Status
Please wait ...
Gene information
General information
TC010162 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13782229..13787266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010162
    iBeetle screen iB_08813

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010162 RA
      MVMSGMLPS FTPPVVKRL LGWKKGTDA DDRWCEKAV 
      KSLVKKLKK SGALEELER AISSQNHNT KCVTIPRVR 
      PAAELGNVQ QQRKGLPHV IYCRLWRWP ELQSHHELR 
      PLENCEYAY NLKKEEVCV NPYHYTRIE SPALPAILV 
      PRHPLSDEN NLFPHSLED LSTSVPENT SFPHNPNSL 
      GLHMQHSGG YMDAVGICP NGAPANMES PHNAAPPTE 
      TPPPGYMSE DGDPIDPND NMSMSRITP SPPVDAQPV 
      LYCEPAFWC SISYYELNT RVGETFHAS QPSITVDGF 
      TDPSNSERF CLGLLSNVN RNTVVEQTR RHIGKGVRL 
      YYIGGEVFA ECLSDSSIF VQSPNCNQR YGWHPATVC 
      KIPPGCNLK IFNNQEFAA LLSQSVSQG FEAVYQLTR 
      MCTIRMSFV KGWGAEYRR QTVTSTPCW IELHLNGPL 
      QWLDRVLTQ MGSPRLPCS SMS
    CDS sequence
    • >TC010162 RA
      atggtgatg agtgggatg ctcccgtcg ttcacgccg 
      cccgtcgta aagcgatta ctgggatgg aaaaaaggc 
      actgacgcc gatgacaga tggtgtgag aaggccgtc 
      aagagttta gtcaagaaa ctgaaaaaa tcgggcgct 
      ttggaggag ctagaacga gcgatttcg tcacagaat 
      cataatacg aaatgcgtc acaattcct agagttcgg 
      ccggctgct gaattaggc aacgtccag caacagagg 
      aagggtttg ccccacgtt atttactgc cggctgtgg 
      agatggcct gagctgcaa tcgcaccat gaactcaga 
      cctctcgaa aactgcgaa tacgcgtac aatttaaaa 
      aaggaggaa gtctgtgtc aatccatac cactatact 
      agaattgaa agcccagcc ctcccagcc attttagtc 
      ccccgccat ccactcagc gacgaaaac aacttgttt 
      ccgcattcg ctggaagac ctcagcacg tctgtgccc 
      gaaaacacg tcatttcct cacaatcca aatagtcta 
      ggtttgcat atgcaacat tctgggggt tacatggat 
      gcggttggt atttgcccg aatggcgct cctgcaaat 
      atggaatct ccccacaat gctgcccca cctactgaa 
      actcctcca cctggttat atgtccgaa gatggtgat 
      ccgattgat cctaacgat aacatgagc atgtccagg 
      ataacgcct agtcctccc gtggacgcg cagcctgta 
      ctttactgt gaacccgct ttttggtgc agtatcagc 
      tactacgag ctgaacaca cgagtaggg gaaacgttt 
      cacgctagc caaccttca atcacagtg gatgggttc 
      acagatccg agcaattcg gaaagattt tgtctgggg 
      ctgttatct aacgtaaat cggaacacc gtcgtcgag 
      caaacccgg cggcacata ggaaagggt gtaagactt 
      tattatata ggtggcgaa gtttttgct gaatgtttg 
      agcgactcg agtattttt gtgcagtca ccaaattgt 
      aatcaacgg tatgggtgg catccagcc accgtgtgt 
      aaaattcca ccaggatgc aatttaaaa attttcaac 
      aaccaagaa ttcgcagct cttttgtca cagtccgtg 
      agccagggt tttgaagct gtttatcaa ttgaccagg 
      atgtgtacg ataaggatg tcgtttgtt aagggttgg 
      ggggcggag tataggcga caaacagtg acgtcaaca 
      ccgtgttgg attgagctt cacttgaat ggacctctt 
      cagtggtta gatcgtgta ctaacccaa atgggctca 
      cctcggtta ccatgcagt tcaatgtcg taa
    mRNA sequence
    • >TC010162 RA
      attgggttt aaaaccttg tctttaagg caatattcg 
      aggaatgta aacagtgtc ttggaccac gttttcgcg 
      acgccactg gtttggaat agttttgtg tgcgcgcat 
      gcgtaaaaa ttgttaaca caacacacg tgcgtcctt 
      ttttgttag tttttaaat gtggggaaa gaagacaga 
      tgacgcaaa tgaggctgt gcttatttc gcgtggagc 
      gacaatcat cgtccgcct gtcaaagaa caatatggc 
      agtggtaaa gcaataatt tttgtaaaa tttttggca 
      agttttctc tgtgaaaat tggtgatat ccgtgtgcg 
      tgaattttt gtaactcgt ggttgtttg atggtgatg 
      agtgggatg ctcccgtcg ttcacgccg cccgtcgta 
      aagcgatta ctgggatgg aaaaaaggc actgacgcc 
      gatgacaga tggtgtgag aaggccgtc aagagttta 
      gtcaagaaa ctgaaaaaa tcgggcgct ttggaggag 
      ctagaacga gcgatttcg tcacagaat cataatacg 
      aaatgcgtc acaattcct agagttcgg ccggctgct 
      gaattaggc aacgtccag caacagagg aagggtttg 
      ccccacgtt atttactgc cggctgtgg agatggcct 
      gagctgcaa tcgcaccat gaactcaga cctctcgaa 
      aactgcgaa tacgcgtac aatttaaaa aaggaggaa 
      gtctgtgtc aatccatac cactatact agaattgaa 
      agcccagcc ctcccagcc attttagtc ccccgccat 
      ccactcagc gacgaaaac aacttgttt ccgcattcg 
      ctggaagac ctcagcacg tctgtgccc gaaaacacg 
      tcatttcct cacaatcca aatagtcta ggtttgcat 
      atgcaacat tctgggggt tacatggat gcggttggt 
      atttgcccg aatggcgct cctgcaaat atggaatct 
      ccccacaat gctgcccca cctactgaa actcctcca 
      cctggttat atgtccgaa gatggtgat ccgattgat 
      cctaacgat aacatgagc atgtccagg ataacgcct 
      agtcctccc gtggacgcg cagcctgta ctttactgt 
      gaacccgct ttttggtgc agtatcagc tactacgag 
      ctgaacaca cgagtaggg gaaacgttt cacgctagc 
      caaccttca atcacagtg gatgggttc acagatccg 
      agcaattcg gaaagattt tgtctgggg ctgttatct 
      aacgtaaat cggaacacc gtcgtcgag caaacccgg 
      cggcacata ggaaagggt gtaagactt tattatata 
      ggtggcgaa gtttttgct gaatgtttg agcgactcg 
      agtattttt gtgcagtca ccaaattgt aatcaacgg 
      tatgggtgg catccagcc accgtgtgt aaaattcca 
      ccaggatgc aatttaaaa attttcaac aaccaagaa 
      ttcgcagct cttttgtca cagtccgtg agccagggt 
      tttgaagct gtttatcaa ttgaccagg atgtgtacg 
      ataaggatg tcgtttgtt aagggttgg ggggcggag 
      tataggcga caaacagtg acgtcaaca ccgtgttgg 
      attgagctt cacttgaat ggacctctt cagtggtta 
      gatcgtgta ctaacccaa atgggctca cctcggtta 
      ccatgcagt tcaatgtcg taaagtact gagtgaaag 
      gagtgtcaa atgcggtcc ggttttcag tcgtaactc 
      agctattat tttcattgg gaaaaattg ttgcaaatc 
      aaattacgt tttgggtga tataaaaaa gtgtggcga 
      tttttttac atatagtta ctatttagc tttacaaaa 
      agttttatt ttacattta gagagattt attttatat 
      tgctgagtt ttaaactgt tggtgttgt tagtgttgc 
      aaatatatt acactttac aggacacaa tttcaattt 
      tggcgtagt caggcaaaa gctaaaagt gcccacctg 
      gcacttccg tggccctga ccggtccag gtgctaaca 
      tacagtaaa tttactgta ctttattaa gttataata 
      tttcattag accctacat tttttaatt ttgaactat 
      catgcgttc tttttttta ttattcgtt catttgtcg 
      catttagct tcgaaaaac cgaacatga cgtttaatt 
      gagtgggaa agtgagatt tgcttaagg ttctataca 
      gtcaaaatt gaaaaaaaa aacattttg ctattccaa 
      tttattagc aaagttcct atgttacgc cactgcttc 
      ttccacatc aagcttgag agcaattta ttgggctcc 
      ccaggtgtg tacttttgg cttttggac acctctgaa 
      acaactttt ttatgtatt ataaaagac tgaatgcta 
      gaataactg ttttaatcc catccagtt aatacgtaa 
      atattttat ctaattatg gtatttgct ttggtaatt 
      gtttgtcag ttcattgtg cgctaaact tgaagttgt 
      tccagaaat ttatttcta agattagaa ctgtgttga 
      tcaataaac tgacccagg attatttgt tgtaaatga 
      ggacataca aggcgggcc ttgtatcca gtcacatcc 
      agcaagtgt tcccacttg aagcattaa aataaaaat 
      ctgatagtc acgatattg agaacaatg ctccttgtg 
      gagacattt tttgtacgt gaccatacc ctgtaattc 
      aattgttta accatgttg tggaccttg aaatgattg 
      caagtatta aattggttt aagtacaaa gtatacagg 
      tcacagcat aaataaagg aacaaaatt ggtgctgct 
      gaaattttt gcaattaat tatgttgtg catttgcag 
      cagtaacaa cagttgttt ttaagctgt attgatgga 
      gttgttttc gtgtgaatt cgatgtcac ataccgtga 
      tgtttaggt tttattttg tagtgtttg ttacaagtt 
      ttttagctg atccatctt gtgaatcac aattgatgt 
      tataagctc atatttgta ctatattat ttcgtttat 
      tgcataagt atagtttgt atctggcaa ttaccaaat 
      tatatgcat tttacttga cgaaaaata tatagcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY