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Gene information
General information
TC008758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9593647..9596822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008758
    iBeetle screen iB_08800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008758 RA
      MTTQAEQLN NKRLNLEKA TNFMRQYRD PHSRELKKL 
      SANQFMEVW SHYDKDGNG YIEGTELDG FLREFVSSA 
      NATDVGPEA VSDDMLVEL KACFMEAYD DNQDGKIDI 
      RELAQLLPM EENFLLLFR FDNPLESSV EFMKIWREY 
      DTDGSGYIE ADELKNFLR DLLKEAKKI NDVSEDKLI 
      EYTDTMLQV FDANKDGRL QLSEMAKLL PVKENFLCR 
      QIFKGATKL TKDDIERVF ALYDRDNNG TIENEELRG 
      FLKDLLELV KKDYDAQDL LDFEETILR GVDYNQDGK 
      INKKELTMI LLAIAKHNQ EEESTA
    CDS sequence
    • >TC008758 RA
      atgacgacc caagcggag cagctcaac aataaacgt 
      ctcaacttg gaaaaagca acaaacttc atgaggcaa 
      tatcgcgac ccccattcc cgcgaactg aaaaaactt 
      tcggcgaat cagttcatg gaagtctgg agtcactac 
      gacaaagac gggaatgga tatatcgag ggaacggaa 
      ctggacggc tttttgagg gagttcgtg tcaagtgcc 
      aatgccacg gatgtcggg ccggaagcg gtctccgac 
      gacatgttg gtggagctg aaagcgtgc ttcatggag 
      gcttacgat gacaaccag gatggaaaa atcgatatt 
      cgtgagttg gcgcagtta cttccgatg gaagaaaac 
      ttcttactc cttttccga ttcgacaat cccttagag 
      tccagtgtg gagtttatg aagatttgg cgcgagtac 
      gacaccgat ggtagcggt tacatcgaa gccgacgag 
      ctcaaaaat ttcctgcga gacttgctg aaagaagcg 
      aaaaagatt aacgacgtt tccgaagat aaactcatc 
      gaatacacc gacaccatg ctccaggtg tttgacgcc 
      aacaaggac ggccgtttg caactgtcg gaaatggcc 
      aagttactt cctgttaaa gaaaatttt ctctgcagg 
      caaatcttc aagggggct acaaaactc accaaggac 
      gatatcgag cgagttttt gccttgtac gacagggat 
      aacaacggt acaatagaa aacgaagag ttgcgcggc 
      tttttgaaa gatttgctc gaactggtc aaaaaagac 
      tacgatgct caagatttg ctcgatttt gaggaaacc 
      attctacga ggcgtggac tacaatcaa gatggcaaa 
      atcaacaag aaggaattg acgatgatc ttgttagcc 
      atcgcgaaa cataatcaa gaggaggaa tcaaccgca 
      tag
    mRNA sequence
    • >TC008758 RA
      gttgcagat tcgcggaga tgacgaccc aagcggagc 
      agctcaaca ataaacgtc tcaacttgg aaaaagcaa 
      caaacttca tgaggcaat atcgcgacc cccattccc 
      gcgaactga aaaaacttt cggcgaatc agttcatgg 
      aagtctgga gtcactacg acaaagacg ggaatggat 
      atatcgagg gaacggaac tggacggct ttttgaggg 
      agttcgtgt caagtgcca atgccacgg atgtcgggc 
      cggaagcgg tctccgacg acatgttgg tggagctga 
      aagcgtgct tcatggagg cttacgatg acaaccagg 
      atggaaaaa tcgatattc gtgagttgg cgcagttac 
      ttccgatgg aagaaaact tcttactcc ttttccgat 
      tcgacaatc ccttagagt ccagtgtgg agtttatga 
      agatttggc gcgagtacg acaccgatg gtagcggtt 
      acatcgaag ccgacgagc tcaaaaatt tcctgcgag 
      acttgctga aagaagcga aaaagatta acgacgttt 
      ccgaagata aactcatcg aatacaccg acaccatgc 
      tccaggtgt ttgacgcca acaaggacg gccgtttgc 
      aactgtcgg aaatggcca agttacttc ctgttaaag 
      aaaattttc tctgcaggc aaatcttca agggggcta 
      caaaactca ccaaggacg atatcgagc gagtttttg 
      ccttgtacg acagggata acaacggta caatagaaa 
      acgaagagt tgcgcggct ttttgaaag atttgctcg 
      aactggtca aaaaagact acgatgctc aagatttgc 
      tcgattttg aggaaacca ttctacgag gcgtggact 
      acaatcaag atggcaaaa tcaacaaga aggaattga 
      cgatgatct tgttagcca tcgcgaaac ataatcaag 
      aggaggaat caaccgcat agatatcat gaggaagga 
      aatcgtaac gagttgcca atttatcaa gtagcacaa 
      gtttcagga gtttataag taattatat atagggtta 
      aatttaatg ttagattat tgtgacacc aatattgtt 
      gttgatttg gtagcgtga ctacaataa ggtaaaaac 
      gtgcatcaa tctctcaat tataatcat ttttacgat 
      caattttcg catcgctgt gattttatt ggaagtact 
      cgtaagtag agggcaaca cggttgata tttttataa 
      tgcaatagc cttgagatc agattaagt tactttcag 
      ttgatattc caaatgagg gagaagaaa caccaatcc 
      aaaccaaag acttatttg atacaaaac ccaatccta 
      agaatgcac aactttcat atcgcactt caatcaact 
      gttactgaa ttatactta gactagtca tgcatgatc 
      tctgagctt tcggtactc aaatatttc aaagtttac 
      aagtagtga tgttgctct atttatcta taactatat 
      aactatatt tataaacgc tgctacggc actgtatta 
      tcgattgtg atacctttc attgaacga aatgaaaga 
      cttgggaga atcgaattt tattgattg gtctcgaag 
      cctaagtag atgaaaacg tggtacatt atcaaaccg 
      cagcttcga atattatta tcttgaaaa ttcgtccag 
      tttcgcata tctgtgccc ctctatgtg cattattgt 
      ataataaag tctatttta ataaaaagt atagattaa 
      tttatatat gttaacact tttatattt attacgtta 
      tgtacaaag ttgccaaat tctcttaga cgtgtgaaa 
      tacttacag ttataatac tataaggcg atttttgct 
      tgtttttcg ttcaattca gtgtgataa aacattttc 
      aaactacta cgaaaacgg ttcactatg tcctactat 
      actgtaaaa aataaatgt tcgttattt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY