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Gene information
General information
TC033835 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13129773..13131557
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033835
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08805

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033835 RA
      MAHPLSERH RYFIQFMLT NGLVTVDKA 
      LKFFGELSP DQNQLVHLK SLIIEINRA 
      INLQYFKIA TSVCEVTGD SVFVWLNTK 
      NDELSKQQI GFSPVELEY FHAIMQEII 
      TSDNHELPY PRCINITST LVGTLTREN 
      GEKALMKWI RGGYFVKVE QFVYLGART 
      ILEFTTYLR TNTENCTCS LCSELVFRG 
      ESCQSCFNT FHTFCIQKY LQNQHRCPS 
      CRTDWEGAN TNTSMDVDS EEGAG
    CDS sequence
    • >TC033835 RA
      atggcccac cccctgagc gaaagacat cgatatttt 
      atacagttt atgttgact aacggttta gtaaccgta 
      gataaggct ttaaagttt tttggcgag cttagcccc 
      gatcaaaac cagttagtt catttaaaa tcgttgatc 
      attgagatc aacagagca ataaacctg cagtatttt 
      aaaatagcg acttcggtc tgtgaggta acaggtgac 
      agtgttttc gtctggctg aatacaaaa aatgacgag 
      ttgagtaaa caacagata ggattttca ccggtggaa 
      ttggaatat ttccacgcg ataatgcaa gaaattatc 
      acctcagac aaccacgaa ttgccgtat cctcgctgt 
      atcaatata acctcaact ttggtggga actttgact 
      cgcgaaaat ggggaaaag gcgttaatg aaatggatt 
      agaggggga tatttcgta aaagtggag cagtttgtg 
      tatctcggc gctaggact attttagaa ttcacaacg 
      tacttgagg actaataca gaaaactgt acttgcagc 
      ctgtgttca gagctggtt tttaggggc gaaagttgc 
      cagtcgtgt ttcaacaca ttccataca ttttgcatc 
      caaaaatat ctccaaaac cagcatagg tgcccctct 
      tgtagaacc gactgggaa ggagcaaac actaacact 
      agtatggat gtagattct gaagaaggt gcaggatag
    mRNA sequence
    • >TC033835 RA
      gtgccggcc acatttcac gctcaatta aatcgccag 
      gcttagtag caagcgagg tggtgttaa gccagtgca 
      tgcaaaata atacccaaa aagaggttg taagcagtg 
      ggctgtcga catcctcaa taagaacag aaggaataa 
      tctgctccc cgaacgtct taatttgct cgcaattca 
      tggaaacca aagaaacga ctaaataaa aattgtgcg 
      gttgtacca atcctagca acgcttggt tggaaaatt 
      cgcatgaac tcaccgagc caacgtaga actgagtgg 
      gtcttgccg ctttgcgaa gaccttgat aaccatgct 
      ccagttcag tgtaagaca tggtttaac tcaaacatt 
      ccttcggag tttggctgg gactggacg gccattcac 
      gaatttgca atgcctttt gcttctgca agggcttcc 
      tcaccggtt tgttcaatg ctttgggca ggtcaagaa 
      gtattgatt ggcctagat actgcctta tgcgatatt 
      tgcgccgaa atcaccaat cagttacgt aaatgttct 
      caaaataaa cgtttttta agctgaacc catcatcgt 
      cacgaaatt attcatcaa aaaggatat tcatagaac 
      ttccaaaac acagcattt gacaactcg atgacagac 
      gtttgacaa ttgagaatt gacagcggt caaaatttt 
      ggaaaaatc taacaaata attgttatt tcaaaatta 
      ccagctata agattcgtt tttaataaa ttggttaat 
      tttcactaa acattggac tgaatgttt aacattatt 
      gcatatttg gcgttttgg cgccctttt ggatcctga 
      caagtgagg ttgtgtttg tgtctggaa attttcaaa 
      ttttgcgaa atggcccac cccctgagc gaaagacat 
      cgatatttt atacagttt atgttgact aacggttta 
      gtaaccgta gataaggct ttaaagttt tttggcgag 
      cttagcccc gatcaaaac cagttagtt catttaaaa 
      tcgttgatc attgagatc aacagagca ataaacctg 
      cagtatttt aaaatagcg acttcggtc tgtgaggta 
      acaggtgac agtgttttc gtctggctg aatacaaaa 
      aatgacgag ttgagtaaa caacagata ggattttca 
      ccggtggaa ttggaatat ttccacgcg ataatgcaa 
      gaaattatc acctcagac aaccacgaa ttgccgtat 
      cctcgctgt atcaatata acctcaact ttggtggga 
      actttgact cgcgaaaat ggggaaaag gcgttaatg 
      aaatggatt agaggggga tatttcgta aaagtggag 
      cagtttgtg tatctcggc gctaggact attttagaa 
      ttcacaacg tacttgagg actaataca gaaaactgt 
      acttgcagc ctgtgttca gagctggtt tttaggggc 
      gaaagttgc cagtcgtgt ttcaacaca ttccataca 
      ttttgcatc caaaaatat ctccaaaac cagcatagg 
      tgcccctct tgtagaacc gactgggaa ggagcaaac 
      actaacact agtatggat gtagattct gaagaaggt 
      gcaggatag gctaggaga aatgattaa gagtcgtga 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY