Status
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Gene information
General information
TC010153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13927025..13928641
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010153
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    egg chamber mostly not present - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010153 RA
      MSSRILAPK MARADTLCS QSLLEAGDR PLERPLGGE 
PGDVKTQFV TREGTYRLM TLSEYSRPN RVGYQTQGQ 
TPPQVRVSL VTLPDQGER ICFNHGREL YVYVYKGIK 
KAADLSKPL EKKVYKGTN PTCHDFNIA SASGESVSL 
LIGFSTGQI QLIDPIKKE LNKLFNEER LIDKTRVTC 
LRWVPGSRS LFLAAHASG QLYVYNEEL PCGSAAPHY 
QPFKVGEGF SVSTCKTKS TRNPLFRWL LGTGSAINE 
LAFSPSGAQ LAIVSQDGQ LRVFQYDSM ELLGTARSY 
FGGLLCVAW SGDGRLIAV GGEDDLVTV YSMEQKRVV 
GRAQGHRSW VAAVAFDWF LEAESCYRL GSVGHDTQL 
CLWELPEDA LTTPSKPKV KRRSDPLQL VGTPACPRL 
DECPVLEPL VCKKIAHER LTALVFRPD CIITACQDG 
YVYTWARPP S
    CDS sequence
    • >TC010153 RA
      atgtcttcg cgtattttg gccccgaaa atggcaaga 
gcagacact ttatgcagt cagtcgctt ctggaagcg 
ggcgaccgc cccctcgaa cgcccgctc gggggcgag 
cccggcgac gtgaagacc cagttcgtg acccgcgaa 
ggcacctac cgtctcatg accctcagc gaatactcg 
aggcctaac cgagtcggt tatcagact caggggcaa 
acgccgccc caggtgcgt gtctccctt gtcacgctt 
ccggaccag ggcgagagg atttgcttc aaccatggg 
cgggagctg tacgtttac gtgtacaaa gggatcaag 
aaagcggcc gatttgagc aagcccttg gagaagaag 
gtgtacaaa gggacgaac ccgacgtgt cacgatttt 
aacatcgcg tcagcttcg ggcgaaagc gtcagtctc 
ttgatcggc ttcagcacg ggtcagatc cagctaatt 
gacccgatt aagaaggaa ttgaacaaa ttgttcaac 
gaggagcgg cttattgac aaaacgagg gttacgtgc 
ctgaggtgg gtgccggga agccggtcg ttgttccta 
gcagcgcac gcctcaggc cagttgtat gtttacaat 
gaggagctc ccgtgtggc tcggcagcc ccccattac 
cagccgttc aaagtgggg gagggcttc agtgtgagc 
acttgcaag acgaaatcg acccggaac ccccttttc 
cggtggctt ctgggcacc ggaagcgcc attaacgag 
ctggcgttc agccccagt ggggcccaa ctggccata 
gtgtcgcaa gatgggcag ttgcgggtg ttccagtac 
gacagtatg gagctgctg ggcaccgcc cgctcgtac 
ttcgggggg ctgctgtgc gtggcgtgg tctggcgac 
gggcgcctg attgcagtg gggggcgaa gacgatttg 
gtcactgtt tactccatg gagcagaag cgggtcgtc 
gggagggcg caggggcac cgctcgtgg gtggccgcc 
gtggcgttt gattggttc ctggaggcg gagtcgtgc 
tacaggttg gggtcagtg gggcacgac acgcagctg 
tgtctttgg gagctgccc gaagacgcg ttgacgact 
ccttcgaag ccgaaggtt aagagaagg tcggatcct 
ttgcagctg gtggggaca ccggcctgt cccaggctg 
gacgagtgt ccggtgttg gagccttta gtttgtaaa 
aaaattgct catgaaagg ttgactgct ttggttttt 
cgacctgat tgtatcatt acggcgtgc caagacggt 
tatgtgtat acatgggca aggccccct tcctga
    mRNA sequence
    • >TC010153 RA
      tagaattcg tcatagaat gtcttcgcg tattttggc 
cccgaaaat ggcaagagc agacacttt atgcagtca 
gtcgcttct ggaagcggg cgaccgccc cctcgaacg 
cccgctcgg gggcgagcc cggcgacgt gaagaccca 
gttcgtgac ccgcgaagg cacctaccg tctcatgac 
cctcagcga atactcgag gcctaaccg agtcggtta 
tcagactca ggggcaaac gccgcccca ggtgcgtgt 
ctcccttgt cacgcttcc ggaccaggg cgagaggat 
ttgcttcaa ccatgggcg ggagctgta cgtttacgt 
gtacaaagg gatcaagaa agcggccga tttgagcaa 
gcccttgga gaagaaggt gtacaaagg gacgaaccc 
gacgtgtca cgattttaa catcgcgtc agcttcggg 
cgaaagcgt cagtctctt gatcggctt cagcacggg 
tcagatcca gctaattga cccgattaa gaaggaatt 
gaacaaatt gttcaacga ggagcggct tattgacaa 
aacgagggt tacgtgcct gaggtgggt gccgggaag 
ccggtcgtt gttcctagc agcgcacgc ctcaggcca 
gttgtatgt ttacaatga ggagctccc gtgtggctc 
ggcagcccc ccattacca gccgttcaa agtggggga 
gggcttcag tgtgagcac ttgcaagac gaaatcgac 
ccggaaccc ccttttccg gtggcttct gggcaccgg 
aagcgccat taacgagct ggcgttcag ccccagtgg 
ggcccaact ggccatagt gtcgcaaga tgggcagtt 
gcgggtgtt ccagtacga cagtatgga gctgctggg 
caccgcccg ctcgtactt cggggggct gctgtgcgt 
ggcgtggtc tggcgacgg gcgcctgat tgcagtggg 
gggcgaaga cgatttggt cactgttta ctccatgga 
gcagaagcg ggtcgtcgg gagggcgca ggggcaccg 
ctcgtgggt ggccgccgt ggcgtttga ttggttcct 
ggaggcgga gtcgtgcta caggttggg gtcagtggg 
gcacgacac gcagctgtg tctttggga gctgcccga 
agacgcgtt gacgactcc ttcgaagcc gaaggttaa 
gagaaggtc ggatccttt gcagctggt ggggacacc 
ggcctgtcc caggctgga cgagtgtcc ggtgttgga 
gcctttagt ttgtaaaaa aattgctca tgaaaggtt 
gactgcttt ggtttttcg acctgattg tatcattac 
ggcgtgcca agacggtta tgtgtatac atgggcaag 
gcccccttc ctgaatatg tcgtatttt tctaattag 
atttttatt caactttta tacattgta ggggcaata 
acaggtgga taaaactgt ggaaccaaa gtgtaaaat 
agcagacgc ttggttgca ttttttcac ctgatagca 
tgtaagtgt aattgatgg atgtagaat ctgttgcat 
aattggtgt gatttttcg taattttat cattcgtaa 
atatatgaa catattttg aaacttgtg tttcatttt 
caccctttt tccaaataa aatccttac aaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY