Status
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Gene information
General information
TC008635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13471303..13477517
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008635
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08808

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008635 RA
      MSEKPNKNV SGAGASAPN KMRLYATWV DRTPSNCIP 
      RLCSLTLTR LVVLKPLGS DLASISIAV KMQSSKRTL 
      RSNELILPP NGLLDTPLE LTFCLQYPH FLKREGNKL 
      HILLQRRKR YKNRTMLGF KTLAEGIIR MDQVLQRQM 
      DMELELHPE GGGKDKEKG PVARLSVLQ LSSTPVDQE 
      HKTDRDHDF SDDDDEISS GEEEVADLS DSEPIRTKL 
      PHTRHNLKQ RFVSLLRRF KVPDSEGGR SADLSNPSD 
      IQALFQELE SLSCDEDSA GEQDTMSIS STPKPSLRP 
      FFSSSKSLL DSNQTHYSE TEKFIDDKA SGSDGNADI 
      FTDQEAQSD PQTGSPPRE QTATLRAKL SGDNDFANL 
      MQELNERKS KLFRSSASS GKKKNSLSV SSDAPVPET 
      VTARKIFLE QVTRVLPLE ENALPEAVV LITGPDSIT 
      GPLSARLTH HRIFLPLSS VEVKAVLTA VFNKIHKYC 
      NSCPKPGSF VKLILIGGD TLLGWVIRP YVELLSSKP 
      PEWLNYTRI YIIPVGSCG ITKHLGNLD QGYASLFPS 
      DQELKIDEL AGRLQRYLS VPSSAPVAQ LPLGEAMLT 
      CQDDSSQLF VPFVNEVRV GPAESALSV SVDLEDLMC 
      SSPPAPSLT PPSSPNVQT RDSPWEPLE LQLDYWQSP 
      KSTEPQAAK TDKTKQDGK TSLKGLFRG LQASPGLNV 
      TMHLANKEK KQKIMRLGK KKEKEKDAE PRSQTVEGI 
      SRLICSAKA SHNTPMKVY VDGVEFNGV KFFQLSSTW 
      QTHVKHLSV ALVGVPLAS AEVNSM
    CDS sequence
    • >TC008635 RA
      atgagcgaa aaaccgaat aaaaacgtg agcggggcg 
      ggtgcctcc gcgcccaac aaaatgagg ctttatgcg 
      acttgggtc gaccggact ccctcgaac tgtattccg 
      aggctttgt tcattgacg ctgacaagg ctggttgtt 
      ttaaagcct ttaggatca gatctagcc tctatttct 
      attgccgtt aaaatgcag tcgagcaaa cgcacatta 
      cgctccaat gagctgatt ttgccaccg aacggtcta 
      ttagacacg ccgttggaa ttaactttt tgtctccaa 
      tatccacat tttttgaaa cgggagggc aacaagctg 
      catattttg ctccagagg cggaaacgg tacaagaat 
      cgcacaatg ctcgggttc aaaacacta gcagaaggg 
      attatcaga atggaccaa gttctgcaa aggcaaatg 
      gacatggag ctggaactg cacccggaa gggggcggc 
      aaagacaaa gagaaggga cccgtggcc cgcttgagc 
      gtcctgcaa ctgagctcg accccggtc gaccaagag 
      cacaaaacg gaccgagac cacgacttc agcgacgac 
      gacgacgaa atcagctca ggcgaggaa gaagttgcc 
      gatttatcc gacagcgaa ccgatccga accaaactc 
      ccacacact cgtcacaat ctcaagcaa cgtttcgtc 
      tctctgttg cgccgcttc aaggtgccc gacagcgag 
      ggcggtcga agcgccgac ttgtccaac cctagcgac 
      atccaagct ctctttcaa gaactcgaa tcgctgagt 
      tgcgacgaa gactcggcc ggcgaacaa gacaccatg 
      tccatatcg agcacgcca aagccgagc cttcggccg 
      tttttcagc agtagcaag agtttgtta gactcgaat 
      cagacgcat tacagcgaa accgagaaa tttatcgac 
      gacaaagcg tccggaagc gacggaaat gcggatatt 
      ttcacggat caggaagcg cagtcggat ccacagacc 
      ggctcgccg cccagagag caaacggcg acgcttcga 
      gcgaagttg tcgggggat aacgatttt gcgaatttg 
      atgcaagag ttgaatgag aggaagtcg aagttgttt 
      aggtcgtcg gcgtcttcc ggtaaaaag aagaactcg 
      ttgagtgtc agttccgac gctccggtg cccgaaact 
      gttacggcg cggaaaatt tttctcgag caagtcaca 
      cgagttctt cccctggaa gaaaacgct ctcccggaa 
      gctgtggta ttaattaca ggccctgac agtattact 
      ggaccatta tcagcccgt ctcacgcat catcggatt 
      ttccttcca ttatcatca gtcgaagtt aaagcagtc 
      ctgactgcg gttttcaac aaaattcac aaatattgc 
      aatagctgt cccaaaccg ggcagcttt gttaaatta 
      atcctgatt ggtggagac actctcctc ggttgggtc 
      atccgacct tacgtcgaa cttttatca agtaagccg 
      ccggaatgg ttgaattac acaagaatt tacataatt 
      ccggtcggt tcttgcggg attacaaaa cacctcggt 
      aacttagac cagggttat gcctcttta ttcccctca 
      gaccaggaa ttaaaaatc gacgaattg gccgggcgg 
      ctacaacgg tacttatca gtaccatca tcggctccg 
      gttgcgcag cttcctctc ggggaggcg atgctcacc 
      tgtcaggac gactccagt caactcttc gtcccgttt 
      gttaacgag gtgcgtgtg ggtcccgcc gaatcagca 
      ttatccgta tctgtagat cttgaagat ttgatgtgt 
      tccagtccc cctgccccc tccctaact cccccatcg 
      tcccccaac gtgcaaacg cgcgactcg ccctgggag 
      cccctcgaa ttgcaatta gactattgg caaagcccc 
      aaaagtact gaacctcaa gccgcaaag actgacaaa 
      accaaacaa gacggtaaa acttcactc aaaggactg 
      ttccggggc cttcaagcc tcgccgggg ctcaacgtc 
      accatgcac ttggcgaat aaggagaaa aaacagaaa 
      attatgaga ctgggtaag aagaaggag aaggagaag 
      gatgccgag cctcgcagt cagactgtg gagggaatt 
      tcgaggttg atttgttcc gcgaaagcg tcccacaac 
      acgccgatg aaagtttat gtggacggc gtggagttt 
      aacggagtt aaattcttc cagttgagt tcgacttgg 
      caaacgcac gttaaacac ttgtcggtg gctctggta 
      ggcgtgcca ttggcgagt gccgaagtg aattcgatg 
      taa
    mRNA sequence
    • >TC008635 RA
      gttgcatcg caactgtca ctttgcggt ctttgtact 
      cgtttgaca gttcgtgat gcagttgac ataaaccgt 
      gcaaaaaat acgtgtagt gatgatcgt gggcaatgt 
      tggagcggc cggggctgt ccccggatc tagcacttg 
      agcgcacgg ggcccctgc tttagtcga ctacgcccc 
      cgcaagaat gagcgaaaa accgaataa aaacgtgag 
      cggggcggg tgcctccgc gcccaacaa aatgaggct 
      ttatgcgac ttgggtcga ccggactcc ctcgaactg 
      tattccgag gctttgttc attgacgct gacaaggct 
      ggttgtttt aaagccttt aggatcaga tctagcctc 
      tatttctat tgccgttaa aatgcagtc gagcaaacg 
      cacattacg ctccaatga gctgatttt gccaccgaa 
      cggtctatt agacacgcc gttggaatt aactttttg 
      tctccaata tccacattt tttgaaacg ggagggcaa 
      caagctgca tattttgct ccagaggcg gaaacggta 
      caagaatcg cacaatgct cgggttcaa aacactagc 
      agaagggat tatcagaat ggaccaagt tctgcaaag 
      gcaaatgga catggagct ggaactgca cccggaagg 
      gggcggcaa agacaaaga gaagggacc cgtggcccg 
      cttgagcgt cctgcaact gagctcgac cccggtcga 
      ccaagagca caaaacgga ccgagacca cgacttcag 
      cgacgacga cgacgaaat cagctcagg cgaggaaga 
      agttgccga tttatccga cagcgaacc gatccgaac 
      caaactccc acacactcg tcacaatct caagcaacg 
      tttcgtctc tctgttgcg ccgcttcaa ggtgcccga 
      cagcgaggg cggtcgaag cgccgactt gtccaaccc 
      tagcgacat ccaagctct ctttcaaga actcgaatc 
      gctgagttg cgacgaaga ctcggccgg cgaacaaga 
      caccatgtc catatcgag cacgccaaa gccgagcct 
      tcggccgtt tttcagcag tagcaagag tttgttaga 
      ctcgaatca gacgcatta cagcgaaac cgagaaatt 
      tatcgacga caaagcgtc cggaagcga cggaaatgc 
      ggatatttt cacggatca ggaagcgca gtcggatcc 
      acagaccgg ctcgccgcc cagagagca aacggcgac 
      gcttcgagc gaagttgtc gggggataa cgattttgc 
      gaatttgat gcaagagtt gaatgagag gaagtcgaa 
      gttgtttag gtcgtcggc gtcttccgg taaaaagaa 
      gaactcgtt gagtgtcag ttccgacgc tccggtgcc 
      cgaaactgt tacggcgcg gaaaatttt tctcgagca 
      agtcacacg agttcttcc cctggaaga aaacgctct 
      cccggaagc tgtggtatt aattacagg ccctgacag 
      tattactgg accattatc agcccgtct cacgcatca 
      tcggatttt ccttccatt atcatcagt cgaagttaa 
      agcagtcct gactgcggt tttcaacaa aattcacaa 
      atattgcaa tagctgtcc caaaccggg cagctttgt 
      taaattaat cctgattgg tggagacac tctcctcgg 
      ttgggtcat ccgacctta cgtcgaact tttatcaag 
      taagccgcc ggaatggtt gaattacac aagaattta 
      cataattcc ggtcggttc ttgcgggat tacaaaaca 
      cctcggtaa cttagacca gggttatgc ctctttatt 
      cccctcaga ccaggaatt aaaaatcga cgaattggc 
      cgggcggct acaacggta cttatcagt accatcatc 
      ggctccggt tgcgcagct tcctctcgg ggaggcgat 
      gctcacctg tcaggacga ctccagtca actcttcgt 
      cccgtttgt taacgaggt gcgtgtggg tcccgccga 
      atcagcatt atccgtatc tgtagatct tgaagattt 
      gatgtgttc cagtccccc tgccccctc cctaactcc 
      cccatcgtc ccccaacgt gcaaacgcg cgactcgcc 
      ctgggagcc cctcgaatt gcaattaga ctattggca 
      aagccccaa aagtactga acctcaagc cgcaaagac 
      tgacaaaac caaacaaga cggtaaaac ttcactcaa 
      aggactgtt ccggggcct tcaagcctc gccggggct 
      caacgtcac catgcactt ggcgaataa ggagaaaaa 
      acagaaaat tatgagact gggtaagaa gaaggagaa 
      ggagaagga tgccgagcc tcgcagtca gactgtgga 
      gggaatttc gaggttgat ttgttccgc gaaagcgtc 
      ccacaacac gccgatgaa agtttatgt ggacggcgt 
      ggagtttaa cggagttaa attcttcca gttgagttc 
      gacttggca aacgcacgt taaacactt gtcggtggc 
      tctggtagg cgtgccatt ggcgagtgc cgaagtgaa 
      ttcgatgta acataagac gaccttccc gttttttaa 
      atccgccgc tttgcttcc gtgtctcgt tcccgcgat 
      gcattcatc gcttctagt caaggtcaa atccatcgt 
      aatccaacc gttgacaat acatccaaa taaggctca 
      aaactcttg tttgaaaaa aagagagga aactcacgt 
      taagaatac tccgtatat gtgatatag tttatagtc 
      gtcgttatc gataatgct tgagatttt ttcagaaaa 
      aatcaatac ttttttaca tgctaagat ttaatcatt 
      tttaataag cacagcaag atttttttg tggccaaac 
      cacttttat atgatataa cgtattcgg gttttacac 
      accattgga acgcgttaa catgccgcc cactttaag 
      cttagggga agtggtgtg caagacctc catatcagc 
      attttgcat aaataccaa agcgagaat ttgtgttat 
      ttgacggaa agttgttta tattaatgt gtaggtttc 
      tggcagtca tttactccg tggagtaat tgctgggaa 
      tagttccat gtcaaatca aatcttgtt gggaatgtt 
      gaaaggatc tcacgttga ttgttgaac tgtgatgtg 
      tgaacatag gaaattgat ataaatgaa aacgccgtg 
      cgactgtga ttttaacgc agctcattc caacttgtt 
      agttagatg ggtgttctc tcattttat ctcatttgg 
      aatgtgcaa caagtttta gaacatcca tctttagga 
      catcagtgt ttgattgta aatgataga ttagattat 
      ctgttgtat aaatgcaat aatgtctgg aattagtat 
      tgtttatat ataactgca tacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY