| General information | |||
| TC010162 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 13782229..13787266 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010162 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08813 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC010162 RA
MVMSGMLPS FTPPVVKRL LGWKKGTDA DDRWCEKAV KSLVKKLKK SGALEELER AISSQNHNT KCVTIPRVR PAAELGNVQ QQRKGLPHV IYCRLWRWP ELQSHHELR PLENCEYAY NLKKEEVCV NPYHYTRIE SPALPAILV PRHPLSDEN NLFPHSLED LSTSVPENT SFPHNPNSL GLHMQHSGG YMDAVGICP NGAPANMES PHNAAPPTE TPPPGYMSE DGDPIDPND NMSMSRITP SPPVDAQPV LYCEPAFWC SISYYELNT RVGETFHAS QPSITVDGF TDPSNSERF CLGLLSNVN RNTVVEQTR RHIGKGVRL YYIGGEVFA ECLSDSSIF VQSPNCNQR YGWHPATVC KIPPGCNLK IFNNQEFAA LLSQSVSQG FEAVYQLTR MCTIRMSFV KGWGAEYRR QTVTSTPCW IELHLNGPL QWLDRVLTQ MGSPRLPCS SMS
- >TC010162 RA
atggtgatg agtgggatg ctcccgtcg ttcacgccg cccgtcgta aagcgatta ctgggatgg aaaaaaggc actgacgcc gatgacaga tggtgtgag aaggccgtc aagagttta gtcaagaaa ctgaaaaaa tcgggcgct ttggaggag ctagaacga gcgatttcg tcacagaat cataatacg aaatgcgtc acaattcct agagttcgg ccggctgct gaattaggc aacgtccag caacagagg aagggtttg ccccacgtt atttactgc cggctgtgg agatggcct gagctgcaa tcgcaccat gaactcaga cctctcgaa aactgcgaa tacgcgtac aatttaaaa aaggaggaa gtctgtgtc aatccatac cactatact agaattgaa agcccagcc ctcccagcc attttagtc ccccgccat ccactcagc gacgaaaac aacttgttt ccgcattcg ctggaagac ctcagcacg tctgtgccc gaaaacacg tcatttcct cacaatcca aatagtcta ggtttgcat atgcaacat tctgggggt tacatggat gcggttggt atttgcccg aatggcgct cctgcaaat atggaatct ccccacaat gctgcccca cctactgaa actcctcca cctggttat atgtccgaa gatggtgat ccgattgat cctaacgat aacatgagc atgtccagg ataacgcct agtcctccc gtggacgcg cagcctgta ctttactgt gaacccgct ttttggtgc agtatcagc tactacgag ctgaacaca cgagtaggg gaaacgttt cacgctagc caaccttca atcacagtg gatgggttc acagatccg agcaattcg gaaagattt tgtctgggg ctgttatct aacgtaaat cggaacacc gtcgtcgag caaacccgg cggcacata ggaaagggt gtaagactt tattatata ggtggcgaa gtttttgct gaatgtttg agcgactcg agtattttt gtgcagtca ccaaattgt aatcaacgg tatgggtgg catccagcc accgtgtgt aaaattcca ccaggatgc aatttaaaa attttcaac aaccaagaa ttcgcagct cttttgtca cagtccgtg agccagggt tttgaagct gtttatcaa ttgaccagg atgtgtacg ataaggatg tcgtttgtt aagggttgg ggggcggag tataggcga caaacagtg acgtcaaca ccgtgttgg attgagctt cacttgaat ggacctctt cagtggtta gatcgtgta ctaacccaa atgggctca cctcggtta ccatgcagt tcaatgtcg taa
- >TC010162 RA
attgggttt aaaaccttg tctttaagg caatattcg aggaatgta aacagtgtc ttggaccac gttttcgcg acgccactg gtttggaat agttttgtg tgcgcgcat gcgtaaaaa ttgttaaca caacacacg tgcgtcctt ttttgttag tttttaaat gtggggaaa gaagacaga tgacgcaaa tgaggctgt gcttatttc gcgtggagc gacaatcat cgtccgcct gtcaaagaa caatatggc agtggtaaa gcaataatt tttgtaaaa tttttggca agttttctc tgtgaaaat tggtgatat ccgtgtgcg tgaattttt gtaactcgt ggttgtttg atggtgatg agtgggatg ctcccgtcg ttcacgccg cccgtcgta aagcgatta ctgggatgg aaaaaaggc actgacgcc gatgacaga tggtgtgag aaggccgtc aagagttta gtcaagaaa ctgaaaaaa tcgggcgct ttggaggag ctagaacga gcgatttcg tcacagaat cataatacg aaatgcgtc acaattcct agagttcgg ccggctgct gaattaggc aacgtccag caacagagg aagggtttg ccccacgtt atttactgc cggctgtgg agatggcct gagctgcaa tcgcaccat gaactcaga cctctcgaa aactgcgaa tacgcgtac aatttaaaa aaggaggaa gtctgtgtc aatccatac cactatact agaattgaa agcccagcc ctcccagcc attttagtc ccccgccat ccactcagc gacgaaaac aacttgttt ccgcattcg ctggaagac ctcagcacg tctgtgccc gaaaacacg tcatttcct cacaatcca aatagtcta ggtttgcat atgcaacat tctgggggt tacatggat gcggttggt atttgcccg aatggcgct cctgcaaat atggaatct ccccacaat gctgcccca cctactgaa actcctcca cctggttat atgtccgaa gatggtgat ccgattgat cctaacgat aacatgagc atgtccagg ataacgcct agtcctccc gtggacgcg cagcctgta ctttactgt gaacccgct ttttggtgc agtatcagc tactacgag ctgaacaca cgagtaggg gaaacgttt cacgctagc caaccttca atcacagtg gatgggttc acagatccg agcaattcg gaaagattt tgtctgggg ctgttatct aacgtaaat cggaacacc gtcgtcgag caaacccgg cggcacata ggaaagggt gtaagactt tattatata ggtggcgaa gtttttgct gaatgtttg agcgactcg agtattttt gtgcagtca ccaaattgt aatcaacgg tatgggtgg catccagcc accgtgtgt aaaattcca ccaggatgc aatttaaaa attttcaac aaccaagaa ttcgcagct cttttgtca cagtccgtg agccagggt tttgaagct gtttatcaa ttgaccagg atgtgtacg ataaggatg tcgtttgtt aagggttgg ggggcggag tataggcga caaacagtg acgtcaaca ccgtgttgg attgagctt cacttgaat ggacctctt cagtggtta gatcgtgta ctaacccaa atgggctca cctcggtta ccatgcagt tcaatgtcg taaagtact gagtgaaag gagtgtcaa atgcggtcc ggttttcag tcgtaactc agctattat tttcattgg gaaaaattg ttgcaaatc aaattacgt tttgggtga tataaaaaa gtgtggcga tttttttac atatagtta ctatttagc tttacaaaa agttttatt ttacattta gagagattt attttatat tgctgagtt ttaaactgt tggtgttgt tagtgttgc aaatatatt acactttac aggacacaa tttcaattt tggcgtagt caggcaaaa gctaaaagt gcccacctg gcacttccg tggccctga ccggtccag gtgctaaca tacagtaaa tttactgta ctttattaa gttataata tttcattag accctacat tttttaatt ttgaactat catgcgttc tttttttta ttattcgtt catttgtcg catttagct tcgaaaaac cgaacatga cgtttaatt gagtgggaa agtgagatt tgcttaagg ttctataca gtcaaaatt gaaaaaaaa aacattttg ctattccaa tttattagc aaagttcct atgttacgc cactgcttc ttccacatc aagcttgag agcaattta ttgggctcc ccaggtgtg tacttttgg cttttggac acctctgaa acaactttt ttatgtatt ataaaagac tgaatgcta gaataactg ttttaatcc catccagtt aatacgtaa atattttat ctaattatg gtatttgct ttggtaatt gtttgtcag ttcattgtg cgctaaact tgaagttgt tccagaaat ttatttcta agattagaa ctgtgttga tcaataaac tgacccagg attatttgt tgtaaatga ggacataca aggcgggcc ttgtatcca gtcacatcc agcaagtgt tcccacttg aagcattaa aataaaaat ctgatagtc acgatattg agaacaatg ctccttgtg gagacattt tttgtacgt gaccatacc ctgtaattc aattgttta accatgttg tggaccttg aaatgattg caagtatta aattggttt aagtacaaa gtatacagg tcacagcat aaataaagg aacaaaatt ggtgctgct gaaattttt gcaattaat tatgttgtg catttgcag cagtaacaa cagttgttt ttaagctgt attgatgga gttgttttc gtgtgaatt cgatgtcac ataccgtga tgtttaggt tttattttg tagtgtttg ttacaagtt ttttagctg atccatctt gtgaatcac aattgatgt tataagctc atatttgta ctatattat ttcgtttat tgcataagt atagtttgt atctggcaa ttaccaaat tatatgcat tttacttga cgaaaaata tatagcc
