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Gene information
General information
TC008758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9593647..9596822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008758
    iBeetle screen iB_08800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008758 RA
      MTTQAEQLN NKRLNLEKA TNFMRQYRD PHSRELKKL 
SANQFMEVW SHYDKDGNG YIEGTELDG FLREFVSSA 
NATDVGPEA VSDDMLVEL KACFMEAYD DNQDGKIDI 
RELAQLLPM EENFLLLFR FDNPLESSV EFMKIWREY 
DTDGSGYIE ADELKNFLR DLLKEAKKI NDVSEDKLI 
EYTDTMLQV FDANKDGRL QLSEMAKLL PVKENFLCR 
QIFKGATKL TKDDIERVF ALYDRDNNG TIENEELRG 
FLKDLLELV KKDYDAQDL LDFEETILR GVDYNQDGK 
INKKELTMI LLAIAKHNQ EEESTA
    CDS sequence
    • >TC008758 RA
      atgacgacc caagcggag cagctcaac aataaacgt 
ctcaacttg gaaaaagca acaaacttc atgaggcaa 
tatcgcgac ccccattcc cgcgaactg aaaaaactt 
tcggcgaat cagttcatg gaagtctgg agtcactac 
gacaaagac gggaatgga tatatcgag ggaacggaa 
ctggacggc tttttgagg gagttcgtg tcaagtgcc 
aatgccacg gatgtcggg ccggaagcg gtctccgac 
gacatgttg gtggagctg aaagcgtgc ttcatggag 
gcttacgat gacaaccag gatggaaaa atcgatatt 
cgtgagttg gcgcagtta cttccgatg gaagaaaac 
ttcttactc cttttccga ttcgacaat cccttagag 
tccagtgtg gagtttatg aagatttgg cgcgagtac 
gacaccgat ggtagcggt tacatcgaa gccgacgag 
ctcaaaaat ttcctgcga gacttgctg aaagaagcg 
aaaaagatt aacgacgtt tccgaagat aaactcatc 
gaatacacc gacaccatg ctccaggtg tttgacgcc 
aacaaggac ggccgtttg caactgtcg gaaatggcc 
aagttactt cctgttaaa gaaaatttt ctctgcagg 
caaatcttc aagggggct acaaaactc accaaggac 
gatatcgag cgagttttt gccttgtac gacagggat 
aacaacggt acaatagaa aacgaagag ttgcgcggc 
tttttgaaa gatttgctc gaactggtc aaaaaagac 
tacgatgct caagatttg ctcgatttt gaggaaacc 
attctacga ggcgtggac tacaatcaa gatggcaaa 
atcaacaag aaggaattg acgatgatc ttgttagcc 
atcgcgaaa cataatcaa gaggaggaa tcaaccgca 
tag
    mRNA sequence
    • >TC008758 RA
      gttgcagat tcgcggaga tgacgaccc aagcggagc 
agctcaaca ataaacgtc tcaacttgg aaaaagcaa 
caaacttca tgaggcaat atcgcgacc cccattccc 
gcgaactga aaaaacttt cggcgaatc agttcatgg 
aagtctgga gtcactacg acaaagacg ggaatggat 
atatcgagg gaacggaac tggacggct ttttgaggg 
agttcgtgt caagtgcca atgccacgg atgtcgggc 
cggaagcgg tctccgacg acatgttgg tggagctga 
aagcgtgct tcatggagg cttacgatg acaaccagg 
atggaaaaa tcgatattc gtgagttgg cgcagttac 
ttccgatgg aagaaaact tcttactcc ttttccgat 
tcgacaatc ccttagagt ccagtgtgg agtttatga 
agatttggc gcgagtacg acaccgatg gtagcggtt 
acatcgaag ccgacgagc tcaaaaatt tcctgcgag 
acttgctga aagaagcga aaaagatta acgacgttt 
ccgaagata aactcatcg aatacaccg acaccatgc 
tccaggtgt ttgacgcca acaaggacg gccgtttgc 
aactgtcgg aaatggcca agttacttc ctgttaaag 
aaaattttc tctgcaggc aaatcttca agggggcta 
caaaactca ccaaggacg atatcgagc gagtttttg 
ccttgtacg acagggata acaacggta caatagaaa 
acgaagagt tgcgcggct ttttgaaag atttgctcg 
aactggtca aaaaagact acgatgctc aagatttgc 
tcgattttg aggaaacca ttctacgag gcgtggact 
acaatcaag atggcaaaa tcaacaaga aggaattga 
cgatgatct tgttagcca tcgcgaaac ataatcaag 
aggaggaat caaccgcat agatatcat gaggaagga 
aatcgtaac gagttgcca atttatcaa gtagcacaa 
gtttcagga gtttataag taattatat atagggtta 
aatttaatg ttagattat tgtgacacc aatattgtt 
gttgatttg gtagcgtga ctacaataa ggtaaaaac 
gtgcatcaa tctctcaat tataatcat ttttacgat 
caattttcg catcgctgt gattttatt ggaagtact 
cgtaagtag agggcaaca cggttgata tttttataa 
tgcaatagc cttgagatc agattaagt tactttcag 
ttgatattc caaatgagg gagaagaaa caccaatcc 
aaaccaaag acttatttg atacaaaac ccaatccta 
agaatgcac aactttcat atcgcactt caatcaact 
gttactgaa ttatactta gactagtca tgcatgatc 
tctgagctt tcggtactc aaatatttc aaagtttac 
aagtagtga tgttgctct atttatcta taactatat 
aactatatt tataaacgc tgctacggc actgtatta 
tcgattgtg atacctttc attgaacga aatgaaaga 
cttgggaga atcgaattt tattgattg gtctcgaag 
cctaagtag atgaaaacg tggtacatt atcaaaccg 
cagcttcga atattatta tcttgaaaa ttcgtccag 
tttcgcata tctgtgccc ctctatgtg cattattgt 
ataataaag tctatttta ataaaaagt atagattaa 
tttatatat gttaacact tttatattt attacgtta 
tgtacaaag ttgccaaat tctcttaga cgtgtgaaa 
tacttacag ttataatac tataaggcg atttttgct 
tgtttttcg ttcaattca gtgtgataa aacattttc 
aaactacta cgaaaacgg ttcactatg tcctactat 
actgtaaaa aataaatgt tcgttattt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY