Status
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Gene information
General information
TC008637 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13496583..13501377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008637
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08809

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008637 RA
      MFKGTVRIK IIQAEGLRP TDCSKRHNM MFGKSDDQM 
      DPYVTIDVD DTHLAQTTT KSKTLDPVW NESFEDDVV 
      DARTLTLTV FHDAAIPPN VFVANCSIP LEDLLQDHD 
      KDFWVNLEP TGRLRLQVD LERRGDIDT RRQFKERQG 
      FNRRRGAMR RRVHQVNGH KFMATYLRQ PTFCSHCRD 
      FIWGLMGKQ AYQCQVCAC VVHKRCHSL VLTKCPGIK 
      NETTQPNTG FSVNIPHRF VVHNYKRFT FCDHCGSLL 
      YGLFRQGLQ CEACSLNVH KRCQKNVAN NCGINVKQM 
      AEVLSSIGV SPDKQFQTH VKYVPATTN QEASADNTE 
      PNKTSNTTK ESNEEPQAC EDVKEQRSP NKIGLDDFN 
      FIKVLGKGS FGKVMLAER KDTEEIFAI KILKKDVII 
      QDDDVECTM TEKRILALA AKHPFLTAL HSCFQTKNR 
      LFFVMEYVN GGDLMFQIQ RARKFDEPR ARFYAAEVT 
      LALQFLHKH GVIYRDLKL DNILLDAEG HCKLADFGM 
      CKEGILEGI TTTTFCGTP DYIAPEILQ ELEYGASVD 
      WWALGVLMY EMMAGQPPF EADNEEDMY ESILHDDVL 
      YPVWLSKEA VNVLKGFMT KNPNKRLGC VVSQGGEAA 
      IKLHPFFKE IDWNALEQK KVKPPFKPR IKSKKDALN 
      FDAEFTKEE PILSPVSTE VTNSINQEE FKGFSFINP 
      NYNPNRFTS SD
    CDS sequence
    • >TC008637 RA
      atgtttaag ggcacagtg cggataaaa ataatccag 
      gccgaaggg ttgcgccct accgactgc tcgaaacgc 
      cacaacatg atgttcggc aagtctgac gaccagatg 
      gacccctac gtcaccatc gacgtggat gacacccac 
      ctggcccag accaccacc aagtcgaag acgctcgac 
      cccgtctgg aacgagtcc ttcgaggac gacgtcgtc 
      gacgccagg acgctcacg ttgaccgtt ttccacgac 
      gccgccata ccgcccaat gtctttgtc gccaactgt 
      agtataccg ctagaggat ttattacag gaccacgac 
      aaagatttt tgggttaat ttggagcct acaggccgc 
      ctccgcctg caagtggat ttagaacgt cgaggagat 
      attgatacg agacgccag ttcaaagag cgtcaaggc 
      ttcaaccga agacgcggt gcaatgcgt cgaagagtc 
      caccaagtc aatgggcac aaattcatg gctacgtat 
      ctccgccaa cccacattt tgttcccat tgtagagac 
      tttatttgg ggtttgatg ggcaaacaa gcctatcag 
      tgccaagtc tgtgcttgc gtcgttcac aaaagatgc 
      cactctttg gttctcacg aaatgtcca gggatcaaa 
      aatgagacc actcagcca aacacagga ttcagtgtg 
      aatattccc caccgattt gtggtccat aattacaaa 
      agattcacg ttttgtgac cattgcggg tctttgctg 
      tatggactt ttccggcaa ggactgcag tgtgaagcc 
      tgttctttg aacgttcac aagcggtgc cagaaaaat 
      gtagctaat aattgcgga ataaatgtt aaacaaatg 
      gccgaagtt ttgtcatcc attggtgtt tcccccgac 
      aaacaattc caaactcac gttaaatac gtaccggcc 
      accacaaac caagaggct tcagcggat aataccgaa 
      cccaacaaa acgagcaac accaccaag gaaagcaat 
      gaagaacca caggcgtgt gaagacgtc aaggaacaa 
      agaagtcca aataaaatc ggtctcgac gatttcaat 
      tttattaaa gttctgggg aagggaagt ttcgggaag 
      gtaatgcta gccgagagg aaggacacg gaggaaatc 
      ttcgcgatc aaaatcctc aagaaagat gtgattatc 
      caggacgac gacgtcgag tgcaccatg actgagaag 
      aggattctg gcgttagca gccaagcat ccgtttctg 
      accgcgctc cattcgtgt ttccaaacc aaaaaccgc 
      cttttcttc gtcatggag tacgtaaat gggggagat 
      ttgatgttt cagatccaa agggcgcgc aaattcgac 
      gagccaagg gcgaggttt tacgcagct gaggtcacg 
      ctagccctc cagttcttg cacaagcac ggggtcatt 
      tacagggac ttgaaactg gacaacatc ttgctggat 
      gccgagggg cactgcaag ttggccgac tttggcatg 
      tgcaaagag gggattctg gagggtatt acgaccact 
      acgttttgt ggcactccg gactacatc gctccggag 
      attttgcaa gagctggag tacggggcg agcgtggac 
      tggtgggct ttgggggtt ttgatgtac gagatgatg 
      gcgggacag ccgccgttt gaggccgat aacgaggag 
      gacatgtac gagtcgatc ttgcacgac gacgtgctt 
      tacccggtt tggctgagc aaggaggcg gttaatgtc 
      ctgaagggg ttcatgacg aagaacccc aacaagagg 
      ctggggtgc gtggtctcg caagggggc gaagcggcg 
      attaaattg cacccgttc ttcaaagag attgactgg 
      aacgcgttg gagcagaag aaagtaaaa ccgcctttt 
      aaacctcga attaaaagc aaaaaggat gcgctgaac 
      ttcgatgca gagtttacg aaagaagag ccaatcttg 
      agtccagtt agcaccgaa gtgacgaat tcaataaac 
      caagaggag ttcaaaggg ttttcgttt atcaatcca 
      aattacaat cccaatcgt tttacttca agcgactaa
    mRNA sequence
    • >TC008637 RA
      cattgtgaa tgtaaaacg gggaattgt gggagtcga 
      cgggatcgg gatagtcgg ctgtcaatc tccagagct 
      tgtgcgttc gtgtatttc gccgaaaaa ccgctacat 
      atccttcta tggtttcgc aaatcgtgt tttagtgcg 
      agtgcgatg tttaagggc acagtgcgg ataaaaata 
      atccaggcc gaagggttg cgccctacc gactgctcg 
      aaacgccac aacatgatg ttcggcaag tctgacgac 
      cagatggac ccctacgtc accatcgac gtggatgac 
      acccacctg gcccagacc accaccaag tcgaagacg 
      ctcgacccc gtctggaac gagtccttc gaggacgac 
      gtcgtcgac gccaggacg ctcacgttg accgttttc 
      cacgacgcc gccataccg cccaatgtc tttgtcgcc 
      aactgtagt ataccgcta gaggattta ttacaggac 
      cacgacaaa gatttttgg gttaatttg gagcctaca 
      ggccgcctc cgcctgcaa gtggattta gaacgtcga 
      ggagatatt gatacgaga cgccagttc aaagagcgt 
      caaggcttc aaccgaaga cgcggtgca atgcgtcga 
      agagtccac caagtcaat gggcacaaa ttcatggct 
      acgtatctc cgccaaccc acattttgt tcccattgt 
      agagacttt atttggggt ttgatgggc aaacaagcc 
      tatcagtgc caagtctgt gcttgcgtc gttcacaaa 
      agatgccac tctttggtt ctcacgaaa tgtccaggg 
      atcaaaaat gagaccact cagccaaac acaggattc 
      agtgtgaat attccccac cgatttgtg gtccataat 
      tacaaaaga ttcacgttt tgtgaccat tgcgggtct 
      ttgctgtat ggacttttc cggcaagga ctgcagtgt 
      gaagcctgt tctttgaac gttcacaag cggtgccag 
      aaaaatgta gctaataat tgcggaata aatgttaaa 
      caaatggcc gaagttttg tcatccatt ggtgtttcc 
      cccgacaaa caattccaa actcacgtt aaatacgta 
      ccggccacc acaaaccaa gaggcttca gcggataat 
      accgaaccc aacaaaacg agcaacacc accaaggaa 
      agcaatgaa gaaccacag gcgtgtgaa gacgtcaag 
      gaacaaaga agtccaaat aaaatcggt ctcgacgat 
      ttcaatttt attaaagtt ctggggaag ggaagtttc 
      gggaaggta atgctagcc gagaggaag gacacggag 
      gaaatcttc gcgatcaaa atcctcaag aaagatgtg 
      attatccag gacgacgac gtcgagtgc accatgact 
      gagaagagg attctggcg ttagcagcc aagcatccg 
      tttctgacc gcgctccat tcgtgtttc caaaccaaa 
      aaccgcctt ttcttcgtc atggagtac gtaaatggg 
      ggagatttg atgtttcag atccaaagg gcgcgcaaa 
      ttcgacgag ccaagggcg aggttttac gcagctgag 
      gtcacgcta gccctccag ttcttgcac aagcacggg 
      gtcatttac agggacttg aaactggac aacatcttg 
      ctggatgcc gaggggcac tgcaagttg gccgacttt 
      ggcatgtgc aaagagggg attctggag ggtattacg 
      accactacg ttttgtggc actccggac tacatcgct 
      ccggagatt ttgcaagag ctggagtac ggggcgagc 
      gtggactgg tgggctttg ggggttttg atgtacgag 
      atgatggcg ggacagccg ccgtttgag gccgataac 
      gaggaggac atgtacgag tcgatcttg cacgacgac 
      gtgctttac ccggtttgg ctgagcaag gaggcggtt 
      aatgtcctg aaggggttc atgacgaag aaccccaac 
      aagaggctg gggtgcgtg gtctcgcaa gggggcgaa 
      gcggcgatt aaattgcac ccgttcttc aaagagatt 
      gactggaac gcgttggag cagaagaaa gtaaaaccg 
      ccttttaaa cctcgaatt aaaagcaaa aaggatgcg 
      ctgaacttc gatgcagag tttacgaaa gaagagcca 
      atcttgagt ccagttagc accgaagtg acgaattca 
      ataaaccaa gaggagttc aaagggttt tcgtttatc 
      aatccaaat tacaatccc aatcgtttt acttcaagc 
      gactaatta ctgactaga gatgacttc agttaaatt 
      tcatccgta tgagtaact ttcaatcta actcagtta 
      atagataga agatttctg tgatcttac tggataatt 
      aagccagaa attaaatgt atatttacg aaaggccta 
      agctctgta aatattttg gtgaattga gaagtttta 
      ttagatcga gtcaattta ttgttgtgg tagccttaa 
      aattgcaca agactcatg ttttcacat tttgtgttt 
      gcaaccagg agaacgtaa agagcttat cgagagaga 
      tttatagcg accattgtt gataatata atagtttat 
      gtagatttt gtttaaagt gtggcacaa actgatgta 
      aagaatgcc acacaatta ccatgaacg tggagagca 
      atcattacg caaagtgtg cctgttgat gttgtatga 
      gttaaataa atttatcta ccattgagc tgtttagaa 
      caaatcgga cacatttca aggaaatgt ttgttgttg 
      gaaaggaca aaaatgttt acgtttatg tctgtagtc 
      gtaatatta ttagtacag ccagctaat atagtgatg 
      gatgtttag cgagtttcc catcataag atacagatt 
      tttaaccca ttcgatgat ttgtttttt actattctt 
      cacgacgtt gtcaaataa ctttgcgtc cgttatcgt 
      gccaaattt attttgcct acctcatct atttttatg 
      acaaatacc ataatgtta tcgtttctg ttgtgttct 
      tagtacaat gtgtaaatt tattaggtg gtattgtac 
      tattcctcc aaaacagtg taccaatta tagtagata 
      tgttaatgc aattcttca aatgtaact tgttttatt 
      tccttccgg acttcttta tgagtttca cagccaagc 
      acaccttat ttttatggg caaaatgac ggatacctg 
      tacttacaa atttattta gtcagtacc ataaccggt 
      attagcata tctacttaa aatgaaata aatattatt 
      tagtgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY