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Gene information
General information
TC010162 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13782229..13787266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010162
    iBeetle screen iB_08813

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010162 RA
      MVMSGMLPS FTPPVVKRL LGWKKGTDA DDRWCEKAV 
KSLVKKLKK SGALEELER AISSQNHNT KCVTIPRVR 
PAAELGNVQ QQRKGLPHV IYCRLWRWP ELQSHHELR 
PLENCEYAY NLKKEEVCV NPYHYTRIE SPALPAILV 
PRHPLSDEN NLFPHSLED LSTSVPENT SFPHNPNSL 
GLHMQHSGG YMDAVGICP NGAPANMES PHNAAPPTE 
TPPPGYMSE DGDPIDPND NMSMSRITP SPPVDAQPV 
LYCEPAFWC SISYYELNT RVGETFHAS QPSITVDGF 
TDPSNSERF CLGLLSNVN RNTVVEQTR RHIGKGVRL 
YYIGGEVFA ECLSDSSIF VQSPNCNQR YGWHPATVC 
KIPPGCNLK IFNNQEFAA LLSQSVSQG FEAVYQLTR 
MCTIRMSFV KGWGAEYRR QTVTSTPCW IELHLNGPL 
QWLDRVLTQ MGSPRLPCS SMS
    CDS sequence
    • >TC010162 RA
      atggtgatg agtgggatg ctcccgtcg ttcacgccg 
cccgtcgta aagcgatta ctgggatgg aaaaaaggc 
actgacgcc gatgacaga tggtgtgag aaggccgtc 
aagagttta gtcaagaaa ctgaaaaaa tcgggcgct 
ttggaggag ctagaacga gcgatttcg tcacagaat 
cataatacg aaatgcgtc acaattcct agagttcgg 
ccggctgct gaattaggc aacgtccag caacagagg 
aagggtttg ccccacgtt atttactgc cggctgtgg 
agatggcct gagctgcaa tcgcaccat gaactcaga 
cctctcgaa aactgcgaa tacgcgtac aatttaaaa 
aaggaggaa gtctgtgtc aatccatac cactatact 
agaattgaa agcccagcc ctcccagcc attttagtc 
ccccgccat ccactcagc gacgaaaac aacttgttt 
ccgcattcg ctggaagac ctcagcacg tctgtgccc 
gaaaacacg tcatttcct cacaatcca aatagtcta 
ggtttgcat atgcaacat tctgggggt tacatggat 
gcggttggt atttgcccg aatggcgct cctgcaaat 
atggaatct ccccacaat gctgcccca cctactgaa 
actcctcca cctggttat atgtccgaa gatggtgat 
ccgattgat cctaacgat aacatgagc atgtccagg 
ataacgcct agtcctccc gtggacgcg cagcctgta 
ctttactgt gaacccgct ttttggtgc agtatcagc 
tactacgag ctgaacaca cgagtaggg gaaacgttt 
cacgctagc caaccttca atcacagtg gatgggttc 
acagatccg agcaattcg gaaagattt tgtctgggg 
ctgttatct aacgtaaat cggaacacc gtcgtcgag 
caaacccgg cggcacata ggaaagggt gtaagactt 
tattatata ggtggcgaa gtttttgct gaatgtttg 
agcgactcg agtattttt gtgcagtca ccaaattgt 
aatcaacgg tatgggtgg catccagcc accgtgtgt 
aaaattcca ccaggatgc aatttaaaa attttcaac 
aaccaagaa ttcgcagct cttttgtca cagtccgtg 
agccagggt tttgaagct gtttatcaa ttgaccagg 
atgtgtacg ataaggatg tcgtttgtt aagggttgg 
ggggcggag tataggcga caaacagtg acgtcaaca 
ccgtgttgg attgagctt cacttgaat ggacctctt 
cagtggtta gatcgtgta ctaacccaa atgggctca 
cctcggtta ccatgcagt tcaatgtcg taa
    mRNA sequence
    • >TC010162 RA
      attgggttt aaaaccttg tctttaagg caatattcg 
aggaatgta aacagtgtc ttggaccac gttttcgcg 
acgccactg gtttggaat agttttgtg tgcgcgcat 
gcgtaaaaa ttgttaaca caacacacg tgcgtcctt 
ttttgttag tttttaaat gtggggaaa gaagacaga 
tgacgcaaa tgaggctgt gcttatttc gcgtggagc 
gacaatcat cgtccgcct gtcaaagaa caatatggc 
agtggtaaa gcaataatt tttgtaaaa tttttggca 
agttttctc tgtgaaaat tggtgatat ccgtgtgcg 
tgaattttt gtaactcgt ggttgtttg atggtgatg 
agtgggatg ctcccgtcg ttcacgccg cccgtcgta 
aagcgatta ctgggatgg aaaaaaggc actgacgcc 
gatgacaga tggtgtgag aaggccgtc aagagttta 
gtcaagaaa ctgaaaaaa tcgggcgct ttggaggag 
ctagaacga gcgatttcg tcacagaat cataatacg 
aaatgcgtc acaattcct agagttcgg ccggctgct 
gaattaggc aacgtccag caacagagg aagggtttg 
ccccacgtt atttactgc cggctgtgg agatggcct 
gagctgcaa tcgcaccat gaactcaga cctctcgaa 
aactgcgaa tacgcgtac aatttaaaa aaggaggaa 
gtctgtgtc aatccatac cactatact agaattgaa 
agcccagcc ctcccagcc attttagtc ccccgccat 
ccactcagc gacgaaaac aacttgttt ccgcattcg 
ctggaagac ctcagcacg tctgtgccc gaaaacacg 
tcatttcct cacaatcca aatagtcta ggtttgcat 
atgcaacat tctgggggt tacatggat gcggttggt 
atttgcccg aatggcgct cctgcaaat atggaatct 
ccccacaat gctgcccca cctactgaa actcctcca 
cctggttat atgtccgaa gatggtgat ccgattgat 
cctaacgat aacatgagc atgtccagg ataacgcct 
agtcctccc gtggacgcg cagcctgta ctttactgt 
gaacccgct ttttggtgc agtatcagc tactacgag 
ctgaacaca cgagtaggg gaaacgttt cacgctagc 
caaccttca atcacagtg gatgggttc acagatccg 
agcaattcg gaaagattt tgtctgggg ctgttatct 
aacgtaaat cggaacacc gtcgtcgag caaacccgg 
cggcacata ggaaagggt gtaagactt tattatata 
ggtggcgaa gtttttgct gaatgtttg agcgactcg 
agtattttt gtgcagtca ccaaattgt aatcaacgg 
tatgggtgg catccagcc accgtgtgt aaaattcca 
ccaggatgc aatttaaaa attttcaac aaccaagaa 
ttcgcagct cttttgtca cagtccgtg agccagggt 
tttgaagct gtttatcaa ttgaccagg atgtgtacg 
ataaggatg tcgtttgtt aagggttgg ggggcggag 
tataggcga caaacagtg acgtcaaca ccgtgttgg 
attgagctt cacttgaat ggacctctt cagtggtta 
gatcgtgta ctaacccaa atgggctca cctcggtta 
ccatgcagt tcaatgtcg taaagtact gagtgaaag 
gagtgtcaa atgcggtcc ggttttcag tcgtaactc 
agctattat tttcattgg gaaaaattg ttgcaaatc 
aaattacgt tttgggtga tataaaaaa gtgtggcga 
tttttttac atatagtta ctatttagc tttacaaaa 
agttttatt ttacattta gagagattt attttatat 
tgctgagtt ttaaactgt tggtgttgt tagtgttgc 
aaatatatt acactttac aggacacaa tttcaattt 
tggcgtagt caggcaaaa gctaaaagt gcccacctg 
gcacttccg tggccctga ccggtccag gtgctaaca 
tacagtaaa tttactgta ctttattaa gttataata 
tttcattag accctacat tttttaatt ttgaactat 
catgcgttc tttttttta ttattcgtt catttgtcg 
catttagct tcgaaaaac cgaacatga cgtttaatt 
gagtgggaa agtgagatt tgcttaagg ttctataca 
gtcaaaatt gaaaaaaaa aacattttg ctattccaa 
tttattagc aaagttcct atgttacgc cactgcttc 
ttccacatc aagcttgag agcaattta ttgggctcc 
ccaggtgtg tacttttgg cttttggac acctctgaa 
acaactttt ttatgtatt ataaaagac tgaatgcta 
gaataactg ttttaatcc catccagtt aatacgtaa 
atattttat ctaattatg gtatttgct ttggtaatt 
gtttgtcag ttcattgtg cgctaaact tgaagttgt 
tccagaaat ttatttcta agattagaa ctgtgttga 
tcaataaac tgacccagg attatttgt tgtaaatga 
ggacataca aggcgggcc ttgtatcca gtcacatcc 
agcaagtgt tcccacttg aagcattaa aataaaaat 
ctgatagtc acgatattg agaacaatg ctccttgtg 
gagacattt tttgtacgt gaccatacc ctgtaattc 
aattgttta accatgttg tggaccttg aaatgattg 
caagtatta aattggttt aagtacaaa gtatacagg 
tcacagcat aaataaagg aacaaaatt ggtgctgct 
gaaattttt gcaattaat tatgttgtg catttgcag 
cagtaacaa cagttgttt ttaagctgt attgatgga 
gttgttttc gtgtgaatt cgatgtcac ataccgtga 
tgtttaggt tttattttg tagtgtttg ttacaagtt 
ttttagctg atccatctt gtgaatcac aattgatgt 
tataagctc atatttgta ctatattat ttcgtttat 
tgcataagt atagtttgt atctggcaa ttaccaaat 
tatatgcat tttacttga cgaaaaata tatagcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY