cccaggctg 
      gacgagtgt ccggtgttg gagccttta gtttgtaaa 
      aaaattgct catgaaagg ttgactgct ttggttttt 
      cgacctgat tgtatcatt acggcgtgc caagacggt 
      tatgtgtat acatgggca aggccccct tcctga
    mRNA sequence
    • >TC010153 RA
      tagaattcg tcatagaat gtcttcgcg tattttggc 
      cccgaaaat ggcaagagc agacacttt atgcagtca 
      gtcgcttct ggaagcggg cgaccgccc cctcgaacg 
      cccgctcgg gggcgagcc cggcgacgt gaagaccca 
      gttcgtgac ccgcgaagg cacctaccg tctcatgac 
      cctcagcga atactcgag gcctaaccg agtcggtta 
      tcagactca ggggcaaac gccgcccca ggtgcgtgt 
      ctcccttgt cacgcttcc ggaccaggg cgagaggat 
      ttgcttcaa ccatgggcg ggagctgta cgtttacgt 
      gtacaaagg gatcaagaa agcggccga tttgagcaa 
      gcccttgga gaagaaggt gtacaaagg gacgaaccc 
      gacgtgtca cgattttaa catcgcgtc agcttcggg 
      cgaaagcgt cagtctctt gatcggctt cagcacggg 
      tcagatcca gctaattga cccgattaa gaaggaatt 
      gaacaaatt gttcaacga ggagcggct tattgacaa 
      aacgagggt tacgtgcct gaggtgggt gccgggaag 
      ccggtcgtt gttcctagc agcgcacgc ctcaggcca 
      gttgtatgt ttacaatga ggagctccc gtgtggctc 
      ggcagcccc ccattacca gccgttcaa agtggggga 
      gggcttcag tgtgagcac ttgcaagac gaaatcgac 
      ccggaaccc ccttttccg gtggcttct gggcaccgg 
      aagcgccat taacgagct ggcgttcag ccccagtgg 
      ggcccaact ggccatagt gtcgcaaga tgggcagtt 
      gcgggtgtt ccagtacga cagtatgga gctgctggg 
      caccgcccg ctcgtactt cggggggct gctgtgcgt 
      ggcgtggtc tggcgacgg gcgcctgat tgcagtggg 
      gggcgaaga cgatttggt cactgttta ctccatgga 
      gcagaagcg ggtcgtcgg gagggcgca ggggcaccg 
      ctcgtgggt ggccgccgt ggcgtttga ttggttcct 
      ggaggcgga gtcgtgcta caggttggg gtcagtggg 
      gcacgacac gcagctgtg tctttggga gctgcccga 
      agacgcgtt gacgactcc ttcgaagcc gaaggttaa 
      gagaaggtc ggatccttt gcagctggt ggggacacc 
      ggcctgtcc caggctgga cgagtgtcc ggtgttgga 
      gcctttagt ttgtaaaaa aattgctca tgaaaggtt 
      gactgcttt ggtttttcg acctgattg tatcattac 
      ggcgtgcca agacggtta tgtgtatac atgggcaag 
      gcccccttc ctgaatatg tcgtatttt tctaattag 
      atttttatt caactttta tacattgta ggggcaata 
      acaggtgga taaaactgt ggaaccaaa gtgtaaaat 
      agcagacgc ttggttgca ttttttcac ctgatagca 
      tgtaagtgt aattgatgg atgtagaat ctgttgcat 
      aattggtgt gatttttcg taattttat cattcgtaa 
      atatatgaa catattttg aaacttgtg tttcatttt 
      caccctttt tccaaataa aatccttac aaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY