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Gene information
General information
TC010058 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9667089..9675705
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010058
    iBeetle screen iB_08799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010058 RA
      MSLPSSGVD MSSLMSQHP VLGAIPPAF FLRASERYQ 
      RTPKCARCR NHGVVSALK GHKRYCRWR DCNCAKCTL 
      IAERQRVMA AQVALRRQQ AQEENEARE LGILFPTPA 
      GVVADTPGV TAPAIPQNS DVGISQLMQ RNTFTASDS 
      SEPSSPTSK RPRINVEDC SLEGSDSEP EDLKKSRQS 
      SPVPAPAPS APTPSPEPQ TSPDPDLDV EEDTQSEAP 
      ENLSLKKPS SPETPPQPT QNFIPYQQF AFPPFQPQY 
      PAQRSPIDV LMRVFPGKR RSDVEALLQ RCKGDVVQA 
      MEMMVSGSH EDATPPSAF SPLGPPTNF HRFSPSRRF 
      LSAPYAGTG YLPTVIRPP PDYLSMVGS VHDIYSSDK 
      TSASSPGSN TSSDKTSYS E
    CDS sequence
    • >TC010058 RA
      atgagcctt cccagcagc ggcgtcgac atgtcctcc 
      ctcatgtcg caacaccca gtcctgggt gctattccc 
      ccggctttt ttcttacgc gcctccgaa cgctaccaa 
      cgaactcca aaatgtgca cgttgtcgt aaccacggc 
      gtcgtgtcg gccctcaaa ggtcacaaa agatactgt 
      cgatggcgc gattgcaac tgcgccaaa tgcactctt 
      attgctgaa agacaaaga gttatggcg gcccaagta 
      gctctcaga agacagcaa gcacaagaa gaaaatgaa 
      gccagagag ttgggaatt ctgttcccg actcctgcc 
      ggcgttgta gcggacaca ccaggagtt actgctcct 
      gctatacca cagaattcc gacgtagga atctcacag 
      ttgatgcag aggaacacg ttcacagct tcggattcg 
      tcggaaccg tcgagtcca acctcgaag cggcccaga 
      atcaacgtc gaggactgc tccttggaa ggctccgac 
      tccgagccc gaagacttg aaaaagagc cgtcagtcg 
      agtccggtc cccgcgccg gctccctca gcccccacc 
      ccctccccc gaaccccaa acaagccca gatcccgac 
      ttggacgtg gaggaggac acccagagc gaagccccc 
      gagaatctc tccctcaag aagccatcc agtccggag 
      acacctccg caaccgaca cagaacttc atcccttac 
      cagcagttc gcatttccc ccctttcag ccgcagtac 
      cccgcccag cgctcgccc atcgacgtg ctcatgagg 
      gtgttcccg gggaagagg aggtcggac gtcgaggcc 
      cttttgcag aggtgcaaa ggcgacgtg gtgcaggcc 
      atggagatg atggtgagt gggagccac gaggacgcc 
      acgccgcca tcggcgttc agtcctctg ggcccccca 
      accaacttc caccggttc agcccttcg aggagattc 
      ctgtcggct ccgtacgcc gggacgggg tatctccca 
      acggtgata aggcctcct cctgattat ctgtcgatg 
      gtggggtcg gtgcatgat atttacagc agcgataag 
      acctcggcg tcttctccg ggctccaac acgagctcg 
      gacaagacg tcgtactcg gaatag
    mRNA sequence
    • >TC010058 RA
      cggaacttc gggtagtgg cgagttcgc gcgctgtgg 
      aatggtgaa ctgaacgac caaaactca cagaggacc 
      acacacaac accacacta ccatgagcc ttcccagca 
      gcggcgtcg acatgtcct ccctcatgt cgcaacacc 
      cagtcctgg gtgctattc ccccggctt ttttcttac 
      gcgcctccg aacgctacc aacgaactc caaaatgtg 
      cacgttgtc gtaaccacg gcgtcgtgt cggccctca 
      aaggtcaca aaagatact gtcgatggc gcgattgca 
      actgcgcca aatgcactc ttattgctg aaagacaaa 
      gagttatgg cggcccaag tagctctca gaagacagc 
      aagcacaag aagaaaatg aagccagag agttgggaa 
      ttctgttcc cgactcctg ccggcgttg tagcggaca 
      caccaggag ttactgctc ctgctatac cacagaatt 
      ccgacgtag gaatctcac agttgatgc agaggaaca 
      cgttcacag cttcggatt cgtcggaac cgtcgagtc 
      caacctcga agcggccca gaatcaacg tcgaggact 
      gctccttgg aaggctccg actccgagc ccgaagact 
      tgaaaaaga gccgtcagt cgagtccgg tccccgcgc 
      cggctccct cagccccca ccccctccc ccgaacccc 
      aaacaagcc cagatcccg acttggacg tggaggagg 
      acacccaga gcgaagccc ccgagaatc tctccctca 
      agaagccat ccagtccgg agacacctc cgcaaccga 
      cacagaact tcatccctt accagcagt tcgcatttc 
      ccccctttc agccgcagt accccgccc agcgctcgc 
      ccatcgacg tgctcatga gggtgttcc cggggaaga 
      ggaggtcgg acgtcgagg cccttttgc agaggtgca 
      aaggcgacg tggtgcagg ccatggaga tgatggtga 
      gtgggagcc acgaggacg ccacgccgc catcggcgt 
      tcagtcctc tgggccccc caaccaact tccaccggt 
      tcagccctt cgaggagat tcctgtcgg ctccgtacg 
      ccgggacgg ggtatctcc caacggtga taaggcctc 
      ctcctgatt atctgtcga tggtggggt cggtgcatg 
      atatttaca gcagcgata agacctcgg cgtcttctc 
      cgggctcca acacgagct cggacaaga cgtcgtact 
      cggaatagc cgtgatatt aagtgttta cctgcacgc 
      cctgcatag tggattatt cggtgcgag taatgaaga 
      ccagtcgag tttccgcac gaacaatca catcagaat 
      ttaattata aaatacata tgaatcaag ttccatccc 
      tactcggta ttcagcgac ccctaaccc tattgaata 
      catgaagcc agttattag gtctctatt gttagcctg 
      tagctacca gatcatata aattatatt gtaaacagt 
      ggcgtgtgt tcggtttat tgtctgtat taaagtgtg 
      gtttcattt gtgacgatc gccactggg tccagtgat 
      cacgttgta aataatttt ctactaata ctttagctc 
      cattctatc aaatatgtt gtttcactt gtaaataat 
      tgtgaatag ttttagaca cattcatcg tacaagctg 
      tatattatt aattagatt gtttagagt agttgattg 
      tattaatta aaatggatt gtatacgaa attatataa 
      ataaaataa aatgttaat atagat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY